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Boehringer Mannheim peroxidase conjugated goat anti rabbit
Peroxidase Conjugated Goat Anti Rabbit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peroxidase conjugated goat anti rabbit/product/Boehringer Mannheim
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
peroxidase conjugated goat anti rabbit - by Bioz Stars, 2020-08
92/100 stars

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Incubation:

Article Title: Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
Article Snippet: .. Membranes were incubated with primary antibodies, affinity-purified α-At-Rac (diluted 1:200) or a monoclonal mouse anti-actin antibody ( Boehringer Mannheim ; diluted 1:2,500), and secondary antibodies, peroxidase-conjugated goat anti–rabbit or goat anti–mouse antibodies ( Boehringer Mannheim ; diluted 1:5,000), for 1 h at room temperature in Tris-buffered saline (TBS; 25 mM Tris-HCl at pH 7.4; 137 mM NaCl, 5 mM KCl) supplemented with 4% BSA (A-3059; Sigma Chemical Co. ). .. After incubation with primary and secondary antibodies, membranes were washed twice for 10 min with TBS containing 4% BSA and 0.02% Tween 20.

Affinity Purification:

Article Title: Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
Article Snippet: .. Membranes were incubated with primary antibodies, affinity-purified α-At-Rac (diluted 1:200) or a monoclonal mouse anti-actin antibody ( Boehringer Mannheim ; diluted 1:2,500), and secondary antibodies, peroxidase-conjugated goat anti–rabbit or goat anti–mouse antibodies ( Boehringer Mannheim ; diluted 1:5,000), for 1 h at room temperature in Tris-buffered saline (TBS; 25 mM Tris-HCl at pH 7.4; 137 mM NaCl, 5 mM KCl) supplemented with 4% BSA (A-3059; Sigma Chemical Co. ). .. After incubation with primary and secondary antibodies, membranes were washed twice for 10 min with TBS containing 4% BSA and 0.02% Tween 20.

