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Cell Signaling Technology Inc peroxidase conjugated secondary antibody
Peroxidase Conjugated Secondary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase conjugated secondary antibody/product/Cell Signaling Technology Inc
Average 99 stars, based on 451 article reviews
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peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
99/100 stars

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Western Blot:

Article Title: An essential role for maternal control of Nodal signaling
Article Snippet: .. Anti-eIF4E (1:2000; #2067, Cell Signaling Technology) and anti-eIF4G (1:2000; #2469, Cell Signaling Technology) antibodies were used in co-immunoprecipitation assays and western blots to detect interactions with Ybx1. .. Semi-quantitative and quantitative RT-PCR Total RNA was extracted from embryos using TRIzol reagent (Invitrogen), and 500 ng RNA from WT, Pybx1 sa42 or Mybx1 sa42 embryos was used for first-strand cDNA synthesis.

Article Title: NEDDylation promotes nuclear protein aggregation and protects the Ubiquitin Proteasome System upon proteotoxic stress
Article Snippet: .. Rabbit monoclonal anti-NEDD8 (1:2000), Y297 (GeneTex, GTX61205), FK2 mouse anti-ubiquitin, stainings (1:250) (Viva Bioscience, VB2500), rabbit anti-ubiquitin (1:2000), western blotting (DAKO, Z0458), mouse anti-fibrilarin (1:1000) (ab4566), rabbit anti-nucleolin (1:1000) (ab22758), mouse anti-GAPDH (1:5000) (6C5, ab8245), rabbit anti-RPL7 (1:2000) (ab72550) (Abcam), mouse anti-tubulin (1:2000) (Cell Signalling, 3873), mouse anti-HA (1:2000) (12C5, 11583816001), mouse anti-GFP (1:500) (11814460001) (Roche), mouse anti-a6 proteasome subunit (1 μg/ml) (Enzo Life Sciences, BML-PW8100), rabbit polyclonal anti-HUWE1 (1:2000) (Bethyl laboratories, A300-486A), mouse monoclonal anti-p21 (1 μg/ml) (F-5, sc-6246, Santa Cruz), rabbit polyclonal anti-CDT1 (1:1000) (# 06-1295, Millipore), goat anti-mouse Alexa Fluor® 488 (115-545-146), goat Anti-Rabbit Alexa Fluor® 594 (111-585-008) (Jackson ImmunoResearch). .. Transfections Cells were seeded in six-well plates or 10 cm dishes to the desired confluency.

Incubation:

Article Title: Geraniin inhibits TNF-α-induced impairments of osteogenesis through NF-κB and p38 MAPK signalling pathways in bone marrow stem cells
Article Snippet: .. Thirty microgram proteins were separated using 10%–12% SDS-PAGE (Beyotime Institute of Biotechnology, China), transferred onto polyvinylidene difluoride membranes, blocked with 5% skimmed milk in TBS–Tween (0.2% Tween-20) for 2 hour at 37°C and then incubated with primary antibodies overnight at 4°C including anti-NF-κB/p65 (1:2000, Cell Signaling Technology, USA), anti-IкB-α (1:2000, Cell Signaling Technology, USA), anti-p38 MAPK(1:2000, Cell Signaling Technology, USA) and β-actin (Beyotime Institute of Biotechnology, China). .. Membranes were incubated with the appropriate secondary antibodies (Beyotime Institute of Biotechnology, China) conjugated with an IRDye 800CW.

Article Title: Mitochondria-associated lactate dehydrogenase is not a biologically significant contributor to bioenergetic function in murine striated muscle
Article Snippet: .. After blocking in 5% milk, the membranes were incubated with antibodies against LDHB (1:2000, Abcam), GAPDH with HRP-conjugated (1:4000, Cell Signaling), ALDH2 (1:4000, Invitrogen), or HSP60 (1:2000, Cell Signaling) overnight at 4 °C. .. After incubation with HRP-linked secondary antibodies (anti-mouse, 1:2500, Cell Signaling; anti-rabbit, 1:2500, Cell Signaling; and anti-goat, 1:2500; Invitrogen), the membranes were developed using Pierce™ ECL Western Blotting Substrate (ThermoFisher).

