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GE Healthcare peroxidase conjugated secondary antibody
Peroxidase Conjugated Secondary Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase conjugated secondary antibody/product/GE Healthcare
Average 92 stars, based on 1208 article reviews
Price from $9.99 to $1999.99
peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Western Blot:

Article Title: Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line
Article Snippet: .. The membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies and then developed using enhanced chemiluminescence plus Western detection kit (Amersham Biosciences). .. The membranes were stripped at 50 °C for 30 min in stripping buffer (62.5 mM Tris–HCl, pH 7.4, 100 mM β-mercaptoethanol, 2% SDS) and re-probed with antibody against β-actin (Santa Cruz Biotechnology), after washing with anti-goat horseradish peroxidase-conjugated secondary antibody (Vector Laboratories).

Incubation:

Article Title: Deficient repair of 8-hydroxyguanine in the BxPC-3 pancreatic cancer cell line
Article Snippet: .. The membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies and then developed using enhanced chemiluminescence plus Western detection kit (Amersham Biosciences). .. The membranes were stripped at 50 °C for 30 min in stripping buffer (62.5 mM Tris–HCl, pH 7.4, 100 mM β-mercaptoethanol, 2% SDS) and re-probed with antibody against β-actin (Santa Cruz Biotechnology), after washing with anti-goat horseradish peroxidase-conjugated secondary antibody (Vector Laboratories).

Article Title: Gap junction proteins in the light-damaged albino rat
Article Snippet: .. Following three washes each of 15 min and one wash for 40 min in TBS-T, the membrane was incubated with a horseradish peroxidase-conjugated secondary antibody (donkey anti-rabbit immunoglobulin G, 1:5,000; Amersham Biosciences, Buckinghamshire, UK) for 1 h at room temperature. ..

Article Title: Downregulation of mTOR by lentivirus inhibits prostate cancer cell growth
Article Snippet: .. Membranes were washed in TBST (3 times for 15 min) and were incubated for 1 h with horseradish peroxidase-conjugated secondary antibodies at a 1:10,000 dilution at room temperature with constant agitation before enhanced chemiluminescence (Amersham Biosciences, NJ) and exposure to film. .. Total RNA from RWPE1, LNCap, PC-3, PC-3m, C4-2, C4-2B and MCF-7 cells was isolated with Trizol reagent (Invitrogen, Carlsbad, CA) as described by the manufacturer.

Article Title: Silencing of Claudin-11 Is Associated with Increased Invasiveness of Gastric Cancer Cells
Article Snippet: .. Blots were then washed and incubated in horseradish peroxidase-conjugated secondary antibody (anti-rabbit IgG: 1∶10,000; Amersham Pharmacia Biotech, Piscataway, NJ). .. For detection, chemiluminescence was carried out using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech).

Article Title: TUMOR-SPECIFIC APOPTOTIC GENE TARGETING OVERCOMES RADIATION RESISTANCE IN ESOPHAGEAL ADENOCARCINOMA
Article Snippet: .. After a second washing with PBS and 0.05% Tween 20, membranes were incubated with peroxidase-conjugated secondary antibodies and developed with an ECL kit (Amersham Bioscience, Buckinghamshire, United Kingdom). ß-actin was used as a loading control. ..

Article Title: Roles of insulin signalling and p38 MAPK in the activation by lithium of glucose transport in insulin-resistant rat skeletal muscle
Article Snippet: .. After incubation with horseradish peroxidase-conjugated secondary antibodies, proteins were visualized by enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ) using the ChemiDoc system (Bio-Rad), and band density was quantified using Quantity One software (Bio-Rad). .. Paired Student's t -tests were employed to determine statistically significant differences in group means when soleus splits derived from the same muscle were used to assess the specific effects of lithium ( - ) or an inhibitor (A304000; and ).

