Article Title: CircRNA SOD2 motivates non-small cell lungs cancer advancement with EMT via acting as microRNA-2355-5p’s competing endogenous RNA to mediate calmodulin regulated spectrin associated proteins-2
Figure Lengend Snippet: CircSOD2 performs as miR-2355-5p’s ceRNA. A: RT-qPCR to detect miR-2355-5p after cell transfection; B: Bioinformatics website starbase to predict the binding site of circSOD2 and miR-2355-5p; C: Dual luciferase verification of the binding of circSOD2 to miR-2355-5p; D: RNA pull-down to detect the enrichment of miR-2355-5p on circSOD2; E: RIP assay and qPCR to detect RNA enrichment of circSOD2 and miR-2355-5p in anti-IgG -and anti-Ago2-precipitated RNAs in cell extract of NSCLC cells; F/G: RT-qPCR to detect miR-2355-5p in NSCLC, corresponding adjacent tissues, and 16HBE and HCC827, H1299, H1975, and A549; H: Correlation analysis of circSOD2 and miR-2355-5p in NSCLC tissue; * P
Article Snippet: After blocking with 5% BSA (10-L16, Beijing Zhongsheng Likang Technology Co., Ltd., China), incubation was performed with the primary antibody CAMSAP2 (1: 1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1: 5000) (Cell Signaling Technology, Beverly, MA, USA), E-cadherin (1: 1000), N-cadherin (1: 1000) (Abcam, Cambridge, MA, USA), and horseradish peroxidase-labeled immunoglobulin G (IgG) (1: 1000, Wuhan Boster Company) secondary antibody.
Techniques: Quantitative RT-PCR, Transfection, Binding Assay, Luciferase, Real-time Polymerase Chain Reaction