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GE Healthcare peripheral blood mononuclear cells pbmc
Relative proliferation rates for PHA stimulated <t>PBMC</t> with the addition of no MSC, control MSC or <t>MIRB</t> labeled (20 μg Fe/ml) MSC (n =1 experiment).
Peripheral Blood Mononuclear Cells Pbmc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Mesenchymal stem cell labeling and in vitro MR characterization at 1.5 T of new SPIO contrast agent: Molday ION Rhodamine-B™"

Article Title: Mesenchymal stem cell labeling and in vitro MR characterization at 1.5 T of new SPIO contrast agent: Molday ION Rhodamine-B™

Journal: Contrast media & molecular imaging

doi: 10.1002/cmmi.396

Relative proliferation rates for PHA stimulated PBMC with the addition of no MSC, control MSC or MIRB labeled (20 μg Fe/ml) MSC (n =1 experiment).
Figure Legend Snippet: Relative proliferation rates for PHA stimulated PBMC with the addition of no MSC, control MSC or MIRB labeled (20 μg Fe/ml) MSC (n =1 experiment).

Techniques Used: Labeling

2) Product Images from "Nef Is Dispensable for Resistance of Simian Immunodeficiency Virus-Infected Macrophages to CD8+ T Cell Killing"

Article Title: Nef Is Dispensable for Resistance of Simian Immunodeficiency Virus-Infected Macrophages to CD8+ T Cell Killing

Journal: Journal of Virology

doi: 10.1128/JVI.01699-15

Ex vivo viral suppression assay scheme. (a) Target cells (macrophages and CD4 + T cells) were isolated from uninfected MHC-I-matched and -mismatched rhesus macaque PBMCs and infected in vitro . (b) Effector SIV-specific CD8 + T cells were enriched from SIV-infected
Figure Legend Snippet: Ex vivo viral suppression assay scheme. (a) Target cells (macrophages and CD4 + T cells) were isolated from uninfected MHC-I-matched and -mismatched rhesus macaque PBMCs and infected in vitro . (b) Effector SIV-specific CD8 + T cells were enriched from SIV-infected

Techniques Used: Ex Vivo, Suppression Assay, Isolation, Infection, In Vitro

3) Product Images from "Clonal Focusing of Epitope-Specific CD8+ T Lymphocytes in Rhesus Monkeys following Vaccination and Simian-Human Immunodeficiency Virus Challenge ▿"

Article Title: Clonal Focusing of Epitope-Specific CD8+ T Lymphocytes in Rhesus Monkeys following Vaccination and Simian-Human Immunodeficiency Virus Challenge ▿

Journal: Journal of Virology

doi: 10.1128/JVI.01038-07

TCR Vβ repertoires of vaccine-elicited p11C, p41A, or p68A epitope-specific CD8 + T-lymphocyte populations do not change following infection with a nonpathogenic SHIV. PBMC from monkeys (Mm) 90-98 (A), 128-97 (B), and 196-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6 infection. The lymphocytes were cultured in vitro with p11C, p41A, or p68A peptide; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8 + T-lymphocyte populations was used to evaluate Vβ repertoires.
Figure Legend Snippet: TCR Vβ repertoires of vaccine-elicited p11C, p41A, or p68A epitope-specific CD8 + T-lymphocyte populations do not change following infection with a nonpathogenic SHIV. PBMC from monkeys (Mm) 90-98 (A), 128-97 (B), and 196-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6 infection. The lymphocytes were cultured in vitro with p11C, p41A, or p68A peptide; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8 + T-lymphocyte populations was used to evaluate Vβ repertoires.

Techniques Used: Infection, Isolation, Cell Culture, In Vitro, Staining, Synthesized, Binding Assay

TCR Vβ repertoires of epitope-specific CD8 + T lymphocytes generated by vaccination are as diverse as those induced in response to pathogenic-SHIV infection. PBMC were isolated from monkeys vaccinated with a plasmid DNA prime/rMVA boost regimen (135-97, 128-97, 95-98, and 90-98) and from monkeys infected with SHIV-89.6P (134, 144, 146, and 153). These cells were stimulated in vitro with p11C or p41A peptide and then stained and sorted with p11C or p41A tetramer, respectively. cDNAs synthesized from the RNAs extracted from these tetramer-binding CD8 + T-lymphocyte populations were used to determine Vβ repertoires. The Vβ repertoires of p11C (A) and p41A (B) tetramer-sorted CD8 + T lymphocytes from vaccinated monkeys and p11C (C) and p41A (D) tetramer-sorted CD8 + T lymphocytes from infected monkeys are shown.
Figure Legend Snippet: TCR Vβ repertoires of epitope-specific CD8 + T lymphocytes generated by vaccination are as diverse as those induced in response to pathogenic-SHIV infection. PBMC were isolated from monkeys vaccinated with a plasmid DNA prime/rMVA boost regimen (135-97, 128-97, 95-98, and 90-98) and from monkeys infected with SHIV-89.6P (134, 144, 146, and 153). These cells were stimulated in vitro with p11C or p41A peptide and then stained and sorted with p11C or p41A tetramer, respectively. cDNAs synthesized from the RNAs extracted from these tetramer-binding CD8 + T-lymphocyte populations were used to determine Vβ repertoires. The Vβ repertoires of p11C (A) and p41A (B) tetramer-sorted CD8 + T lymphocytes from vaccinated monkeys and p11C (C) and p41A (D) tetramer-sorted CD8 + T lymphocytes from infected monkeys are shown.