Article Title: Tubeimoside I Protects Dopaminergic Neurons Against Inflammation-Mediated Damage in Lipopolysaccharide (LPS)-Evoked Model of Parkinson’s Disease in Rats
Article Snippet: .. After blocking with 5% skim milk, the polyvinylidene difluoride membranes were incubated with primary antibodies against TH (1:1000) (Abcam, Cambridge, UK), OX-42 (1:1000) (Abcam), COX-2 (1:2000) (Abcam), iNOS (1:2000) (Abcam), phospho-AKT (1:2000) (Cell Signaling Technology, MA, USA), phospho-JNK1/2 (1:2000) (Cell Signaling Technology), phospho-ERK1/2 (1:2000) (Cell Signaling Technology), phospho-p38 (1:2,000), phospho-NF-κB p65 (1:2000), JNK1/2 (1:2000), ERK1/2 (1:22,000) (Cell Signaling Technology), p38 (1:2000) (Cell Signaling Technology), AKT (1:2000) (Cell Signaling Technology), NF-κB p65 (1:2000) (Cell Signaling Technology) and β-actin (1:10,000) (Santa Cruz, CA, USA). .. Then the polyvinylidene difluoride membranes were incubated at room temperature for 1 h against the secondary antibodies goat anti-rabbit (1:2000) or goat anti-mouse (1:2000).

other:

Article Title: Suppression of GSK-3? activation by M-cadherin protects myoblasts against mitochondria-associated apoptosis during myogenic differentiation
Article Snippet: Antibodies specific to phosphorylated Ser473 Akt, total Akt, phosphorylated Ser9 GSK3β, total GSK3β, cytochrome c , cleaved caspase-9 and cleaved caspase-3, AIF, survivin (1:1000) and cyclin D1 (1:2000 dilution) were purchased from Cell Signaling Technology (Danvers, MA).

SDS Page:

Article Title: Geraniin inhibits TNF-α-induced impairments of osteogenesis through NF-κB and p38 MAPK signalling pathways in bone marrow stem cells
Article Snippet: .. Thirty microgram proteins were separated using 10%–12% SDS-PAGE (Beyotime Institute of Biotechnology, China), transferred onto polyvinylidene difluoride membranes, blocked with 5% skimmed milk in TBS–Tween (0.2% Tween-20) for 2 hour at 37°C and then incubated with primary antibodies overnight at 4°C including anti-NF-κB/p65 (1:2000, Cell Signaling Technology, USA), anti-IкB-α (1:2000, Cell Signaling Technology, USA), anti-p38 MAPK(1:2000, Cell Signaling Technology, USA) and β-actin (Beyotime Institute of Biotechnology, China). .. Membranes were incubated with the appropriate secondary antibodies (Beyotime Institute of Biotechnology, China) conjugated with an IRDye 800CW.

Blocking Assay:

Article Title: Mitochondria-associated lactate dehydrogenase is not a biologically significant contributor to bioenergetic function in murine striated muscle
Article Snippet: .. After blocking in 5% milk, the membranes were incubated with antibodies against LDHB (1:2000, Abcam), GAPDH with HRP-conjugated (1:4000, Cell Signaling), ALDH2 (1:4000, Invitrogen), or HSP60 (1:2000, Cell Signaling) overnight at 4 °C. .. After incubation with HRP-linked secondary antibodies (anti-mouse, 1:2500, Cell Signaling; anti-rabbit, 1:2500, Cell Signaling; and anti-goat, 1:2500; Invitrogen), the membranes were developed using Pierce™ ECL Western Blotting Substrate (ThermoFisher).

Article Title: Tubeimoside I Protects Dopaminergic Neurons Against Inflammation-Mediated Damage in Lipopolysaccharide (LPS)-Evoked Model of Parkinson’s Disease in Rats
Article Snippet: .. After blocking with 5% skim milk, the polyvinylidene difluoride membranes were incubated with primary antibodies against TH (1:1000) (Abcam, Cambridge, UK), OX-42 (1:1000) (Abcam), COX-2 (1:2000) (Abcam), iNOS (1:2000) (Abcam), phospho-AKT (1:2000) (Cell Signaling Technology, MA, USA), phospho-JNK1/2 (1:2000) (Cell Signaling Technology), phospho-ERK1/2 (1:2000) (Cell Signaling Technology), phospho-p38 (1:2,000), phospho-NF-κB p65 (1:2000), JNK1/2 (1:2000), ERK1/2 (1:22,000) (Cell Signaling Technology), p38 (1:2000) (Cell Signaling Technology), AKT (1:2000) (Cell Signaling Technology), NF-κB p65 (1:2000) (Cell Signaling Technology) and β-actin (1:10,000) (Santa Cruz, CA, USA). .. Then the polyvinylidene difluoride membranes were incubated at room temperature for 1 h against the secondary antibodies goat anti-rabbit (1:2000) or goat anti-mouse (1:2000).

