peroxidase affinipure goat anti mouse igg  (Jackson Immuno)

 
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    Name:
    Peroxidase AffiniPure Goat Anti Mouse IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    115-035-003
    Price:
    115.0
    Category:
    Whole IgG Affinity Purified Antibodies
    Conjugate:
    Horseradish Peroxidase
    Size:
    2 0 ml
    Format:
    Whole IgG
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno peroxidase affinipure goat anti mouse igg
    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 <t>IgG</t> titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated <t>affiniPure</t> goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg/product/Jackson Immuno
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase affinipure goat anti mouse igg - by Bioz Stars, 2021-06
    97/100 stars

    Images

    1) Product Images from "Protective Epitopes of the Plasmodium falciparum SERA5 Malaria Vaccine Reside in Intrinsically Unstructured N-Terminal Repetitive Sequences"

    Article Title: Protective Epitopes of the Plasmodium falciparum SERA5 Malaria Vaccine Reside in Intrinsically Unstructured N-Terminal Repetitive Sequences

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0098460

    Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 IgG titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .
    Figure Legend Snippet: Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I. ( A ) High, ( B ) medium-high, ( C ) medium-low, ( D ) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 IgG titers of the individual samples are in Table S2 . The patterns of reactivity for 18 individual sera are shown in Fig. S2 . Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. ( E ) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2 .

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

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    Article Snippet: Chemicals, antibodies, and plasmids We used blebbistatin (Sigma-Aldrich), 5-(4,6-dichlorotriazinyl)aminofluorescein (ThermoFisher Scientific), anti–UNC-45A (Enzo Life Sciences), anti-NMIIA (BioLegend), anti–p-NMII LC (Ser-20; Abcam), anti-NMIIB (Santa Cruz Biotechnology), anti-actin (Sigma-Aldrich), Texas Red–goat anti-mouse immunoglobulin G (IgG), Texas Red–goat anti-rabbit IgG, fluorescein isothiocyanate (FITC)–donkey and anti-mouse IgG, peroxidase–goat anti-mouse IgG, peroxidase–goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories), Neurobasal medium (Invitrogen), B-27 (Invitrogen), FGF2 (Invitrogen), mouse PDGHbb (Invitrogen), pRRLsinPPT, pMDLg/pRRE, pMD2.G, and pRSV-Rev plasmids (gifts from Alyson E. Fournier, Montreal Neurological Institute, Montreal, Canada), and Lenti-X concentrator (Clontech).

    Article Title: Generation and Characterization of Function-blocking Anti-ectodysplasin A (EDA) Monoclonal Antibodies That Induce Ectodermal Dysplasia *
    Article Snippet: Plates were revealed with serial dilutions of anti-EDA antibodies, followed by peroxidase-coupled goat anti-mouse IgG (1/8000).

    Construct:

    Article Title: Molecular and Therapeutic Characterization of Anti-ectodysplasin A Receptor (EDAR) Agonist Monoclonal Antibodies *
    Article Snippet: For epitope mapping, ELISA plates were coated with an F(ab′)2 fragment of a goat anti-human Ig (Jackson ImmunoResearch) to capture various EDAR-Fc constructs or a control receptor (B cell maturation antigen-Fc) in cell supernatants. .. EDAR-Fc constructs and B cell maturation antigen-Fc were revealed either with peroxidase-coupled donkey anti-human (H+L) (Jackson ImmunoResearch) or with anti-EDAR antibodies at 1 μg/ml followed by peroxidase-coupled goat anti-mouse IgG (Jackson ImmunoResearch) or with FLAG-EDA1 or FLAG-BAFF followed by biotinylated anti-FLAG M2 antibody (Sigma) and peroxidase-coupled streptavidin. .. Anti-EDAR antibodies (10 μg/lane) were analyzed by SDS-PAGE under reducing conditions followed by Coomassie Blue staining.

    Incubation:

    Article Title: A versatile papaya mosaic virus (PapMV) vaccine platform based on sortase-mediated antigen coupling
    Article Snippet: Plates were blocked with 150 µL per well of PBS/0.1% Tween-20/2% BSA, and washed three times with PBS/0.1% Tween-20, before adding mice sera in twofold serial dilutions starting at 1:50. .. Plates were incubated for 1.5 h at 37 °C, and washed four times in PBS/0.1% Tween-20 before adding the peroxidase-conjugated goat anti-mouse IgG or IgG2a secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania, USA). .. After washing the plates four times, peptide-specific antibodies were detected with the addition of TMB substrate (Fitzgerald Industries International, Acton, Massachusetts, USA).

