peroxidase conjugated secondary antibodies  (Roche)

 
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    Structured Review

    Roche peroxidase conjugated secondary antibodies
    Peroxidase Conjugated Secondary Antibodies, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibodies/product/Roche
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars

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    Western Blot:

    Article Title: The S100A4 Transcriptional Inhibitor Niclosamide Reduces Pro-Inflammatory and Migratory Phenotypes of Microglia: Implications for Amyotrophic Lateral Sclerosis
    Article Snippet: .. After rinsing with Tris-buffered saline solution with 0.1% Tween-20 (TBS-T), membranes were incubated for 1 h with the appropriate peroxidase-conjugated secondary antibody, then washed and developed using the ECL chemiluminescence detection system (Roche) or Advance Western blot detection kit (Amersham Biosciences, Buckinghamshire, UK). ..

    Incubation:

    Article Title: Down-Regulation of the Histone Methyltransferase EZH2 Contributes to the Epigenetic Programming of Decidualizing Human Endometrial Stromal Cells
    Article Snippet: .. The proteins were visualized by incubation with horseradish peroxidase-conjugated secondary antibodies (Roche) and the chemiluminescence detected using the ECL+ kit (GE Healthcare, Indianapolis, IN). .. RNA was extracted using STAT-60 (AMS Biotech, Abingdon, UK) and following manufacturer's instructions. cDNA was synthesized using the SuperScript First-Strand Synthesis for RT-PCR (Invitrogen, Carlsbad, CA) with oligo-dT primers (Invitrogen) after having treated the extracted RNA with amplification grade deoxyribonuclease I (Invitrogen).

    Article Title: The Roles of ATF3, an Adaptive-Response Gene, in High-Fat-Diet-Induced Diabetes and Pancreatic ?-Cell Dysfunction
    Article Snippet: .. Membranes were then washed three times with TBST and incubated with a horseradish peroxidase-conjugated secondary antibody for 1 h. Membranes were again washed three times before chemiluminescent development (Roche, Indianapolis, IN). .. Immunohistochemistry was performed on paraffin-embedded tissue samples after xyline deparaffin incubation and rehydration through ethanol into water.

    Article Title: Induction of 11β-HSD 1 and Activation of Distinct Mineralocorticoid Receptor- and Glucocorticoid Receptor-Dependent Gene Networks in Decidualizing Human Endometrial Stromal Cells
    Article Snippet: .. After incubation with horseradish peroxidase-conjugated secondary antibodies, 1:2500, (Roche Diagnostics, Mannheim, Germany), chemiluminescence was visualized using the ECL+ chemiluminescence detection kit (Amersham, Little Chalfont, UK). ..

    Article Title: The S100A4 Transcriptional Inhibitor Niclosamide Reduces Pro-Inflammatory and Migratory Phenotypes of Microglia: Implications for Amyotrophic Lateral Sclerosis
    Article Snippet: .. After rinsing with Tris-buffered saline solution with 0.1% Tween-20 (TBS-T), membranes were incubated for 1 h with the appropriate peroxidase-conjugated secondary antibody, then washed and developed using the ECL chemiluminescence detection system (Roche) or Advance Western blot detection kit (Amersham Biosciences, Buckinghamshire, UK). ..

    Article Title: Tissue-specific deregulation of selected HDACs characterizes ALS progression in mouse models: pharmacological characterization of SIRT1 and SIRT2 pathways
    Article Snippet: .. After washing with 0.1% Tween in Tris-buffered saline, the membrane was incubated with peroxidase-conjugated secondary antibody for 1 h, and then washed and developed using the ECL chemiluminescent detection system (Roche). .. Densitometric analyses were performed using ImageJ software program (National Institutes of Health, Bethesda, MD, USA; http://imagej.nih.gov/ij/ ) and normalized against the signal obtained by reprobing the membranes with mouse anti-β- actin or anti-tubulin.

    Article Title: Stable Inhibition of Specific Estrogen Receptor ? (ER?) Phosphorylation Confers Increased Growth, Migration/Invasion, and Disruption of Estradiol Signaling in MCF-7 Breast Cancer Cells
    Article Snippet: .. Cells were then incubated with a peroxidase-conjugated secondary antibody directed against BrdU, anti-BrdU (Roche), for 1 h. Cells were washed in kit-supplied 1× washing solution. .. Cells were incubated with kit-supplied substrate 3,3′,5,5′-tetramethylbenzidine for 1 h at RT.

