peripheral blood mononuclear cells pbmcs  (ATCC)


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  • 99
    Name:
    Primary Peripheral Blood Mononuclear Cells PBMC Normal Human
    Description:

    Catalog Number:
    pcs-800-011
    Price:
    None
    Applications:
    Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
    Host:
    Homo sapiens, human
    Cell Type:
    Mononuclear
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    Structured Review

    ATCC peripheral blood mononuclear cells pbmcs
    Analysis of expression of activation surface markers in <t>PBMCs</t> stimulated with A. hydrophila ATCC ® 7966 TM <t>OMVs.</t> PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells"

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.02765

    Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
    Figure Legend Snippet: Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    Techniques Used: Expressing, Activation Assay, Cell Culture, Flow Cytometry, Cytometry

    Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P
    Figure Legend Snippet: Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

    Techniques Used: Crocin Bleaching Assay

    Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P
    Figure Legend Snippet: Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

    Techniques Used: Expressing, Inhibition, Cell Culture, Flow Cytometry, Cytometry

    Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P
    Figure Legend Snippet: Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

    Techniques Used: Cell Culture, Flow Cytometry, Cytometry

    Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.
    Figure Legend Snippet: Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

    Techniques Used: Staining, Confocal Microscopy

    Related Articles

    Trypan Blue Exclusion Assay:

    Article Title: Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles
    Article Snippet: .. Cytotoxicity test The effects of Ag-MWCNTs on the viability of mouse liver hepatocytes (AML 12; Chungnam University, Daejeon Korea) and human peripheral blood mononuclear cells (PBMCs) (American Type Culture Collection [ATCC], Manassas, VA, USA) were evaluated using a trypan blue exclusion assay and a LIVE/DEAD® Viability/Cytotoxicity Kit. .. Cultured AML 12 cells and human PBMCs were plated in six-well plates (1×105 cells per well) in Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), respectively, each supplemented with 10% (v/v) fetal bovine serum and 1% sterile antibiotic.

    Isolation:

    Article Title: The HIV-1 Env gp120 Inner Domain Shapes the Phe43 Cavity and the CD4 Binding Site
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). .. Briefly, PBMCs were obtained by leukapheresis and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the instructions of the manufacturer (StemCell Technologies, Vancouver, BC, Canada) and were activated with phytohemagglutinin-L (10 μg/ml) for 48 h and then maintained in RPMI 1640 complete medium supplemented with recombinant interleukin-2 (rIL-2) (100 U/ml).

    Article Title: A New Family of Small-Molecule CD4-Mimetic Compounds Contacts Highly Conserved Aspartic Acid 368 of HIV-1 gp120 and Mediates Antibody-Dependent Cellular Cytotoxicity
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). ..

    Labeling:

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice
    Article Snippet: .. Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes. .. PBMCs were then washed 3 times with HAEC media and added to HAEC cells (150 000 labeled PBMCs per chamber).

    Cytotoxicity Assay:

    Article Title: Analogs of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide
    Article Snippet: .. Cytotoxicity assay Cytotoxic activity of the peptides was assessed against human peripheral blood mononuclear cells (PBMCs) and human non-small-cell lung adenocarcinoma A549 cells (ATCC CCL-185). .. PBMCs were isolated from buffy coats by conventional density gradient centrifugation.

    Incubation:

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice
    Article Snippet: .. Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes. .. PBMCs were then washed 3 times with HAEC media and added to HAEC cells (150 000 labeled PBMCs per chamber).

    Activity Assay:

    Article Title: Analogs of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide
    Article Snippet: .. Cytotoxicity assay Cytotoxic activity of the peptides was assessed against human peripheral blood mononuclear cells (PBMCs) and human non-small-cell lung adenocarcinoma A549 cells (ATCC CCL-185). .. PBMCs were isolated from buffy coats by conventional density gradient centrifugation.

    Cell Culture:

    Article Title: DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes
    Article Snippet: .. Cell culture Human peripheral blood mononuclear cells (PBMC,) and Jurkat cells were purchased from ATCC (PCS-800-011, Manassas, VA). ..

    Article Title: The HIV-1 Env gp120 Inner Domain Shapes the Phe43 Cavity and the CD4 Binding Site
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). .. Briefly, PBMCs were obtained by leukapheresis and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the instructions of the manufacturer (StemCell Technologies, Vancouver, BC, Canada) and were activated with phytohemagglutinin-L (10 μg/ml) for 48 h and then maintained in RPMI 1640 complete medium supplemented with recombinant interleukin-2 (rIL-2) (100 U/ml).