    Article Title: Protective Epitopes of the Plasmodium falciparum SERA5 Malaria Vaccine Reside in Intrinsically Unstructured N-Terminal Repetitive Sequences
    Article Snippet: The plates were again washed three times with PBS/T prior to addition of serum samples or purified IgG prepared in 5% skim milk in PBS/T. .. After washing with PBS/T, peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000; or horseradish peroxidase-conjugated rabbit anti-human IgG antibody (A8792; Sigma-Aldrich Corp., St. Louis, MO) diluted 1∶2000; or peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000 in 5% skim milk in PBS/T was added to the plates and incubated at 37°C for 1 hour. .. The plates were washed and incubated with 100 µl freshly prepared citrate-phosphate buffer (pH 5.0) containing 0.2% hydrogen peroxide and OPD tablet (154-01673; Sigma-Aldrich Corp., St. Louis, MO) for 15 minutes.

    Affinity Purification:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

    Flow Cytometry:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

    Cytometry:

    Article Title: A cell surface receptor defined by a mAb mediates a unique type of cell death similar to oncosis
    Article Snippet: .. Affinity-purified goat anti-mouse Ig-fluorescein isothiocyanate (FITC) conjugate for flow cytometry and goat anti-mouse Ig-peroxidase conjugate for immunoblotting assays were purchased from Jackson ImmunoResearch. .. Propidium iodide and all other chemicals were obtained from Sigma unless otherwise indicated.

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    Jackson Immuno goat anti mouse immunoglobulin g igg
    Killing of wild-type and mutant strains of M. catarrhalis by human sera. (A) Effect of various sera on selected strains. The wild-type strain O35E (1), the uspA2 mutant O35EΔ2 (2), and the wild-type strain MC317 (3) were incubated for 30 min at 37°C in VBS ++ containing 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), or 10% (vol/vol) factor B-depleted human serum (c) or in VBS containing 10 mM MgCl 2 , 10 mM EGTA, and 10% (vol/vol) NHS (d). Portions of these reaction mixtures were plated at time zero and after 30 min, and the percentage of survival is calculated relative to the original inoculum. The data presented here are the means of results from three independent experiments plus the standard errors. (B) Involvement of <t>IgG</t> in killing of the uspA2 mutant O35EΔ2. Wild-type strain O35E (1) and the uspA2 mutant O35EΔ2 (2) were incubated with the following sera: 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), 10% (vol/vol) IgG-depleted NHS (c), and 10% (vol/vol) IgG-depleted NHS mixed with heat-inactivated NHS as a source of IgG (d). The data presented here are the means of results from three independent experiments plus the standard errors.
    Goat Anti Mouse Immunoglobulin G Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse immunoglobulin g igg/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Jackson Immuno anti mouse hrp
    Western blot for Myf6 protein in HCL and CLL cells. Nylon + (A) and PVDF (B) membranes were stained with murine Mab SC-514379 followed by <t>anti-mouse-HRP.</t> In C, PVDF was stained with <t>polyclonal</t> anti-GAPDH antibody followed by anti-mouse-HRP. Lanes include Myf6-transfected (lane 1) and untransfected (lane 2) 293 cells, nuclear and cytoplasmic fractions for CLL patient BL22 (lanes 3 4, respectively), HCL patient BL18 (lanes 5 6), and HCLv patient C276 (lanes 7 8). In each lane, 30 ug of total protein was added, except less in A lane 1 to obtain bands of similar intensity.
    Anti Mouse Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse hrp/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
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    93
    Jackson Immuno peroxidase affinipure goat anti mouse igg fcγ subclass 1 specific
    Antibody and T-cell responses after GX-19 vaccination in macaques. Macaques (n=3) were immunized with 3 mg of GX-19 as described in the methods. Serum and PBMCs were collected before (wk 0), during (wk 4, and 5.5) and after (wk 8) vaccination and were assessed for SARS-CoV-2 S-specific <t>IgG</t> antibodies by ELISA (A) and neutralizing antibodies against SARS-CoV-2 live-virus (B) . Data represent mean SEM of individual macaques, and dashed line indicate the assay limits of detection. The number of SARS-CoV-2 S-specific IFN-γ secreting cells in PBMCs was determined by IFN-γ ELISPOT assay after stimulation with peptide pools spanning the SARS-CoV-2 S protein. Shown are spot-forming cells (SFC) per 10 6 PBMCS in triplicate wells (C) . The frequency of S-specific CD4 + or CD8 + T cells producing IFN-γ, TNF-α, or IL-2 was determined by intracellular cytokine staining assays stimulated with SARS-CoV-2 S peptide pools. Shown are the frequency of S-specific CD4 + or CD8 + T cells after subtraction of background (DMSO vehicle) (D) .
    Peroxidase Affinipure Goat Anti Mouse Igg Fcγ Subclass 1 Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg fcγ subclass 1 specific/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
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    Jackson Immuno peroxidase affinipure goat anti mouse igg fcγ subclass 2a specific
    Analysis of the immune response to CORAVAX in mice. Balb/C mice were immunized once with live CORAVAX or twice (days 0 and 21) with 10 μg chemically inactivated particles of CORAVAX with or without adjuvant. Serum was collected from each mouse at days 14, 21, 28, 42, and 56 for analysis by a SARS-CoV-2 S-specific ELISA. a Immunization schedule: live CORAVAX used once on day 0; the inactivated vaccines were dosed on days 0 and 28 (figure created with BioRender.com). b SARS CoV-2 S1 <t>IgG</t> responses represented as EC50 titers c SARS CoV-2 RBD IgG responses on day 56 sera d SARS CoV-2 S1 <t>IgG2/1</t> isotype ratio. Two animals in the inactivated CORAVAX group did not make detectable <t>IgG1</t> antibodies.
    Peroxidase Affinipure Goat Anti Mouse Igg Fcγ Subclass 2a Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase affinipure goat anti mouse igg fcγ subclass 2a specific/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
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    Image Search Results


