peroxidase conjugated goat anti mouse  (Jackson Immuno)

 
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  • 97
    Name:
    Peroxidase AffiniPure Goat Anti Mouse IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    115-035-003
    Price:
    115.0
    Category:
    Whole IgG Affinity Purified Antibodies
    Conjugate:
    Horseradish Peroxidase
    Size:
    2 0 ml
    Format:
    Whole IgG
    Host:
    Goat
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    Structured Review

    Jackson Immuno peroxidase conjugated goat anti mouse
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/peroxidase conjugated goat anti mouse/product/Jackson Immuno
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti mouse - by Bioz Stars, 2021-06
    97/100 stars

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    Related Articles

    other:

    Article Title: UNC-45A is required for neurite extension via controlling NMII activation
    Article Snippet: Chemicals, antibodies, and plasmids We used blebbistatin (Sigma-Aldrich), 5-(4,6-dichlorotriazinyl)aminofluorescein (ThermoFisher Scientific), anti–UNC-45A (Enzo Life Sciences), anti-NMIIA (BioLegend), anti–p-NMII LC (Ser-20; Abcam), anti-NMIIB (Santa Cruz Biotechnology), anti-actin (Sigma-Aldrich), Texas Red–goat anti-mouse immunoglobulin G (IgG), Texas Red–goat anti-rabbit IgG, fluorescein isothiocyanate (FITC)–donkey and anti-mouse IgG, peroxidase–goat anti-mouse IgG, peroxidase–goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories), Neurobasal medium (Invitrogen), B-27 (Invitrogen), FGF2 (Invitrogen), mouse PDGHbb (Invitrogen), pRRLsinPPT, pMDLg/pRRE, pMD2.G, and pRSV-Rev plasmids (gifts from Alyson E. Fournier, Montreal Neurological Institute, Montreal, Canada), and Lenti-X concentrator (Clontech).

    Article Title: Generation and Characterization of Function-blocking Anti-ectodysplasin A (EDA) Monoclonal Antibodies That Induce Ectodermal Dysplasia *
    Article Snippet: Plates were revealed with serial dilutions of anti-EDA antibodies, followed by peroxidase-coupled goat anti-mouse IgG (1/8000).

    Construct:

    Article Title: Molecular and Therapeutic Characterization of Anti-ectodysplasin A Receptor (EDAR) Agonist Monoclonal Antibodies *
    Article Snippet: For epitope mapping, ELISA plates were coated with an F(ab′)2 fragment of a goat anti-human Ig (Jackson ImmunoResearch) to capture various EDAR-Fc constructs or a control receptor (B cell maturation antigen-Fc) in cell supernatants. .. EDAR-Fc constructs and B cell maturation antigen-Fc were revealed either with peroxidase-coupled donkey anti-human (H+L) (Jackson ImmunoResearch) or with anti-EDAR antibodies at 1 μg/ml followed by peroxidase-coupled goat anti-mouse IgG (Jackson ImmunoResearch) or with FLAG-EDA1 or FLAG-BAFF followed by biotinylated anti-FLAG M2 antibody (Sigma) and peroxidase-coupled streptavidin. .. Anti-EDAR antibodies (10 μg/lane) were analyzed by SDS-PAGE under reducing conditions followed by Coomassie Blue staining.

    Incubation:

    Article Title: A versatile papaya mosaic virus (PapMV) vaccine platform based on sortase-mediated antigen coupling
    Article Snippet: Plates were blocked with 150 µL per well of PBS/0.1% Tween-20/2% BSA, and washed three times with PBS/0.1% Tween-20, before adding mice sera in twofold serial dilutions starting at 1:50. .. Plates were incubated for 1.5 h at 37 °C, and washed four times in PBS/0.1% Tween-20 before adding the peroxidase-conjugated goat anti-mouse IgG or IgG2a secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania, USA). .. After washing the plates four times, peptide-specific antibodies were detected with the addition of TMB substrate (Fitzgerald Industries International, Acton, Massachusetts, USA).