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    Roche secondary horseradish peroxidase conjugated abs
    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the <t>anti-p185</t> her2/neu Ab 1E1. The <t>ds-oligo-conjugated</t> mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human <t>IgG-horseradish</t> <t>peroxidase</t> (Zymed) was used as the <t>secondary</t> Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.
    Secondary Horseradish Peroxidase Conjugated Abs, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary horseradish peroxidase conjugated abs/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary horseradish peroxidase conjugated abs - by Bioz Stars, 2020-07
    88/100 stars
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    88
    Roche antibodies hrp conjugated goat anti rabbit igg
    The effect of Hyp on Bax, Bcl-2, Cyto C, caspase-3, caspase-9 expression of RVECs from diabetic rats (n=4). (A) Photograph of protein expression by western blot. (B) Statistical analysis of protein expression by western blot. RVECs derived from each group of rats were collected and lysed in 100 μl RIPA buffer containing cOmplete™ Protease Inhibitor Cocktail. The cell lysates were centrifuged and the supernatant was kept. The protein concentration was determined by BCA method. 30 μg of protein was applied on SDS-PAGE. The protein gels were transferred onto PVDF membranes. Primary antibody anti-Bax, anti-Bcl-2, anti-Cyto C, anti–caspase-3, anti–caspase-9 (1: 500, overnight at 4 ℃), secondary antibodies <t>HRP-conjugated</t> goat anti-rabbit <t>IgG</t> (1:2000) or HRP-goat anti-mouse IgG (1:5000). Protein was detected using ECL system and normalized by β-actin. ## P
    Antibodies Hrp Conjugated Goat Anti Rabbit Igg, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies hrp conjugated goat anti rabbit igg/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies hrp conjugated goat anti rabbit igg - by Bioz Stars, 2020-07
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    85
    Roche secondary peroxidase conjugated immunoglobulin g immune complexes
    CDC50A is expressed in detergent-resistant membranes of hepatocytes (parenchymal) and liver sinusoidal endothelial cells. Cell fractions were isolated as described in Materials and Methods. (A) FACS scan profiles demonstrate an enrichment of CD146-positive cells in the eluate compared to the nonparenchymal cell fraction. Cells were labeled with FITC-conjugated anti-Cd146 (black curve) and a rat FITC-conjugated <t>IgG2a</t> K isotype control (gray curve). (B) Western analyses of cell fractions analyzed for CDC50A, glutamine synthetase, ATP1A1, and ATP8B1. (C) Western analyses of mixed (m), canalicular (c), and basolateral (b) liver plasma membranes (LPM) for CDC50A, ABCC2, SLC10A1, and ATP8B1 expression. (D) Analysis of alkaline phosphatase activity and CDC50A, caveolin-1, and ATP8B1 expression in TX-100 resistant membranes. Blots were incubated with CDC50A antiserum. Representative experiments of at least three independent experiments are shown.
    Secondary Peroxidase Conjugated Immunoglobulin G Immune Complexes, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary peroxidase conjugated immunoglobulin g immune complexes/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary peroxidase conjugated immunoglobulin g immune complexes - by Bioz Stars, 2020-07
    85/100 stars
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    88
    Roche horseradish peroxidase conjugated secondary igg
    CDC50A is expressed in detergent-resistant membranes of hepatocytes (parenchymal) and liver sinusoidal endothelial cells. Cell fractions were isolated as described in Materials and Methods. (A) FACS scan profiles demonstrate an enrichment of CD146-positive cells in the eluate compared to the nonparenchymal cell fraction. Cells were labeled with FITC-conjugated anti-Cd146 (black curve) and a rat FITC-conjugated <t>IgG2a</t> K isotype control (gray curve). (B) Western analyses of cell fractions analyzed for CDC50A, glutamine synthetase, ATP1A1, and ATP8B1. (C) Western analyses of mixed (m), canalicular (c), and basolateral (b) liver plasma membranes (LPM) for CDC50A, ABCC2, SLC10A1, and ATP8B1 expression. (D) Analysis of alkaline phosphatase activity and CDC50A, caveolin-1, and ATP8B1 expression in TX-100 resistant membranes. Blots were incubated with CDC50A antiserum. Representative experiments of at least three independent experiments are shown.
    Horseradish Peroxidase Conjugated Secondary Igg, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary igg/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary igg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the anti-p185 her2/neu Ab 1E1. The ds-oligo-conjugated mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human IgG-horseradish peroxidase (Zymed) was used as the secondary Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution

    doi: 10.1073/pnas.101124598

    Figure Lengend Snippet: A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the anti-p185 her2/neu Ab 1E1. The ds-oligo-conjugated mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human IgG-horseradish peroxidase (Zymed) was used as the secondary Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.

    Article Snippet: All polyclonal sera and secondary horseradish peroxidase-conjugated Abs (Roche Molecular Biochemicals) were used at a 1:5,000 dilution.

