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Ficoll-Paque Pharmacia peripheral blood mononuclear cell pbmc
Semi-quantitative <t>PCR</t> analysis of BLV pol detects differences in proviral load between BLV SGV and BLV-rabbits. <t>PBMC</t> were harvested at month indicated after challenge with 1 × 10 8 FLK/BLV cells from indicated rabbits that had been infected with BLV SGV for 24 months or from age-matched naïve controls. DNA from 50,000 PMBC was subjected to nested PCR and gel electrophoresis and stained with ethidium bromide. The position of the 591-bp amplicon is designated by the labeled arrow. In parallel, DNA from 20,000 PBMC was subjected to PCR with primers complementary to β- actin . The position of the 594-bp amplicon is designated by the labeled arrow and the signals were quantified by densitometry.
Peripheral Blood Mononuclear Cell Pbmc, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Long-term infection with retroviral structural gene vector provides protection against bovine leukemia virus disease in rabbits"

Article Title: Long-term infection with retroviral structural gene vector provides protection against bovine leukemia virus disease in rabbits

Journal: Virology

doi: 10.1016/j.virol.2004.09.001

Semi-quantitative PCR analysis of BLV pol detects differences in proviral load between BLV SGV and BLV-rabbits. PBMC were harvested at month indicated after challenge with 1 × 10 8 FLK/BLV cells from indicated rabbits that had been infected with BLV SGV for 24 months or from age-matched naïve controls. DNA from 50,000 PMBC was subjected to nested PCR and gel electrophoresis and stained with ethidium bromide. The position of the 591-bp amplicon is designated by the labeled arrow. In parallel, DNA from 20,000 PBMC was subjected to PCR with primers complementary to β- actin . The position of the 594-bp amplicon is designated by the labeled arrow and the signals were quantified by densitometry.
Figure Legend Snippet: Semi-quantitative PCR analysis of BLV pol detects differences in proviral load between BLV SGV and BLV-rabbits. PBMC were harvested at month indicated after challenge with 1 × 10 8 FLK/BLV cells from indicated rabbits that had been infected with BLV SGV for 24 months or from age-matched naïve controls. DNA from 50,000 PMBC was subjected to nested PCR and gel electrophoresis and stained with ethidium bromide. The position of the 591-bp amplicon is designated by the labeled arrow. In parallel, DNA from 20,000 PBMC was subjected to PCR with primers complementary to β- actin . The position of the 594-bp amplicon is designated by the labeled arrow and the signals were quantified by densitometry.

Techniques Used: Real-time Polymerase Chain Reaction, Infection, Nested PCR, Nucleic Acid Electrophoresis, Staining, Amplification, Labeling, Polymerase Chain Reaction

Related Articles

Centrifugation:

Article Title: Contribution of CD8+ T Cells to Gamma Interferon Production in Human Tuberculosis
Article Snippet: .. PBMC were obtained by centrifugation over Ficoll-Paque (Pharmacia, Uppsala, Sweden). .. CD4+ or CD8+ cells were isolated from PBMC, using positive selection with magnetic beads conjugated to anti-CD4 or anti-CD8 (Miltenyi Biotech, Auburn, Calif.) ( ).

Article Title: HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
Article Snippet: Blood (7.5 ml) was collected in preservative free heparin (final concentration of 100 iu/ml) and cells were prepared immediately. .. PBMC were isolated by density centrifugation using Ficoll Paque (Pharmacia Biotech, Sweden). ..

Article Title: Host's innate immune response to fungal and bacterial agents in vitro: up-regulation of interleukin-15 gene expression resulting in enhanced natural killer cell activity
Article Snippet: .. PBMC from healthy donors were prepared (after their informed and written consent, following the approval of our research by this Center's Ethics Committee) by centrifugation of heparinized venous blood over Ficoll-paque (Pharmacia, Montreal, Canada) gradients and collected as previously described. .. The separated cells were washed and cultured in complete medium: RPMI-1640 culture medium (Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% glutamine, 100 U/ml penicillin, 20 µg/ml streptomycin, and 1 µg/ml gentamicin.

