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Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. <t>ELISA</t> Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease
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Thermo Fisher peroxidase substrate (1-step turbo tmb-elisa, trimethylbenzidine
Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. <t>ELISA</t> Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease
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Thermo Fisher peroxidase substrate trimethylbenzidine
Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. <t>ELISA</t> Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease
Peroxidase Substrate Trimethylbenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxidase substrate trimethylbenzidine - by Bioz Stars, 2026-09
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Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. ELISA Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease

Journal: Alzheimer's Research & Therapy

Article Title: N-truncated Aβ 4–x peptides in sporadic Alzheimer’s disease cases and transgenic Alzheimer mouse models

doi: 10.1186/s13195-017-0309-z

Figure Lengend Snippet: Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. ELISA Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease

Article Snippet: Subsequently, 50 μl of trimethylbenzidine ELISA peroxidase substrate (Interchim, Montluçon, France) was added and incubated for 1–5 minutes at RT in the dark.

Techniques: Enzyme-linked Immunosorbent Assay