Software:

Article Title: Roles of insulin signalling and p38 MAPK in the activation by lithium of glucose transport in insulin-resistant rat skeletal muscle
Article Snippet: .. After incubation with horseradish peroxidase-conjugated secondary antibodies, proteins were visualized by enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ) using the ChemiDoc system (Bio-Rad), and band density was quantified using Quantity One software (Bio-Rad). .. Paired Student's t -tests were employed to determine statistically significant differences in group means when soleus splits derived from the same muscle were used to assess the specific effects of lithium ( - ) or an inhibitor (A304000; and ).

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  • 88
    GE Healthcare anti mouse secondary antibody conjugated to hrp
    LGG induces ROS that oxidizes cysteines. (A) Intestinal epithelial cells (SKCO15) were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, and then with 15 µM HydroCy3 for 30 min before confocal microscopic analysis at 555 nm, (scale bars 20 µm). (B) SKCO15 were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, labeled for 30 min with a thiol-reactive, Thiol Tracker™ fluorescence probe, and then analyzed by fluorescence microscopy at 405 nm (scale bars 200 µm). Mean image intensity is shown at bottom left for A and B. (C) Biotinylated-iodoacetamide (BIAM) labeling of cysteine residues in lysates of LGG or E. coli contacted SKCO15 cells, followed by pull-down of labeled residues with <t>streptavidin</t> conjugated agarose and detected by Western blot using <t>HRP</t> conjugated streptavidin as a probe. The relative intensity of each lane of the blot is shown in arbitrary units to the left. Each value was normalized to calnexin that served as a loading control to give the relative oxidation amounts. (D) Dual labeling of LGG or E. coli contacted (15 mins) SKCO15 cultured cells with HydroCy3 (red) and Lysotracker (green). Note co-localization of lysotracker and hydro-Cy3 in LGG contacted cells (bars 10 µm).
    Anti Mouse Secondary Antibody Conjugated To Hrp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse secondary antibody conjugated to hrp/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse secondary antibody conjugated to hrp - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    GE Healthcare secondary horseradish peroxidase conjugated donkey anti rabbit igg
    The single alanine substitutions of the key amino acid residues within the NR2B and NR1 M3 domains contribute to the regulation of the trafficking of the functional receptors. A, the amino acid sequences of the NR2B and NR1 M3 domains are shown, and the amino acid residues replaced with alanines are underlined. B and C, COS-7 cells cotransfected with indicated NR1/NR2B subunits were solubilized with 1% deoxycholate, immunoprecipitated ( IP ) with mouse anti-NR1 ( B ) or anti-MYC ( C ) antibodies ( Ab ), and probed with rabbit <t>anti-GFP</t> antibody. The specificity of coimmunoprecipitation was tested by using <t>IgG.</t> The images show the representative results from three independent experiments. Densitometric analysis revealed no significant differences in the normalized amounts of the bound fractions among the studied combinations of the subunits (ratio between YFP-NR1-1a-W636A-Y647A-T648A/MYC-NR2B and YFP-NR1-1a/MYC-NR2B, 1.07 ± 0.12; ratio between NR1-1a/GFP-NR2B-W635A-S645A-Y646A-T647A and NR1-1a/GFP-NR2B, 1.09 ± 0.04; n = 3). D and E, the distribution of indicated mutated NMDA receptors closely matches the distribution of an ER marker ( D ) but not a GA marker ( E ). Images were taken on fixed COS-7 cells using a confocal microscope. PDI , oxidoreductase-protein disulfide isomerase.
    Secondary Horseradish Peroxidase Conjugated Donkey Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary horseradish peroxidase conjugated donkey anti rabbit igg/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary horseradish peroxidase conjugated donkey anti rabbit igg - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    85
    GE Healthcare horse raddish peroxidase hrp conjugated secondary antibodies sheep anti mouse igg
    Degradation of deposited C3b by B. anthracis spores-bound PLG. (A) Spores (2×10 7 /well) were immobilized onto a microplate and incubated with 10% NHS (100 µl) for 30 min at room temperature. Washed spores were incubated with 2 µg/well PLG for 1 h in the presence or absence of protease cocktail. Bound PLG was activated by uPA (20 units/well) for 3 h at 37°C. Deposited C3b was detected by anti-C3c <t>IgG</t> and <t>HRP-conjugated</t> secondary antibody followed by TMB reaction. C3b deposition is expressed as the mean absorbance at 450 nm of quadruplicates. Error bars indicate ± standard deviation. *P
    Horse Raddish Peroxidase Hrp Conjugated Secondary Antibodies Sheep Anti Mouse Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse raddish peroxidase hrp conjugated secondary antibodies sheep anti mouse igg/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse raddish peroxidase hrp conjugated secondary antibodies sheep anti mouse igg - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    GE Healthcare horseradish peroxidase conjugated secondary antibody
    α-actin is serotonylated in aortic smooth muscle cells and inhibition of TG activity reduces aortic contraction to 5-HT. A. Immunoprecipitation of smooth muscle α-actin from rat aortic homogenates exposed to 5-HT-biotin in a standard transglutaminase reaction. Blots were developed using a streptavidin <t>secondary</t> (top), or exposed to a primary <t>antibody</t> against α-actin (bottom) and developed using standard <t>horseradish</t> <t>peroxidase</t> secondary antibody. Representative of N = 6 different experiments. B. Effect of vehicle (filled symbol) and cystamine (0.1–1 mM; open symbol) on 5-HT (top) and KCl (bottom)-induced contraction in isolated rat aorta. * indicates statistical difference from vehicle-incubated values. Points and vertical lines represent means±SEM for number of animals in parentheses.
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/GE Healthcare
    Average 92 stars, based on 614 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    LGG induces ROS that oxidizes cysteines. (A) Intestinal epithelial cells (SKCO15) were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, and then with 15 µM HydroCy3 for 30 min before confocal microscopic analysis at 555 nm, (scale bars 20 µm). (B) SKCO15 were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, labeled for 30 min with a thiol-reactive, Thiol Tracker™ fluorescence probe, and then analyzed by fluorescence microscopy at 405 nm (scale bars 200 µm). Mean image intensity is shown at bottom left for A and B. (C) Biotinylated-iodoacetamide (BIAM) labeling of cysteine residues in lysates of LGG or E. coli contacted SKCO15 cells, followed by pull-down of labeled residues with streptavidin conjugated agarose and detected by Western blot using HRP conjugated streptavidin as a probe. The relative intensity of each lane of the blot is shown in arbitrary units to the left. Each value was normalized to calnexin that served as a loading control to give the relative oxidation amounts. (D) Dual labeling of LGG or E. coli contacted (15 mins) SKCO15 cultured cells with HydroCy3 (red) and Lysotracker (green). Note co-localization of lysotracker and hydro-Cy3 in LGG contacted cells (bars 10 µm).

