peripheral blood mononuclear cells pbmcs  (Mediatech)

 
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    Mediatech peripheral blood mononuclear cells pbmcs
    Peripheral Blood Mononuclear Cells Pbmcs, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars

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    Article Title: Effect of Ribavirin on Viral Kinetics and Liver Gene Expression in Chronic Hepatitis C
    Article Snippet: Acid-Citrate-Dextrose-anticoagulated blood samples were drawn at week −4, 0, 6 hr, 24 hr, week 1 and week 4 and PBMC were separated immediately on Ficoll–Histopaque (Mediatech, Manassas, VA) density gradients.

    Article Title: Epigenetic priming of both tumor and NK cells augments antibody-dependent cellular cytotoxicity elicited by the anti-PD-L1 antibody avelumab against multiple carcinoma cell types
    Article Snippet: PBMCs were defrosted and rested overnight at 37 °C/5% CO2 in Iscove’s DMEM medium (Mediatech) supplemented with 10% heat-inactivated human AB serum (Omega Scientific, Terzana, CA, USA, virus negative, sterile filtered), 2 mM glutamine (Mediatech), 100 units per ml penicillin and 100 μg ml−1 streptomycin (Mediatech).

    Article Title: A Reproducible Immunopotency Assay to Measure Mesenchymal Stromal Cell Mediated T cell Suppression
    Article Snippet: PBMCs were plated at 5 × 104 cells/cm2 and grown in alpha-MEM (Mediatech, Manassas, VA) supplemented with 10% FBS (Life Technologies, Grand Island, NY) and 1x GlutaMax (Life Technologies).

    Article Title: Feasibility and Safety of RNA-transfected CD20-specific Chimeric Antigen Receptor T Cells in Dogs with Spontaneous B Cell Lymphoma
    Article Snippet: PBMCs were washed twice in T cell media (TCM) containing RPMI 1640 with 2 mmol/l L-Glutamine (Mediatech), 10% heat-inactivated fetal bovine serum (Atlanta Biologicals), 10 mmol/l HEPES (Gibco), and 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco), filtered through a 0.22 μm filter Stericup (Millipore).

    Article Title: An evaluation of methods correcting for cell-type heterogeneity in DNA methylation studies
    Article Snippet: PBMCs were separated with lymphocyte separation medium (Mediatech, Inc.).

    Article Title: Immunization with analogue peptide in combination with CpG and Montanide expands tumor antigen-specific CD8+ T cells in melanoma patients
    Article Snippet: PBMCs from melanoma patients were collected using a Ficoll gradient and were frozen in Iscove's DMEM medium (Mediatech Inc., Herndon, VA, USA) containing 10% human serum (Sigma-Aldrich, St. Louis, MO, USA) and 10% DMSO (FisherBiotech, Fair Lawn, NJ, USA) in liquid nitrogen until further processing.

    Article Title: Type III IFNs are produced by and stimulate human plasmacytoid dendritic cells 1
    Article Snippet: The PBMC were resuspended in RPMI 1640 medium (Mediatech Inc, Herndon, VA) containing 10% heat-inactivated fetal bovine serum (FBS), 2 mM l -glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin (Gemini Bio-Products Inc., West Sacramento, CA) and 25 mM HEPES, and counted with a Coulter Particle Counter Z1 (Beckman Coulter).

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    • pbmc isolation peripheral blood mononuclear cells pbmcs  (Mediatech)
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    Mediatech pbmc isolation peripheral blood mononuclear cells pbmcs
    S1P modulation reduces transmigration of <t>PBMCs</t> across an in vitro BBB model under shear stress. When hBMVEC/hAST co-culture was exposed to T I in the presence of S1P (50 nM), pFTY (50 nM), or AUY (50 nM), the ability of PBMCs to migrate through the barrier is reduced if compared to the condition in which the barrier is exposed only to cytokines. Data are mean of 2 independent experiments and are expressed as percent reduction in <t>PBMC</t> migration after 1 hour compared to T I. *p
    Pbmc Isolation Peripheral Blood Mononuclear Cells Pbmcs, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc isolation peripheral blood mononuclear cells pbmcs/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc isolation peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier
    peripheral blood mononuclear cells pbmcs  (Mediatech)
    86
    Mediatech peripheral blood mononuclear cells pbmcs
    S1P modulation reduces transmigration of <t>PBMCs</t> across an in vitro BBB model under shear stress. When hBMVEC/hAST co-culture was exposed to T I in the presence of S1P (50 nM), pFTY (50 nM), or AUY (50 nM), the ability of PBMCs to migrate through the barrier is reduced if compared to the condition in which the barrier is exposed only to cytokines. Data are mean of 2 independent experiments and are expressed as percent reduction in <t>PBMC</t> migration after 1 hour compared to T I. *p
    Peripheral Blood Mononuclear Cells Pbmcs, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier

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    S1P modulation reduces transmigration of PBMCs across an in vitro BBB model under shear stress. When hBMVEC/hAST co-culture was exposed to T I in the presence of S1P (50 nM), pFTY (50 nM), or AUY (50 nM), the ability of PBMCs to migrate through the barrier is reduced if compared to the condition in which the barrier is exposed only to cytokines. Data are mean of 2 independent experiments and are expressed as percent reduction in PBMC migration after 1 hour compared to T I. *p

    Journal: PLoS ONE

    Article Title: Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?

    doi: 10.1371/journal.pone.0133392

    Figure Lengend Snippet: S1P modulation reduces transmigration of PBMCs across an in vitro BBB model under shear stress. When hBMVEC/hAST co-culture was exposed to T I in the presence of S1P (50 nM), pFTY (50 nM), or AUY (50 nM), the ability of PBMCs to migrate through the barrier is reduced if compared to the condition in which the barrier is exposed only to cytokines. Data are mean of 2 independent experiments and are expressed as percent reduction in PBMC migration after 1 hour compared to T I. *p

    Article Snippet: PBMC isolation Peripheral blood mononuclear cells (PBMCs) were isolated from fresh heparinized blood of healthy subjects by density centrifugation with Lymphocyte Separation Medium (Mediatech, Herndon, VA) as previously described [ ].

    Techniques: Transmigration Assay, In Vitro, Co-Culture Assay, Migration