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming
    Article Snippet: .. Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively. .. Cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 µg/mL streptomycin, and 1% L‐glutamine at 37 °C in 5% CO2 .

    Article Title: A New Family of Small-Molecule CD4-Mimetic Compounds Contacts Highly Conserved Aspartic Acid 368 of HIV-1 gp120 and Mediates Antibody-Dependent Cellular Cytotoxicity
    Article Snippet: .. Primary human peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were isolated, activated, and cultured as previously described ( , ). ..

    Gradient Centrifugation:

    Article Title: Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages
    Article Snippet: .. THP-1 macrophages were differentiated from THP-1 monocytes by treatment with 50 nM PMA for 48 h. Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood from healthy volunteers by density gradient centrifugation using Histopaque-1077 , and all experimental protocols were performed in accordance with the guidelines and regulations provided and accepted by the Institutional Review Board of the Tri-Service General Hospital, National Defense Medical Center and the volunteers' informed consent (TSGH-IRB-2-106-05-190 and TSGH-IRB-2-106-05-009). .. Mouse primary bone marrow derived macrophages (BMDM) were prepared from bone marrow collected from C57BL/6 mouse femur and tibia by differentiating in the M-CSF containing medium for 7 days.

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    ATCC fresh adult human pbmcs
    NK 2 homeobox 5 (Nkx2‐5) inhibits monocyte‐endothelial adhesion and decreases expression of adhesion molecules in early atherosclerosis. A, Upper panel: representative images used for quantification of peripheral blood monocytes ( <t>PBMC</t> ; green, <t>calcein</t> AM ) attached to aortic endothelial cells ( HAEC s, blue, DAPI ). Middle panel: effects of Nkx2‐5 on expression of vascular cell adhesion molecule‐1 ( VCAM ‐1) in endothelial cells (representative immunofluorescence image: green for VCAM ‐1 and blue for cell nuclei stained with DAPI ). Lower panel: effects of Nkx2‐5 on expression of E‐selectin in endothelial cells (representative immunofluorescence image: red for E‐selectin and blue for cell nuclei stained with DAPI ). B, Quantification of adhesion. The number of PBMC adhered to per 100 HAEC s was calculated. Results are expressed as a percent of values determined in the Ad‐ EV ‐treated group. Data represent the mean± SEM of 3 independent experiments. C, Representative immunoblot for intercellular adhesion molecule‐1 ( ICAM ‐1), VCAM ‐1, E‐selectin, and P‐selectin in endothelial cells infected with Ad‐ EV or Ad‐Nkx2‐5. D, Cross‐sections of early atherosclerotic lesions in aortic sinus were immunostained with antibodies against von Willebrand factor ( vWF ), VCAM ‐1, and E‐selectin. Staining with rabbit IgG isotype was used as the negative control. E, Quantification of histochemical staining of VCAM ‐1 and E‐selectin. Positive stained areas were quantified as a percentage of total plaque area or plaque endothelium. Data are expressed as mean± SEM (n=12 per group). AM indicates acetomethoxy; DAPI, 4′,6‐diamidino‐2‐phenylindole; HAECs, human aortic endothelial cells; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha.
    Fresh Adult Human Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fresh adult human pbmcs/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fresh adult human pbmcs - by Bioz Stars, 2020-09
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    pbmcs  (ATCC)
    93
    ATCC pbmcs
    NK degranulation assay. Surface expression of CD107a in resting (A) or pre-activated (B) <t>PBMCs.</t> Three culture conditions were analyzed: PBMCs alone (negative control), PBMCs with the addition of <t>K562</t> (positive control) and PBMCs with MSCs. For this flow cytometry assay, CD3 - CD56 + NK cells were gated. MSCs, mesenchymal stem cells; NK, natural killer, PBMCs, peripheral blood mononuclear cells.
    Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/ATCC
    Average 93 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    93/100 stars
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    Image Search Results


