peroxidase conjugated secondary antibodies  (Thermo Fisher)


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    Structured Review

    Thermo Fisher peroxidase conjugated secondary antibodies
    HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by <t>HRP-conjugated</t> <t>anti-mouse</t> <t>secondary</t> antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .
    Peroxidase Conjugated Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies/product/Thermo Fisher
    Average 99 stars, based on 815 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry"

    Article Title: Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry

    Journal: bioRxiv

    doi: 10.1101/858472

    HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .
    Figure Legend Snippet: HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .

    Techniques Used:

    2) Product Images from "Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry"

    Article Title: Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry

    Journal: bioRxiv

    doi: 10.1101/858472

    HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .
    Figure Legend Snippet: HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .

    Techniques Used:

    Related Articles

    Transduction:

    Article Title: Cortactin Is Necessary for F-Actin Accumulation in Pedestal Structures Induced by Enteropathogenic Escherichia coli Infection
    Article Snippet: .. The membrane-bound protein was probed with the anti-cortactin antibody (Transduction Laboratories) at a dilution of 1:10,000, and it was developed by using horseradish peroxidase-conjugated anti-mouse immunoglobulin G (IgG) antibody (Zymed) at a dilution of 1:10,000 and an ECL Western blotting detection kit (Amersham Pharmacia). ..

    Staining:

    Article Title: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018
    Article Snippet: .. The cells were permeabilized with triton X-100, stained with palivizumab antibody followed by goat-anti-human IgG conjugated with HRP and coloured using chloronaphtol solution (Thermo Fisher Scientific). .. The neutralization titer was defined as the reciprocal of the highest antibody dilution producing 50% reduction in plaques (ED50), relative to virus-control wells without antibody.

    Western Blot:

    Article Title: DNA Topoisomerase II Is Involved in Regulation of Cyst Wall Protein Genes and Differentiation in Giardia lamblia
    Article Snippet: .. Western blots were probed with anti-V5-horseradish peroxidase (Invitrogen), anti-HA monoclonal antibody (1/5000 in blocking buffer; Sigma), anti-CWP1 (1/10000 in blocking buffer) , anti-Myb2 (1/5000 in blocking buffer) , anti-RAN (1/10000 in blocking buffer) , anti-Topo II (1/10000 in blocking buffer) (see below), or preimmune serum (1/5000 in blocking buffer), and detected with peroxidase-conjugated goat anti-mouse IgG (1/5000; Pierce) or peroxidase-conjugated goat anti-rabbit IgG (1/5000; Pierce) and enhanced chemiluminescence (GE Healthcare). .. Scanned images were analyzed by the ImageJ software (NIH, USA).

    Article Title: Cortactin Is Necessary for F-Actin Accumulation in Pedestal Structures Induced by Enteropathogenic Escherichia coli Infection
    Article Snippet: .. The membrane-bound protein was probed with the anti-cortactin antibody (Transduction Laboratories) at a dilution of 1:10,000, and it was developed by using horseradish peroxidase-conjugated anti-mouse immunoglobulin G (IgG) antibody (Zymed) at a dilution of 1:10,000 and an ECL Western blotting detection kit (Amersham Pharmacia). ..

    Blocking Assay:

    Article Title: DNA Topoisomerase II Is Involved in Regulation of Cyst Wall Protein Genes and Differentiation in Giardia lamblia
    Article Snippet: .. Western blots were probed with anti-V5-horseradish peroxidase (Invitrogen), anti-HA monoclonal antibody (1/5000 in blocking buffer; Sigma), anti-CWP1 (1/10000 in blocking buffer) , anti-Myb2 (1/5000 in blocking buffer) , anti-RAN (1/10000 in blocking buffer) , anti-Topo II (1/10000 in blocking buffer) (see below), or preimmune serum (1/5000 in blocking buffer), and detected with peroxidase-conjugated goat anti-mouse IgG (1/5000; Pierce) or peroxidase-conjugated goat anti-rabbit IgG (1/5000; Pierce) and enhanced chemiluminescence (GE Healthcare). .. Scanned images were analyzed by the ImageJ software (NIH, USA).

    Conjugation Assay:

    Article Title: Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth
    Article Snippet: .. HRP conjugation to seeMet antibodies was performed using EZ-link Plus Activated Peroxidase (Pierce/Thermo Scientific). .. HRP conjugated antibodies were used at 1 µg/mL on Western blotting.