    Killing of wild-type and mutant strains of M. catarrhalis by human sera. (A) Effect of various sera on selected strains. The wild-type strain O35E (1), the uspA2 mutant O35EΔ2 (2), and the wild-type strain MC317 (3) were incubated for 30 min at 37°C in VBS ++ containing 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), or 10% (vol/vol) factor B-depleted human serum (c) or in VBS containing 10 mM MgCl 2 , 10 mM EGTA, and 10% (vol/vol) NHS (d). Portions of these reaction mixtures were plated at time zero and after 30 min, and the percentage of survival is calculated relative to the original inoculum. The data presented here are the means of results from three independent experiments plus the standard errors. (B) Involvement of IgG in killing of the uspA2 mutant O35EΔ2. Wild-type strain O35E (1) and the uspA2 mutant O35EΔ2 (2) were incubated with the following sera: 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), 10% (vol/vol) IgG-depleted NHS (c), and 10% (vol/vol) IgG-depleted NHS mixed with heat-inactivated NHS as a source of IgG (d). The data presented here are the means of results from three independent experiments plus the standard errors.

    Journal: Infection and Immunity

    Article Title: The UspA2 Protein of Moraxella catarrhalis Is Directly Involved in the Expression of Serum Resistance

    doi: 10.1128/IAI.73.4.2400-2410.2005

    Figure Lengend Snippet: Killing of wild-type and mutant strains of M. catarrhalis by human sera. (A) Effect of various sera on selected strains. The wild-type strain O35E (1), the uspA2 mutant O35EΔ2 (2), and the wild-type strain MC317 (3) were incubated for 30 min at 37°C in VBS ++ containing 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), or 10% (vol/vol) factor B-depleted human serum (c) or in VBS containing 10 mM MgCl 2 , 10 mM EGTA, and 10% (vol/vol) NHS (d). Portions of these reaction mixtures were plated at time zero and after 30 min, and the percentage of survival is calculated relative to the original inoculum. The data presented here are the means of results from three independent experiments plus the standard errors. (B) Involvement of IgG in killing of the uspA2 mutant O35EΔ2. Wild-type strain O35E (1) and the uspA2 mutant O35EΔ2 (2) were incubated with the following sera: 10% (vol/vol) heat-inactivated NHS (a), 10% (vol/vol) NHS (b), 10% (vol/vol) IgG-depleted NHS (c), and 10% (vol/vol) IgG-depleted NHS mixed with heat-inactivated NHS as a source of IgG (d). The data presented here are the means of results from three independent experiments plus the standard errors.