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  • 85
    Cell Signaling Technology Inc ac3 expression
    Ketamine induces activated caspase-3 <t>(AC3):</t> Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p
    Ac3 Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ac3 expression/product/Cell Signaling Technology Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    91
    Cell Signaling Technology Inc peroxidase conjugated goat anti β actin secondary antibodies
    Timeline of the experimental design and confirmation of the effectiveness of the IL-1β shRNA lentivirus in mice. a Experimental procedure for the test schedule. NS shRNA or IL-1β shRNA were microinfused into bilateral DG regions of the hippocampus of mice, followed by a 7-day recovery. LPS (1 mg/kg, i.p.) or its vehicle was administered 7 days after the viral infusions (day 0), and then, 24 h later, the OFT, NORT, EZM, FST, and SPT were conducted. b NS shRNA or IL-1β shRNA were well expressed in the HEK293 cells and at the hippocampal microinjection sites in the DG regions of the hippocampus, as indicated by GFP (green) under fluorescence microscopy. Scale bars = 100 μm (HEK293 cell) or 200 μm (hippocampus). c – f The expressions of GFP ( c , d ) and IL-1β ( e , f ) in the DG regions of the hippocampus were shown and normalized by the level of <t>β-actin.</t> The data are expressed as the mean ± S.E.M ( n = 3 per group for western blotting). * p
    Peroxidase Conjugated Goat Anti β Actin Secondary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti β actin secondary antibodies/product/Cell Signaling Technology Inc
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    93
    Cell Signaling Technology Inc anti mouse gfap horseradish peroxidase conjugated secondary antibodies
    <t>GFAP</t> and Nf-κB levels increase in glucose-treated retinas. (A) Western blot probed with antibodies against GFAP (top) and <t>RelA</t> (Nf-κB, middle). β-actin (bottom) served as the loading control. (B,C) Densitometric analysis identified increases in mean GFAP (B) and Nf-κB (C) content in glucose-treated retinal homogenates compared with water-treated or mannitol-treated preparations. C, water-treated control; G, glucose treated; M, mannitol treated. C2, G2 and M2 represent replicates for the C, G and M groups. Error bars represent s.e.m. The asterisk in C indicates that differences in RelA levels were significant across all 3 treatment groups ( P ≤0.003).
    Anti Mouse Gfap Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse gfap horseradish peroxidase conjugated secondary antibodies/product/Cell Signaling Technology Inc
    Average 93 stars, based on 3 article reviews
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    94
    Cell Signaling Technology Inc horseradish peroxidase hrp conjugated secondary antibody
    Oncogenic GNAQ Q209P -mediated activation of ERK and YAP signalling in choroidal melanocytes at the junction between RPE and choroid A-D. Transverse sections of formalin-fixed and paraffin-embedded eye tissues of F 1 generation, 5-month-old Tg ( mitfa :GNAQ Q209P ) zebrafish. (A) H E staining demonstrating choroidal hyperplasia (black arrowheads). White dashed box indicates the region of the choroid magnified in B-D. (B-D) Transverse sections of formalin-fixed and paraffin-embedded eye tissues were stained by IHC, visualized by ImmPact NovaRed peroxidase <t>(HRP)</t> substrate then counterstained with hematoxylin (blue). (B) Negative control: section incubated with <t>1x</t> PBS instead of primary antibody. (C) Immunoreactivity to pERK1/2 (read-out of ERK activation; black arrowheads) in melanocytes at the interface between the RPE and choroid. (D) YAP-positive nuclei (read-out of YAP activation; black arrowheads) in the same cells. Scale bars, 20 μm.
    Horseradish Peroxidase Hrp Conjugated Secondary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated secondary antibody/product/Cell Signaling Technology Inc
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    Image Search Results


    Ketamine induces activated caspase-3 (AC3): Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine induces activated caspase-3 (AC3): Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Western Blot, Injection, Expressing

    Ketamine induces changes in core body temperature (CBT) and activated caspase-3 (AC3) expression in P7 neonates A. CBT (mean ± SE) was measured at various times (0-8 hours) following injection of vehicle (V; n = 7), MK801 (1 mg/kg; n = 4) or ketamine (2-20 mg/kg; K2-K20; n = 4-5). B. Quantitative analysis of AC3 positive cells (mean ± SE) in layers IV and V of the anterior somatosensory cortex across all treatments (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; ***p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine induces changes in core body temperature (CBT) and activated caspase-3 (AC3) expression in P7 neonates A. CBT (mean ± SE) was measured at various times (0-8 hours) following injection of vehicle (V; n = 7), MK801 (1 mg/kg; n = 4) or ketamine (2-20 mg/kg; K2-K20; n = 4-5). B. Quantitative analysis of AC3 positive cells (mean ± SE) in layers IV and V of the anterior somatosensory cortex across all treatments (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; ***p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Expressing, Injection

