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Astarte Biologics peripheral blood mononuclear cells pbmcs
Peripheral Blood Mononuclear Cells Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/Astarte Biologics
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
92/100 stars

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Incubation:

Article Title: HIV-1 Coinfection and Morphine Coexposure Severely Dysregulate Hepatitis C Virus-Induced Hepatic Proinflammatory Cytokine Release and Free Radical Production: Increased Pathogenesis Coincides with Uncoordinated Host Defenses ▿
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were purchased from Astarte Biologics (Redmond, WA) and maintained in RPMI medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin and incubated as indicated above. .. JFH1 HCV (genotype 2a infectious HCV isolate) and a replication-deficient mutant clone (JFH1/GND) were kindly provided by Takaji Wakita (National Institute for Infectious Diseases, Tokyo, Japan) and were prepared and used to infect Huh7.5.1 cells as previously reported ( , ).

other:

Article Title: 1E7-03, a low MW compound targeting host protein phosphatase-1, inhibits HIV-1 transcription
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purchased from Astarte Biologics (Redmond, WA, USA).

Article Title: PTHrP(12-48) Modulates the Bone Marrow Microenvironment and Suppresses Human Osteoclast Differentiation and Lifespan
Article Snippet: Peripheral blood mononuclear cells (PBMCs) (Astarte Biologics, Bothell, WA, USA) stored frozen in liquid nitrogen were thawed quickly at 37°C and resuspended by adding drop-wise to 9 mL of culture media (alpha-MEM [Gibco, Grand Island, NY, USA] + 10% FBS [Hy-Clone Laboratories, Inc., Logan, UT, USA]) + 1× antibiotics (penicillin/streptomycin; Fisher, Pittsburg, PA, USA).

Article Title: Microfluidic assembly of hydrogel-based immunogenic tumor spheroids for evaluation of anticancer therapies and biomarker release
Article Snippet: Peripheral Blood mononuclear cells (PBMCs) were obtained from Astarte bio (Astarte Biologics, Bothell, WA) and subsequently treated to generate activated PBMCs.

Cell Culture:

Article Title: Circulating Interleukin-8 levels explain breast cancer osteolysis in mice and humans
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were purchased (Astarte Biologics, Redmond, WA) and cultured towards osteoclast development as described previously [ ]. ..

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    Astarte Biologics ev treated pbmcs pha
    Tumor-derived extracellular vesicles (EVs) blunt migration of activated peripheral blood mononuclear cells <t>(PBMCs).</t> Migration chambers contained naïve <t>PBMCs+/-PHA</t> in FBS-free media in top well. Bottom chambers contained media with fetal bovine
    Ev Treated Pbmcs Pha, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ev treated pbmcs pha/product/Astarte Biologics
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ev treated pbmcs pha - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    90
    Astarte Biologics hiv infection human pbmcs
    IFN-α is induced after <t>HIV-1</t> infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in <t>PBMCs</t> isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.
    Hiv Infection Human Pbmcs, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv infection human pbmcs/product/Astarte Biologics
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv infection human pbmcs - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    Astarte Biologics pbmc cells
    Effects of Myricetin, Quercetin, and Pinocembrin on <t>HIV-1</t> MN and HIV-1 89.6 infection in <t>PBMC</t> cells and on cell viability. A, C ) quantification of HIV-1 p24 protein assessed by ELISA B, D ) cell viability after flavonoid treatment (n = 9). AZT: positive control; PBMC: negative control (only cells); HIV-1 MN and HIV-1 89.6: negative control (virus) and Veh: vehicle control. Values shown with an asterisk (*) are statistically significant, when compared with the vehicle control (infection inhibition) and PBMC cells (negative control) (cell viability).
    Pbmc Cells, supplied by Astarte Biologics, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc cells/product/Astarte Biologics
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbmc cells - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Tumor-derived extracellular vesicles (EVs) blunt migration of activated peripheral blood mononuclear cells (PBMCs). Migration chambers contained naïve PBMCs+/-PHA in FBS-free media in top well. Bottom chambers contained media with fetal bovine

    Journal: Neuro-Oncology

    Article Title: Glioma-derived extracellular vesicles selectively suppress immune responses

    doi: 10.1093/neuonc/nov170

    Figure Lengend Snippet: Tumor-derived extracellular vesicles (EVs) blunt migration of activated peripheral blood mononuclear cells (PBMCs). Migration chambers contained naïve PBMCs+/-PHA in FBS-free media in top well. Bottom chambers contained media with fetal bovine

    Article Snippet: To assess the re-stimulation capacity of EV-treated PBMCs (±PHA), cells were challenged with 2 μg/mL tetanus toxin (Astarte Biologics) in some experiments.

    Techniques: Derivative Assay, Migration

    Extracellular vesicle (EV) concentration differentially affects cytokine output and proliferation of (naïve) PHA-stimulated peripheral blood mononuclear cells (PBMCs). (A) ELISA IL-2 measurements (24 h) from PHA-stimulated Jurkats + various EV

    Journal: Neuro-Oncology

    Article Title: Glioma-derived extracellular vesicles selectively suppress immune responses

    doi: 10.1093/neuonc/nov170

    Figure Lengend Snippet: Extracellular vesicle (EV) concentration differentially affects cytokine output and proliferation of (naïve) PHA-stimulated peripheral blood mononuclear cells (PBMCs). (A) ELISA IL-2 measurements (24 h) from PHA-stimulated Jurkats + various EV

    Article Snippet: To assess the re-stimulation capacity of EV-treated PBMCs (±PHA), cells were challenged with 2 μg/mL tetanus toxin (Astarte Biologics) in some experiments.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    PHA-stimulated peripheral blood mononuclear cells exposed to low extracellular vesicle (EV) concentrations release an activated cytokine profile; high EV concentrations impair that activated profile. Culture media from PHA-treated and PHA/EV-treated T-cells

    Journal: Neuro-Oncology

    Article Title: Glioma-derived extracellular vesicles selectively suppress immune responses

    doi: 10.1093/neuonc/nov170

    Figure Lengend Snippet: PHA-stimulated peripheral blood mononuclear cells exposed to low extracellular vesicle (EV) concentrations release an activated cytokine profile; high EV concentrations impair that activated profile. Culture media from PHA-treated and PHA/EV-treated T-cells

    Article Snippet: To assess the re-stimulation capacity of EV-treated PBMCs (±PHA), cells were challenged with 2 μg/mL tetanus toxin (Astarte Biologics) in some experiments.

    Techniques:

    IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: IFN-α is induced after HIV-1 infection in BLT but not Hu-PBL mice, and pegasys treatment in Hu-PBL mice upregulates ISGs A. , B. Sera of HIV-1-infected BLT mice A. and Hu-PBL mice B. at the indicated time points were tested for expression of IFN-α by ELISA. In B. , PBL refers to uninfected Hu-PBL mice (negative control), PBL + HIV indicates infected mice (test group), and PBL + Plasmid indicates sera from Hu-PBL mice injected with IFN-α-encoding plasmid (positive control). For A. and B. , n = 6 mice/group. C. HIV-infected Hu-PBL mice were tested for expression of IFN-β by ELISA. The positive control was soluble IFN-β; n = 6 mice. D. ISG expression in PBMCs isolated from control and pegasys (200 ng)-injected mice at the indicated time points was tested by qRT-PCR; n = 4 mice. Cont, untreated control mice. IFN, pegasys-treated mice. Error bars = 1 SD. Statistical analysis was carried out using non-parametric Mann-Whitney test.

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Infection, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Negative Control, Plasmid Preparation, Injection, Positive Control, Isolation, Quantitative RT-PCR, MANN-WHITNEY

    Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Journal: Oncotarget

    Article Title: Gene therapy with plasmids encoding IFN-β or IFN-α14 confers long-term resistance to HIV-1 in humanized mice

    doi: 10.18632/oncotarget.12512

    Figure Lengend Snippet: Injection of plasmids expressing subtypes of IFN-I results in prolonged upregulation of ISGs A. Hu-PBL mice were hydrodynamically injected with plasmids encoding IFN-α2, −α6, −α8, −α14 (left panel), or -IFN-beta (right panel) subtypes, and their sera were tested for IFN-I by ELISA at the indicated times after injection. n = 5 mice/group. B. PBMCs obtained on the indicated days after plasmid injection were tested for upregulation of the indicated ISGs over time by qRT-PCR. C. PBMCs obtained on days 2 and 40 after plasmid injection were tested for expression of indicated HIV-1 restriction factors by qPCR. Kruskal Wallis test followed by Dunn's multiple comparison analysis was used for statistical evaluation. * p

    Article Snippet: Human PBMC reconstitution and HIV infection Human PBMCs were obtained from Astarte Biologics (Bothell, WA), and these cells (1×107 ) were injected through the tail vein into 6-8-week-old NSG mice.

    Techniques: Injection, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Effects of Myricetin, Quercetin, and Pinocembrin on HIV-1 MN and HIV-1 89.6 infection in PBMC cells and on cell viability. A, C ) quantification of HIV-1 p24 protein assessed by ELISA B, D ) cell viability after flavonoid treatment (n = 9). AZT: positive control; PBMC: negative control (only cells); HIV-1 MN and HIV-1 89.6: negative control (virus) and Veh: vehicle control. Values shown with an asterisk (*) are statistically significant, when compared with the vehicle control (infection inhibition) and PBMC cells (negative control) (cell viability).

    Journal: PLoS ONE

    Article Title: Anti-HIV-1 Activity of Flavonoid Myricetin on HIV-1 Infection in a Dual-Chamber In Vitro Model

    doi: 10.1371/journal.pone.0115323

    Figure Lengend Snippet: Effects of Myricetin, Quercetin, and Pinocembrin on HIV-1 MN and HIV-1 89.6 infection in PBMC cells and on cell viability. A, C ) quantification of HIV-1 p24 protein assessed by ELISA B, D ) cell viability after flavonoid treatment (n = 9). AZT: positive control; PBMC: negative control (only cells); HIV-1 MN and HIV-1 89.6: negative control (virus) and Veh: vehicle control. Values shown with an asterisk (*) are statistically significant, when compared with the vehicle control (infection inhibition) and PBMC cells (negative control) (cell viability).

    Article Snippet: In PBMC cells infected by HIV-1 89.6, Myricetin presented an IC50 of 22.91 µM, 12.3x higher than Quercetin, and 16.4x higher than Pinocembrin.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control, Inhibition