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  • 92
    Boehringer Mannheim peroxidase conjugated goat anti rabbit igg
    Effect of CV-N on binding of gp120 and <t>anti-CD4</t> antibodies to cell-associated CD4. (A) Binding of mock-treated (lane 3) or CV-N-treated (lanes 4 to 6) JR-FL gp120 to CD4-expressing (vCB-3-infected) cells was determined by Western blotting of whole cell lysates using polyclonal anti-gp120 antibody for detection. For lane 9, gp120 was preincubated with excess of sCD4. As negative control, CD4-negative (WR-infected) cells were incubated without (lane 7) or with (lane 8) gp120. The indicated CV-N concentrations represent those in the final incubation mixtures. Purified JR-FL gp120 (lane 1) was run as positive standard for immunodetection. mCD4, membrane-associated CD4. (B) Effect of CV-N on binding of anti-CD4 polyclonal antiserum to CD4-expressing (lanes 2 to 4) or CD4-negative cells (lanes 5 and 6) was determined by Western blotting of whole cell lysates using horseradish peroxidase-conjugated goat anti-rabbit <t>IgG.</t> The indicated CV-N concentrations represent those in the final incubation mixture. In lane 1, diluted antiserum was run as a positive standard. Migration of IgG heavy chain (hc) is indicated.
    Peroxidase Conjugated Goat Anti Rabbit Igg, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti rabbit igg/product/Boehringer Mannheim
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    80
    Boehringer Mannheim horseradish peroxidase conjugated goat anti rabbit serum
    Western blot of selected env clones. Selected env clones from the HIV-1 RC-br and HIV-1 DS-br isolates were resolved by sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis transferred to a polyvinylidene difluoride membrane, and probed with a polyclonal <t>rabbit</t> <t>anti-gp120</t> <t>serum</t> followed by <t>peroxidase-conjugated</t> <t>goat</t> anti-rabbit serum, and the bound antibody was detected by enhanced chemiluminescence (Amersham). Recombinant gp120 prepared in a baculovirus system was used as a positive control (+ lane); the slight difference in mobility may be due to differences in glycosylation. “None” represents a lysate from cells transfected with pNL4-3-LucR + E − only. All of the env clones analyzed resulted in proteins that were processed into gp120.
    Horseradish Peroxidase Conjugated Goat Anti Rabbit Serum, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti rabbit serum/product/Boehringer Mannheim
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti rabbit serum - by Bioz Stars, 2020-08
    80/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim peroxidase conjugated goat anti rabbit antibody
    Reactivity of CW MAbs and R140 with HveA(120t) peptides. A diagram of HveA is shown. The four CRP elements comprising the HveA ectodomain are represented by ovals and are numbered 1 to 4. Eight overlapping synthetic peptides (seven 15-mers and one 12-mer [shown as solid bars with the HveA residue numbers indicated]) mimicking residues within the first and second CRP elements of HveA were directly coupled to a cellulose sheet. Peptide 4 spans the junction between CRP1 and CRP2 (indicated by an arrow). Individual strips containing the complete set of peptides were incubated with the indicated CW MAb or the <t>rabbit</t> polyclonal antiserum R140. Reactive peptide spots were then visualized by ECL after incubation with the appropriate horseradish <t>peroxidase-conjugated</t> secondary <t>antibody.</t> TMR, transmembrane region.
    Peroxidase Conjugated Goat Anti Rabbit Antibody, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti rabbit antibody/product/Boehringer Mannheim
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti rabbit antibody - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    Effect of CV-N on binding of gp120 and anti-CD4 antibodies to cell-associated CD4. (A) Binding of mock-treated (lane 3) or CV-N-treated (lanes 4 to 6) JR-FL gp120 to CD4-expressing (vCB-3-infected) cells was determined by Western blotting of whole cell lysates using polyclonal anti-gp120 antibody for detection. For lane 9, gp120 was preincubated with excess of sCD4. As negative control, CD4-negative (WR-infected) cells were incubated without (lane 7) or with (lane 8) gp120. The indicated CV-N concentrations represent those in the final incubation mixtures. Purified JR-FL gp120 (lane 1) was run as positive standard for immunodetection. mCD4, membrane-associated CD4. (B) Effect of CV-N on binding of anti-CD4 polyclonal antiserum to CD4-expressing (lanes 2 to 4) or CD4-negative cells (lanes 5 and 6) was determined by Western blotting of whole cell lysates using horseradish peroxidase-conjugated goat anti-rabbit IgG. The indicated CV-N concentrations represent those in the final incubation mixture. In lane 1, diluted antiserum was run as a positive standard. Migration of IgG heavy chain (hc) is indicated.

    Journal: Journal of Virology

    Article Title: Multiple Antiviral Activities of Cyanovirin-N: Blocking of Human Immunodeficiency Virus Type 1 gp120 Interaction with CD4 and Coreceptor and Inhibition of Diverse Enveloped Viruses

    doi:

    Figure Lengend Snippet: Effect of CV-N on binding of gp120 and anti-CD4 antibodies to cell-associated CD4. (A) Binding of mock-treated (lane 3) or CV-N-treated (lanes 4 to 6) JR-FL gp120 to CD4-expressing (vCB-3-infected) cells was determined by Western blotting of whole cell lysates using polyclonal anti-gp120 antibody for detection. For lane 9, gp120 was preincubated with excess of sCD4. As negative control, CD4-negative (WR-infected) cells were incubated without (lane 7) or with (lane 8) gp120. The indicated CV-N concentrations represent those in the final incubation mixtures. Purified JR-FL gp120 (lane 1) was run as positive standard for immunodetection. mCD4, membrane-associated CD4. (B) Effect of CV-N on binding of anti-CD4 polyclonal antiserum to CD4-expressing (lanes 2 to 4) or CD4-negative cells (lanes 5 and 6) was determined by Western blotting of whole cell lysates using horseradish peroxidase-conjugated goat anti-rabbit IgG. The indicated CV-N concentrations represent those in the final incubation mixture. In lane 1, diluted antiserum was run as a positive standard. Migration of IgG heavy chain (hc) is indicated.

    Article Snippet: Samples were resolved by polyacrylamide gel electrophoresis, and the anti-CD4 IgG heavy chain was detected by Western blotting with horseradish peroxidase-conjugated goat anti-rabbit IgG and SuperSignal chemiluminiscent substrates.