    Elevation of CBT does not alter ketamine-induced apoptosis in layers IV and V of the somatosensory cortex in P7 rats Animals were injected with vehicle or 40 mg/kg ketamine (K40) as described (see Experimental Procedures). For some K40 animals, CBT was not artificially maintained (K40-HT) whereas for other K40 animals CBT was artificially maintained (K40-NT; see Experimental Procedures) A. Changes in CBT (mean ± SE) after injection of vehicle (V; n = 7) or 40 mg/kg ketamine in the absence of presence of an artificial heat source (K40-HT, n = 4 or K40-NT, n = 4). B. Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Elevation of CBT does not alter ketamine-induced apoptosis in layers IV and V of the somatosensory cortex in P7 rats Animals were injected with vehicle or 40 mg/kg ketamine (K40) as described (see Experimental Procedures). For some K40 animals, CBT was not artificially maintained (K40-HT) whereas for other K40 animals CBT was artificially maintained (K40-NT; see Experimental Procedures) A. Changes in CBT (mean ± SE) after injection of vehicle (V; n = 7) or 40 mg/kg ketamine in the absence of presence of an artificial heat source (K40-HT, n = 4 or K40-NT, n = 4). B. Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Injection

    Ketamine-induced changes in CBT are age-dependent A. CBT changes (mean ± SE) were measured in P14 and P21 animals after injection of vehicle (V; n = 2 both ages) or ketamine (K40; n = 3 both ages). B Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures) in P14 and P21 animals. In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine-induced changes in CBT are age-dependent A. CBT changes (mean ± SE) were measured in P14 and P21 animals after injection of vehicle (V; n = 2 both ages) or ketamine (K40; n = 3 both ages). B Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures) in P14 and P21 animals. In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Injection

    Timeline of the experimental design and confirmation of the effectiveness of the IL-1β shRNA lentivirus in mice. a Experimental procedure for the test schedule. NS shRNA or IL-1β shRNA were microinfused into bilateral DG regions of the hippocampus of mice, followed by a 7-day recovery. LPS (1 mg/kg, i.p.) or its vehicle was administered 7 days after the viral infusions (day 0), and then, 24 h later, the OFT, NORT, EZM, FST, and SPT were conducted. b NS shRNA or IL-1β shRNA were well expressed in the HEK293 cells and at the hippocampal microinjection sites in the DG regions of the hippocampus, as indicated by GFP (green) under fluorescence microscopy. Scale bars = 100 μm (HEK293 cell) or 200 μm (hippocampus). c – f The expressions of GFP ( c , d ) and IL-1β ( e , f ) in the DG regions of the hippocampus were shown and normalized by the level of β-actin. The data are expressed as the mean ± S.E.M ( n = 3 per group for western blotting). * p

    Journal: Journal of Neuroinflammation

    Article Title: Lentivirus-mediated interleukin-1β (IL-1β) knock-down in the hippocampus alleviates lipopolysaccharide (LPS)-induced memory deficits and anxiety- and depression-like behaviors in mice

    doi: 10.1186/s12974-017-0964-9

    Figure Lengend Snippet: Timeline of the experimental design and confirmation of the effectiveness of the IL-1β shRNA lentivirus in mice. a Experimental procedure for the test schedule. NS shRNA or IL-1β shRNA were microinfused into bilateral DG regions of the hippocampus of mice, followed by a 7-day recovery. LPS (1 mg/kg, i.p.) or its vehicle was administered 7 days after the viral infusions (day 0), and then, 24 h later, the OFT, NORT, EZM, FST, and SPT were conducted. b NS shRNA or IL-1β shRNA were well expressed in the HEK293 cells and at the hippocampal microinjection sites in the DG regions of the hippocampus, as indicated by GFP (green) under fluorescence microscopy. Scale bars = 100 μm (HEK293 cell) or 200 μm (hippocampus). c – f The expressions of GFP ( c , d ) and IL-1β ( e , f ) in the DG regions of the hippocampus were shown and normalized by the level of β-actin. The data are expressed as the mean ± S.E.M ( n = 3 per group for western blotting). * p

    Article Snippet: Afterward, the membranes were incubated with horseradish peroxidase-conjugated goat anti-β-actin secondary antibodies (1:1000; Cell Signaling Technology, MA, USA) for 60 min.