    Article Title: Protective Epitopes of the Plasmodium falciparum SERA5 Malaria Vaccine Reside in Intrinsically Unstructured N-Terminal Repetitive Sequences
    Article Snippet: The plates were again washed three times with PBS/T prior to addition of serum samples or purified IgG prepared in 5% skim milk in PBS/T. .. After washing with PBS/T, peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000; or horseradish peroxidase-conjugated rabbit anti-human IgG antibody (A8792; Sigma-Aldrich Corp., St. Louis, MO) diluted 1∶2000; or peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000 in 5% skim milk in PBS/T was added to the plates and incubated at 37°C for 1 hour. .. The plates were washed and incubated with 100 µl freshly prepared citrate-phosphate buffer (pH 5.0) containing 0.2% hydrogen peroxide and OPD tablet (154-01673; Sigma-Aldrich Corp., St. Louis, MO) for 15 minutes.

    Affinity Purification:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

    Flow Cytometry:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

    Cytometry:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

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  • 94
    Jackson Immuno horseradish peroxidase conjugated goat anti mouse antibody
    Lamin and otefin colocalize in salivary gland nuclei. Salivary glands were dissected from third-instar larvae, fixed, and probed with affinity-purified polyclonal antibodies specific for otefin (A and D), monoclonal antilamin antibody 611A3A6 (B), and monoclonal antibody RL1 against O-linked sugar-containing glycoproteins (E). <t>FITC-conjugated</t> <t>goat-anti-mouse</t> IgG and Cy3-conjugated goat-anti-rabbit IgG were used as the secondary antibodies for the monoclonal and polyclonal antibodies, respectively. To assess colocalization visually in double-labeled salivary gland cells (panels A and B, and panels D and E), the images from the Cy3 channel and the FITC channel were combined into a 24-bit RGB image as the red and green components, respectively (C and F). The resulting color image was yellow where the red and green features overlapped. Bar, 10 μm.
    Horseradish Peroxidase Conjugated Goat Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti mouse antibody/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti mouse antibody - by Bioz Stars, 2021-06
    94/100 stars
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    99
    Jackson Immuno gam hrp linked antibody
    Complex forming ability of hSLC41A1 in HEK293-(FLAG-SLC41A1) cells. FLAG-SLC41A1 was immunodetected with M2 anti-FLAG: <t>GAM-HRP</t> antibodies. A , gradient BN-PAGE (4-12%) analysis of SLC41A1 complexes. Successively increasing SDS concentration (as indicated)
    Gam Hrp Linked Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gam hrp linked antibody/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gam hrp linked antibody - by Bioz Stars, 2021-06
    99/100 stars
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    96
    Jackson Immuno peroxidase conjugated goat anti mouse igg
    Fig. 7. Binding of SNX17 and Dab1 to the intracellular domain of ApoER2. Microtiter plates (96-well) coated with a GST fusion protein containing the intracellular domain of ApoER2 were incubated ( A ) with the indicated amounts of purified <t>Dab1–MBP</t> expressed in E.coli or ( B ) with cell extracts derived from 293T cells expressing myc-tagged SNX17. Bound Dab1–MBP was visualized with the combination of an anti-MBP antibody and HRP-conjugated goat anti-rabbit <t>IgG.</t> Bound myc-SNX17 was visualized using the combination of an anti-myc antibody and HRP-conjugated goat anti-mouse IgG as described in Materials and methods. The color reaction was monitored by measuring absorption at 450 nm. ( C ) Competition of SNX17 binding by Dab1 was measured at two selected concentrations of myc-SNX17 (corresponding to 25 and 40% of cell lysate present in the incubation medium, respectively) in the absence and presence of 2 µg/ml Dab–MBP.
    Peroxidase Conjugated Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti mouse igg/product/Jackson Immuno
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti mouse igg - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    93
    Jackson Immuno peroxidase conjugated rabbit anti goat igg detection antibody
    Confirmation of antigen-specific <t>IgG</t> subclass levels by ELISA. (A) There were higher levels of anti-Hla IgG, IgG1, and IgG3 antibodies after S. aureus SSTI in the serum of BALB/c mice than in that of C57BL/6 mice. The higher levels of anti-Hla IgG (B)
    Peroxidase Conjugated Rabbit Anti Goat Igg Detection Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated rabbit anti goat igg detection antibody/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated rabbit anti goat igg detection antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Lamin and otefin colocalize in salivary gland nuclei. Salivary glands were dissected from third-instar larvae, fixed, and probed with affinity-purified polyclonal antibodies specific for otefin (A and D), monoclonal antilamin antibody 611A3A6 (B), and monoclonal antibody RL1 against O-linked sugar-containing glycoproteins (E). FITC-conjugated goat-anti-mouse IgG and Cy3-conjugated goat-anti-rabbit IgG were used as the secondary antibodies for the monoclonal and polyclonal antibodies, respectively. To assess colocalization visually in double-labeled salivary gland cells (panels A and B, and panels D and E), the images from the Cy3 channel and the FITC channel were combined into a 24-bit RGB image as the red and green components, respectively (C and F). The resulting color image was yellow where the red and green features overlapped. Bar, 10 μm.