    Techniques: Binding Assay, Expressing, Infection, Western Blot, Negative Control, Incubation, Purification, Immunodetection, Migration

    Western blot of selected env clones. Selected env clones from the HIV-1 RC-br and HIV-1 DS-br isolates were resolved by sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis transferred to a polyvinylidene difluoride membrane, and probed with a polyclonal rabbit anti-gp120 serum followed by peroxidase-conjugated goat anti-rabbit serum, and the bound antibody was detected by enhanced chemiluminescence (Amersham). Recombinant gp120 prepared in a baculovirus system was used as a positive control (+ lane); the slight difference in mobility may be due to differences in glycosylation. “None” represents a lysate from cells transfected with pNL4-3-LucR + E − only. All of the env clones analyzed resulted in proteins that were processed into gp120.

    Journal: Journal of Virology

    Article Title: Chemokine Receptor Utilization by Human Immunodeficiency Virus Type 1 Isolates That Replicate in Microglia

    doi:

    Figure Lengend Snippet: Western blot of selected env clones. Selected env clones from the HIV-1 RC-br and HIV-1 DS-br isolates were resolved by sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis transferred to a polyvinylidene difluoride membrane, and probed with a polyclonal rabbit anti-gp120 serum followed by peroxidase-conjugated goat anti-rabbit serum, and the bound antibody was detected by enhanced chemiluminescence (Amersham). Recombinant gp120 prepared in a baculovirus system was used as a positive control (+ lane); the slight difference in mobility may be due to differences in glycosylation. “None” represents a lysate from cells transfected with pNL4-3-LucR + E − only. All of the env clones analyzed resulted in proteins that were processed into gp120.

    Article Snippet: The proteins were transferred to a polyvinylidene difluoride membrane by using a semidry apparatus, then probed with a rabbit polyclonal anti-gp120 serum (R2143; a gift of P. Earl, NIAID) followed by horseradish peroxidase-conjugated goat anti-rabbit serum (Boehringer Mannheim), and visualized by chemiluminescence (Amersham).

    Techniques: Western Blot, Clone Assay, Polyacrylamide Gel Electrophoresis, Recombinant, Positive Control, Transfection

    Reactivity of CW MAbs and R140 with HveA(120t) peptides. A diagram of HveA is shown. The four CRP elements comprising the HveA ectodomain are represented by ovals and are numbered 1 to 4. Eight overlapping synthetic peptides (seven 15-mers and one 12-mer [shown as solid bars with the HveA residue numbers indicated]) mimicking residues within the first and second CRP elements of HveA were directly coupled to a cellulose sheet. Peptide 4 spans the junction between CRP1 and CRP2 (indicated by an arrow). Individual strips containing the complete set of peptides were incubated with the indicated CW MAb or the rabbit polyclonal antiserum R140. Reactive peptide spots were then visualized by ECL after incubation with the appropriate horseradish peroxidase-conjugated secondary antibody. TMR, transmembrane region.

    Journal: Journal of Virology

    Article Title: Localization of the gD-Binding Region of the Human Herpes Simplex Virus Receptor, HveA

    doi: 10.1128/JVI.75.1.171-180.2001

    Figure Lengend Snippet: Reactivity of CW MAbs and R140 with HveA(120t) peptides. A diagram of HveA is shown. The four CRP elements comprising the HveA ectodomain are represented by ovals and are numbered 1 to 4. Eight overlapping synthetic peptides (seven 15-mers and one 12-mer [shown as solid bars with the HveA residue numbers indicated]) mimicking residues within the first and second CRP elements of HveA were directly coupled to a cellulose sheet. Peptide 4 spans the junction between CRP1 and CRP2 (indicated by an arrow). Individual strips containing the complete set of peptides were incubated with the indicated CW MAb or the rabbit polyclonal antiserum R140. Reactive peptide spots were then visualized by ECL after incubation with the appropriate horseradish peroxidase-conjugated secondary antibody. TMR, transmembrane region.

    Article Snippet: Plates were then washed three times with PBST and incubated for 30 min in horseradish peroxidase-conjugated goat anti-rabbit antibody (Boehringer Mannheim) diluted 1/1,000 in blocking solution.

    Techniques: Incubation