    Techniques: shRNA, Mouse Assay, Single-particle Tracking, Fluorescence, Microscopy, Western Blot

    GFAP and Nf-κB levels increase in glucose-treated retinas. (A) Western blot probed with antibodies against GFAP (top) and RelA (Nf-κB, middle). β-actin (bottom) served as the loading control. (B,C) Densitometric analysis identified increases in mean GFAP (B) and Nf-κB (C) content in glucose-treated retinal homogenates compared with water-treated or mannitol-treated preparations. C, water-treated control; G, glucose treated; M, mannitol treated. C2, G2 and M2 represent replicates for the C, G and M groups. Error bars represent s.e.m. The asterisk in C indicates that differences in RelA levels were significant across all 3 treatment groups ( P ≤0.003).

    Journal: Disease Models & Mechanisms

    Article Title: One month of hyperglycemia alters spectral responses of the zebrafish photopic electroretinogram

    doi: 10.1242/dmm.035220

    Figure Lengend Snippet: GFAP and Nf-κB levels increase in glucose-treated retinas. (A) Western blot probed with antibodies against GFAP (top) and RelA (Nf-κB, middle). β-actin (bottom) served as the loading control. (B,C) Densitometric analysis identified increases in mean GFAP (B) and Nf-κB (C) content in glucose-treated retinal homogenates compared with water-treated or mannitol-treated preparations. C, water-treated control; G, glucose treated; M, mannitol treated. C2, G2 and M2 represent replicates for the C, G and M groups. Error bars represent s.e.m. The asterisk in C indicates that differences in RelA levels were significant across all 3 treatment groups ( P ≤0.003).

    Article Snippet: Anti-rabbit (RelA, β-actin) or anti-mouse (GFAP) horseradish peroxidase-conjugated secondary antibodies were applied at a dilution of 1:2000 (Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Western Blot

    Oncogenic GNAQ Q209P -mediated activation of ERK and YAP signalling in choroidal melanocytes at the junction between RPE and choroid A-D. Transverse sections of formalin-fixed and paraffin-embedded eye tissues of F 1 generation, 5-month-old Tg ( mitfa :GNAQ Q209P ) zebrafish. (A) H E staining demonstrating choroidal hyperplasia (black arrowheads). White dashed box indicates the region of the choroid magnified in B-D. (B-D) Transverse sections of formalin-fixed and paraffin-embedded eye tissues were stained by IHC, visualized by ImmPact NovaRed peroxidase (HRP) substrate then counterstained with hematoxylin (blue). (B) Negative control: section incubated with 1x PBS instead of primary antibody. (C) Immunoreactivity to pERK1/2 (read-out of ERK activation; black arrowheads) in melanocytes at the interface between the RPE and choroid. (D) YAP-positive nuclei (read-out of YAP activation; black arrowheads) in the same cells. Scale bars, 20 μm.

    Journal: Oncotarget

    Article Title: Minimal contribution of ERK1/2-MAPK signalling towards the maintenance of oncogenic GNAQQ209P-driven uveal melanomas in zebrafish

    doi: 10.18632/oncotarget.9207

    Figure Lengend Snippet: Oncogenic GNAQ Q209P -mediated activation of ERK and YAP signalling in choroidal melanocytes at the junction between RPE and choroid A-D. Transverse sections of formalin-fixed and paraffin-embedded eye tissues of F 1 generation, 5-month-old Tg ( mitfa :GNAQ Q209P ) zebrafish. (A) H E staining demonstrating choroidal hyperplasia (black arrowheads). White dashed box indicates the region of the choroid magnified in B-D. (B-D) Transverse sections of formalin-fixed and paraffin-embedded eye tissues were stained by IHC, visualized by ImmPact NovaRed peroxidase (HRP) substrate then counterstained with hematoxylin (blue). (B) Negative control: section incubated with 1x PBS instead of primary antibody. (C) Immunoreactivity to pERK1/2 (read-out of ERK activation; black arrowheads) in melanocytes at the interface between the RPE and choroid. (D) YAP-positive nuclei (read-out of YAP activation; black arrowheads) in the same cells. Scale bars, 20 μm.

    Article Snippet: To remove excess unbound primary antibody, membranes were washed three times with 1x TBST buffer then incubated with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signalling Technology, 1:1000 dilution) for 1 hour at room temperature.

    Techniques: Activation Assay, Staining, Immunohistochemistry, Negative Control, Incubation