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Santa Cruz Biotechnology peroxidase conjugated secondary antibodies
Peroxidase Conjugated Secondary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase conjugated secondary antibodies/product/Santa Cruz Biotechnology
Average 92 stars, based on 655 article reviews
Price from $9.99 to $1999.99
peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Western Blot:

Article Title: Encapsulated Cell Technology-Based Delivery of a Complement Inhibitor Reduces Choroidal Neovascularization in a Mouse Model
Article Snippet: .. Proteins were visualized with horseradish peroxidase-conjugated secondary antibodies (anti-mouse IgG and IgM; Santa Cruz Biotechnology) followed by incubation with Clarity™ Western ECL Blotting Substrate and chemiluminescent detection. .. Immunohistochemistry Eyecups were collected as previously described, with the lens and anterior chamber removed, fixed overnight with 4% paraformaldehyde (PFA), washed in PBS, embedded in Neg-50 cutting medium (Richard-Allen Scientific; Thermo Fisher Scientific) and sectioned using a cryostat (14-μm sections).

Incubation:

Article Title: Agonist-induced phosphorylation bar code and differential post-activation signaling of the delta opioid receptor revealed by phosphosite-specific antibodies
Article Snippet: .. Cells were washed several times with PBS and incubated with a peroxidase-conjugated secondary antibody (Santa Cruz; Heidelberg, Germany). .. After additional washing steps, the HRP-substrate ABTS was added and optical density was measured at 405 nm using an iMark™ Microplate Absorbance Reader (BioRad, Munich, Germany).

Article Title: Encapsulated Cell Technology-Based Delivery of a Complement Inhibitor Reduces Choroidal Neovascularization in a Mouse Model
Article Snippet: .. Proteins were visualized with horseradish peroxidase-conjugated secondary antibodies (anti-mouse IgG and IgM; Santa Cruz Biotechnology) followed by incubation with Clarity™ Western ECL Blotting Substrate and chemiluminescent detection. .. Immunohistochemistry Eyecups were collected as previously described, with the lens and anterior chamber removed, fixed overnight with 4% paraformaldehyde (PFA), washed in PBS, embedded in Neg-50 cutting medium (Richard-Allen Scientific; Thermo Fisher Scientific) and sectioned using a cryostat (14-μm sections).

Article Title: Insulin-Like Growth Factor Binding Proteins 3 and 5 Are Overexpressed in Idiopathic Pulmonary Fibrosis and Contribute to Extracellular Matrix Deposition
Article Snippet: .. Signals were detected after incubation with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology, Inc.) and chemiluminescence (Perkin Elmer Life Sciences, Inc., Boston, MA). ..

other:

Article Title: 6-bromo-indirubin-3′-oxime (6BIO), a Glycogen synthase kinase-3β inhibitor, activates cytoprotective cellular modules and suppresses cellular senescence-mediated biomolecular damage in human fibroblasts
Article Snippet: Antibodies used Primary antibodies against Nrf2 (sc-722), proteasome α7 subunit (sc-100456), proteasome β5 subunit (sc-55009), Beclin1 (sc-11427), Gsk-3β (sc-9166), p53 (sc-47698), p21WAF1/CIP1 (sc-6246), Actin (sc-1615) and GAPDH (sc-25778), as well as the horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology.

Article Title: A Pilot Study to Assess Effects of Long-Term Inhalation of Airborne Particulate Matter on Early Alzheimer-Like Changes in the Mouse Brain
Article Snippet: All horseradish peroxidase conjugated secondary antibodies donkey anti-rabbit IgG (sc-2317), bovine anti-goat IgG (sc-2350), goat anti-mouse IgM (sc-2064), bovine anti- mouse IgG (sc-2371) were from Santa Cruz Biotechnology Inc. (Dallas, TX).