    Article Snippet: The secondary antibody used for Western blot analysis was goat anti-mouse immunoglobulin G (IgG) conjugated to horseradish peroxidase (Jackson ImmunoResearch, West Grove, Pa.).

    Techniques: Mutagenesis, Incubation

    Comparison of UspA2 proteins from M. catarrhalis strains O35E and MC317. (A) Alignment of the deduced amino acid sequences. Identical amino acids are shaded in dark gray, while conserved amino acids are shaded with light gray. This figure was generated by using the ClustalW Alignment program in MacVector (version 6.5). (B) Relative amounts of UspA2 exposed on the surface of the following strains as measured by the indirect antibody accessibility assay: the M. catarrhalis uspA1 uspA2 mutant O35E.12 (1), the uspA1 mutant O35E.1 (2), and the uspA1 mutant MC317.1 (3). The counts per minute of radioiodinated goat anti-mouse IgG bound to MAb 17C7 on the bacterial cell surface (a) are plotted on the y axis. MAb 3F12, a murine IgG MAb specific for the major outer membrane protein of H. ducreyi ), was used as the negative control (b). These data represent the means of results from two independent experiments plus standard deviations.

    Journal: Infection and Immunity

    Article Title: The UspA2 Protein of Moraxella catarrhalis Is Directly Involved in the Expression of Serum Resistance

    doi: 10.1128/IAI.73.4.2400-2410.2005

    Figure Lengend Snippet: Comparison of UspA2 proteins from M. catarrhalis strains O35E and MC317. (A) Alignment of the deduced amino acid sequences. Identical amino acids are shaded in dark gray, while conserved amino acids are shaded with light gray. This figure was generated by using the ClustalW Alignment program in MacVector (version 6.5). (B) Relative amounts of UspA2 exposed on the surface of the following strains as measured by the indirect antibody accessibility assay: the M. catarrhalis uspA1 uspA2 mutant O35E.12 (1), the uspA1 mutant O35E.1 (2), and the uspA1 mutant MC317.1 (3). The counts per minute of radioiodinated goat anti-mouse IgG bound to MAb 17C7 on the bacterial cell surface (a) are plotted on the y axis. MAb 3F12, a murine IgG MAb specific for the major outer membrane protein of H. ducreyi ), was used as the negative control (b). These data represent the means of results from two independent experiments plus standard deviations.

    Article Snippet: The secondary antibody used for Western blot analysis was goat anti-mouse immunoglobulin G (IgG) conjugated to horseradish peroxidase (Jackson ImmunoResearch, West Grove, Pa.).

    Techniques: Generated, Mutagenesis, Negative Control

    Western blot for Myf6 protein in HCL and CLL cells. Nylon + (A) and PVDF (B) membranes were stained with murine Mab SC-514379 followed by anti-mouse-HRP. In C, PVDF was stained with polyclonal anti-GAPDH antibody followed by anti-mouse-HRP. Lanes include Myf6-transfected (lane 1) and untransfected (lane 2) 293 cells, nuclear and cytoplasmic fractions for CLL patient BL22 (lanes 3 4, respectively), HCL patient BL18 (lanes 5 6), and HCLv patient C276 (lanes 7 8). In each lane, 30 ug of total protein was added, except less in A lane 1 to obtain bands of similar intensity.

    Journal: PLoS ONE

    Article Title: Expression of the muscle-associated gene MYF6 in hairy cell leukemia

    doi: 10.1371/journal.pone.0227586

    Figure Lengend Snippet: Western blot for Myf6 protein in HCL and CLL cells. Nylon + (A) and PVDF (B) membranes were stained with murine Mab SC-514379 followed by anti-mouse-HRP. In C, PVDF was stained with polyclonal anti-GAPDH antibody followed by anti-mouse-HRP. Lanes include Myf6-transfected (lane 1) and untransfected (lane 2) 293 cells, nuclear and cytoplasmic fractions for CLL patient BL22 (lanes 3 4, respectively), HCL patient BL18 (lanes 5 6), and HCLv patient C276 (lanes 7 8). In each lane, 30 ug of total protein was added, except less in A lane 1 to obtain bands of similar intensity.