Techniques Used: Generated, Infection, Isolation, Plasmid Preparation, In Vitro, Staining, Synthesized, Binding Assay

TCR Vβ repertoires of p11C and p41A epitope-specific CD8 + T-lymphocyte populations elicited by plasmid DNA/rMVA vaccination persist following rAd vaccination. PBMC isolated from vaccinated monkeys 90-98, 95-98, 128-97, and 135-97 1 month after rMVA and 1 year after rAd vaccination were stimulated in vitro with p11C and p41A peptides; stained with p11C or p41A tetramer, respectively; and then sorted. cDNAs synthesized from the RNAs extracted from these tetramer-binding CD8 + T-lymphocyte populations were used to evaluate Vβ repertoires. Shown are p11C-specific (A to D) and p41A-specific (E to H) CD8 + T lymphocytes.
Figure Legend Snippet: TCR Vβ repertoires of p11C and p41A epitope-specific CD8 + T-lymphocyte populations elicited by plasmid DNA/rMVA vaccination persist following rAd vaccination. PBMC isolated from vaccinated monkeys 90-98, 95-98, 128-97, and 135-97 1 month after rMVA and 1 year after rAd vaccination were stimulated in vitro with p11C and p41A peptides; stained with p11C or p41A tetramer, respectively; and then sorted. cDNAs synthesized from the RNAs extracted from these tetramer-binding CD8 + T-lymphocyte populations were used to evaluate Vβ repertoires. Shown are p11C-specific (A to D) and p41A-specific (E to H) CD8 + T lymphocytes.

Techniques Used: Plasmid Preparation, Isolation, In Vitro, Staining, Synthesized, Binding Assay

TCR Vβ repertoires of p11C and p41A epitope-specific CD8 + T lymphocytes focus transiently following infection with a pathogenic SHIV. PBMC from monkeys (Mm) 95-98 (A), 132-97 (B), and 135-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6P infection. The lymphocytes were cultured in vitro with p11C, p41A, and p68A peptides; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8 + T-lymphocyte populations was used to evaluate Vβ repertoires.
Figure Legend Snippet: TCR Vβ repertoires of p11C and p41A epitope-specific CD8 + T lymphocytes focus transiently following infection with a pathogenic SHIV. PBMC from monkeys (Mm) 95-98 (A), 132-97 (B), and 135-97 (C) were isolated 4 (wk4 pi) and 19 (wk19 pi) weeks following SHIV-89.6P infection. The lymphocytes were cultured in vitro with p11C, p41A, and p68A peptides; stained with tetramers; and sorted. cDNA synthesized from the RNA extracted from these tetramer-binding CD8 + T-lymphocyte populations was used to evaluate Vβ repertoires.

Techniques Used: Infection, Isolation, Cell Culture, In Vitro, Staining, Synthesized, Binding Assay

4) Product Images from "Superoxide-Generating Nox5α Is Functionally Required for the Human T-Cell Leukemia Virus Type 1-Induced Cell Transformation Phenotype"

Article Title: Superoxide-Generating Nox5α Is Functionally Required for the Human T-Cell Leukemia Virus Type 1-Induced Cell Transformation Phenotype

Journal: Journal of Virology

doi: 10.1128/JVI.00983-15

Analysis of Nox family expression in HTLV-1-infected T-cell lines and ATL PBMC. (A) Total RNAs were extracted from various HTLV-1-infected (MT1, MT2, MT4, and HUT102) and HTLV-1-uninfected (HUT78, H9, MOLT4, and MOLT17) T cells, and levels of mRNA expression
Figure Legend Snippet: Analysis of Nox family expression in HTLV-1-infected T-cell lines and ATL PBMC. (A) Total RNAs were extracted from various HTLV-1-infected (MT1, MT2, MT4, and HUT102) and HTLV-1-uninfected (HUT78, H9, MOLT4, and MOLT17) T cells, and levels of mRNA expression

Techniques Used: Expressing, Infection

5) Product Images from "Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification"

Article Title: Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification

Journal: PLoS ONE

doi: 10.1371/journal.pone.0074293

HMEC-1 and TIME show aberrant leukocyte-endothelial interactions under physiological shear stress. (A) Rolling and adhesion of PBMC and PMN on HMEC-1, TIME and HUVEC were analyzed using in vitro flow assay. The results are normalized to HUVEC (100%). (B) Absolute numbers of interacting (firmly adhering and transmigrating) leukocytes and (C) the transmigration percentage (the numbers of transmigrated cells divided by the numbers of interacting cells) on the three endothelial monolayers were determined. Data are shown as mean ± SEM (n = 3 for each assay, each with different leukocyte donors). *P ≤ 0.05. **P ≤ 0.01. ***P ≤ 0.001. (D) Images of representative endothelial monolayers 10 min. after start of PMN transmigration studies are shown. Phase contrast bright cells (representative cells indicated by white arrows) are located on the apical surface of the endothelial cells and phase contrast dark cells (representative cells indicated by white arrow-heads) are situated below the monolayer. Note that all three endothelial types form confluent intact monolayers. Inserts show adhering and transmigrating cells in more detail. Scale bars represent 100 µm.
Figure Legend Snippet: HMEC-1 and TIME show aberrant leukocyte-endothelial interactions under physiological shear stress. (A) Rolling and adhesion of PBMC and PMN on HMEC-1, TIME and HUVEC were analyzed using in vitro flow assay. The results are normalized to HUVEC (100%). (B) Absolute numbers of interacting (firmly adhering and transmigrating) leukocytes and (C) the transmigration percentage (the numbers of transmigrated cells divided by the numbers of interacting cells) on the three endothelial monolayers were determined. Data are shown as mean ± SEM (n = 3 for each assay, each with different leukocyte donors). *P ≤ 0.05. **P ≤ 0.01. ***P ≤ 0.001. (D) Images of representative endothelial monolayers 10 min. after start of PMN transmigration studies are shown. Phase contrast bright cells (representative cells indicated by white arrows) are located on the apical surface of the endothelial cells and phase contrast dark cells (representative cells indicated by white arrow-heads) are situated below the monolayer. Note that all three endothelial types form confluent intact monolayers. Inserts show adhering and transmigrating cells in more detail. Scale bars represent 100 µm.

Techniques Used: In Vitro, Flow Cytometry, Transmigration Assay

6) Product Images from "Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay ▿"

Article Title: Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay ▿

Journal: Clinical and Vaccine Immunology : CVI

doi: 10.1128/CVI.00044-09

The magnitude of IFN-γ responses in relation to different forms of TB. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) in response to ESAT-6, pool A, and pool B, which were determined in patients with different forms of TB, was compared by a one-way analysis of variance Newman-Keuls multiple comparison test. Data were expressed as means ± standard errors of the means. *, significant difference ( P
Figure Legend Snippet: The magnitude of IFN-γ responses in relation to different forms of TB. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) in response to ESAT-6, pool A, and pool B, which were determined in patients with different forms of TB, was compared by a one-way analysis of variance Newman-Keuls multiple comparison test. Data were expressed as means ± standard errors of the means. *, significant difference ( P

Techniques Used: Negative Control

IFN-γ responses to ESAT-6 determined by the ELISPOT assay were correlated with TB exposure in healthy donors. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) was plotted against TB exposure class.
Figure Legend Snippet: IFN-γ responses to ESAT-6 determined by the ELISPOT assay were correlated with TB exposure in healthy donors. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) was plotted against TB exposure class.

Techniques Used: Enzyme-linked Immunospot, Negative Control

The influence of anti-TB treatment on the magnitude of IFN-γ responses and sensitivity of ELISPOT assay. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) in response to ESAT-6 (A), pool A (B), and pool B (C) determined at different time points after anti-TB treatment in patients with active TB ( n = 46). (D) The sensitivities of the ELISPOT assay (calculated as responses to composite antigens) at different time points after anti-TB treatment. 2w, with 2 weeks; 1m, 1 month; 3m, 3 months; 6m, 6 months.
Figure Legend Snippet: The influence of anti-TB treatment on the magnitude of IFN-γ responses and sensitivity of ELISPOT assay. The number of IFN-γ SFC per 2 × 10 5 PBMC (minus the values of the negative control wells) in response to ESAT-6 (A), pool A (B), and pool B (C) determined at different time points after anti-TB treatment in patients with active TB ( n = 46). (D) The sensitivities of the ELISPOT assay (calculated as responses to composite antigens) at different time points after anti-TB treatment. 2w, with 2 weeks; 1m, 1 month; 3m, 3 months; 6m, 6 months.

Techniques Used: Enzyme-linked Immunospot, Negative Control

7) Product Images from "The Split Virus Influenza Vaccine rapidly activates immune cells through Fcγ Receptors"

Article Title: The Split Virus Influenza Vaccine rapidly activates immune cells through Fcγ Receptors

Journal: Vaccine

doi: 10.1016/j.vaccine.2014.07.115

SV signaling activity is induced by influenza protein derived immune complexes. (A) Whole-blood and PBMCs prepared via Ficoll density centrifugation were stimulated with either PBS, SV, or R848 at 10ug/ml for 30mins prior to fixation and flow cytometric
Figure Legend Snippet: SV signaling activity is induced by influenza protein derived immune complexes. (A) Whole-blood and PBMCs prepared via Ficoll density centrifugation were stimulated with either PBS, SV, or R848 at 10ug/ml for 30mins prior to fixation and flow cytometric

Techniques Used: Activity Assay, Derivative Assay, Centrifugation, Flow Cytometry

Related Articles

Isolation:

Article Title: Importance of Membrane Fusion Mediated by Human Immunodeficiency Virus Envelope Glycoproteins for Lysis of Primary CD4-Positive T Cells
Article Snippet: .. Whole human peripheral blood mononuclear cells (PBMC) were isolated from fresh blood by Ficoll-Paque (Amersham Pharmacia, Uppsala, Sweden) density centrifugation. ..

Article Title: Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation
Article Snippet: .. The accumulation of debris might have occurred due to the presence of differentiated germ cells or somatic cells in the distinct band isolated with Ficoll–Paque PLUS from spawning/postspawning fish. .. In Trial 2, when only the modified Lacerda et al. (2013) [ ] culture medium was used, better results were obtained in terms of meagre SSC viability and proliferation, and the cell cultures were prolonged to 41 days.

Article Title: Six‐gene Assay as a new biomarker in the blood of patients with colorectal cancer: establishment and clinical validation
Article Snippet: .. Mononuclear cells from each peripheral blood sample were isolated by density separation with Ficoll‐Paque (GE Healthcare, Little Chalfont, Buckinghamshire, UK). .. RNA was extracted from mononuclear cells using an RNeasy® Plus Micro Kit (Qiagen, Duesseldorf, Germany) according to the manufacturer's instructions.

Article Title: Ex Vivo Generation of Highly Purified and Activated Natural Killer Cells from Human Peripheral Blood
Article Snippet: .. PBMCs were isolated by gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare, Uppsala, Sweden), and were washed twice with phosphate-buffered saline (PBS) containing 2% FBS and 1 m M EDTA. .. The purified primary NK cells used as controls were obtained by an NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Centrifugation:

Article Title: Importance of Membrane Fusion Mediated by Human Immunodeficiency Virus Envelope Glycoproteins for Lysis of Primary CD4-Positive T Cells
Article Snippet: .. Whole human peripheral blood mononuclear cells (PBMC) were isolated from fresh blood by Ficoll-Paque (Amersham Pharmacia, Uppsala, Sweden) density centrifugation. ..

other:

Article Title: Prognostic Impact of Circulating Tumor Cell Detected Using a Novel Fluidic Cell Microarray Chip System in Patients with Breast Cancer
Article Snippet: Two milliliters of blood sample were processed using Ficoll-Paque PLUS (GE Healthcare, Little Chalfont, UK).

Fluorescence In Situ Hybridization:

Article Title: Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation
Article Snippet: .. The accumulation of debris might have occurred due to the presence of differentiated germ cells or somatic cells in the distinct band isolated with Ficoll–Paque PLUS from spawning/postspawning fish. .. In Trial 2, when only the modified Lacerda et al. (2013) [ ] culture medium was used, better results were obtained in terms of meagre SSC viability and proliferation, and the cell cultures were prolonged to 41 days.

Gradient Centrifugation:

Article Title: Ex Vivo Generation of Highly Purified and Activated Natural Killer Cells from Human Peripheral Blood
Article Snippet: .. PBMCs were isolated by gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare, Uppsala, Sweden), and were washed twice with phosphate-buffered saline (PBS) containing 2% FBS and 1 m M EDTA. .. The purified primary NK cells used as controls were obtained by an NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

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    GE Healthcare nucleofection peripheral blood mononuclear cells pbmcs
    Genetic analysis of GPM6A . ( a ) Sequencing strategy and overview of the detected variants. Displayed are the coding exons (filled boxes) and the noncoding region of GPM6A (empty box). Arrows indicate rare variants found. Frequencies of rare variants in cases (black) versus controls (gray) are given. ( b) Pedigrees of two claustrophobic individuals (SIWO and THKA), carrying the mutation at locus c.*1834 (position 2882 in human GPM6A transcript variant 1, mRNA; NM_005277.3), suggesting an association between this mutation and the claustrophobic phenotype. ( c ) Highly phylogenetically conserved genomic structure surrounding c.*1834T > C within the seed sequence of miR124 in the 3′untranslated region of GPM6A . ( d ) Expression analysis after miR124 <t>nucleofection.</t> Shown are the results of GPM6A RNA expression in peripheral blood mononuclear cells <t>(PBMCs)</t> after nucleofection with miR124 from two patients and six controls (that is, not carrying the variant; age, gender and disease matched; three controls per patient). Results were standardized to the results after just a pulse. ( e ) Restraint stress induces upregulation of miR124 in the amygdala of male mice, identifying this miR as a stress-regulated transcript ( N =22 per group).
    Nucleofection Peripheral Blood Mononuclear Cells Pbmcs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleofection peripheral blood mononuclear cells pbmcs/product/GE Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    GE Healthcare fiv specific pbmc proliferative activity
    Virus-specific lymphoproliferative activity in the study cats. At the times indicated relative to the first <t>FIV-MDC</t> inoculum, <t>PBMCs</t> were exposed to intact FIV-M2 virions (a) or to pooled FIV-M2 Gag oligopeptides (b) and then examined for 3 H-thymidine incorporation. Columns represent the stimulation index (S.I.) of individual animals, which was considered positive when above threshold dotted line (S.I. > 2). All cats responded to the nonspecific stimulus Concavalin A with counts per minute ranging from 10,000 to 38,000 counts per minute, while background proliferation in medium alone ranged between 100 and 1,100. At each sampling point, the animals are represented in the same order as shown in Figure 1.
    Fiv Specific Pbmc Proliferative Activity, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    94
    GE Healthcare pbmc
    Immune suppressive MDSC and M2 persistence in <t>PDAC-SIS-PDX</t> mice. Frequency of ( A ) MDSC (human CD45 + CD3 − CD56 − CD16 − HLADR low/− CD11b + CD33 + ), ( B ) IL-10 producing MDSC and ( C ) TGF-beta-producing MDSC, as well as ( D ) PDAC-SIS-PDX M2 macrophages (CD45 + CD3 − CD56 − CD14 + CD163 + ), ( E ) IL-10 producing M2 macrophages and ( F ) TGF-beta-producing M2 monocytes in <t>PBMC,</t> spleens and PDAC of PDAC-SIS-PDX mice 4-months post-transplant as determined by flow cytometry. N = 7 SIS-PDX mice from three genetically unrelated donor cohorts were analyzed.
    Pbmc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 428 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/GE Healthcare
    Average 94 stars, based on 428 article reviews
    Price from $9.99 to $1999.99
    pbmc - by Bioz Stars, 2020-09
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    Image Search Results


    Genetic analysis of GPM6A . ( a ) Sequencing strategy and overview of the detected variants. Displayed are the coding exons (filled boxes) and the noncoding region of GPM6A (empty box). Arrows indicate rare variants found. Frequencies of rare variants in cases (black) versus controls (gray) are given. ( b) Pedigrees of two claustrophobic individuals (SIWO and THKA), carrying the mutation at locus c.*1834 (position 2882 in human GPM6A transcript variant 1, mRNA; NM_005277.3), suggesting an association between this mutation and the claustrophobic phenotype. ( c ) Highly phylogenetically conserved genomic structure surrounding c.*1834T > C within the seed sequence of miR124 in the 3′untranslated region of GPM6A . ( d ) Expression analysis after miR124 nucleofection. Shown are the results of GPM6A RNA expression in peripheral blood mononuclear cells (PBMCs) after nucleofection with miR124 from two patients and six controls (that is, not carrying the variant; age, gender and disease matched; three controls per patient). Results were standardized to the results after just a pulse. ( e ) Restraint stress induces upregulation of miR124 in the amygdala of male mice, identifying this miR as a stress-regulated transcript ( N =22 per group).

    Journal: Translational Psychiatry

    Article Title: A single gene defect causing claustrophobia

    doi: 10.1038/tp.2013.28

    Figure Lengend Snippet: Genetic analysis of GPM6A . ( a ) Sequencing strategy and overview of the detected variants. Displayed are the coding exons (filled boxes) and the noncoding region of GPM6A (empty box). Arrows indicate rare variants found. Frequencies of rare variants in cases (black) versus controls (gray) are given. ( b) Pedigrees of two claustrophobic individuals (SIWO and THKA), carrying the mutation at locus c.*1834 (position 2882 in human GPM6A transcript variant 1, mRNA; NM_005277.3), suggesting an association between this mutation and the claustrophobic phenotype. ( c ) Highly phylogenetically conserved genomic structure surrounding c.*1834T > C within the seed sequence of miR124 in the 3′untranslated region of GPM6A . ( d ) Expression analysis after miR124 nucleofection. Shown are the results of GPM6A RNA expression in peripheral blood mononuclear cells (PBMCs) after nucleofection with miR124 from two patients and six controls (that is, not carrying the variant; age, gender and disease matched; three controls per patient). Results were standardized to the results after just a pulse. ( e ) Restraint stress induces upregulation of miR124 in the amygdala of male mice, identifying this miR as a stress-regulated transcript ( N =22 per group).

    Article Snippet: Expression analysis after nucleofection Peripheral blood mononuclear cells (PBMCs) of claustrophobic patients with the mutation in the 3′UTR (N =2) and three matches per subject were freshly isolated using the standard Ficoll-Paque Plus isolation procedure (GE Healthcare, Munich, Germany).

    Techniques: Sequencing, Mutagenesis, Variant Assay, Expressing, RNA Expression, Mouse Assay

    Virus-specific lymphoproliferative activity in the study cats. At the times indicated relative to the first FIV-MDC inoculum, PBMCs were exposed to intact FIV-M2 virions (a) or to pooled FIV-M2 Gag oligopeptides (b) and then examined for 3 H-thymidine incorporation. Columns represent the stimulation index (S.I.) of individual animals, which was considered positive when above threshold dotted line (S.I. > 2). All cats responded to the nonspecific stimulus Concavalin A with counts per minute ranging from 10,000 to 38,000 counts per minute, while background proliferation in medium alone ranged between 100 and 1,100. At each sampling point, the animals are represented in the same order as shown in Figure 1.

    Journal: Retrovirology

    Article Title: Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course

    doi: 10.1186/1742-4690-5-33

    Figure Lengend Snippet: Virus-specific lymphoproliferative activity in the study cats. At the times indicated relative to the first FIV-MDC inoculum, PBMCs were exposed to intact FIV-M2 virions (a) or to pooled FIV-M2 Gag oligopeptides (b) and then examined for 3 H-thymidine incorporation. Columns represent the stimulation index (S.I.) of individual animals, which was considered positive when above threshold dotted line (S.I. > 2). All cats responded to the nonspecific stimulus Concavalin A with counts per minute ranging from 10,000 to 38,000 counts per minute, while background proliferation in medium alone ranged between 100 and 1,100. At each sampling point, the animals are represented in the same order as shown in Figure 1.

    Article Snippet: FIV-specific PBMC proliferative activity was determined by incubating 1.5 × 105 PBMCs in 96-well plates in 200 μl medium supplemented with 10% human serum with either 1 μg/well of purified sonicated FIV-M2 or no stimulus for 4 days, and counting after addition of 3 H-thymidine (GE Healthcare, Milan, Italy) for 18 h. Results are reported as stimulation index (S.I.), that is the ratio of radioactivity incorporated by test PBMCs in the presence or absence of antigen, which was considered positive when > 2 [ ].

    Techniques: Activity Assay, Sampling

    Plasma viremia (a), proviral load in the PBMCs (b), and circulating CD4 + T lymphocyte percentages (c) of the study cats at the times indicated relative to the first FIV-MDC inoculum. CD4 + T cells were monitored by flow cytometry in peripheral blood by direct staining with anti-feline CD4-PE (clone vpg34, AbD Serotec, Raleigh, NC) for 30 min as previously described (8). Symbols represent individual animals. Arrows indicate the times of FIV-MDC inoculation.

    Journal: Retrovirology

    Article Title: Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course

    doi: 10.1186/1742-4690-5-33

    Figure Lengend Snippet: Plasma viremia (a), proviral load in the PBMCs (b), and circulating CD4 + T lymphocyte percentages (c) of the study cats at the times indicated relative to the first FIV-MDC inoculum. CD4 + T cells were monitored by flow cytometry in peripheral blood by direct staining with anti-feline CD4-PE (clone vpg34, AbD Serotec, Raleigh, NC) for 30 min as previously described (8). Symbols represent individual animals. Arrows indicate the times of FIV-MDC inoculation.

    Article Snippet: FIV-specific PBMC proliferative activity was determined by incubating 1.5 × 105 PBMCs in 96-well plates in 200 μl medium supplemented with 10% human serum with either 1 μg/well of purified sonicated FIV-M2 or no stimulus for 4 days, and counting after addition of 3 H-thymidine (GE Healthcare, Milan, Italy) for 18 h. Results are reported as stimulation index (S.I.), that is the ratio of radioactivity incorporated by test PBMCs in the presence or absence of antigen, which was considered positive when > 2 [ ].

    Techniques: Flow Cytometry, Cytometry, Staining

    Immune suppressive MDSC and M2 persistence in PDAC-SIS-PDX mice. Frequency of ( A ) MDSC (human CD45 + CD3 − CD56 − CD16 − HLADR low/− CD11b + CD33 + ), ( B ) IL-10 producing MDSC and ( C ) TGF-beta-producing MDSC, as well as ( D ) PDAC-SIS-PDX M2 macrophages (CD45 + CD3 − CD56 − CD14 + CD163 + ), ( E ) IL-10 producing M2 macrophages and ( F ) TGF-beta-producing M2 monocytes in PBMC, spleens and PDAC of PDAC-SIS-PDX mice 4-months post-transplant as determined by flow cytometry. N = 7 SIS-PDX mice from three genetically unrelated donor cohorts were analyzed.

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Immune suppressive MDSC and M2 persistence in PDAC-SIS-PDX mice. Frequency of ( A ) MDSC (human CD45 + CD3 − CD56 − CD16 − HLADR low/− CD11b + CD33 + ), ( B ) IL-10 producing MDSC and ( C ) TGF-beta-producing MDSC, as well as ( D ) PDAC-SIS-PDX M2 macrophages (CD45 + CD3 − CD56 − CD14 + CD163 + ), ( E ) IL-10 producing M2 macrophages and ( F ) TGF-beta-producing M2 monocytes in PBMC, spleens and PDAC of PDAC-SIS-PDX mice 4-months post-transplant as determined by flow cytometry. N = 7 SIS-PDX mice from three genetically unrelated donor cohorts were analyzed.

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry

    Long-term reconstitution of LUAD-SIS-PDX mice with human B cells and dendritic cells (DC). Frequencies of Human B cells (CD45+CD3−CD56−CD19+CD20+) and DC (CD45+CD3−CD56−CD19−CD11c+) in PBMC (“blood”), spleen and transplanted tumor of LUAD-SIS-PDX mice ( A ) 2- and ( B ) 6-months post tumor-engraftment, as determined by flow cytometry. Each dot represents one LUAD-SIS-PDX mouse, N= 10-15 animals per tissue.

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Long-term reconstitution of LUAD-SIS-PDX mice with human B cells and dendritic cells (DC). Frequencies of Human B cells (CD45+CD3−CD56−CD19+CD20+) and DC (CD45+CD3−CD56−CD19−CD11c+) in PBMC (“blood”), spleen and transplanted tumor of LUAD-SIS-PDX mice ( A ) 2- and ( B ) 6-months post tumor-engraftment, as determined by flow cytometry. Each dot represents one LUAD-SIS-PDX mouse, N= 10-15 animals per tissue.

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry

    Human T cells are exhausted in phenotype and enriched in T regulatory cells in NSCLC (LUAD). Immune phenotyping of human immune cells and T cell subsets in indicated tissues by flow cytometry. ( A, F ) Frequencies of human hematopoietic cells (human CD45 + ), ( B, G ) conventional T cells (CD45 + CD3 + CD56 − ), ( C, H ) PD-1, PD-L1 and/or PD-L2 expressing conventional T cells, ( D, I ) T helper (CD45 + CD3 + CD4 + CD8 − CD56 − ), and CTL CD45 + CD3 + CD4 − CD8 + CD56 − subsets, ( E, J ) T-regulatory cell (CD45 + CD3 + CD4 + Foxp-3 + CD8 − CD56 − ) subset in donor-matched lung vs. NSCLC (LUAD) ( A-E, top ), or patient derived PBMC, lung tissue and LUAD ( F-J, bottom ). Each data point represents one genetically unrelated human donor (N= 8-31 depending on tissue, same donors as for Figure 1 ). Paired t-test for donor-matched lung tissue vs. LUAD, one-way ANOVA with post-hoc Tukey test for comparisons between LUAD-patient derived PBMC, lung tissue and LUAD (not always donor matched between all three tissues). *p

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Human T cells are exhausted in phenotype and enriched in T regulatory cells in NSCLC (LUAD). Immune phenotyping of human immune cells and T cell subsets in indicated tissues by flow cytometry. ( A, F ) Frequencies of human hematopoietic cells (human CD45 + ), ( B, G ) conventional T cells (CD45 + CD3 + CD56 − ), ( C, H ) PD-1, PD-L1 and/or PD-L2 expressing conventional T cells, ( D, I ) T helper (CD45 + CD3 + CD4 + CD8 − CD56 − ), and CTL CD45 + CD3 + CD4 − CD8 + CD56 − subsets, ( E, J ) T-regulatory cell (CD45 + CD3 + CD4 + Foxp-3 + CD8 − CD56 − ) subset in donor-matched lung vs. NSCLC (LUAD) ( A-E, top ), or patient derived PBMC, lung tissue and LUAD ( F-J, bottom ). Each data point represents one genetically unrelated human donor (N= 8-31 depending on tissue, same donors as for Figure 1 ). Paired t-test for donor-matched lung tissue vs. LUAD, one-way ANOVA with post-hoc Tukey test for comparisons between LUAD-patient derived PBMC, lung tissue and LUAD (not always donor matched between all three tissues). *p

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Flow Cytometry, Expressing, Derivative Assay

    Immune cells in PDAC-SIS-PDX mice resemble exhausted TILs of PDAC donors. ( A-H ) Flow cytometry of human immune cells from PBMC, spleen, and transplanted PDAC of PDX mice analyzed 4-6 months post-transplant. ( A ) Frequency of human hematopoietic cells, ( B ) human NK cells (CD45 + CD56 + CD3 − ), and ( C ) expression of activating receptors CD16 and NKG2D as well as ( D ) markers of exhaustion PD-1, PD-L1 and PD-L2 on NK cells in PDX derived PBMC, spleen and transplanted PDAC. ( E ) Frequency of conventional T cells (CD45 + CD56 − CD3 + ) and ( E ) expression of PD-1, PD-L1 and PD-L2 on conventional T cells, ( G ) frequencies of Th (CD45 + CD3 + CD4 + CD8 − ), and CTL (CD45 + CD3 + CD4 − CD8 + ) as percent of T cells, and ( H ) Treg (CD45 + CD3 + CD4 − CD8 + Foxp3 + ) as percent of Th cell subsets in PDX derived PBMC, spleen, and transplanted PDAC. Each data point represents one PDAC-SIS-PDX mouse tissue (n= 12-33 PDX mice per group, seven donor-cohorts). Data are represented as mean SEM; Paired t-test for donor-matched lung tissue vs. PDAC, one-way ANOVA with post-hoc Tukey test *p

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Immune cells in PDAC-SIS-PDX mice resemble exhausted TILs of PDAC donors. ( A-H ) Flow cytometry of human immune cells from PBMC, spleen, and transplanted PDAC of PDX mice analyzed 4-6 months post-transplant. ( A ) Frequency of human hematopoietic cells, ( B ) human NK cells (CD45 + CD56 + CD3 − ), and ( C ) expression of activating receptors CD16 and NKG2D as well as ( D ) markers of exhaustion PD-1, PD-L1 and PD-L2 on NK cells in PDX derived PBMC, spleen and transplanted PDAC. ( E ) Frequency of conventional T cells (CD45 + CD56 − CD3 + ) and ( E ) expression of PD-1, PD-L1 and PD-L2 on conventional T cells, ( G ) frequencies of Th (CD45 + CD3 + CD4 + CD8 − ), and CTL (CD45 + CD3 + CD4 − CD8 + ) as percent of T cells, and ( H ) Treg (CD45 + CD3 + CD4 − CD8 + Foxp3 + ) as percent of Th cell subsets in PDX derived PBMC, spleen, and transplanted PDAC. Each data point represents one PDAC-SIS-PDX mouse tissue (n= 12-33 PDX mice per group, seven donor-cohorts). Data are represented as mean SEM; Paired t-test for donor-matched lung tissue vs. PDAC, one-way ANOVA with post-hoc Tukey test *p

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry, Expressing, Derivative Assay

    Immune cells in LUAD-SIS-PDX mice resemble exhausted Tumor Infiltrating Immune cells (TILs) cells of LUAD donors. ( A-H ) Flow cytometry of human immune cells from PBMC, spleen, and transplanted LUAD of PDX mice (“tumor”) analyzed 2-months post-transplant. ( A ) Frequency of human hematopoietic cells (CD45 + ), ( B ) NK cells (CD45 + CD3 − CD56 + ), and ( C ) expression of activating receptors CD16 and NKG2D on NK cells, and ( D ) PD-1, PD-L1 and PD-L2 on NK cells in PDX mouse derived PBMC, spleen and tumor. ( E ) Frequency of conventional T cells (CD45 + CD3 + CD56 − ), and ( F ) expression of PD-1, PD-L1 and PD-L2 on conventional T cells, as well as ( G ) frequencies of T helper (CD45 + CD3 + CD4 + CD8 − CD56 − ) and CTL CD45 + CD3 + CD4 − CD8 + CD56 − ), and ( H ) T-reg (CD45 + CD3 + CD4 + Foxp-3 + CD8 − CD56 − ) subsets in PDX mouse derived PBMC, spleen and tumor. (N = 10 - 23 PDX mice per group from five genetically unrelated human donor cohorts). Data are represented as mean SEM; one-way ANOVA with post-hoc Tukey test *p

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Immune cells in LUAD-SIS-PDX mice resemble exhausted Tumor Infiltrating Immune cells (TILs) cells of LUAD donors. ( A-H ) Flow cytometry of human immune cells from PBMC, spleen, and transplanted LUAD of PDX mice (“tumor”) analyzed 2-months post-transplant. ( A ) Frequency of human hematopoietic cells (CD45 + ), ( B ) NK cells (CD45 + CD3 − CD56 + ), and ( C ) expression of activating receptors CD16 and NKG2D on NK cells, and ( D ) PD-1, PD-L1 and PD-L2 on NK cells in PDX mouse derived PBMC, spleen and tumor. ( E ) Frequency of conventional T cells (CD45 + CD3 + CD56 − ), and ( F ) expression of PD-1, PD-L1 and PD-L2 on conventional T cells, as well as ( G ) frequencies of T helper (CD45 + CD3 + CD4 + CD8 − CD56 − ) and CTL CD45 + CD3 + CD4 − CD8 + CD56 − ), and ( H ) T-reg (CD45 + CD3 + CD4 + Foxp-3 + CD8 − CD56 − ) subsets in PDX mouse derived PBMC, spleen and tumor. (N = 10 - 23 PDX mice per group from five genetically unrelated human donor cohorts). Data are represented as mean SEM; one-way ANOVA with post-hoc Tukey test *p

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry, Expressing, Derivative Assay

    Immune cell reconstitution across a cohort of LUAD-SIS-PDX mice 4 months post engraftment. Flow cytometry of human immune cells from PBMC of single LUAD-PDX cohort of 15 mice analyzed 4-months post-transplant. Frequency of human hematopoietic cells (human CD45 + of total CD45 + ), NK cells (CD45 + CD56 + CD3 − ), NKT cells (CD45 + CD56 + CD3 + ), conventional T cells (CD45 + CD56 − CD3 + ), T helper cells (CD45 + CD56 − CD3 + CD4 + CD8 − ), and Cytotoxic T cells (CD45 + CD56 − CD3 + CD8 + CD4 − ). Each data point represents one LUAD-SIS-PDX mouse of a single human donor cohort, N=15 animals.

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Immune cell reconstitution across a cohort of LUAD-SIS-PDX mice 4 months post engraftment. Flow cytometry of human immune cells from PBMC of single LUAD-PDX cohort of 15 mice analyzed 4-months post-transplant. Frequency of human hematopoietic cells (human CD45 + of total CD45 + ), NK cells (CD45 + CD56 + CD3 − ), NKT cells (CD45 + CD56 + CD3 + ), conventional T cells (CD45 + CD56 − CD3 + ), T helper cells (CD45 + CD56 − CD3 + CD4 + CD8 − ), and Cytotoxic T cells (CD45 + CD56 − CD3 + CD8 + CD4 − ). Each data point represents one LUAD-SIS-PDX mouse of a single human donor cohort, N=15 animals.

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Flow Cytometry

    Human LUAD associated NK cells are exhausted in phenotype. Immune phenotyping of human NK cells in indicated tissues by flow cytometry. ( A, D ) Frequency of human NK cells (CD45 + CD3 − CD56 + ) in donor-matched lung vs. NSCLC (LUAD) tissue. ( B, E ) Frequency of CD16 and/or NKG2D expressing human NK cells, ( C, F ) Frequency of PD-1, PDL-1 and/or PDL-2-expressing human NK cells in donor-matched lung-tissue vs. LUAD ( A-C, top ), or LUAD-patient derived PBMC, lung tissue and LUAD ( D-F, bottom ). Each data point represents one genetically unrelated human donor (N= eight to 31 depending on tissue). Paired t-test for donor-matched lung tissue vs. LUAD, one-way ANOVA with post-hoc Tukey test for comparisons between LUAD-patient derived PBMC, lung tissue and LUAD (not always donor matched between all three tissues). *p

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Human LUAD associated NK cells are exhausted in phenotype. Immune phenotyping of human NK cells in indicated tissues by flow cytometry. ( A, D ) Frequency of human NK cells (CD45 + CD3 − CD56 + ) in donor-matched lung vs. NSCLC (LUAD) tissue. ( B, E ) Frequency of CD16 and/or NKG2D expressing human NK cells, ( C, F ) Frequency of PD-1, PDL-1 and/or PDL-2-expressing human NK cells in donor-matched lung-tissue vs. LUAD ( A-C, top ), or LUAD-patient derived PBMC, lung tissue and LUAD ( D-F, bottom ). Each data point represents one genetically unrelated human donor (N= eight to 31 depending on tissue). Paired t-test for donor-matched lung tissue vs. LUAD, one-way ANOVA with post-hoc Tukey test for comparisons between LUAD-patient derived PBMC, lung tissue and LUAD (not always donor matched between all three tissues). *p

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Flow Cytometry, Expressing, Derivative Assay

    Human PDAC associated NK and T cells are exhausted in phenotype. Frequency of ( A ) human hematopoietic cells (human CD45 + ), ( B ) NK cells (CD45 + CD56 + CD3 − ), ( C ) CD16 and/or NKG2D expressing human NK cells, ( D ) PD-1, PDL-1 and/or PDL-2-expressing human NK cells in in indicated freshly excised tissues of PDAC patients. Frequency of ( E ) human conventional T cells (CD45 + CD56 − CD3 + ), ( F ) PD-1, PDL-1 and/or PDL-2-expressing human T cells in PDAC-patient derived PBMC, pancreas and PDAC. Frequency of ( G ) Th (CD45 + CD3 + CD4 + CD8 − ) and CTL (CD45 + CD3 + CD4 − CD8 + ) as percent of human T cells, and ( H ) Treg (CD45 + CD3 + CD4 − CD8 + Foxp3 + ) as percent of Th cells in PDAC-patient derived PBMC, pancreas and PDAC. Each data point represents one genetically unrelated human donor. Paired t-test for donor-matched lung tissue vs. PDAC, one-way ANOVA with post-hoc Tukey test for comparisons between PDAC-patient derived PBMC, pancreas tissue and PDAC. *p

    Journal: bioRxiv

    Article Title: NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft

    doi: 10.1101/2020.05.24.112722

    Figure Lengend Snippet: Human PDAC associated NK and T cells are exhausted in phenotype. Frequency of ( A ) human hematopoietic cells (human CD45 + ), ( B ) NK cells (CD45 + CD56 + CD3 − ), ( C ) CD16 and/or NKG2D expressing human NK cells, ( D ) PD-1, PDL-1 and/or PDL-2-expressing human NK cells in in indicated freshly excised tissues of PDAC patients. Frequency of ( E ) human conventional T cells (CD45 + CD56 − CD3 + ), ( F ) PD-1, PDL-1 and/or PDL-2-expressing human T cells in PDAC-patient derived PBMC, pancreas and PDAC. Frequency of ( G ) Th (CD45 + CD3 + CD4 + CD8 − ) and CTL (CD45 + CD3 + CD4 − CD8 + ) as percent of human T cells, and ( H ) Treg (CD45 + CD3 + CD4 − CD8 + Foxp3 + ) as percent of Th cells in PDAC-patient derived PBMC, pancreas and PDAC. Each data point represents one genetically unrelated human donor. Paired t-test for donor-matched lung tissue vs. PDAC, one-way ANOVA with post-hoc Tukey test for comparisons between PDAC-patient derived PBMC, pancreas tissue and PDAC. *p

    Article Snippet: PBMC from LUAD and PDAC donors To isolate PBMC, peripheral blood was diluted 1:1 with Ca++ , Mg++ free phosphate buffered saline (PBS) and layered over Ficoll-Pacque (GE Healthcare), centrifuged at 2,000 rpm with no brake for 20 minutes at room temperature (RT) according to the manufacturer’s instructions.

    Techniques: Expressing, Derivative Assay

    Measurement of T cell proliferation using CFSE. The PBMCs were labeled with CFSE and then treated with PHA, PBS, pHSA and OsrHSA for 24, 48 and 72

    Journal: PLoS ONE

    Article Title: Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells

    doi: 10.1371/journal.pone.0104426

    Figure Lengend Snippet: Measurement of T cell proliferation using CFSE. The PBMCs were labeled with CFSE and then treated with PHA, PBS, pHSA and OsrHSA for 24, 48 and 72

    Article Snippet: PBMC preparation and isolation PBMCs were isolated from the plasma by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions.

    Techniques: Labeling

    The profiles of four cytokines in PBMCs following different treatments. The levels of cytokines were assayed using a CBA kit. Panels a, b, c and d present the data for the cytokines IFN-γ, TNF-α, IL-10 and IL-4, respectively. Each error bar with the same letter has the same significant level of p value.

    Journal: PLoS ONE

    Article Title: Immunotoxicity Assessment of Rice-Derived Recombinant Human Serum Albumin Using Human Peripheral Blood Mononuclear Cells

    doi: 10.1371/journal.pone.0104426

    Figure Lengend Snippet: The profiles of four cytokines in PBMCs following different treatments. The levels of cytokines were assayed using a CBA kit. Panels a, b, c and d present the data for the cytokines IFN-γ, TNF-α, IL-10 and IL-4, respectively. Each error bar with the same letter has the same significant level of p value.

    Article Snippet: PBMC preparation and isolation PBMCs were isolated from the plasma by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare, Uppsala, Sweden) according to the manufacturer’s instructions.

    Techniques: Crocin Bleaching Assay