    Techniques: Amplification, Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Western Blot, Polyacrylamide Gel Electrophoresis

    The effect of Hyp on Bax, Bcl-2, Cyto C, caspase-3, caspase-9 expression of RVECs from diabetic rats (n=4). (A) Photograph of protein expression by western blot. (B) Statistical analysis of protein expression by western blot. RVECs derived from each group of rats were collected and lysed in 100 μl RIPA buffer containing cOmplete™ Protease Inhibitor Cocktail. The cell lysates were centrifuged and the supernatant was kept. The protein concentration was determined by BCA method. 30 μg of protein was applied on SDS-PAGE. The protein gels were transferred onto PVDF membranes. Primary antibody anti-Bax, anti-Bcl-2, anti-Cyto C, anti–caspase-3, anti–caspase-9 (1: 500, overnight at 4 ℃), secondary antibodies HRP-conjugated goat anti-rabbit IgG (1:2000) or HRP-goat anti-mouse IgG (1:5000). Protein was detected using ECL system and normalized by β-actin. ## P

    Journal: Frontiers in Pharmacology

    Article Title: Hyperoside Ameliorates Diabetic Retinopathy via Anti-Oxidation, Inhibiting Cell Damage and Apoptosis Induced by High Glucose

    doi: 10.3389/fphar.2020.00797

    Figure Lengend Snippet: The effect of Hyp on Bax, Bcl-2, Cyto C, caspase-3, caspase-9 expression of RVECs from diabetic rats (n=4). (A) Photograph of protein expression by western blot. (B) Statistical analysis of protein expression by western blot. RVECs derived from each group of rats were collected and lysed in 100 μl RIPA buffer containing cOmplete™ Protease Inhibitor Cocktail. The cell lysates were centrifuged and the supernatant was kept. The protein concentration was determined by BCA method. 30 μg of protein was applied on SDS-PAGE. The protein gels were transferred onto PVDF membranes. Primary antibody anti-Bax, anti-Bcl-2, anti-Cyto C, anti–caspase-3, anti–caspase-9 (1: 500, overnight at 4 ℃), secondary antibodies HRP-conjugated goat anti-rabbit IgG (1:2000) or HRP-goat anti-mouse IgG (1:5000). Protein was detected using ECL system and normalized by β-actin. ## P

    Article Snippet: The membranes were incubated with primary antibody anti-Bax (1:500), anti-Bcl-2 (1:500), anti-Cyto C (1:500), anti–caspase-3 (1:500), anti–caspase-9 (1:500) overnight at 4°C, and then incubated with secondary antibodies HRP-conjugated goat anti-rabbit IgG (1:2000) or HRP-conjugated goat anti-mouse IgG (1:5000) at room temperature for 1 h. Protein was detected using ECL system (Roche) and normalized by β-actin.

    Techniques: Expressing, Western Blot, Derivative Assay, Protease Inhibitor, Protein Concentration, SDS Page

    CDC50A is expressed in detergent-resistant membranes of hepatocytes (parenchymal) and liver sinusoidal endothelial cells. Cell fractions were isolated as described in Materials and Methods. (A) FACS scan profiles demonstrate an enrichment of CD146-positive cells in the eluate compared to the nonparenchymal cell fraction. Cells were labeled with FITC-conjugated anti-Cd146 (black curve) and a rat FITC-conjugated IgG2a K isotype control (gray curve). (B) Western analyses of cell fractions analyzed for CDC50A, glutamine synthetase, ATP1A1, and ATP8B1. (C) Western analyses of mixed (m), canalicular (c), and basolateral (b) liver plasma membranes (LPM) for CDC50A, ABCC2, SLC10A1, and ATP8B1 expression. (D) Analysis of alkaline phosphatase activity and CDC50A, caveolin-1, and ATP8B1 expression in TX-100 resistant membranes. Blots were incubated with CDC50A antiserum. Representative experiments of at least three independent experiments are shown.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Cellular Localization and Biochemical Analysis of Mammalian CDC50A, a Glycosylated ?-subunit for P4 ATPases

    doi: 10.1369/0022155411435705

    Figure Lengend Snippet: CDC50A is expressed in detergent-resistant membranes of hepatocytes (parenchymal) and liver sinusoidal endothelial cells. Cell fractions were isolated as described in Materials and Methods. (A) FACS scan profiles demonstrate an enrichment of CD146-positive cells in the eluate compared to the nonparenchymal cell fraction. Cells were labeled with FITC-conjugated anti-Cd146 (black curve) and a rat FITC-conjugated IgG2a K isotype control (gray curve). (B) Western analyses of cell fractions analyzed for CDC50A, glutamine synthetase, ATP1A1, and ATP8B1. (C) Western analyses of mixed (m), canalicular (c), and basolateral (b) liver plasma membranes (LPM) for CDC50A, ABCC2, SLC10A1, and ATP8B1 expression. (D) Analysis of alkaline phosphatase activity and CDC50A, caveolin-1, and ATP8B1 expression in TX-100 resistant membranes. Blots were incubated with CDC50A antiserum. Representative experiments of at least three independent experiments are shown.

    Article Snippet: B. Koenderink) , ATP8B1 ( ) and caveolin (Santa Cruz Biotechnology, Santa Cruz, CA), and secondary peroxidase-conjugated immunoglobulin G. Immune complexes were visualized with Lumi-Light Western blotting substrate (Roche).

    Techniques: Isolation, FACS, Labeling, Western Blot, Expressing, Activity Assay, Incubation