    Journal: Redox Biology

    Article Title: Proteomic analysis of microbial induced redox-dependent intestinal signaling

    doi: 10.1016/j.redox.2018.11.011

    Figure Lengend Snippet: LGG induces ROS that oxidizes cysteines. (A) Intestinal epithelial cells (SKCO15) were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, and then with 15 µM HydroCy3 for 30 min before confocal microscopic analysis at 555 nm, (scale bars 20 µm). (B) SKCO15 were contacted with 10 8 CFU/mL of E. coli or LGG for 15 min, labeled for 30 min with a thiol-reactive, Thiol Tracker™ fluorescence probe, and then analyzed by fluorescence microscopy at 405 nm (scale bars 200 µm). Mean image intensity is shown at bottom left for A and B. (C) Biotinylated-iodoacetamide (BIAM) labeling of cysteine residues in lysates of LGG or E. coli contacted SKCO15 cells, followed by pull-down of labeled residues with streptavidin conjugated agarose and detected by Western blot using HRP conjugated streptavidin as a probe. The relative intensity of each lane of the blot is shown in arbitrary units to the left. Each value was normalized to calnexin that served as a loading control to give the relative oxidation amounts. (D) Dual labeling of LGG or E. coli contacted (15 mins) SKCO15 cultured cells with HydroCy3 (red) and Lysotracker (green). Note co-localization of lysotracker and hydro-Cy3 in LGG contacted cells (bars 10 µm).