Article Title: Early Therapy of Vertical Human Immunodeficiency Virus Type 1 (HIV-1) Infection: Control of Viral Replication and Absence of Persistent HIV-1-Specific Immune Responses
Article Snippet: Following repeat centrifugation of plasma at 1,500 × g for 10 min at room temperature, the supernatant was removed, divided into aliquots of 0.5 ml, and frozen promptly at −70°C. .. PBMC were recovered from the cell layer by Ficoll-Paque (Pharmacia, Piscataway, N.J.) density centrifugation ( ). .. HIV-1 RNA was quantified in 200 μl of EDTA-anticoagulated plasma (stored at −70°C within 6 h following phlebotomy) by PCR after reverse transcription (Amplicor; Roche).

Article Title: New live attenuated tuberculosis vaccine MTBVAC induces trained immunity and confers protection against experimental lethal pneumonia
Article Snippet: .. Peripheral blood mononuclear cell and monocyte isolation PBMC isolation was performed by differential density centrifugation over Ficoll-Paque (GE Healthcare). .. Percoll isolation of monocytes was performed as previously described [ ].

Isolation:

Article Title: HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
Article Snippet: Blood (7.5 ml) was collected in preservative free heparin (final concentration of 100 iu/ml) and cells were prepared immediately. .. PBMC were isolated by density centrifugation using Ficoll Paque (Pharmacia Biotech, Sweden). ..

Article Title: Epstein-Barr Virus Coinfection in Children Boosts Cytomegalovirus-Induced Differentiation of Natural Killer Cells
Article Snippet: This study was approved by the Human Ethics Committee at Karolinska University Hospital (Huddinge, Sweden [Dnr 331/02]) and the Karolinska Institute, and all of the parents provided their informed consent. .. PBMC were isolated by density gradient centrifugation (Ficoll-Paque; Pharmacia Diagnostics AB, Sweden) and cryopreserved in liquid nitrogen. .. For flow cytometric analyses, the PBMC were thawed, washed three times, and resuspended in culture medium (RPMI 1640 supplemented with 10% heat-inactivated fetal calf serum [HyClone Laboratories, Inc., USA]), l -glutamine (2 mmol/liter), penicillin G sodium (100 U/ml), and streptomycin sulfate (100 μg/ml; Merck, Darmstadt, Germany) to a concentration of 106 cells/ml and cultured at 37°C and 5% CO2 .

Article Title: New live attenuated tuberculosis vaccine MTBVAC induces trained immunity and confers protection against experimental lethal pneumonia
Article Snippet: .. Peripheral blood mononuclear cell and monocyte isolation PBMC isolation was performed by differential density centrifugation over Ficoll-Paque (GE Healthcare). .. Percoll isolation of monocytes was performed as previously described [ ].

Gradient Centrifugation:

Article Title: Epstein-Barr Virus Coinfection in Children Boosts Cytomegalovirus-Induced Differentiation of Natural Killer Cells
Article Snippet: This study was approved by the Human Ethics Committee at Karolinska University Hospital (Huddinge, Sweden [Dnr 331/02]) and the Karolinska Institute, and all of the parents provided their informed consent. .. PBMC were isolated by density gradient centrifugation (Ficoll-Paque; Pharmacia Diagnostics AB, Sweden) and cryopreserved in liquid nitrogen. .. For flow cytometric analyses, the PBMC were thawed, washed three times, and resuspended in culture medium (RPMI 1640 supplemented with 10% heat-inactivated fetal calf serum [HyClone Laboratories, Inc., USA]), l -glutamine (2 mmol/liter), penicillin G sodium (100 U/ml), and streptomycin sulfate (100 μg/ml; Merck, Darmstadt, Germany) to a concentration of 106 cells/ml and cultured at 37°C and 5% CO2 .

Article Title: Activation of Fc?R1 on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses
Article Snippet: .. PBMC were purified by Ficoll-Hypaque gradient centrifugation (Ficoll-Paque; Pharmacia Biotech AB). .. Peripheral blood monocytes were isolated from PBMC thought Percoll (Amersham Biosciences, Uppsala, Sweden) density gradient centrifugation technique.