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    Thermo Fisher horseradish peroxidase conjugated goat anti murine igg
    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with <t>mAb</t> IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse <t>IgG</t> and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.
    Horseradish Peroxidase Conjugated Goat Anti Murine Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti murine igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti murine igg - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher peroxidase conjugated secondary antibodies
    HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by <t>HRP-conjugated</t> <t>anti-mouse</t> <t>secondary</t> antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .
    Peroxidase Conjugated Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies/product/Thermo Fisher
    Average 92 stars, based on 815 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Thermo Fisher rabbit anti mouse horseradish peroxidase hrp conjugated secondary antibody
    c-di-GMP regulates TfoY protein levels. (A) Western blot of PAGE-separated protein isolated from V. cholerae parent strains carrying either the P 1234 -TL-FULL <t>gfp</t> reporter fusion or the G20U variant. Blots were hybridized with anti-GFP primary antibody and <t>HRP-conjugated</t> secondary antibody and detected using ECL. The blot shown is representative of multiple experiments. (B) Quantification of TfoY-GFP fusion protein present in the lanes depicted in panel A ( n = 3 or 4). Relative amounts of protein were quantified for each lane and normalized to the WT Vc2 riboswitch with the P tac -qrgB * group for each blot (set equal to 1 relative unit of protein). L, low; I, intermediate; H, high. Error bars for all graphs indicate the standard deviation. *, P
    Rabbit Anti Mouse Horseradish Peroxidase Hrp Conjugated Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse horseradish peroxidase hrp conjugated secondary antibody/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse horseradish peroxidase hrp conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin ?L?2 *

    doi: 10.1074/jbc.M112.361519

    Figure Lengend Snippet: Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Article Snippet: Murine anti-moesin mAb (clone 38/87) and horseradish peroxidase-conjugated goat anti-murine IgG were from Thermo Scientific (Fremont, CA).

    Techniques: Incubation, Derivative Assay, Flow Cytometry, Cytometry

    HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .

    Journal: bioRxiv

    Article Title: Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry

    doi: 10.1101/858472

    Figure Lengend Snippet: HSV-1 gC facilitates acid-induced conformational changes in the fusion protein gB. Extracellular preparations of HSV-1 gCR or ΔgC (∼ 10 7 genome copy numbers) were treated with pHs ranging from 7.3 to 5.0 and blotted directly to nitrocellulose. Blots were probed with the indicated gB MAbs H126, H1838, SS144, or H1359 at neutral pH followed by HRP-conjugated anti-mouse secondary antibody. The antibody name is shown at the left and the gB domain to which each MAb is directed is indicated in parenthesis. These are individual examples of experiments that were quantitated and averaged together with multiple similar independent determinations. Summarized quantitative results are depicted in Figure 6 .

    Article Snippet: After incubation with horseradish peroxidase-conjugated secondary antibodies, enhanced chemiluminescent substrate (Thermo Fisher Scientific) was added, and blots were exposed to X-ray film (Genesee Scientific).

    Techniques:

    c-di-GMP regulates TfoY protein levels. (A) Western blot of PAGE-separated protein isolated from V. cholerae parent strains carrying either the P 1234 -TL-FULL gfp reporter fusion or the G20U variant. Blots were hybridized with anti-GFP primary antibody and HRP-conjugated secondary antibody and detected using ECL. The blot shown is representative of multiple experiments. (B) Quantification of TfoY-GFP fusion protein present in the lanes depicted in panel A ( n = 3 or 4). Relative amounts of protein were quantified for each lane and normalized to the WT Vc2 riboswitch with the P tac -qrgB * group for each blot (set equal to 1 relative unit of protein). L, low; I, intermediate; H, high. Error bars for all graphs indicate the standard deviation. *, P

    Journal: Journal of Bacteriology

    Article Title: Cyclic di-GMP Regulates TfoY in Vibrio cholerae To Control Motility by both Transcriptional and Posttranscriptional Mechanisms

    doi: 10.1128/JB.00578-17

    Figure Lengend Snippet: c-di-GMP regulates TfoY protein levels. (A) Western blot of PAGE-separated protein isolated from V. cholerae parent strains carrying either the P 1234 -TL-FULL gfp reporter fusion or the G20U variant. Blots were hybridized with anti-GFP primary antibody and HRP-conjugated secondary antibody and detected using ECL. The blot shown is representative of multiple experiments. (B) Quantification of TfoY-GFP fusion protein present in the lanes depicted in panel A ( n = 3 or 4). Relative amounts of protein were quantified for each lane and normalized to the WT Vc2 riboswitch with the P tac -qrgB * group for each blot (set equal to 1 relative unit of protein). L, low; I, intermediate; H, high. Error bars for all graphs indicate the standard deviation. *, P

    Article Snippet: Blots were blocked with 5% nonfat milk in Tris-buffered saline with 0.1% Tween 20 (TBS-T) and hybridized with a mouse anti-GFP primary antibody and a rabbit anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (both Thermo Fisher Scientific).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, Isolation, Variant Assay, Standard Deviation