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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM <t>PF228</t> (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.
Pf573228 Pf228, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pf573228 selleckchem cat  (Selleck Chemicals)
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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM <t>PF228</t> (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.
Pf573228 Selleckchem Cat, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fak inhibitor pf573228  (Selleck Chemicals)
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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM <t>PF228</t> (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.
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pf573228  (Selleck Chemicals)
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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM <t>PF228</t> (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.
Pf573228, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pf573228  (Cayman Chemical)
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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM <t>PF228</t> (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.
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Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM PF228 (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.

Journal: Cell reports

Article Title: Mechanobiological mechanism of cyclic stretch-induced cell columnarization.

doi: 10.1016/j.celrep.2025.115662

Figure Lengend Snippet: Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM PF228 (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.

Article Snippet: MDCK cells were pretreated with 10 μM PF573228 (PF228) (Selleckchem, Houston, TX, USA) for 30 min before undergoing cyclic stretch experiments, during which the cells were continuously exposed to 10 μM PF228 for 24 h. Cell contractility was inhibited by treatment with 50 μM blebbistatin (BB) (Tocris Bioscience, Bristol, UK) for 30 min before and during 24 h of cyclic stretching.

Techniques: Inhibition, Western Blot, Expressing, Solvent, Control

Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM PF228 (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.

Journal: Cell reports

Article Title: Mechanobiological mechanism of cyclic stretch-induced cell columnarization.

doi: 10.1016/j.celrep.2025.115662

Figure Lengend Snippet: Figure 3. Inhibition of focal adhesion proteins partially suppressed CS-induced cell columnarization (A) Western blotting results showing the effects of 10 μM PF228 (FAK inhibitor) treatment for 24 h on pFAK expression in MDCK cells under NS and CS conditions. (B) Representative images of cell junctions (center) and stress fibers (bottom) under NS and CS conditions with and without PF228 treatment. Dimethyl sulfoxide (DMSO) was used as the solvent control of PF228. Scale bar: 20 μm. (C and D) Quantification of the cell height and aspect ratio under NS and CS conditions and treatment with PF228. The quantification was based on mea- surements of 70–83 cells from 3 independent experiments (2-way ANOVA/Bonferroni correction). (E) Transverse and longitudinal sections of vinculin (VCL) expression under NS or CS conditions. Scale bar: 20 μm.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal antiFAK(phosphor Try397) GeneTex Cat#GTX24803; RRID: AB_380431 Mouse monoclonal anti-FAK(clone 77) BD Transduction Cat#610087; RRID: AB_397494 Mouse monoclonal anti-ECadherin(clone 36) BD Transduction Cat#610182; RRID: AB_397581 Rabbit polyclonal anti-ZO-1 Sigma‒Aldrich Cat#SAB5700645 Mouse monoclonal antiGAPDH(clone 1E6D9) Proteintech Cat#60004-I-Ig; RRID: AB_2107436 Goat polyclonal anti-Mouse IgG (H + L)-HRP Croyez Cat#C04001 Goat polyclonal anti-Rabbit IgG (H + L)-HRP Croyez Cat#C04003 Mouse monoclonal antiVinculin(clone hVIN-1) Sigma‒Aldrich Cat#V9131; RRID: AB_477629 Mouse monoclonal anti-αtubulin(clone 1E4C11) Proteintech Cat#66031-I-Ig; RRID: AB_11042766 Rabbit polyclonal anti-Caveolin-1 Abcam Cat#ab18199; RRID: AB_444314 Invitrogen Goat anti-Mouse IgG (H + L) Alexa Fluor 488 Molecular Probes Cat#A11001; RRID: AB_2534069 Invitrogen Goat anti-Mouse IgG (H + L) Alexa Fluor 594 Molecular Probes Cat#A11005; RRID: AB_2534073 Invitrogen Goat anti-Rabbit IgG (H + L) Alexa Fluor 488 Molecular Probes Cat#A11008; RRID: AB_143165 Invitrogen Goat anti-Rabbit IgG (H + L) Alexa Fluor 594 Molecular Probes Cat#A11012; RRID: AB_2534079 Rabbit polyclonal anti-Myosin IIB Sigma‒Aldrich Cat#M7939; RRID: AB_260669 Rabbit monoclonal anti-pMLC2(T18/S19) (clone E2J8F) Cell Signaling Technology Cat#95777; RRID: AB_3677547 Bacterial and virus strains pCMVΔR8.91 RNAi core N/A pMD.G RNAi core N/A Chemicals, peptides, and recombinant proteins Penicillin G sodium salt Sigma‒Aldrich Cat#P3032-25MU; CAS: 69-57-8 Streptomycin sulfate salt Sigma‒Aldrich Cat#3810-74-0; CAS: 3810-74-0 G418 disulfate salt Merck Cat#T1720; CAS: 108321-42-2 PF573228 Selleckchem Cat#S2013; CAS: 869288-64-2 (±)-Blebbistatin Tocris Bioscience Cat#1760; CAS: 674289-55-5 Nocodazole Bio-Techne Cat#1228; CAS: 31430-18-9 Cytochalasin D Merck Cat#C8273; CAS: 22144-77-0 Mitomycin c Sigma‒Aldrich Cat#M4287; CAS: 50-07-7 Thymidine Sigma‒Aldrich Cat#T1895; CAS: 50-89-5 dimethyl sulfoxide (DMSO) Aventor Cat#0231-500ML; CAS: 67-68-5 Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat#L3000015 Puromycin dihydrochloride Sigma‒Aldrich Cat#P8833; CAS: 58-58-2 SuperBlock blocking buffer Thermo Fisher Cat#37516 Phalloidin-TRITC Sigma‒Aldrich Cat#P1951 (Continued on next page) 18 Cell Reports 44, 115662, May 27, 2025

Techniques: Inhibition, Western Blot, Expressing, Solvent, Control