Journal: International Journal of Biological Macromolecules
Article Title: Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis
Figure Lengend Snippet: Immunohistochemistry (IHC) staining of PSCK isozymes in various tissues after bacterial infection. The size bars indicate 30 μm for A, B and E; 150 μm for C and D. CMN, cardiac myocytes nuclear; FbN, fibroblast nuclear; MF, Myocardial fiber; KC, Kupffer's cells; HN, Hepatocyte nuclear; RP, red pulp; WP, white pulp; CA, central artery; SN, splenic nodule; PLS; periarterial lymphatic sheath; PCT, proximal convoluted tubule; DCT, distal convoluted tubule; G, glomus; IV, interlobular veins; SMF, skeletal muscle fiber. The primary antibody was used as CK-B (1:100), CK-M (1:100) and CK-S (1:100). The sections were incubated with Rabbit IgG SABC Kit (Boster, Wuhan, China, SA1022). Reaction products were visualized by addition of chromogenic substrate 0.1% diaminobenzidine solution (Sigma-Aldrich, St. Louis, MO, USA) in 0.1% hydrogen peroxide in PBS and the slides counterstained with Harris hematoxylin lightly. The slides finally were examined by light microscopy (Nikon 80i, Tokyo, Japan). Negative controls were performed with diluent normal rabbit serum (1:500) instead of the primary antibody.
Article Snippet: After rinsing with 0.01 M PBS, the sections were incubated with Rabbit IgG SABC Kit (Boster, Wuhan, China, SA1022).
Techniques: Immunohistochemistry, Staining, Infection, Incubation, Light Microscopy