    Article Snippet: GAPDH was detected on Nitrocellulose membranes using polyclonal antibody #9485 (Abcam, Cambridge, MA) followed by anti-mouse-HRP #115-035-166 (Jackson ImmunoResearch).

    Techniques: Western Blot, Staining, Transfection

    Antibody and T-cell responses after GX-19 vaccination in macaques. Macaques (n=3) were immunized with 3 mg of GX-19 as described in the methods. Serum and PBMCs were collected before (wk 0), during (wk 4, and 5.5) and after (wk 8) vaccination and were assessed for SARS-CoV-2 S-specific IgG antibodies by ELISA (A) and neutralizing antibodies against SARS-CoV-2 live-virus (B) . Data represent mean SEM of individual macaques, and dashed line indicate the assay limits of detection. The number of SARS-CoV-2 S-specific IFN-γ secreting cells in PBMCs was determined by IFN-γ ELISPOT assay after stimulation with peptide pools spanning the SARS-CoV-2 S protein. Shown are spot-forming cells (SFC) per 10 6 PBMCS in triplicate wells (C) . The frequency of S-specific CD4 + or CD8 + T cells producing IFN-γ, TNF-α, or IL-2 was determined by intracellular cytokine staining assays stimulated with SARS-CoV-2 S peptide pools. Shown are the frequency of S-specific CD4 + or CD8 + T cells after subtraction of background (DMSO vehicle) (D) .

    Journal: bioRxiv

    Article Title: Soluble Spike DNA vaccine provides long-term protective immunity against SAR-CoV-2 in mice and nonhuman primates

    doi: 10.1101/2020.10.09.334136

    Figure Lengend Snippet: Antibody and T-cell responses after GX-19 vaccination in macaques. Macaques (n=3) were immunized with 3 mg of GX-19 as described in the methods. Serum and PBMCs were collected before (wk 0), during (wk 4, and 5.5) and after (wk 8) vaccination and were assessed for SARS-CoV-2 S-specific IgG antibodies by ELISA (A) and neutralizing antibodies against SARS-CoV-2 live-virus (B) . Data represent mean SEM of individual macaques, and dashed line indicate the assay limits of detection. The number of SARS-CoV-2 S-specific IFN-γ secreting cells in PBMCs was determined by IFN-γ ELISPOT assay after stimulation with peptide pools spanning the SARS-CoV-2 S protein. Shown are spot-forming cells (SFC) per 10 6 PBMCS in triplicate wells (C) . The frequency of S-specific CD4 + or CD8 + T cells producing IFN-γ, TNF-α, or IL-2 was determined by intracellular cytokine staining assays stimulated with SARS-CoV-2 S peptide pools. Shown are the frequency of S-specific CD4 + or CD8 + T cells after subtraction of background (DMSO vehicle) (D) .

    Article Snippet: Following incubation, plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205), or IgG2a (Jackson ImmunoResearch Laboratories 115-035-206) or IgG2b (Jackson ImmunoResearch Laboratories 115-035-207) for the mouse sera/BAL or anti-monkey IgG (Bethyl Laborabories A140-102P) for the NHP sera for 1 hour at 37°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Staining

    Immunization with GX-19 elicit Th1-biased T cell responses in mice. BALB/c mice (n=3-7/group) were immunized at week 0 and 2 with indicated doses of GX-19 or pGX27 (empty control vector) as described in the methods (A-C) . Sera were collected 2 weeks post-boost and assessed for SARS-CoV-2 S-specific IgG1 and IgG2a/b. Endpoint titers (A) , and endpoint tier ratios of IgG2a/b to IgG1 (B) were calculated. 2 weeks post-boost mouse splenocytes were isolated and re-stimulated with peptide pools spanning the SARS-CoV-2 S protein ex vivo . Indicated cytokines in the supernatants of culture were quantified using Th1/Th2 cytometric bead array kit (D) . T cell responses were measured by IFN-γ ELISPOT in splenocytes stimulated with peptide pools spanning the SARS-CoV-2 S protein (C) . Cells were stained for intracellular production of IFN-γ, TNF-α, and IL-2. Shown are the frequency of S-specific CD4 + or CD8 + T cells after subtraction of background (DMSO vehicle) (E) . Data representative of two independent experiments. P values determined by Mann-Whitney test.