Article Title: In vitro treatment of human transforming growth factor-?1-treated monocyte-derived dendritic cells with haptens can induce the phenotypic and functional changes similar to epidermal Langerhans cells in the initiation phase of allergic contact sensitivity reaction
Article Snippet: The levels of IL-1β, TNF-α, IL-10, IL-12p70, or MMP-9 were calculated by using a standard curve obtained with recombinant IL-1β (from 0 to 125 pg/ml), recombinant TNF-α (from 0·5 to 32·0 pg/ml), recombinant IL-10 (from 0 to 256 pg/ml), recombinant IL-12p70 (from 0 to 600 pg/ml), and recombinant MMP-9 (from 0 to 64 ng/ml). .. PBMC were obtained by Ficoll–Paque (Pharmacia) gradient centrifugation of heparinized blood. .. These PBMC were treated with a pan-T-cell isolation kit (Miltenyi Biotec), according to the manufacturer's protocol.

Purification:

Article Title: Activation of Fc?R1 on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses
Article Snippet: .. PBMC were purified by Ficoll-Hypaque gradient centrifugation (Ficoll-Paque; Pharmacia Biotech AB). .. Peripheral blood monocytes were isolated from PBMC thought Percoll (Amersham Biosciences, Uppsala, Sweden) density gradient centrifugation technique.

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    Ficoll-Paque Pharmacia pbmcs
    Semiquantitative <t>PCR</t> analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in <t>PBMC</t> from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.
    Pbmcs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2021-03
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    86
    Ficoll-Paque Pharmacia pbmc preparation method
    Trima-associated gene expression signatures (A) Identification of gene expression patterns across all <t>PBMC</t> cell-types (left) linked to method of PBMC isolation (e.g., <t>Ficoll</t> or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.
    Pbmc Preparation Method, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc preparation method/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc preparation method - by Bioz Stars, 2021-03
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    86
    Ficoll-Paque Pharmacia hla class i typing peripheral blood mononuclear cells pbmcs
    <t>HLA-HIV</t> associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA <t>class</t> I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p
    Hla Class I Typing Peripheral Blood Mononuclear Cells Pbmcs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla class i typing peripheral blood mononuclear cells pbmcs/product/Ficoll-Paque Pharmacia
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    Price from $9.99 to $1999.99
    hla class i typing peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
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    Semiquantitative PCR analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in PBMC from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.

    Journal: Journal of Virology

    Article Title: Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model

    doi:

    Figure Lengend Snippet: Semiquantitative PCR analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in PBMC from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.

    Article Snippet: To prepare PBMCs samples for PCR, PBMCs were isolated by Ficoll-Paque (Pharmacia) gradient centrifugation and washed three times with phosphate-buffered saline.

    Techniques: Polymerase Chain Reaction, Labeling, Positive Control, Marker, Amplification

    Trima-associated gene expression signatures (A) Identification of gene expression patterns across all PBMC cell-types (left) linked to method of PBMC isolation (e.g., Ficoll or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.

    Journal: bioRxiv

    Article Title: No detectable alloreactive transcriptional responses during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

    doi: 10.1101/2020.02.12.946509

    Figure Lengend Snippet: Trima-associated gene expression signatures (A) Identification of gene expression patterns across all PBMC cell-types (left) linked to method of PBMC isolation (e.g., Ficoll or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.

    Article Snippet: Moreover, our results demonstrate how PBMC preparation method (e.g., Ficoll-Paque density gradient centrifugation with or without Trima filtration) and sample multiplexing technology (e.g., SCMK) can introduce confounding variables into scRNA-seq data.

