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Biochrom peripheral blood mononuclear cells pbmcs
Peripheral Blood Mononuclear Cells Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
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Isolation:

Article Title: Influence of high density lipoprotein cholesterol levels on circulating monocytic angiogenic cells functions in individuals with type 2 diabetes mellitus
Article Snippet: .. PBMCs were isolated from blood of each individual with type 2 diabetes by Biocoll (Biochrom AG; density = 1.077 g/ml) density-gradient centrifugation. .. Total PBMCs were seeded on 2 µg/cm2 fibronectin coated culture dishes (BD Falcon™) or on Lab-Tek ® II chamber slides system (Sigma-Aldrich Ltd, Poole, Dorset, UK) after red cell lyses.

Article Title: CAR-T Cells Targeting Epstein-Barr Virus gp350 Validated in a Humanized Mouse Model of EBV Infection and Lymphoproliferative Disease
Article Snippet: .. PBMCs were isolated using Ficoll density gradient separation (Biocoll Separating Solution; Biochrom, Berlin, Germany) and used for production of CAR-T cells and LCLs. ..

Centrifugation:

Article Title: Influence of high density lipoprotein cholesterol levels on circulating monocytic angiogenic cells functions in individuals with type 2 diabetes mellitus
Article Snippet: .. PBMCs were isolated from blood of each individual with type 2 diabetes by Biocoll (Biochrom AG; density = 1.077 g/ml) density-gradient centrifugation. .. Total PBMCs were seeded on 2 µg/cm2 fibronectin coated culture dishes (BD Falcon™) or on Lab-Tek ® II chamber slides system (Sigma-Aldrich Ltd, Poole, Dorset, UK) after red cell lyses.

Article Title: Cross-sectional analysis of CD8 T cell immunity to human herpesvirus 6B
Article Snippet: .. PBMCs were obtained by centrifugation on Ficoll/Hypaque (Biochrom). .. High-resolution HLA typing was performed by PCR-based methods (MVZ, Martinsried, Germany).

Cell Culture:

Article Title: Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells
Article Snippet: Fresh human PBMCs were isolated from blood from voluntary healthy donors by standard Pancoll density-gradient centrifugation (PAN-Biotech GmbH, Aidenbach, Germany). .. PBMCs were directly cultured in RPMI 1640 (Biochrom, Berlin, Germany) supplemented with 10% fetal bovine serum. .. Blood sampling was approved by the ethics committee of the Medical Faculty Heidelberg and was done in accordance with their recommendations.

Generated:

Article Title: Efficient immunoaffinity chromatography of lymphocytes directly from whole blood
Article Snippet: Afterwards the cells were stimulated with CD3/CD28 Streptamers for T cell expansion (IBA GmbH, Göttingen Germany) according to manufacturer’s protocol for three days and proliferation was assessed using FACS analysis. .. PBMCs were generated using Biocoll Separating Solution (Dens 1.077 g/ml) (Biochrom GmbH, Berlin, Germany) according to manufacturer’s protocol. .. Cells were positively enriched using the LS columns and CD8 MicroBeads according to manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany).

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    Biochrom pbmc isolation peripheral blood mononuclear cells pbmcs
    Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) <t>PBMCs</t> from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.
    Pbmc Isolation Peripheral Blood Mononuclear Cells Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc isolation peripheral blood mononuclear cells pbmcs/product/Biochrom
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc isolation peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biochrom peripheral blood mononuclear cells pbmc
    miR-20b expression in T cells and in <t>PBMC</t> from natalizumab-associated progressive multifocal leukoencephalopathy (PML). (A) PBMC were isolated using a <t>Ficoll</t> gradient and CD4+ T cells were sorted with magnetic beads ( n = 9; randomly selected patients of our initial longitudinal cohort consisting of 17 patients). miR-20b expression (qPCR) was measured in the CD4+ and CD4-negative fraction and compared using t- test. (B) CD4+ cells were activated by plate-bound anti-CD3-antibody and soluble anti-CD28-antibody without further cytokines, termed “Th0”, and with IL-1 (10 ng/mL), IL-6 (10 ng/mL), IL-23 (10 ng/mL) and TGF- β (5 ng/mL), termed “Th17”, as well as freshly isolated CD4+ T cells, termed “fresh”. miR-20b levels were measured by qPCR. (C) miR-20b levels in PBMC from natalizumab-treated patients ( n = 9, left bar) were compared to miR-20b levels in two PBMC samples from patients with natalizumab-associated PML (each patient one bar).
    Peripheral Blood Mononuclear Cells Pbmc, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmc/product/Biochrom
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmc - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biochrom blood mononuclear cells pbmc
    ESAT-6 and CFP-10- induced cytokine response in FluoroSpot. ESAT-6 induced cytokine immune response in 200.000 <t>PBMCs/well</t> in participants with active tuberculosis (TB, circle, n = 18), past tuberculosis (past TB, inverted triangle, n = 10), latent infection with M . tuberculosis (LTBI, square, ESAT-6-induced n = 24, CFP-10-induced n = 22), EliSpot-negative individuals (control, triangle, ESAT-6-induced n = 17, CFP-10-induced n = 19) was analysed. Groups had been defined according to the combination of their ESAT-6 and CFP-10 induced <t>IFN-γ</t> EliSpot-IGRA test result and clinical data. The number of IL-2 + (A), INF-γ + (B), IL-2 + INF-γ - (C), IL-2 - INF-γ + (D) and IL-2 + INF-γ + (E) spot-forming cells (SFC) were enumerated by FluoroSpot. (F) Mean proportion of ESAT-6 (top row) and CFP-10 (bottom row) -specific cytokine secreting cells for individuals with tuberculosis, past tuberculosis and LTBI are depicted as pie charts (light grey = IL-2 + INF-γ - , black = IL-2 - INF-γ + and dark grey = IL-2 + INF-γ + secreting cells). Mann-Whitney U-test for non-parametric data was used for comparative analysis. A p-value of
    Blood Mononuclear Cells Pbmc, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood mononuclear cells pbmc/product/Biochrom
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    blood mononuclear cells pbmc - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biochrom peripheral blood pbmc
    Expression of mRNA for NGFR H and NGFR L demonstrated by PCR with cDNA from <t>CBMC</t> cultured with NGF (10 ng/ml) for 21 days and freshly isolated <t>PBMC</t> as positive control (representative results with cells from one out of three different donors): lane 1, DNA-ladder; lane 2, negative control for PCR (without c-DNA); lane 3, PBMC; lane 4, CBMC, cultured with NGF (10 ng/ml).
    Peripheral Blood Pbmc, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood pbmc/product/Biochrom
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood pbmc - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) PBMCs from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.

    Journal: Virology Journal

    Article Title: Tim-3 expression on peripheral T cell subsets correlates with disease progression in hepatitis B infection

    doi: 10.1186/1743-422X-8-113

    Figure Lengend Snippet: Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) PBMCs from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.

    Article Snippet: PBMC isolation Peripheral blood mononuclear cells (PBMCs) were isolated from fresh-heparinized blood by standard Ficoll-Hypaque density centrifugation (Biochrom, Berline, Germany).

    Techniques: Expressing, Infection, Staining, Negative Control, MANN-WHITNEY

    miR-20b expression in T cells and in PBMC from natalizumab-associated progressive multifocal leukoencephalopathy (PML). (A) PBMC were isolated using a Ficoll gradient and CD4+ T cells were sorted with magnetic beads ( n = 9; randomly selected patients of our initial longitudinal cohort consisting of 17 patients). miR-20b expression (qPCR) was measured in the CD4+ and CD4-negative fraction and compared using t- test. (B) CD4+ cells were activated by plate-bound anti-CD3-antibody and soluble anti-CD28-antibody without further cytokines, termed “Th0”, and with IL-1 (10 ng/mL), IL-6 (10 ng/mL), IL-23 (10 ng/mL) and TGF- β (5 ng/mL), termed “Th17”, as well as freshly isolated CD4+ T cells, termed “fresh”. miR-20b levels were measured by qPCR. (C) miR-20b levels in PBMC from natalizumab-treated patients ( n = 9, left bar) were compared to miR-20b levels in two PBMC samples from patients with natalizumab-associated PML (each patient one bar).

    Journal: Annals of Clinical and Translational Neurology

    Article Title: Natalizumab restores aberrant miRNA expression profile in multiple sclerosis and reveals a critical role for miR-20b

    doi: 10.1002/acn3.152

    Figure Lengend Snippet: miR-20b expression in T cells and in PBMC from natalizumab-associated progressive multifocal leukoencephalopathy (PML). (A) PBMC were isolated using a Ficoll gradient and CD4+ T cells were sorted with magnetic beads ( n = 9; randomly selected patients of our initial longitudinal cohort consisting of 17 patients). miR-20b expression (qPCR) was measured in the CD4+ and CD4-negative fraction and compared using t- test. (B) CD4+ cells were activated by plate-bound anti-CD3-antibody and soluble anti-CD28-antibody without further cytokines, termed “Th0”, and with IL-1 (10 ng/mL), IL-6 (10 ng/mL), IL-23 (10 ng/mL) and TGF- β (5 ng/mL), termed “Th17”, as well as freshly isolated CD4+ T cells, termed “fresh”. miR-20b levels were measured by qPCR. (C) miR-20b levels in PBMC from natalizumab-treated patients ( n = 9, left bar) were compared to miR-20b levels in two PBMC samples from patients with natalizumab-associated PML (each patient one bar).

    Article Snippet: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll (Biochrom, Berlin, Germany) gradient and CD4+ cells were isolated by magnetic bead separation using STEMCELL EasySep Human CD4+ T Cell Enrichment Kit according to the manufacturer’s instructions.

    Techniques: Expressing, Isolation, Magnetic Beads, Real-time Polymerase Chain Reaction

    ESAT-6 and CFP-10- induced cytokine response in FluoroSpot. ESAT-6 induced cytokine immune response in 200.000 PBMCs/well in participants with active tuberculosis (TB, circle, n = 18), past tuberculosis (past TB, inverted triangle, n = 10), latent infection with M . tuberculosis (LTBI, square, ESAT-6-induced n = 24, CFP-10-induced n = 22), EliSpot-negative individuals (control, triangle, ESAT-6-induced n = 17, CFP-10-induced n = 19) was analysed. Groups had been defined according to the combination of their ESAT-6 and CFP-10 induced IFN-γ EliSpot-IGRA test result and clinical data. The number of IL-2 + (A), INF-γ + (B), IL-2 + INF-γ - (C), IL-2 - INF-γ + (D) and IL-2 + INF-γ + (E) spot-forming cells (SFC) were enumerated by FluoroSpot. (F) Mean proportion of ESAT-6 (top row) and CFP-10 (bottom row) -specific cytokine secreting cells for individuals with tuberculosis, past tuberculosis and LTBI are depicted as pie charts (light grey = IL-2 + INF-γ - , black = IL-2 - INF-γ + and dark grey = IL-2 + INF-γ + secreting cells). Mann-Whitney U-test for non-parametric data was used for comparative analysis. A p-value of

    Journal: PLoS ONE

    Article Title: Combined Antigen-Specific Interferon-γ and Interleukin-2 Release Assay (FluoroSpot) for the Diagnosis of Mycobacterium tuberculosis Infection

    doi: 10.1371/journal.pone.0120006

    Figure Lengend Snippet: ESAT-6 and CFP-10- induced cytokine response in FluoroSpot. ESAT-6 induced cytokine immune response in 200.000 PBMCs/well in participants with active tuberculosis (TB, circle, n = 18), past tuberculosis (past TB, inverted triangle, n = 10), latent infection with M . tuberculosis (LTBI, square, ESAT-6-induced n = 24, CFP-10-induced n = 22), EliSpot-negative individuals (control, triangle, ESAT-6-induced n = 17, CFP-10-induced n = 19) was analysed. Groups had been defined according to the combination of their ESAT-6 and CFP-10 induced IFN-γ EliSpot-IGRA test result and clinical data. The number of IL-2 + (A), INF-γ + (B), IL-2 + INF-γ - (C), IL-2 - INF-γ + (D) and IL-2 + INF-γ + (E) spot-forming cells (SFC) were enumerated by FluoroSpot. (F) Mean proportion of ESAT-6 (top row) and CFP-10 (bottom row) -specific cytokine secreting cells for individuals with tuberculosis, past tuberculosis and LTBI are depicted as pie charts (light grey = IL-2 + INF-γ - , black = IL-2 - INF-γ + and dark grey = IL-2 + INF-γ + secreting cells). Mann-Whitney U-test for non-parametric data was used for comparative analysis. A p-value of

    Article Snippet: Detection of IL-2+ and IFN-γ+ secreting cells The cytokine response was studied on peripheral blood mononuclear cells (PBMC) isolated by Ficoll Hypaque density gradient centrifugation (Biochrom, Berlin, Germany).

    Techniques: Infection, Enzyme-linked Immunospot, MANN-WHITNEY

    Detection of INF-γ + in EliSpot-IGRA and their concordance with INF-γ + results in FluoroSpot. ESAT-6 (A) and CFP-10 (B) -induced INF-γ + immune response in 200.000 PBMCs/well in participants with active tuberculosis (TB, circle, n = 18), past tuberculosis (past TB, inverted triangle, n = 10), latent infection with M. tuberculosis (LTBI, square, ESAT-6-induced n = 24, CFP-10-induced = 22) and EliSpot-negative individuals (control, triangle, ESAT-6-induced n = 17, CFP-10-induced n = 19) was analysed. LTBI and controls had been defined according to their ESAT-6 and CFP-10-induced IFN-γ EliSpot-IGRA test result and clinical data. Number of INF-γ + spot-forming cells (SFC) was enumerated by EliSpot. ESAT-6 (C) and CFP-10 (D) induced- INF-γ + SFC in EliSpot-IGRA (solid symbols) and FluoroSpot (open symbols) were analysed as matched pairs (connected with lines), differences were calculated using Wilcoxon signed rank test. Correlation between the number of ESAT-6 (E) and CFP-10 (F) specific INF-γ + spot-forming cells (SFC) in PBMC of 69 donors detected by FluoroSpot and EliSpot-IGRA. Concordance between EliSpot-IGRA and FluoroSpot results were assessed using R 2 coefficient. Agreement by Bland–Altman test was expressed as mean difference (horizontal solid line) and 95% limits of agreement (dashed line) between ESAT-6 (G) and CFP-10 (H) induced- INF-γ + SFC in FluoroSpot compared to EliSpot-IGRA.

    Journal: PLoS ONE

    Article Title: Combined Antigen-Specific Interferon-γ and Interleukin-2 Release Assay (FluoroSpot) for the Diagnosis of Mycobacterium tuberculosis Infection

    doi: 10.1371/journal.pone.0120006

    Figure Lengend Snippet: Detection of INF-γ + in EliSpot-IGRA and their concordance with INF-γ + results in FluoroSpot. ESAT-6 (A) and CFP-10 (B) -induced INF-γ + immune response in 200.000 PBMCs/well in participants with active tuberculosis (TB, circle, n = 18), past tuberculosis (past TB, inverted triangle, n = 10), latent infection with M. tuberculosis (LTBI, square, ESAT-6-induced n = 24, CFP-10-induced = 22) and EliSpot-negative individuals (control, triangle, ESAT-6-induced n = 17, CFP-10-induced n = 19) was analysed. LTBI and controls had been defined according to their ESAT-6 and CFP-10-induced IFN-γ EliSpot-IGRA test result and clinical data. Number of INF-γ + spot-forming cells (SFC) was enumerated by EliSpot. ESAT-6 (C) and CFP-10 (D) induced- INF-γ + SFC in EliSpot-IGRA (solid symbols) and FluoroSpot (open symbols) were analysed as matched pairs (connected with lines), differences were calculated using Wilcoxon signed rank test. Correlation between the number of ESAT-6 (E) and CFP-10 (F) specific INF-γ + spot-forming cells (SFC) in PBMC of 69 donors detected by FluoroSpot and EliSpot-IGRA. Concordance between EliSpot-IGRA and FluoroSpot results were assessed using R 2 coefficient. Agreement by Bland–Altman test was expressed as mean difference (horizontal solid line) and 95% limits of agreement (dashed line) between ESAT-6 (G) and CFP-10 (H) induced- INF-γ + SFC in FluoroSpot compared to EliSpot-IGRA.

    Article Snippet: Detection of IL-2+ and IFN-γ+ secreting cells The cytokine response was studied on peripheral blood mononuclear cells (PBMC) isolated by Ficoll Hypaque density gradient centrifugation (Biochrom, Berlin, Germany).

    Techniques: Enzyme-linked Immunospot, Infection

    Expression of mRNA for NGFR H and NGFR L demonstrated by PCR with cDNA from CBMC cultured with NGF (10 ng/ml) for 21 days and freshly isolated PBMC as positive control (representative results with cells from one out of three different donors): lane 1, DNA-ladder; lane 2, negative control for PCR (without c-DNA); lane 3, PBMC; lane 4, CBMC, cultured with NGF (10 ng/ml).

    Journal: Immunology

    Article Title: Nerve growth factor-? induces mast-cell marker expression during in vitro culture of human umbilical cord blood cells

    doi: 10.1046/j.1365-2567.2000.00984.x

    Figure Lengend Snippet: Expression of mRNA for NGFR H and NGFR L demonstrated by PCR with cDNA from CBMC cultured with NGF (10 ng/ml) for 21 days and freshly isolated PBMC as positive control (representative results with cells from one out of three different donors): lane 1, DNA-ladder; lane 2, negative control for PCR (without c-DNA); lane 3, PBMC; lane 4, CBMC, cultured with NGF (10 ng/ml).

    Article Snippet: Mononuclear cells from umbilical cord blood (cord blood derived mast cells; CBMC) of 12 healthy donors or from normal peripheral blood (PBMC) were prepared by differ-ential centrifugation on Ficoll–Hypaque (Biochrom, Berlin, Germany), followed by overnight adherence to polystyrol flasks and subsequent removal of non-adherent cells in order to eliminate contaminating cells like lymphocytes.

    Techniques: Expressing, Polymerase Chain Reaction, Cell Culture, Isolation, Positive Control, Negative Control