peroxidase conjugated anti rabbit  (Boster Bio)

Journal: International Journal of Biological Macromolecules

Article Title: Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

doi: 10.1016/j.ijbiomac.2020.06.036

Figure Lengend Snippet: Immunohistochemistry (IHC) staining of PSCK isozymes in various tissues after bacterial infection. The size bars indicate 30 μm for A, B and E; 150 μm for C and D. CMN, cardiac myocytes nuclear; FbN, fibroblast nuclear; MF, Myocardial fiber; KC, Kupffer's cells; HN, Hepatocyte nuclear; RP, red pulp; WP, white pulp; CA, central artery; SN, splenic nodule; PLS; periarterial lymphatic sheath; PCT, proximal convoluted tubule; DCT, distal convoluted tubule; G, glomus; IV, interlobular veins; SMF, skeletal muscle fiber. The primary antibody was used as CK-B (1:100), CK-M (1:100) and CK-S (1:100). The sections were incubated with Rabbit IgG SABC Kit (Boster, Wuhan, China, SA1022). Reaction products were visualized by addition of chromogenic substrate 0.1% diaminobenzidine solution (Sigma-Aldrich, St. Louis, MO, USA) in 0.1% hydrogen peroxide in PBS and the slides counterstained with Harris hematoxylin lightly. The slides finally were examined by light microscopy (Nikon 80i, Tokyo, Japan). Negative controls were performed with diluent normal rabbit serum (1:500) instead of the primary antibody.

Article Snippet: After rinsing with 0.01 M PBS, the sections were incubated with Rabbit IgG SABC Kit (Boster, Wuhan, China, SA1022).

Techniques: Immunohistochemistry, Staining, Infection, Incubation, Light Microscopy

Journal: AMB Express

Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

doi: 10.1186/s13568-020-01042-2

Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

Techniques: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

Journal: AMB Express

Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

doi: 10.1186/s13568-020-01042-2

Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

Techniques: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

Journal: AMB Express

Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

doi: 10.1186/s13568-020-01042-2

Figure Lengend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

Techniques: Far Western Blot, Electrofocusing, SDS Page, Incubation

Journal: Frontiers in Neuroscience

Article Title: Redistribution of Histone Marks on Inflammatory Genes Associated With Intracerebral Hemorrhage-Induced Acute Brain Injury in Aging Rats

doi: 10.3389/fnins.2022.639656

Figure Lengend Snippet: Anti-IFN-γ reduces acute brain injury in aging ICH rats. (A) The neurological deficit scores of 22-month-old ICH rats after treatment with anti-IFN-γ antibody or normal IgG ( n = 12). (B) The mortality of aging ICH rats after treatment with anti-IFN-γ antibody or normal IgG ( n = 20). (C) Level of brain water contents in perihematomal brain tissues in 22-month-old ICH rats 3 days after treatment with anti-IFN-γ antibody or normal IgG ( n = 4). (D) Hematoxylin and eosin (H E) staining of perihematomal brain tissue derived from 22-month-old ICH rats 3 days after treatment with anti-IFN-γ antibody or normal IgG. Scale bars, 200 μm at the top and 100 μm at the bottom. (E) Fluoro-Jade B (FJB) staining and quantification of degenerative neural cells in perihematomal brain tissues derived from 22-month-old ICH rats 3 days after treatment with an anti-IFN-γ antibody or normal IgG. Scale bars, 100 μm at the top and 50 μm at the bottom. (F) TUNEL assay and quantification of apoptotic neural cells in perihematomal brain tissues derived from 22-month-old ICH rats 3 days after treatment with anti-IFN-γ antibody or normal IgG. Scale bars, 100 μm at the top and 50 μm at the bottom. (G) Nissl staining and quantification of Nissl bodies in perihematomal brain tissues derived from 22-month-old ICH rats 3 days after treatment with anti-IFN-γ antibody or normal IgG. Scale bars, 200 μm at the top and 50 μm at the bottom. The bar graphs show the mean ± SEM. p -value determined using the Student's t -test. * p

Article Snippet: On the following day, the sections were washed with PBS three times and then incubated with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) (Boster, Beijing, China) at a dilution of 1:500 for 1 h at 37°C.

Techniques: Staining, Derivative Assay, TUNEL Assay

Journal: Veterinary Research

Article Title: Specific EtMIC3-binding peptides inhibit Eimeria tenella sporozoites entry into host cells

doi: 10.1186/s13567-020-00873-y

Figure Lengend Snippet: Detection of binding capability between selected phages amd EtMIC3-bc1 protein. EtMIC3-bc1-coated plate was incubated with thirty selected phages. The washed plate was then successively incubated with rabbit anti-M13 polyclonal antibody and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG. Then substrate solution (0.01% H 2 O 2 and 1 mg/mL o-phenylenediamine) was added, and the reaction was stopped with 2 M sulfuric acid. The values of optical density (OD) at 490 nm was recorded. The blank control (BC), the negative control (NC) phages from G protein of porcine Vesicular Stomatitis Virus (VSV) (stored in our lab), and the positive control (PC) phages binding to EtAMA1 protein (stored in our lab) were used. Each sample was tested in triplicate. Values represent mean ± SD. Twenty-four selected phages clones showed higher binding capability than other clones (with the number of 1, 3, 13, 19, 23, 28), blank control and netative control phages ( P

Article Snippet: After washing, the plate was incubated with rabbit anti-EtMIC3 polyclonal antisera (60 μg/mL) diluted at 1:1000, reacted with 100 μL of HRP-labeled goat anti-rabbit IgG (1:5000) (Boster, Wuhan, China).

Techniques: Binding Assay, Incubation, Negative Control, Positive Control, Clone Assay