    Journal: Molecular and Cellular Biology

    Article Title: Interactions among Drosophila Nuclear Envelope Proteins Lamin, Otefin, and YA

    doi:

    Figure Lengend Snippet: Lamin and otefin colocalize in salivary gland nuclei. Salivary glands were dissected from third-instar larvae, fixed, and probed with affinity-purified polyclonal antibodies specific for otefin (A and D), monoclonal antilamin antibody 611A3A6 (B), and monoclonal antibody RL1 against O-linked sugar-containing glycoproteins (E). FITC-conjugated goat-anti-mouse IgG and Cy3-conjugated goat-anti-rabbit IgG were used as the secondary antibodies for the monoclonal and polyclonal antibodies, respectively. To assess colocalization visually in double-labeled salivary gland cells (panels A and B, and panels D and E), the images from the Cy3 channel and the FITC channel were combined into a 24-bit RGB image as the red and green components, respectively (C and F). The resulting color image was yellow where the red and green features overlapped. Bar, 10 μm.

    Article Snippet: Fluorescein isothiocyanate (FITC) conjugated goat-anti-mouse IgG, Cy3A-conjugated goat-anti-rabbit IgG, and horseradish peroxidase-conjugated goat-anti-mouse antibody were purchased from Jackson ImmunoResearch Labs, West Grove, Pa. Anti- Drosophila tubulin antibody ( ) was a kind gift of T. Karr.

    Techniques: Affinity Purification, Labeling

    Complex forming ability of hSLC41A1 in HEK293-(FLAG-SLC41A1) cells. FLAG-SLC41A1 was immunodetected with M2 anti-FLAG: GAM-HRP antibodies. A , gradient BN-PAGE (4-12%) analysis of SLC41A1 complexes. Successively increasing SDS concentration (as indicated)

    Journal:

    Article Title: SLC41A1 Is a Novel Mammalian Mg2+ Carrier *

    doi: 10.1074/jbc.M707276200

    Figure Lengend Snippet: Complex forming ability of hSLC41A1 in HEK293-(FLAG-SLC41A1) cells. FLAG-SLC41A1 was immunodetected with M2 anti-FLAG: GAM-HRP antibodies. A , gradient BN-PAGE (4-12%) analysis of SLC41A1 complexes. Successively increasing SDS concentration (as indicated)

    Article Snippet: Both, total lysate proteins and membrane fraction proteins had been resolved by 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and immunodecorated with anti-FLAG antibody coupled to HRP (Invitrogen), or with antibody to β-actin (AbCam, Cambridge, UK) conjugated to GAM HRP-linked antibody (Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Polyacrylamide Gel Electrophoresis, Concentration Assay

    Overexpression of His-hSLC41A1 (56 kDa) in Salmonella strain MM281. His-tagged protein was immunodetected with His 6 tag: GAM-HRP antibodies, or His 6 tag: GAM-κ-HRP antibodies. Bands corresponding to recombinant His-hSLC41A1 immunodetected in

    Journal:

    Article Title: SLC41A1 Is a Novel Mammalian Mg2+ Carrier *

    doi: 10.1074/jbc.M707276200

    Figure Lengend Snippet: Overexpression of His-hSLC41A1 (56 kDa) in Salmonella strain MM281. His-tagged protein was immunodetected with His 6 tag: GAM-HRP antibodies, or His 6 tag: GAM-κ-HRP antibodies. Bands corresponding to recombinant His-hSLC41A1 immunodetected in

    Article Snippet: Both, total lysate proteins and membrane fraction proteins had been resolved by 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and immunodecorated with anti-FLAG antibody coupled to HRP (Invitrogen), or with antibody to β-actin (AbCam, Cambridge, UK) conjugated to GAM HRP-linked antibody (Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Over Expression, Recombinant