    NK 2 homeobox 5 (Nkx2‐5) inhibits monocyte‐endothelial adhesion and decreases expression of adhesion molecules in early atherosclerosis. A, Upper panel: representative images used for quantification of peripheral blood monocytes ( PBMC ; green, calcein AM ) attached to aortic endothelial cells ( HAEC s, blue, DAPI ). Middle panel: effects of Nkx2‐5 on expression of vascular cell adhesion molecule‐1 ( VCAM ‐1) in endothelial cells (representative immunofluorescence image: green for VCAM ‐1 and blue for cell nuclei stained with DAPI ). Lower panel: effects of Nkx2‐5 on expression of E‐selectin in endothelial cells (representative immunofluorescence image: red for E‐selectin and blue for cell nuclei stained with DAPI ). B, Quantification of adhesion. The number of PBMC adhered to per 100 HAEC s was calculated. Results are expressed as a percent of values determined in the Ad‐ EV ‐treated group. Data represent the mean± SEM of 3 independent experiments. C, Representative immunoblot for intercellular adhesion molecule‐1 ( ICAM ‐1), VCAM ‐1, E‐selectin, and P‐selectin in endothelial cells infected with Ad‐ EV or Ad‐Nkx2‐5. D, Cross‐sections of early atherosclerotic lesions in aortic sinus were immunostained with antibodies against von Willebrand factor ( vWF ), VCAM ‐1, and E‐selectin. Staining with rabbit IgG isotype was used as the negative control. E, Quantification of histochemical staining of VCAM ‐1 and E‐selectin. Positive stained areas were quantified as a percentage of total plaque area or plaque endothelium. Data are expressed as mean± SEM (n=12 per group). AM indicates acetomethoxy; DAPI, 4′,6‐diamidino‐2‐phenylindole; HAECs, human aortic endothelial cells; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Nkx2‐5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E–Deficient Mice

    doi: 10.1161/JAHA.116.004440

    Figure Lengend Snippet: NK 2 homeobox 5 (Nkx2‐5) inhibits monocyte‐endothelial adhesion and decreases expression of adhesion molecules in early atherosclerosis. A, Upper panel: representative images used for quantification of peripheral blood monocytes ( PBMC ; green, calcein AM ) attached to aortic endothelial cells ( HAEC s, blue, DAPI ). Middle panel: effects of Nkx2‐5 on expression of vascular cell adhesion molecule‐1 ( VCAM ‐1) in endothelial cells (representative immunofluorescence image: green for VCAM ‐1 and blue for cell nuclei stained with DAPI ). Lower panel: effects of Nkx2‐5 on expression of E‐selectin in endothelial cells (representative immunofluorescence image: red for E‐selectin and blue for cell nuclei stained with DAPI ). B, Quantification of adhesion. The number of PBMC adhered to per 100 HAEC s was calculated. Results are expressed as a percent of values determined in the Ad‐ EV ‐treated group. Data represent the mean± SEM of 3 independent experiments. C, Representative immunoblot for intercellular adhesion molecule‐1 ( ICAM ‐1), VCAM ‐1, E‐selectin, and P‐selectin in endothelial cells infected with Ad‐ EV or Ad‐Nkx2‐5. D, Cross‐sections of early atherosclerotic lesions in aortic sinus were immunostained with antibodies against von Willebrand factor ( vWF ), VCAM ‐1, and E‐selectin. Staining with rabbit IgG isotype was used as the negative control. E, Quantification of histochemical staining of VCAM ‐1 and E‐selectin. Positive stained areas were quantified as a percentage of total plaque area or plaque endothelium. Data are expressed as mean± SEM (n=12 per group). AM indicates acetomethoxy; DAPI, 4′,6‐diamidino‐2‐phenylindole; HAECs, human aortic endothelial cells; IgG, immunoglobulin G; TNFα, tumor necrosis factor alpha.

    Article Snippet: Fresh adult human PBMCs (PCS‐800‐011; ATCC) were labeled with calcein acetomethoxy (AM) dye: PBMCs were pelleted at 240g for 10 minutes, resuspended in 1 mL of culture medium with 2.5 μmol/L of calcein AM from the kit, and incubated at 37°C (5% CO2 ) for 30 minutes.

    Techniques: Expressing, Immunofluorescence, Staining, Infection, Negative Control

    S. sonnei induces the secretion of IL-1β, IL-18, NLRP3, ASC, and active caspase-1 in macrophages. (A) J774A.1 macrophages, THP-1 macrophages, PBMCs or BMDM were primed with 1 μg/ml LPS for 4 h and then infected with S. sonnei for an additional 20 h. The levels of IL-1β in the supernatants were measured by ELISA. (B–E) J774A.1 macrophages or THP-1 macrophages were primed with 1 μg/ml LPS for 4 h followed and then infected with S. sonnei for an additional 20 h or stimulated with 5 mM ATP for an additional 0.5 h. The levels of IL-1β (B) , IL-18 (C) , caspase-1 (D) , NLRP3, and ASC (E) in the supernatants were measured by Western blotting. The ELISA data are expressed as the mean ± SD of four separate experiments. The Western blotting results are representative of three different experiments. * and *** indicate significant differences at the levels of p

    Journal: Frontiers in Immunology

    Article Title: Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages

    doi: 10.3389/fimmu.2020.01115

    Figure Lengend Snippet: S. sonnei induces the secretion of IL-1β, IL-18, NLRP3, ASC, and active caspase-1 in macrophages. (A) J774A.1 macrophages, THP-1 macrophages, PBMCs or BMDM were primed with 1 μg/ml LPS for 4 h and then infected with S. sonnei for an additional 20 h. The levels of IL-1β in the supernatants were measured by ELISA. (B–E) J774A.1 macrophages or THP-1 macrophages were primed with 1 μg/ml LPS for 4 h followed and then infected with S. sonnei for an additional 20 h or stimulated with 5 mM ATP for an additional 0.5 h. The levels of IL-1β (B) , IL-18 (C) , caspase-1 (D) , NLRP3, and ASC (E) in the supernatants were measured by Western blotting. The ELISA data are expressed as the mean ± SD of four separate experiments. The Western blotting results are representative of three different experiments. * and *** indicate significant differences at the levels of p

    Article Snippet: THP-1 macrophages were differentiated from THP-1 monocytes by treatment with 50 nM PMA for 48 h. Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood from healthy volunteers by density gradient centrifugation using Histopaque-1077 , and all experimental protocols were performed in accordance with the guidelines and regulations provided and accepted by the Institutional Review Board of the Tri-Service General Hospital, National Defense Medical Center and the volunteers' informed consent (TSGH-IRB-2-106-05-190 and TSGH-IRB-2-106-05-009).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Western Blot

    Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p

    Journal: Turkish Journal of Hematology

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming

    doi: 10.4274/tjh.2018.0106

    Figure Lengend Snippet: Gene expression of tumor-suppressor genes. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for tumor-suppressor genes. GAPDH was used a reference gene and data were normalized to PBMCs. *p

    Article Snippet: Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively.

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

    Gene expression of reprogramming factors and pluripotency markers. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for A) reprogramming factors and B) pluripotency markers. GAPDH was used as a reference gene and data were normalized to PBMCs. *p

    Journal: Turkish Journal of Hematology

    Article Title: Tendency of K562 Chronic Myeloid Leukemia Cells Towards Cell Reprogramming

    doi: 10.4274/tjh.2018.0106

    Figure Lengend Snippet: Gene expression of reprogramming factors and pluripotency markers. RNA was isolated from peripheral blood mononuclear cells (PBMCs) and K562 cells and quantitative real-time polymerase chain reaction was performed. Relative gene expression was plotted for A) reprogramming factors and B) pluripotency markers. GAPDH was used as a reference gene and data were normalized to PBMCs. *p

    Article Snippet: Cell Culture Human CML cell line K562 and human peripheral blood mononuclear cells (PBMCs) were obtained from ATCC and Lonza, respectively.

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

    NK degranulation assay. Surface expression of CD107a in resting (A) or pre-activated (B) PBMCs. Three culture conditions were analyzed: PBMCs alone (negative control), PBMCs with the addition of K562 (positive control) and PBMCs with MSCs. For this flow cytometry assay, CD3 - CD56 + NK cells were gated. MSCs, mesenchymal stem cells; NK, natural killer, PBMCs, peripheral blood mononuclear cells.

    Journal: Stem Cell Research & Therapy

    Article Title: Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and their culture media

    doi: 10.1186/scrt392

    Figure Lengend Snippet: NK degranulation assay. Surface expression of CD107a in resting (A) or pre-activated (B) PBMCs. Three culture conditions were analyzed: PBMCs alone (negative control), PBMCs with the addition of K562 (positive control) and PBMCs with MSCs. For this flow cytometry assay, CD3 - CD56 + NK cells were gated. MSCs, mesenchymal stem cells; NK, natural killer, PBMCs, peripheral blood mononuclear cells.

    Article Snippet: An equal number of PBMCs was incubated with 2 × 105 K562 tumor cell line (ATCC CCL 243), as positive control of degranulation, or with medium alone (Iscove’s modified Dulbecco’s medium, IMDM), as negative control.

    Techniques: Degranulation Assay, Expressing, Negative Control, Positive Control, Flow Cytometry, Cytometry