    Article Snippet: Anti-mouse secondary antibody conjugated to HRP was obtained from GE, while the streptavidin conjugated HRP was obtained from Abcam.

    Techniques: Labeling, Fluorescence, Microscopy, Western Blot, Cell Culture

    The single alanine substitutions of the key amino acid residues within the NR2B and NR1 M3 domains contribute to the regulation of the trafficking of the functional receptors. A, the amino acid sequences of the NR2B and NR1 M3 domains are shown, and the amino acid residues replaced with alanines are underlined. B and C, COS-7 cells cotransfected with indicated NR1/NR2B subunits were solubilized with 1% deoxycholate, immunoprecipitated ( IP ) with mouse anti-NR1 ( B ) or anti-MYC ( C ) antibodies ( Ab ), and probed with rabbit anti-GFP antibody. The specificity of coimmunoprecipitation was tested by using IgG. The images show the representative results from three independent experiments. Densitometric analysis revealed no significant differences in the normalized amounts of the bound fractions among the studied combinations of the subunits (ratio between YFP-NR1-1a-W636A-Y647A-T648A/MYC-NR2B and YFP-NR1-1a/MYC-NR2B, 1.07 ± 0.12; ratio between NR1-1a/GFP-NR2B-W635A-S645A-Y646A-T647A and NR1-1a/GFP-NR2B, 1.09 ± 0.04; n = 3). D and E, the distribution of indicated mutated NMDA receptors closely matches the distribution of an ER marker ( D ) but not a GA marker ( E ). Images were taken on fixed COS-7 cells using a confocal microscope. PDI , oxidoreductase-protein disulfide isomerase.

    Journal: The Journal of Biological Chemistry

    Article Title: Key Amino Acid Residues within the Third Membrane Domains of NR1 and NR2 Subunits Contribute to the Regulation of the Surface Delivery of N-methyl-d-aspartate Receptors *

    doi: 10.1074/jbc.M112.339085

    Figure Lengend Snippet: The single alanine substitutions of the key amino acid residues within the NR2B and NR1 M3 domains contribute to the regulation of the trafficking of the functional receptors. A, the amino acid sequences of the NR2B and NR1 M3 domains are shown, and the amino acid residues replaced with alanines are underlined. B and C, COS-7 cells cotransfected with indicated NR1/NR2B subunits were solubilized with 1% deoxycholate, immunoprecipitated ( IP ) with mouse anti-NR1 ( B ) or anti-MYC ( C ) antibodies ( Ab ), and probed with rabbit anti-GFP antibody. The specificity of coimmunoprecipitation was tested by using IgG. The images show the representative results from three independent experiments. Densitometric analysis revealed no significant differences in the normalized amounts of the bound fractions among the studied combinations of the subunits (ratio between YFP-NR1-1a-W636A-Y647A-T648A/MYC-NR2B and YFP-NR1-1a/MYC-NR2B, 1.07 ± 0.12; ratio between NR1-1a/GFP-NR2B-W635A-S645A-Y646A-T647A and NR1-1a/GFP-NR2B, 1.09 ± 0.04; n = 3). D and E, the distribution of indicated mutated NMDA receptors closely matches the distribution of an ER marker ( D ) but not a GA marker ( E ). Images were taken on fixed COS-7 cells using a confocal microscope. PDI , oxidoreductase-protein disulfide isomerase.

    Article Snippet: Proteins were loaded onto 7% polyacrylamide gels, transferred to polyvinylidene difluoride membranes, incubated with rabbit primary anti-GFP (Millipore, 1:1000) and secondary horseradish peroxidase-conjugated donkey anti-rabbit IgG (GE Healthcare, 1:5000) antibodies, and detected with ECL using BioMax MR x-ray films (Eastman Kodak, Rochester, NY).

    Techniques: Functional Assay, Immunoprecipitation, Marker, Microscopy

    Degradation of deposited C3b by B. anthracis spores-bound PLG. (A) Spores (2×10 7 /well) were immobilized onto a microplate and incubated with 10% NHS (100 µl) for 30 min at room temperature. Washed spores were incubated with 2 µg/well PLG for 1 h in the presence or absence of protease cocktail. Bound PLG was activated by uPA (20 units/well) for 3 h at 37°C. Deposited C3b was detected by anti-C3c IgG and HRP-conjugated secondary antibody followed by TMB reaction. C3b deposition is expressed as the mean absorbance at 450 nm of quadruplicates. Error bars indicate ± standard deviation. *P

    Journal: PLoS ONE

    Article Title: Bacillus anthracis Interacts with Plasmin(ogen) to Evade C3b-Dependent Innate Immunity

    doi: 10.1371/journal.pone.0018119

    Figure Lengend Snippet: Degradation of deposited C3b by B. anthracis spores-bound PLG. (A) Spores (2×10 7 /well) were immobilized onto a microplate and incubated with 10% NHS (100 µl) for 30 min at room temperature. Washed spores were incubated with 2 µg/well PLG for 1 h in the presence or absence of protease cocktail. Bound PLG was activated by uPA (20 units/well) for 3 h at 37°C. Deposited C3b was detected by anti-C3c IgG and HRP-conjugated secondary antibody followed by TMB reaction. C3b deposition is expressed as the mean absorbance at 450 nm of quadruplicates. Error bars indicate ± standard deviation. *P

    Article Snippet: Horse raddish peroxidase (HRP)-conjugated secondary antibodies sheep anti-mouse IgG and donkey anti-rabbit IgG antibody were purchased from GE Healthcare and rabbit anti-goat IgG antibody from Jackson Immuno Research.

    Techniques: Incubation, Standard Deviation

    α-actin is serotonylated in aortic smooth muscle cells and inhibition of TG activity reduces aortic contraction to 5-HT. A. Immunoprecipitation of smooth muscle α-actin from rat aortic homogenates exposed to 5-HT-biotin in a standard transglutaminase reaction. Blots were developed using a streptavidin secondary (top), or exposed to a primary antibody against α-actin (bottom) and developed using standard horseradish peroxidase secondary antibody. Representative of N = 6 different experiments. B. Effect of vehicle (filled symbol) and cystamine (0.1–1 mM; open symbol) on 5-HT (top) and KCl (bottom)-induced contraction in isolated rat aorta. * indicates statistical difference from vehicle-incubated values. Points and vertical lines represent means±SEM for number of animals in parentheses.

    Journal: PLoS ONE

    Article Title: Serotonylation of Vascular Proteins Important to Contraction

    doi: 10.1371/journal.pone.0005682

    Figure Lengend Snippet: α-actin is serotonylated in aortic smooth muscle cells and inhibition of TG activity reduces aortic contraction to 5-HT. A. Immunoprecipitation of smooth muscle α-actin from rat aortic homogenates exposed to 5-HT-biotin in a standard transglutaminase reaction. Blots were developed using a streptavidin secondary (top), or exposed to a primary antibody against α-actin (bottom) and developed using standard horseradish peroxidase secondary antibody. Representative of N = 6 different experiments. B. Effect of vehicle (filled symbol) and cystamine (0.1–1 mM; open symbol) on 5-HT (top) and KCl (bottom)-induced contraction in isolated rat aorta. * indicates statistical difference from vehicle-incubated values. Points and vertical lines represent means±SEM for number of animals in parentheses.

    Article Snippet: Samples were blocked overnight at 4°C in 4% chick egg ovalbulmin [TBS-0.1% Tween+0.025% NaN3, ], washed in TBS-Tween for 20 minutes, and incubated with streptavidin-linked, horseradish peroxidase-conjugated secondary antibody (1∶2000, 1 hr, 4°C GE Healthcare, Piscataway NJ, USA).

    Techniques: Inhibition, Activity Assay, Immunoprecipitation, Isolation, Incubation