    Fig. 7. Binding of SNX17 and Dab1 to the intracellular domain of ApoER2. Microtiter plates (96-well) coated with a GST fusion protein containing the intracellular domain of ApoER2 were incubated ( A ) with the indicated amounts of purified Dab1–MBP expressed in E.coli or ( B ) with cell extracts derived from 293T cells expressing myc-tagged SNX17. Bound Dab1–MBP was visualized with the combination of an anti-MBP antibody and HRP-conjugated goat anti-rabbit IgG. Bound myc-SNX17 was visualized using the combination of an anti-myc antibody and HRP-conjugated goat anti-mouse IgG as described in Materials and methods. The color reaction was monitored by measuring absorption at 450 nm. ( C ) Competition of SNX17 binding by Dab1 was measured at two selected concentrations of myc-SNX17 (corresponding to 25 and 40% of cell lysate present in the incubation medium, respectively) in the absence and presence of 2 µg/ml Dab–MBP.

    Journal: The EMBO Journal

    Article Title: The PX-domain protein SNX17 interacts with members of the LDL receptor family and modulates endocytosis of the LDL receptor

    doi: 10.1093/emboj/cdf435

    Figure Lengend Snippet: Fig. 7. Binding of SNX17 and Dab1 to the intracellular domain of ApoER2. Microtiter plates (96-well) coated with a GST fusion protein containing the intracellular domain of ApoER2 were incubated ( A ) with the indicated amounts of purified Dab1–MBP expressed in E.coli or ( B ) with cell extracts derived from 293T cells expressing myc-tagged SNX17. Bound Dab1–MBP was visualized with the combination of an anti-MBP antibody and HRP-conjugated goat anti-rabbit IgG. Bound myc-SNX17 was visualized using the combination of an anti-myc antibody and HRP-conjugated goat anti-mouse IgG as described in Materials and methods. The color reaction was monitored by measuring absorption at 450 nm. ( C ) Competition of SNX17 binding by Dab1 was measured at two selected concentrations of myc-SNX17 (corresponding to 25 and 40% of cell lysate present in the incubation medium, respectively) in the absence and presence of 2 µg/ml Dab–MBP.

    Article Snippet: The following antibodies were obtained commercially from the sources indicated: anti-HA tag (16B12; Babco), anti-Myc (9E10, used as hybridoma supernatant from the corresponding cells; ATCC), anti-EEA1 (Affinity Bioreagents, Inc.), Oregon Green 488-labeled goat anti-mouse IgG (Molecular Probes), Alexa 594-labeled goat anti-rabbit IgG (Molecular Probes), goat anti-rabbit biotinylated IgG (Sigma), anti-MBP (E8030S; New England Biolabs), peroxidase- conjugated goat anti-mouse IgG (Jackson Immuno Research Laboratories) and peroxidase-conjugated anti-protein A (Sigma).

    Techniques: Binding Assay, Incubation, Purification, Derivative Assay, Expressing

    Confirmation of antigen-specific IgG subclass levels by ELISA. (A) There were higher levels of anti-Hla IgG, IgG1, and IgG3 antibodies after S. aureus SSTI in the serum of BALB/c mice than in that of C57BL/6 mice. The higher levels of anti-Hla IgG (B)

    Journal: Infection and Immunity

    Article Title: Proteomic Identification of saeRS-Dependent Targets Critical for Protective Humoral Immunity against Staphylococcus aureus Skin Infection

    doi: 10.1128/IAI.00667-15

    Figure Lengend Snippet: Confirmation of antigen-specific IgG subclass levels by ELISA. (A) There were higher levels of anti-Hla IgG, IgG1, and IgG3 antibodies after S. aureus SSTI in the serum of BALB/c mice than in that of C57BL/6 mice. The higher levels of anti-Hla IgG (B)

    Article Snippet: For the IgG subclasses, incubation was performed with goat anti-mouse IgG1, Ig2a, IgG2b, or IgG3 (1:1,000; Sigma-Aldrich), followed by incubation with the horseradish peroxidase-conjugated rabbit anti-goat IgG detection antibody (1:5,000; Jackson ImmunoResearch).

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay