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Becton Dickinson peripheral blood mononuclear cells pb mncs
Peripheral Blood Mononuclear Cells Pb Mncs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Incubation:

Article Title: Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation
Article Snippet: In parallel, PBMCs isolated by Ficoll-Hypaque density gradient centrifugation from same normal healthy donors, were pulsed with same concentration of HIV-VLPs for 12 hours. .. Analysis of DC phenotype MDDCs and PBMCs were incubated for 30 min at 4°C with murine monoclonal antibodies specific for CD80, CD83, CD86, HLA-DR and CD14 [PBMCs] (BD Pharmingen, San Diego, CA), washed and then fixed with 2% paraformaldehyde for analysis with a FACScalibur flow cytometer (BD Pharmingen). ..

Flow Cytometry:

Article Title: Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation
Article Snippet: In parallel, PBMCs isolated by Ficoll-Hypaque density gradient centrifugation from same normal healthy donors, were pulsed with same concentration of HIV-VLPs for 12 hours. .. Analysis of DC phenotype MDDCs and PBMCs were incubated for 30 min at 4°C with murine monoclonal antibodies specific for CD80, CD83, CD86, HLA-DR and CD14 [PBMCs] (BD Pharmingen, San Diego, CA), washed and then fixed with 2% paraformaldehyde for analysis with a FACScalibur flow cytometer (BD Pharmingen). ..

Cytometry:

Article Title: Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation
Article Snippet: In parallel, PBMCs isolated by Ficoll-Hypaque density gradient centrifugation from same normal healthy donors, were pulsed with same concentration of HIV-VLPs for 12 hours. .. Analysis of DC phenotype MDDCs and PBMCs were incubated for 30 min at 4°C with murine monoclonal antibodies specific for CD80, CD83, CD86, HLA-DR and CD14 [PBMCs] (BD Pharmingen, San Diego, CA), washed and then fixed with 2% paraformaldehyde for analysis with a FACScalibur flow cytometer (BD Pharmingen). ..

Isolation:

Article Title: Nitric Oxide Synthase Gene Transfer Restores Activity of Circulating Angiogenic Cells From Patients With Coronary Artery Disease
Article Snippet: .. Peripheral blood mononuclear cells (PB-MNCs) were isolated from 80 ml of blood by Ficoll gradient centrifugation (CPT Vacutainer Tubes; BD, Franklin Lakes, NJ) and plated at a density of 0.75 × 106 cells/cm2 on human fibronectin-coated dishes. .. Adherent cells were maintained in endothelial cell basal medium supplemented with 20% fetal bovine serum and several endothelial growth factors (EGM-2MV SingleQuots; Lonza, Basel, Switzerland), which was replaced every 48 hours.

Article Title: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes
Article Snippet: .. Isolation of pb-mncs from whole bloodPeripheral blood (10–15 mL) was drawn into a tube containing sodium heparin anticoagulant (BD Biosciences, Oxford, UK). .. PB-MNCs were purified using either Ficoll–Paque Premium (GE Healthcare, Uppsala, Sweden) or Lymphoprep (Stem Cell Technologies, Oslo, Norway).

Gradient Centrifugation:

Article Title: Nitric Oxide Synthase Gene Transfer Restores Activity of Circulating Angiogenic Cells From Patients With Coronary Artery Disease
Article Snippet: .. Peripheral blood mononuclear cells (PB-MNCs) were isolated from 80 ml of blood by Ficoll gradient centrifugation (CPT Vacutainer Tubes; BD, Franklin Lakes, NJ) and plated at a density of 0.75 × 106 cells/cm2 on human fibronectin-coated dishes. .. Adherent cells were maintained in endothelial cell basal medium supplemented with 20% fetal bovine serum and several endothelial growth factors (EGM-2MV SingleQuots; Lonza, Basel, Switzerland), which was replaced every 48 hours.

Cycling Probe Technology:

Article Title: Nitric Oxide Synthase Gene Transfer Restores Activity of Circulating Angiogenic Cells From Patients With Coronary Artery Disease
Article Snippet: .. Peripheral blood mononuclear cells (PB-MNCs) were isolated from 80 ml of blood by Ficoll gradient centrifugation (CPT Vacutainer Tubes; BD, Franklin Lakes, NJ) and plated at a density of 0.75 × 106 cells/cm2 on human fibronectin-coated dishes. .. Adherent cells were maintained in endothelial cell basal medium supplemented with 20% fetal bovine serum and several endothelial growth factors (EGM-2MV SingleQuots; Lonza, Basel, Switzerland), which was replaced every 48 hours.

Staining:

Article Title: Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma
Article Snippet: .. PB-MNCs were blocked with a rat anti-mouse CD16/32 antibody (BD Pharmingen) and stained with FITC conjugated anti-mouse CD45.2 (clone: 104; eBioscience), APC-eFluor780 conjugated anti-mouse CD45.1 (clone: A20; ebioscience), PE-conjugated anti-mouse B220 (clone: RA3-6B2; eBioscience), PE-Cy5 conjugated anti-mouse CD11b (clone: M1/70; eBioscience), PE-Cy5 conjugated anti-mouse Gr-1 (clone: RB6-8C5; eBioscience ) and Brilliant Violet 421 conjugated anti-mouse CD3e antibodies (clone: 145-2C11; BioLegend). .. Data were collected from at least 20,000 single cells by FACSCanto (BD Pharmingen) and analysed by FlowJo (Tree Star, Inc.) without knowledge of the genotype or treatment by a single observer (C.L.L.).

Article Title: WT1 PEPTIDE VACCINATION IN COMBINATION WITH IMATINIB THERAPY FOR A PATIENT WITH CML IN THE CHRONIC PHASE
Article Snippet: Identification of pDCs, mDC1s, γδT cells, Treg cells For identification of pDCs, mDC1s, γδT cells and Treg cells, PB-mononuclear cells (MNCs) were prepared from a WT1 peptide-treated patient and stained with FITC, PE or APC-labeled various monoclonal antibodies. pDCs were identified as CD303+ (BDCA2; Miltenyi Biotec, Bergisch Gladbach, Germany) cells. mDC1s were identified as lineage (CD3, CD14, CD19 and CD56)- / CD1c+ (BDCA1; Miltenyi Biotec) cells. γδT cells were identified as CD3+ /γδTCR+ cells. .. For identification of Treg cells, PB-MNCs were stained with surface molecules such as CD4 and CD25 (BD Biosciences). .. Then the cells were treated with freshly prepared Fixation/Permeabilization working solution (eBioscience, San Diego, CA) for 60 minutes and stained with PE-conjugated anti-human Foxp3 antibody (eBioscience) or isotype control.

Article Title: Quantitative Characterization of the T Cell Receptor Repertoire of Naïve and Memory Subsets Using an Integrated Experimental and Computational Pipeline Which Is Robust, Economical, and Versatile
Article Snippet: CD4 positive selection using Miltenyi beads was followed by CD8 positive selection of the CD4 negative fraction following the manufacturers’ instructions. .. CD4+ and CD8+ PBMC were stained with the following antibodies (all BD biosciences): CD3-PE-Cy7, CD4-V450, CD8-AF700, CD45RA-PE-CF594, and CD27-APC, as well as the fixable nearIR live/dead cell stain (Invitrogen). .. Cells were gated on lymphocytes, singlets, live cells, CD3+ and either CD4+ or CD8+ and then sorted into naïve T cells (CD45RA high, CD27+), central memory (CM, CD45RA−, CD27+), effector memory (EM, CD45RA−, CD27−) and effector memory RA-expressing revertants (EMRA, CD45RA+, CD27−) on an Aria II (BD).

Irradiation:

Article Title: Enhancement of the antigen-specific cytotoxic T lymphocyte-inducing ability in the PMDC11 leukemic plasmacytoid dendritic cell line via lentiviral vector-mediated transduction of the caTLR4 gene
Article Snippet: Briefly, PMDC11 cells or caTLR4-PMDC11 cells were irradiated with 30 Gy of 137 Cs generated gamma irradiation (PS-3000SB Cs-137; Pony Industry Co., Ltd., Osaka, Japan) immediately prior to MLC. .. A total of 1×105 allogeneic peripheral blood mononuclear cells (PB-MNCs) were co-cultured in a 96-well flat-bottom microtiter plate (BD Biosciences) with graded numbers (0, 1,250, 2,500 and 5,000) of irradiated PMDC11 cells or caTLR4-PMDC11 cells. .. The co-cultured cells were pulsed with 0.5 µ Ci (18.5 KBq)/well of [methyl-3 H]-thymidine (Perkin-Elmer, Inc., Waltham, MA, USA) on day five of culture and harvested with a cell harvester (Labo Mash; Futaba Medical, Inc., Tokyo, Japan) following overnight culture.

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    Becton Dickinson pbmcs
    Flow <t>cytometric</t> analyses of interferon gamma (IFN-γ) expression on NK cells and NK cell subsets in <t>PBMCs</t> isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of IFN-γ expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese individual. c Frequency of IFN-γ-expressing NK cells in PBMCs. d , e IFN-γ expression in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P
    Pbmcs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2021-03
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    86
    Becton Dickinson peripheral blood mononuclear cells pbmcs
    Irradiation-induced angiogenesis is dictated by MMP-9–mediated factor release from mast and stroma cells and incorporation of BM-derived cells. (A–C) HL ischemia was induced in Sl/Sl d and WBB6F1 +/+ mice, followed by 2 Gy IR or no IR ( n = 7). <t>PBMCs</t> were analyzed for the presence of CFU-Cs (A) and CFU-ECs (B). (C) Plasma VEGF was determined ( n = 7/group). (D) HL ischemia was induced in MMP-9 +/+ mice followed by 2 Gy IR. Mice were subdivided into groups receiving neutralizing <t>mAbs</t> against VEGFR-1, VEGFR-2 or a combination of both mAbs. (a) Blood flow was determined. Insert: Percentage of mice showing limb rescue after ligation. (b) Macroscopic pictures taken of ischemic and contralateral limb 7 d after ligation (*P
    Peripheral Blood Mononuclear Cells Pbmcs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmcs/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
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    86
    Becton Dickinson pbmc aliquots
    CXCR3 and CCR6 expression in T cells of major depressive disorder (MDD) patients and non-depressed controls. (A) CXCR3-expressing T cells were identified by flow cytometric analysis of peripheral blood mononuclear cells from MDD patients and matched non-depressed controls (CTR). Displayed values are frequencies of CXCR3 + T cells expressed as a percentage of live CD3 + lymphocytes from a representative case–control pair. (B) Percentages of CXCR3-expressing total T cells, <t>CD4</t> + , and CD8 + T cells were quantified in our cohort ( n = 40). (C,D) Similar analyses were conducted for the surface expression of CCR6 on total T cells as well as on the CD4 + and CD8 + T cell subsets. (E) The CXCR3 ligands CXCL10 and CXCL11 were quantified in sera of MDD patients and matched controls using a cytometric bead array ( n = 38). (F) Surface CD3 MFI levels were measured by flow cytometric analysis of CD4 + and CD8 + T cells from MDD patients and matched controls ( n = 40). All graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used. SSC-A, side scatter-area; CXCR3, CXC-chemokine receptor type 3; CCR6, CC-chemokine receptor type 6; MFI, median fluorescence intensity.
    Pbmc Aliquots, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson pbmc
    Signal scatter plots comparing the intensities of gene expressions on microarray chips within <t>Ficoll</t> and CPT-derived <t>PBMC</t> and their immune cell subsets for each healthy donor tested. The expression signals of Ficoll-derived cells were plotted against the expression signals of CPT-derived cells for each cell type and donor. A line of perfect correlation is indicated in all plots. Pearson’s correlation coefficients between each pair of samples are shown at the bottom right of each plot. The data showed that the pattern of gene expression was very similar between Ficoll and CPT-derived cell types, as indicated by the high correlation coefficients
    Pbmc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc - by Bioz Stars, 2021-03
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    Flow cytometric analyses of interferon gamma (IFN-γ) expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of IFN-γ expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese individual. c Frequency of IFN-γ-expressing NK cells in PBMCs. d , e IFN-γ expression in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Journal: Immunologic Research

    Article Title: Impaired natural killer cell subset phenotypes in human obesity

    doi: 10.1007/s12026-018-8989-4

    Figure Lengend Snippet: Flow cytometric analyses of interferon gamma (IFN-γ) expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of IFN-γ expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese individual. c Frequency of IFN-γ-expressing NK cells in PBMCs. d , e IFN-γ expression in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Article Snippet: For flow cytometric analyses, PBMCs were stained with the following antibodies (all from BD Biosciences, San Diego, USA, unless otherwise indicated): CD3 conjugated with phycoerythrin (PE)-Cy 7, CD4 conjugated with allophycocyanin (APC), CD 8 conjugated with PE, CD14 conjugated with fluorescein isothiocyanate (FITC), CD20 conjugated with APC-H7, CD56 conjugated with APC, CD253 (TRAIL; tumor necrosis factor-related apoptosis-inducing ligand) conjugated with PE, Ob-R (leptin receptor) conjugated with fluorescein (R & D Systems, Minneapolis, MN, USA), CD314 (NKG2D) conjugated with PE-CF594, CD25 conjugated with PE CF594, CD69 conjugated with FITC, and CD107a conjugated with PE.

    Techniques: Flow Cytometry, Expressing, Isolation, FACS

    Flow cytometric analyses of NK cells and NK cell subsets in PBMCs (peripheral blood mononuclear cells) isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of NK cells in PBMCs. d , e Expression of CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Journal: Immunologic Research

    Article Title: Impaired natural killer cell subset phenotypes in human obesity

    doi: 10.1007/s12026-018-8989-4

    Figure Lengend Snippet: Flow cytometric analyses of NK cells and NK cell subsets in PBMCs (peripheral blood mononuclear cells) isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of NK cells in PBMCs. d , e Expression of CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Article Snippet: For flow cytometric analyses, PBMCs were stained with the following antibodies (all from BD Biosciences, San Diego, USA, unless otherwise indicated): CD3 conjugated with phycoerythrin (PE)-Cy 7, CD4 conjugated with allophycocyanin (APC), CD 8 conjugated with PE, CD14 conjugated with fluorescein isothiocyanate (FITC), CD20 conjugated with APC-H7, CD56 conjugated with APC, CD253 (TRAIL; tumor necrosis factor-related apoptosis-inducing ligand) conjugated with PE, Ob-R (leptin receptor) conjugated with fluorescein (R & D Systems, Minneapolis, MN, USA), CD314 (NKG2D) conjugated with PE-CF594, CD25 conjugated with PE CF594, CD69 conjugated with FITC, and CD107a conjugated with PE.

    Techniques: Flow Cytometry, Isolation, FACS, Expressing

    Flow cytometric analyses of NKG2D receptor expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of NKG2D expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of NKG2D-positive NK cells in PBMCs. d , e Expression of NKG2D in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Journal: Immunologic Research

    Article Title: Impaired natural killer cell subset phenotypes in human obesity

    doi: 10.1007/s12026-018-8989-4

    Figure Lengend Snippet: Flow cytometric analyses of NKG2D receptor expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of NKG2D expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of NKG2D-positive NK cells in PBMCs. d , e Expression of NKG2D in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Article Snippet: For flow cytometric analyses, PBMCs were stained with the following antibodies (all from BD Biosciences, San Diego, USA, unless otherwise indicated): CD3 conjugated with phycoerythrin (PE)-Cy 7, CD4 conjugated with allophycocyanin (APC), CD 8 conjugated with PE, CD14 conjugated with fluorescein isothiocyanate (FITC), CD20 conjugated with APC-H7, CD56 conjugated with APC, CD253 (TRAIL; tumor necrosis factor-related apoptosis-inducing ligand) conjugated with PE, Ob-R (leptin receptor) conjugated with fluorescein (R & D Systems, Minneapolis, MN, USA), CD314 (NKG2D) conjugated with PE-CF594, CD25 conjugated with PE CF594, CD69 conjugated with FITC, and CD107a conjugated with PE.

    Techniques: Flow Cytometry, Expressing, Isolation, FACS

    Flow cytometric analyses of leptin receptor (Ob-R) expression in NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a Frequency of Ob-R expressing NK cells in PBMCs. b , c Expression of Ob-R in CD56 bright ( b ) and CD56 dim ( c ) NK cells. Data are expressed as mean ± SEM

    Journal: Immunologic Research

    Article Title: Impaired natural killer cell subset phenotypes in human obesity

    doi: 10.1007/s12026-018-8989-4

    Figure Lengend Snippet: Flow cytometric analyses of leptin receptor (Ob-R) expression in NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a Frequency of Ob-R expressing NK cells in PBMCs. b , c Expression of Ob-R in CD56 bright ( b ) and CD56 dim ( c ) NK cells. Data are expressed as mean ± SEM

    Article Snippet: For flow cytometric analyses, PBMCs were stained with the following antibodies (all from BD Biosciences, San Diego, USA, unless otherwise indicated): CD3 conjugated with phycoerythrin (PE)-Cy 7, CD4 conjugated with allophycocyanin (APC), CD 8 conjugated with PE, CD14 conjugated with fluorescein isothiocyanate (FITC), CD20 conjugated with APC-H7, CD56 conjugated with APC, CD253 (TRAIL; tumor necrosis factor-related apoptosis-inducing ligand) conjugated with PE, Ob-R (leptin receptor) conjugated with fluorescein (R & D Systems, Minneapolis, MN, USA), CD314 (NKG2D) conjugated with PE-CF594, CD25 conjugated with PE CF594, CD69 conjugated with FITC, and CD107a conjugated with PE.

    Techniques: Flow Cytometry, Expressing, Isolation

    Flow cytometric analyses of CD69 expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of CD69 expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of CD69-positive NK cells in PBMCs. d , e Expression of CD69 in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Journal: Immunologic Research

    Article Title: Impaired natural killer cell subset phenotypes in human obesity

    doi: 10.1007/s12026-018-8989-4

    Figure Lengend Snippet: Flow cytometric analyses of CD69 expression on NK cells and NK cell subsets in PBMCs isolated from normal-weight (nw) and obese (ob) individuals. a , b Exemplary FACS plots of CD69 expression in CD56 bright and CD56 dim NK cells of a normal-weight and an obese subject. c Frequency of CD69-positive NK cells in PBMCs. d , e Expression of CD69 in CD56 bright ( d ) and CD56 dim ( e ) NK cells. Data are expressed as mean ± SEM. * P

    Article Snippet: For flow cytometric analyses, PBMCs were stained with the following antibodies (all from BD Biosciences, San Diego, USA, unless otherwise indicated): CD3 conjugated with phycoerythrin (PE)-Cy 7, CD4 conjugated with allophycocyanin (APC), CD 8 conjugated with PE, CD14 conjugated with fluorescein isothiocyanate (FITC), CD20 conjugated with APC-H7, CD56 conjugated with APC, CD253 (TRAIL; tumor necrosis factor-related apoptosis-inducing ligand) conjugated with PE, Ob-R (leptin receptor) conjugated with fluorescein (R & D Systems, Minneapolis, MN, USA), CD314 (NKG2D) conjugated with PE-CF594, CD25 conjugated with PE CF594, CD69 conjugated with FITC, and CD107a conjugated with PE.

    Techniques: Flow Cytometry, Expressing, Isolation, FACS

    Irradiation-induced angiogenesis is dictated by MMP-9–mediated factor release from mast and stroma cells and incorporation of BM-derived cells. (A–C) HL ischemia was induced in Sl/Sl d and WBB6F1 +/+ mice, followed by 2 Gy IR or no IR ( n = 7). PBMCs were analyzed for the presence of CFU-Cs (A) and CFU-ECs (B). (C) Plasma VEGF was determined ( n = 7/group). (D) HL ischemia was induced in MMP-9 +/+ mice followed by 2 Gy IR. Mice were subdivided into groups receiving neutralizing mAbs against VEGFR-1, VEGFR-2 or a combination of both mAbs. (a) Blood flow was determined. Insert: Percentage of mice showing limb rescue after ligation. (b) Macroscopic pictures taken of ischemic and contralateral limb 7 d after ligation (*P

    Journal: The Journal of Experimental Medicine

    Article Title: Low-dose irradiation promotes tissue revascularization through VEGF release from mast cells and MMP-9-mediated progenitor cell mobilization

    doi: 10.1084/jem.20050959

    Figure Lengend Snippet: Irradiation-induced angiogenesis is dictated by MMP-9–mediated factor release from mast and stroma cells and incorporation of BM-derived cells. (A–C) HL ischemia was induced in Sl/Sl d and WBB6F1 +/+ mice, followed by 2 Gy IR or no IR ( n = 7). PBMCs were analyzed for the presence of CFU-Cs (A) and CFU-ECs (B). (C) Plasma VEGF was determined ( n = 7/group). (D) HL ischemia was induced in MMP-9 +/+ mice followed by 2 Gy IR. Mice were subdivided into groups receiving neutralizing mAbs against VEGFR-1, VEGFR-2 or a combination of both mAbs. (a) Blood flow was determined. Insert: Percentage of mice showing limb rescue after ligation. (b) Macroscopic pictures taken of ischemic and contralateral limb 7 d after ligation (*P

    Article Snippet: To understand which cell types increase after IR, we analyzed peripheral blood mononuclear cells (PBMCs) via FACS (Becton Dickinson) using mAbs against c-kit and VEGFR-2 after IR.

    Techniques: Irradiation, Derivative Assay, Mouse Assay, Flow Cytometry, Ligation

    CXCR3 and CCR6 expression in T cells of major depressive disorder (MDD) patients and non-depressed controls. (A) CXCR3-expressing T cells were identified by flow cytometric analysis of peripheral blood mononuclear cells from MDD patients and matched non-depressed controls (CTR). Displayed values are frequencies of CXCR3 + T cells expressed as a percentage of live CD3 + lymphocytes from a representative case–control pair. (B) Percentages of CXCR3-expressing total T cells, CD4 + , and CD8 + T cells were quantified in our cohort ( n = 40). (C,D) Similar analyses were conducted for the surface expression of CCR6 on total T cells as well as on the CD4 + and CD8 + T cell subsets. (E) The CXCR3 ligands CXCL10 and CXCL11 were quantified in sera of MDD patients and matched controls using a cytometric bead array ( n = 38). (F) Surface CD3 MFI levels were measured by flow cytometric analysis of CD4 + and CD8 + T cells from MDD patients and matched controls ( n = 40). All graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used. SSC-A, side scatter-area; CXCR3, CXC-chemokine receptor type 3; CCR6, CC-chemokine receptor type 6; MFI, median fluorescence intensity.

    Journal: Frontiers in Immunology

    Article Title: T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

    doi: 10.3389/fimmu.2018.00291

    Figure Lengend Snippet: CXCR3 and CCR6 expression in T cells of major depressive disorder (MDD) patients and non-depressed controls. (A) CXCR3-expressing T cells were identified by flow cytometric analysis of peripheral blood mononuclear cells from MDD patients and matched non-depressed controls (CTR). Displayed values are frequencies of CXCR3 + T cells expressed as a percentage of live CD3 + lymphocytes from a representative case–control pair. (B) Percentages of CXCR3-expressing total T cells, CD4 + , and CD8 + T cells were quantified in our cohort ( n = 40). (C,D) Similar analyses were conducted for the surface expression of CCR6 on total T cells as well as on the CD4 + and CD8 + T cell subsets. (E) The CXCR3 ligands CXCL10 and CXCL11 were quantified in sera of MDD patients and matched controls using a cytometric bead array ( n = 38). (F) Surface CD3 MFI levels were measured by flow cytometric analysis of CD4 + and CD8 + T cells from MDD patients and matched controls ( n = 40). All graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used. SSC-A, side scatter-area; CXCR3, CXC-chemokine receptor type 3; CCR6, CC-chemokine receptor type 6; MFI, median fluorescence intensity.

    Article Snippet: Cell Purification For qRT-PCR and TCR sequencing analyses, CD4+ T cells were purified using magnetic beads (negative selection by BD IMag Human CD4 T Lymphocyte Enrichment Set, BD Biosciences) from PBMC aliquots thawed in cell separation buffer (1% human serum, 2 mM EDTA in PBS).

    Techniques: Expressing, Flow Cytometry, Fluorescence

    Surface and intracellular staining of CXCR3. (A) Percentages of CXCR3- and CCR6-expressing CD3 − lymphocytes (non-T cells) were quantified in major depressive disorder (MDD) patients and matched non-depressed controls (CTR) ( n = 40). (B) A representative plot shows fluorescence intensity of CXCR3 expression in intact (surface CXCR3; light gray-shaded curve) relative to fixed and permeabilized T cells (total cellular CXCR3; dark gray-shaded curve). Isotype-matched negative controls were used at the same concentration before fixation (black-dashed curve) and after fixation-permeabilization (gray-dashed curve) and showed no positive staining for CXCR3. (C) Total cellular CXCR3 MFI levels were measured by flow cytometric analysis of fixed and permeabilized peripheral blood mononuclear cells (PBMCs) from MDD patients and matched controls ( n = 36). Stained PBMCs were gated on live CD3 + lymphocytes (T cells), CD4 + and CD8 + T cell subsets as well as CD3 − lymphocytes (non-T cells). Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used. CXCR3: CXC-chemokine receptor type 3; CCR6: CC-chemokine receptor type 6; MFI: median fluorescence intensity.

    Journal: Frontiers in Immunology

    Article Title: T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

    doi: 10.3389/fimmu.2018.00291

    Figure Lengend Snippet: Surface and intracellular staining of CXCR3. (A) Percentages of CXCR3- and CCR6-expressing CD3 − lymphocytes (non-T cells) were quantified in major depressive disorder (MDD) patients and matched non-depressed controls (CTR) ( n = 40). (B) A representative plot shows fluorescence intensity of CXCR3 expression in intact (surface CXCR3; light gray-shaded curve) relative to fixed and permeabilized T cells (total cellular CXCR3; dark gray-shaded curve). Isotype-matched negative controls were used at the same concentration before fixation (black-dashed curve) and after fixation-permeabilization (gray-dashed curve) and showed no positive staining for CXCR3. (C) Total cellular CXCR3 MFI levels were measured by flow cytometric analysis of fixed and permeabilized peripheral blood mononuclear cells (PBMCs) from MDD patients and matched controls ( n = 36). Stained PBMCs were gated on live CD3 + lymphocytes (T cells), CD4 + and CD8 + T cell subsets as well as CD3 − lymphocytes (non-T cells). Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used. CXCR3: CXC-chemokine receptor type 3; CCR6: CC-chemokine receptor type 6; MFI: median fluorescence intensity.

    Article Snippet: Cell Purification For qRT-PCR and TCR sequencing analyses, CD4+ T cells were purified using magnetic beads (negative selection by BD IMag Human CD4 T Lymphocyte Enrichment Set, BD Biosciences) from PBMC aliquots thawed in cell separation buffer (1% human serum, 2 mM EDTA in PBS).

    Techniques: Staining, Expressing, Fluorescence, Concentration Assay, Flow Cytometry

    Regulatory T cells in major depressive disorder (MDD) patients and non-depressed controls. (A) Regulatory T cells (Tregs) were identified by flow cytometric analysis of peripheral blood mononuclear cells from MDD patients and matched non-depressed controls (CTR). Displayed values are frequencies of Tregs expressed as a percentage of live CD4 + T cells from a representative case–control pair. (B) Differences in Treg frequency are depicted for the entire cohort ( n = 40). (C) Negatively selected CD4 + T cells from a subsample of patients and matched controls ( n = 20) were analyzed for mRNA expression of the T helper-associated transcription factors Forkhead box P3 ( FOXP3 ), T-box 21 ( T-bet ), GATA binding protein 3 ( GATA3 ), and RAR related orphan receptor C ( RORC ), respectively. Expression was normalized to the geometric mean expression of three housekeeping genes ( IPO8, TBP, RPL13A ). (D) The correlation between the expression levels of the gene FOXP3 in purified CD4 + T cells and the frequency of Tregs expressed as a percentage of CD4 + T cells is plotted ( n = 20). Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used.

    Journal: Frontiers in Immunology

    Article Title: T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

    doi: 10.3389/fimmu.2018.00291

    Figure Lengend Snippet: Regulatory T cells in major depressive disorder (MDD) patients and non-depressed controls. (A) Regulatory T cells (Tregs) were identified by flow cytometric analysis of peripheral blood mononuclear cells from MDD patients and matched non-depressed controls (CTR). Displayed values are frequencies of Tregs expressed as a percentage of live CD4 + T cells from a representative case–control pair. (B) Differences in Treg frequency are depicted for the entire cohort ( n = 40). (C) Negatively selected CD4 + T cells from a subsample of patients and matched controls ( n = 20) were analyzed for mRNA expression of the T helper-associated transcription factors Forkhead box P3 ( FOXP3 ), T-box 21 ( T-bet ), GATA binding protein 3 ( GATA3 ), and RAR related orphan receptor C ( RORC ), respectively. Expression was normalized to the geometric mean expression of three housekeeping genes ( IPO8, TBP, RPL13A ). (D) The correlation between the expression levels of the gene FOXP3 in purified CD4 + T cells and the frequency of Tregs expressed as a percentage of CD4 + T cells is plotted ( n = 20). Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used.

    Article Snippet: Cell Purification For qRT-PCR and TCR sequencing analyses, CD4+ T cells were purified using magnetic beads (negative selection by BD IMag Human CD4 T Lymphocyte Enrichment Set, BD Biosciences) from PBMC aliquots thawed in cell separation buffer (1% human serum, 2 mM EDTA in PBS).

    Techniques: Flow Cytometry, Expressing, Binding Assay, Purification

    Peripheral blood counts and frequencies of major leukocyte subsets. (A) Absolute peripheral blood granulocyte, monocyte, and lymphocyte counts were obtained from major depressive disorder (MDD) patients and matched non-depressed controls (CTR) using a Coulter Ac·T Diff hematology analyzer ( n = 40). (B) Frequencies of total CD3 + lymphocytes (T cells), CD3 − CD56 − CD19 + B cells and CD3 − CD19 − CD20 − CD14 − CD56 + natural killer (NK) cells were obtained by flow cytometric analysis of thawed peripheral blood mononuclear cells. (C) T cells were further discriminated into CD4 + CD8 − and CD8 + CD4 − subsets. (D) Among NK cells, CD56 low CD16 + cytotoxic (NKc) cells and CD56 high CD16 − regulatory (NKreg) cells were also identified. Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used.

    Journal: Frontiers in Immunology

    Article Title: T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

    doi: 10.3389/fimmu.2018.00291

    Figure Lengend Snippet: Peripheral blood counts and frequencies of major leukocyte subsets. (A) Absolute peripheral blood granulocyte, monocyte, and lymphocyte counts were obtained from major depressive disorder (MDD) patients and matched non-depressed controls (CTR) using a Coulter Ac·T Diff hematology analyzer ( n = 40). (B) Frequencies of total CD3 + lymphocytes (T cells), CD3 − CD56 − CD19 + B cells and CD3 − CD19 − CD20 − CD14 − CD56 + natural killer (NK) cells were obtained by flow cytometric analysis of thawed peripheral blood mononuclear cells. (C) T cells were further discriminated into CD4 + CD8 − and CD8 + CD4 − subsets. (D) Among NK cells, CD56 low CD16 + cytotoxic (NKc) cells and CD56 high CD16 − regulatory (NKreg) cells were also identified. Graphs depict medians with interquartile ranges. For all comparisons, the Wilcoxon signed-rank test was used.

    Article Snippet: Cell Purification For qRT-PCR and TCR sequencing analyses, CD4+ T cells were purified using magnetic beads (negative selection by BD IMag Human CD4 T Lymphocyte Enrichment Set, BD Biosciences) from PBMC aliquots thawed in cell separation buffer (1% human serum, 2 mM EDTA in PBS).

    Techniques: Flow Cytometry

    Signal scatter plots comparing the intensities of gene expressions on microarray chips within Ficoll and CPT-derived PBMC and their immune cell subsets for each healthy donor tested. The expression signals of Ficoll-derived cells were plotted against the expression signals of CPT-derived cells for each cell type and donor. A line of perfect correlation is indicated in all plots. Pearson’s correlation coefficients between each pair of samples are shown at the bottom right of each plot. The data showed that the pattern of gene expression was very similar between Ficoll and CPT-derived cell types, as indicated by the high correlation coefficients

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Signal scatter plots comparing the intensities of gene expressions on microarray chips within Ficoll and CPT-derived PBMC and their immune cell subsets for each healthy donor tested. The expression signals of Ficoll-derived cells were plotted against the expression signals of CPT-derived cells for each cell type and donor. A line of perfect correlation is indicated in all plots. Pearson’s correlation coefficients between each pair of samples are shown at the bottom right of each plot. The data showed that the pattern of gene expression was very similar between Ficoll and CPT-derived cell types, as indicated by the high correlation coefficients

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Microarray, Cycling Probe Technology, Derivative Assay, Expressing

    Yield and viability of immune cell subsets prepared from PBMC isolated by either Ficoll or CPT protocol. a The mean number of CD19+, CD8+, CD14+, and CD4+ cells positively selected from Ficoll- and CPT-isolated PBMC collected from 6 healthy donors. The horizontal line denotes the means and each symbol represents an individual cell type isolated from Ficoll-PBMC (filled symbols) or CPT-PBMC (empty symbols) from each of 6 donors. b The mean viability of the same cell subsets. No significant differences ( P

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Yield and viability of immune cell subsets prepared from PBMC isolated by either Ficoll or CPT protocol. a The mean number of CD19+, CD8+, CD14+, and CD4+ cells positively selected from Ficoll- and CPT-isolated PBMC collected from 6 healthy donors. The horizontal line denotes the means and each symbol represents an individual cell type isolated from Ficoll-PBMC (filled symbols) or CPT-PBMC (empty symbols) from each of 6 donors. b The mean viability of the same cell subsets. No significant differences ( P

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Cycling Probe Technology

    Principle component analysis of gene expression showing distinct clusters between individual immune cell subsets but not between the same subsets derived from PBMC isolated by CPT or Ficoll protocols. Principle component analysis (PCA) was performed based on expression of all genes from the microarray. Individual immune cell types are represented by different colors: CD19+ B cells, blue; CD8+ T cells, green; CD14+ monocytes, purple; CD4+ T cells, orange; and total PBMC, red. The method used for PBMC isolation is represented by different symbols of differing size: CPT-based procedure by large circles and Ficoll-based procedure by small circles. PC#1, principle component #1; PC#2, principle component #2

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Principle component analysis of gene expression showing distinct clusters between individual immune cell subsets but not between the same subsets derived from PBMC isolated by CPT or Ficoll protocols. Principle component analysis (PCA) was performed based on expression of all genes from the microarray. Individual immune cell types are represented by different colors: CD19+ B cells, blue; CD8+ T cells, green; CD14+ monocytes, purple; CD4+ T cells, orange; and total PBMC, red. The method used for PBMC isolation is represented by different symbols of differing size: CPT-based procedure by large circles and Ficoll-based procedure by small circles. PC#1, principle component #1; PC#2, principle component #2

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Expressing, Derivative Assay, Isolation, Cycling Probe Technology, Microarray

    Yield and quality of RNA recovered from total PBMC and their individual immune cell subsets did not significantly differ between PBMC isolated by Ficoll and CPT methods. a The mean amount of RNA per cell and ( b ) the mean RIN of RNA preparations extracted from total PBMC and their individual immune cell subsets derived from PBMC prepared by Ficoll and CPT methods from 6 health donors. There were no significant differences ( P

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Yield and quality of RNA recovered from total PBMC and their individual immune cell subsets did not significantly differ between PBMC isolated by Ficoll and CPT methods. a The mean amount of RNA per cell and ( b ) the mean RIN of RNA preparations extracted from total PBMC and their individual immune cell subsets derived from PBMC prepared by Ficoll and CPT methods from 6 health donors. There were no significant differences ( P

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Cycling Probe Technology, Derivative Assay

    Similar quantities of DNA with comparable quality were obtained from PBMC and immune cell subsets following either Ficoll or CPT protocol of PBMC isolation. a The mean amount of DNA extracted per cell and ( b ) the mean of the 260/280 ratios, as measure of DNA quality, were compared between DNA preparations obtained from total PBMC and immune cell subsets prepared from PBMC isolated by Ficoll and CPT methods from 6 healthy donors. There were no significant differences ( P

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Similar quantities of DNA with comparable quality were obtained from PBMC and immune cell subsets following either Ficoll or CPT protocol of PBMC isolation. a The mean amount of DNA extracted per cell and ( b ) the mean of the 260/280 ratios, as measure of DNA quality, were compared between DNA preparations obtained from total PBMC and immune cell subsets prepared from PBMC isolated by Ficoll and CPT methods from 6 healthy donors. There were no significant differences ( P

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Cycling Probe Technology, Isolation

    Schematic outline of the study. PBMC from 6 healthy donors were isolated using Ficoll-Paque gradient fractionation or BD Vacutainer CPT protocol, and cell yield and purity were compared. Subsequently, immune cell subsets were separated by positive selection from PBMC obtained by both methods, and yields and viabilities of the subsets were determined and compared. RNA and DNA were extracted from total PBMC isolated by both protocols and from their subsets, and the nucleic acid yield and quality compared. Finally, gene expression analysis of individual immune cell subsets and total PBMC obtained by Ficoll and CPT isolation methods were performed and the results compared. LN 2 , liquid nitrogen

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Schematic outline of the study. PBMC from 6 healthy donors were isolated using Ficoll-Paque gradient fractionation or BD Vacutainer CPT protocol, and cell yield and purity were compared. Subsequently, immune cell subsets were separated by positive selection from PBMC obtained by both methods, and yields and viabilities of the subsets were determined and compared. RNA and DNA were extracted from total PBMC isolated by both protocols and from their subsets, and the nucleic acid yield and quality compared. Finally, gene expression analysis of individual immune cell subsets and total PBMC obtained by Ficoll and CPT isolation methods were performed and the results compared. LN 2 , liquid nitrogen

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Fractionation, Cycling Probe Technology, Selection, Expressing

    Yield and viability of PBMC isolated by the Ficoll and CPT protocols. a The mean numbers of PBMC per ml of blood obtained by Ficoll or CPT isolation procedure from 6 healthy donors. b The mean viability of PBMC freshly isolated from the same 6 healthy donors by either Ficoll gradient separation or CPT technique. No significant differences ( P

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Yield and viability of PBMC isolated by the Ficoll and CPT protocols. a The mean numbers of PBMC per ml of blood obtained by Ficoll or CPT isolation procedure from 6 healthy donors. b The mean viability of PBMC freshly isolated from the same 6 healthy donors by either Ficoll gradient separation or CPT technique. No significant differences ( P

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Cycling Probe Technology

    Purity of immune cell subsets obtained from PBMC isolated by either Ficoll or CPT procedures. CD19+, CD8+, CD14+, and CD4+ cell subsets were sequentially separated from PBMC isolated by Ficoll and CPT protocols from the same donor and their purity was determined by flow cytometry using antibodies against surface markers specific for individual immune cell types. Filled histograms were given by appropriate immunoglobulin isotype controls. Gates for determining positivity were established using isotype controls so that ~99.0 % of events were negative

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Purity of immune cell subsets obtained from PBMC isolated by either Ficoll or CPT procedures. CD19+, CD8+, CD14+, and CD4+ cell subsets were sequentially separated from PBMC isolated by Ficoll and CPT protocols from the same donor and their purity was determined by flow cytometry using antibodies against surface markers specific for individual immune cell types. Filled histograms were given by appropriate immunoglobulin isotype controls. Gates for determining positivity were established using isotype controls so that ~99.0 % of events were negative

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Cycling Probe Technology, Flow Cytometry, Cytometry

    Post-cryopreservation recovery and viability of PBMC isolated by the Ficoll and CPT methods. a The mean percent recovery after cryopreservation of PBMC collected using the Ficoll and CPT protocols. Percent recovery was calculated by dividing the number of PBMC recovered after thawing by the number of cells that were cryopreserved. b The mean viability of recovered PBMC isolated by the Ficoll and CPT technique. No significant differences ( P

    Journal: BMC Immunology

    Article Title: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

    doi: 10.1186/s12865-015-0113-0

    Figure Lengend Snippet: Post-cryopreservation recovery and viability of PBMC isolated by the Ficoll and CPT methods. a The mean percent recovery after cryopreservation of PBMC collected using the Ficoll and CPT protocols. Percent recovery was calculated by dividing the number of PBMC recovered after thawing by the number of cells that were cryopreserved. b The mean viability of recovered PBMC isolated by the Ficoll and CPT technique. No significant differences ( P

    Article Snippet: About 45 ml of blood was obtained from each donor using BD Vacutainer tubes containing acid-citrate-dextrose anticoagulant, solution A (ACD-A; BD) from which PBMC were isolated by Ficoll-Paque gradient centrifugation (see below).

    Techniques: Isolation, Cycling Probe Technology