    Techniques: Expressing, Isolation, Marker

    Exploring alloreactivity in CD4+ T-cell subset and classical monocytes (A) Marker genes used for CD4+ T-cell subtype annotations (left) in Ficoll PBMCs. n = 6,879 CD4+ T-cells. (B) Evenly-subsetted classical monocyte gene expression space grouped according to donor ID (e.g., A, B, C) and mixing status (e.g., - = unmixed, + = mixed). n = 384 classical monocytes. (C) Quantitative inter-sample comparison using Jensen-Shannon Divergence (JSD) and hierarchical clustering demonstrates similarity between evenly-subsetted classical monocytes from distinct healthy donors regardless of mixing. (D) Expression of genes known to be up-regulated (e.g., IFNG and CD40LG) or down-regulated (e.g., DUSP1 and FOS) in lymphocytes during an allogenic response.

    Journal: bioRxiv

    Article Title: No detectable alloreactive transcriptional responses during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

    doi: 10.1101/2020.02.12.946509

    Figure Lengend Snippet: Exploring alloreactivity in CD4+ T-cell subset and classical monocytes (A) Marker genes used for CD4+ T-cell subtype annotations (left) in Ficoll PBMCs. n = 6,879 CD4+ T-cells. (B) Evenly-subsetted classical monocyte gene expression space grouped according to donor ID (e.g., A, B, C) and mixing status (e.g., - = unmixed, + = mixed). n = 384 classical monocytes. (C) Quantitative inter-sample comparison using Jensen-Shannon Divergence (JSD) and hierarchical clustering demonstrates similarity between evenly-subsetted classical monocytes from distinct healthy donors regardless of mixing. (D) Expression of genes known to be up-regulated (e.g., IFNG and CD40LG) or down-regulated (e.g., DUSP1 and FOS) in lymphocytes during an allogenic response.

    Article Snippet: Moreover, our results demonstrate how PBMC preparation method (e.g., Ficoll-Paque density gradient centrifugation with or without Trima filtration) and sample multiplexing technology (e.g., SCMK) can introduce confounding variables into scRNA-seq data.

    Techniques: Marker, Expressing

    HLA-HIV associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA class I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: HLA-HIV associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA class I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Expressing, Infection, Chick Chorioallantoic Membrane Assay, MANN-WHITNEY

    HLA class I haplotype structures and linkage disequilibrium in the MEX (panel A) and CAM (panel B) cohorts. HLA loci are stacked vertically, with each orange tile representing a specific HLA subtype, and with segments connecting linked alleles on adjacent loci. The height of each tile and the thickness of each segment correspond to HLA allele and haplotype frequencies, respectively. The most frequent HLA allele pairs (two-loci) found to be in linkage disequilibrium are highlighted in green (PF > 0.10) and blue (PF

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: HLA class I haplotype structures and linkage disequilibrium in the MEX (panel A) and CAM (panel B) cohorts. HLA loci are stacked vertically, with each orange tile representing a specific HLA subtype, and with segments connecting linked alleles on adjacent loci. The height of each tile and the thickness of each segment correspond to HLA allele and haplotype frequencies, respectively. The most frequent HLA allele pairs (two-loci) found to be in linkage disequilibrium are highlighted in green (PF > 0.10) and blue (PF

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Chick Chorioallantoic Membrane Assay

    Comparison of HLA class I allele frequencies between the Mestizo MEX/CAM cohort (n = 3213) and the mainly Caucasian HOMER cohort (n = 1622). Allele frequencies (2n) were calculated using the HLA Analysis tool from Los Alamos HIV Database ( https://www.hiv.lanl.gov ); all HLA AF > 0.001 in at least one cohort are shown here. AF were compared using Fisher’s exact test, with multiple tests addressed using q-values 42 . Significant differences (p

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: Comparison of HLA class I allele frequencies between the Mestizo MEX/CAM cohort (n = 3213) and the mainly Caucasian HOMER cohort (n = 1622). Allele frequencies (2n) were calculated using the HLA Analysis tool from Los Alamos HIV Database ( https://www.hiv.lanl.gov ); all HLA AF > 0.001 in at least one cohort are shown here. AF were compared using Fisher’s exact test, with multiple tests addressed using q-values 42 . Significant differences (p

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Chick Chorioallantoic Membrane Assay