    Journal: bioRxiv

    Article Title: Soluble Spike DNA vaccine provides long-term protective immunity against SAR-CoV-2 in mice and nonhuman primates

    doi: 10.1101/2020.10.09.334136

    Figure Lengend Snippet: Immunization with GX-19 elicit Th1-biased T cell responses in mice. BALB/c mice (n=3-7/group) were immunized at week 0 and 2 with indicated doses of GX-19 or pGX27 (empty control vector) as described in the methods (A-C) . Sera were collected 2 weeks post-boost and assessed for SARS-CoV-2 S-specific IgG1 and IgG2a/b. Endpoint titers (A) , and endpoint tier ratios of IgG2a/b to IgG1 (B) were calculated. 2 weeks post-boost mouse splenocytes were isolated and re-stimulated with peptide pools spanning the SARS-CoV-2 S protein ex vivo . Indicated cytokines in the supernatants of culture were quantified using Th1/Th2 cytometric bead array kit (D) . T cell responses were measured by IFN-γ ELISPOT in splenocytes stimulated with peptide pools spanning the SARS-CoV-2 S protein (C) . Cells were stained for intracellular production of IFN-γ, TNF-α, and IL-2. Shown are the frequency of S-specific CD4 + or CD8 + T cells after subtraction of background (DMSO vehicle) (E) . Data representative of two independent experiments. P values determined by Mann-Whitney test.

    Article Snippet: Following incubation, plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205), or IgG2a (Jackson ImmunoResearch Laboratories 115-035-206) or IgG2b (Jackson ImmunoResearch Laboratories 115-035-207) for the mouse sera/BAL or anti-monkey IgG (Bethyl Laborabories A140-102P) for the NHP sera for 1 hour at 37°C.

    Techniques: Mouse Assay, Plasmid Preparation, Isolation, Ex Vivo, Enzyme-linked Immunospot, Staining, MANN-WHITNEY

    Diagram and immunogenicity of SARS-CoV-2 DNA vaccines. Schematic diagram of COVID-19 DNA vaccine expressing soluble SARS-CoV-2 S protein (S ΔTM ) or full-length SARS-CoV-2 S protein (S) (A) . BALB/c mice (n=4-10/group) were immunized at week 0 and 2 with pGX27-S ΔTM , pGX27-S or pGX27 (empty control vector) as described in the methods. Sera were collected 2 weeks post-prime (blue) and 2 weeks post-boost (red) and evaluated for SARS-CoV-2 S-specific IgG antibodies (B) .

    Journal: bioRxiv

    Article Title: Soluble Spike DNA vaccine provides long-term protective immunity against SAR-CoV-2 in mice and nonhuman primates

    doi: 10.1101/2020.10.09.334136

    Figure Lengend Snippet: Diagram and immunogenicity of SARS-CoV-2 DNA vaccines. Schematic diagram of COVID-19 DNA vaccine expressing soluble SARS-CoV-2 S protein (S ΔTM ) or full-length SARS-CoV-2 S protein (S) (A) . BALB/c mice (n=4-10/group) were immunized at week 0 and 2 with pGX27-S ΔTM , pGX27-S or pGX27 (empty control vector) as described in the methods. Sera were collected 2 weeks post-prime (blue) and 2 weeks post-boost (red) and evaluated for SARS-CoV-2 S-specific IgG antibodies (B) .

    Article Snippet: Following incubation, plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205), or IgG2a (Jackson ImmunoResearch Laboratories 115-035-206) or IgG2b (Jackson ImmunoResearch Laboratories 115-035-207) for the mouse sera/BAL or anti-monkey IgG (Bethyl Laborabories A140-102P) for the NHP sera for 1 hour at 37°C.