Immunoprecipitation:

Article Title: Role of Cortactin in Dynamic Actin Remodeling Events in Gonadotrope Cells
Article Snippet: .. All horseradish peroxidase-conjugated secondary antibodies, anti–phospho-ERK, and the protein A/G immunoprecipitation beads were purchased from Santa Cruz Biotechnology Inc.. .. The anti-cortactin, anti–phospho-cortactin Y421 antibody, anti–phospho-Akt Y473 , anti–phospho-Src Y416 , ERK1/2, and β-tubulin were purchased from Cell Signaling Technology.

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  • 88
    Santa Cruz Biotechnology hrp conjugated goat antirabbit igg
    SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat <t>antirabbit</t> <t>IgG</t> against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P
    Hrp Conjugated Goat Antirabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat antirabbit igg/product/Santa Cruz Biotechnology
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat antirabbit igg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Santa Cruz Biotechnology
    Average 92 stars, based on 434 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology hrp conjugated anti mouse igg antibody
    Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and <t>HRP-conjugated</t> anti-mouse <t>IgG</t> antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.
    Hrp Conjugated Anti Mouse Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti mouse igg antibody/product/Santa Cruz Biotechnology
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated anti mouse igg antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology goat horseradish peroxidase hrp conjugated secondary anti mouse antibody
    <t>BtaE</t> localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse <t>HRP-conjugated</t> secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.
    Goat Horseradish Peroxidase Hrp Conjugated Secondary Anti Mouse Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat horseradish peroxidase hrp conjugated secondary anti mouse antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat horseradish peroxidase hrp conjugated secondary anti mouse antibody - by Bioz Stars, 2020-07
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    Image Search Results


    SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat antirabbit IgG against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P

    Journal: Clinical and Developmental Immunology

    Article Title: Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE2 and Th2 Cytokines

    doi: 10.1155/2011/917015

    Figure Lengend Snippet: SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat antirabbit IgG against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P

    Article Snippet: The secondary antibody, HRP-conjugated goat antirabbit IgG (Santa Cruz Biotechnology, Inc.), was diluted 1 : 1000.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Western Blot, Incubation, Microscopy, Purification, Recombinant, Positive Control, Negative Control

    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Journal: Proteome Science

    Article Title: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus

    doi: 10.1186/1477-5956-8-28

    Figure Lengend Snippet: Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Article Snippet: The blots were then washed four times with PBS containing 0.1% Tween-20, and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) 1 hour at room temperature.

    Techniques: Western Blot, Infection, SDS Page, Staining

    Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and HRP-conjugated anti-mouse IgG antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.

    Journal: Virology Journal

    Article Title: Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies

    doi: 10.1186/1743-422X-11-96

    Figure Lengend Snippet: Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and HRP-conjugated anti-mouse IgG antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.

    Article Snippet: For immunodetection of the His fusion proteins, the primary antibody used was His probe (H-3) mouse monoclonal IgG (1 : 500; Santa Cruz Biotechnology, USA) and the secondary antibody was HRP-conjugated anti-mouse IgG antibody (1 : 2000; Santa Cruz Biotechnology, USA).

    Techniques: Expressing, Recombinant, Staining, SDS Page, Incubation, Marker, Western Blot, Blocking Assay

    BtaE localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse HRP-conjugated secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.

    Journal: Infection and Immunity

    Article Title: BtaE, an Adhesin That Belongs to the Trimeric Autotransporter Family, Is Required for Full Virulence and Defines a Specific Adhesive Pole of Brucella suis

    doi: 10.1128/IAI.01241-12

    Figure Lengend Snippet: BtaE localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse HRP-conjugated secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.

    Article Snippet: Blots were probed with polyclonal mouse anti-BtaE serum (1:8,000) and a goat horseradish peroxidase (HRP)-conjugated secondary anti-mouse antibody (1:30,000; Santa Cruz) and then revealed using ECL Plus (Amersham).

    Techniques: Western Blot, SDS Page, Incubation, Immunofluorescence, Labeling, Microscopy