    Techniques: Expressing, Mouse Assay, Plasmid Preparation

    GX-19 elicit robust binding and neutralizing antibody responses in mice. BALB/c mice (n=4-7/group) were immunized at week 0 and 2 with indicated doses of GX-19 or pGX27 as described in the methods (A-C) . Sera were collected 2 weeks post-prime (blue) and 2 weeks post-boost (red) and assessed for SARS-CoV-2 S-specific IgG antibodies by ELISA (A) , and for post-boost sera, neutralizing antibodies against SARS-CoV-2 live-virus (C) . BAL were collected 2 weeks post-boost and assayed for SARS-CoV-2 S-specific IgG antibodies by ELISA (B) . Data representative of two independent experiments. P values determined by Mann-Whitney test.

    Journal: bioRxiv

    Article Title: Soluble Spike DNA vaccine provides long-term protective immunity against SAR-CoV-2 in mice and nonhuman primates

    doi: 10.1101/2020.10.09.334136

    Figure Lengend Snippet: GX-19 elicit robust binding and neutralizing antibody responses in mice. BALB/c mice (n=4-7/group) were immunized at week 0 and 2 with indicated doses of GX-19 or pGX27 as described in the methods (A-C) . Sera were collected 2 weeks post-prime (blue) and 2 weeks post-boost (red) and assessed for SARS-CoV-2 S-specific IgG antibodies by ELISA (A) , and for post-boost sera, neutralizing antibodies against SARS-CoV-2 live-virus (C) . BAL were collected 2 weeks post-boost and assayed for SARS-CoV-2 S-specific IgG antibodies by ELISA (B) . Data representative of two independent experiments. P values determined by Mann-Whitney test.

    Article Snippet: Following incubation, plates were washed 5 times with 0.05% PBST and then incubated with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories 115-035-003), IgG1 (Jackson ImmunoResearch Laboratories 115-035-205), or IgG2a (Jackson ImmunoResearch Laboratories 115-035-206) or IgG2b (Jackson ImmunoResearch Laboratories 115-035-207) for the mouse sera/BAL or anti-monkey IgG (Bethyl Laborabories A140-102P) for the NHP sera for 1 hour at 37°C.

    Techniques: Binding Assay, Mouse Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Analysis of the immune response to CORAVAX in mice. Balb/C mice were immunized once with live CORAVAX or twice (days 0 and 21) with 10 μg chemically inactivated particles of CORAVAX with or without adjuvant. Serum was collected from each mouse at days 14, 21, 28, 42, and 56 for analysis by a SARS-CoV-2 S-specific ELISA. a Immunization schedule: live CORAVAX used once on day 0; the inactivated vaccines were dosed on days 0 and 28 (figure created with BioRender.com). b SARS CoV-2 S1 IgG responses represented as EC50 titers c SARS CoV-2 RBD IgG responses on day 56 sera d SARS CoV-2 S1 IgG2/1 isotype ratio. Two animals in the inactivated CORAVAX group did not make detectable IgG1 antibodies.

    Journal: NPJ Vaccines

    Article Title: Rabies virus-based COVID-19 vaccine CORAVAX™ induces high levels of neutralizing antibodies against SARS-CoV-2

    doi: 10.1038/s41541-020-00248-6

    Figure Lengend Snippet: Analysis of the immune response to CORAVAX in mice. Balb/C mice were immunized once with live CORAVAX or twice (days 0 and 21) with 10 μg chemically inactivated particles of CORAVAX with or without adjuvant. Serum was collected from each mouse at days 14, 21, 28, 42, and 56 for analysis by a SARS-CoV-2 S-specific ELISA. a Immunization schedule: live CORAVAX used once on day 0; the inactivated vaccines were dosed on days 0 and 28 (figure created with BioRender.com). b SARS CoV-2 S1 IgG responses represented as EC50 titers c SARS CoV-2 RBD IgG responses on day 56 sera d SARS CoV-2 S1 IgG2/1 isotype ratio. Two animals in the inactivated CORAVAX group did not make detectable IgG1 antibodies.

    Article Snippet: Plates were washed three times the next day, followed by the addition of goat anti-mouse-IgG-Fc HRP antibody (Southern Biotech, Cat# 1033-05, 1:8000 in PBST) or goat anti-mouse-IgG1-Fc HRP antibody (Jackson ImmunoResearch, Cat# 115-035-205, 1:8000 in PBST) or goat anti-mouse-IgG2a-Fc HRP antibody (Jackson ImmunoResearch, Cat# 115-035-206, 1:8000 in PBST) for 2 h at RT.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay