perfect lipids transfection kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher perfect lipids transfection kit
    Inhibition of NK function by transient <t>transfection</t> with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid ( WT ) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51 Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was
    Perfect Lipids Transfection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perfect lipids transfection kit/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    perfect lipids transfection kit - by Bioz Stars, 2020-07
    80/100 stars

    Images

    1) Product Images from "Control of Lytic Function by Mitogen-activated Protein Kinase/Extracellular Regulatory Kinase 2 (ERK2) in a Human Natural Killer Cell Line: Identification of Perforin and Granzyme B Mobilization by Functional ERK2 "

    Article Title: Control of Lytic Function by Mitogen-activated Protein Kinase/Extracellular Regulatory Kinase 2 (ERK2) in a Human Natural Killer Cell Line: Identification of Perforin and Granzyme B Mobilization by Functional ERK2

    Journal: The Journal of Experimental Medicine

    doi:

    Inhibition of NK function by transient transfection with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid ( WT ) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51 Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was
    Figure Legend Snippet: Inhibition of NK function by transient transfection with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid ( WT ) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51 Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was

    Techniques Used: Inhibition, Transfection, Dominant Negative Mutation, Construct, Mutagenesis, Plasmid Preparation, Lysis, Labeling

    Related Articles

    Transfection:

    Article Title: Control of Lytic Function by Mitogen-activated Protein Kinase/Extracellular Regulatory Kinase 2 (ERK2) in a Human Natural Killer Cell Line: Identification of Perforin and Granzyme B Mobilization by Functional ERK2
    Article Snippet: .. YT cells were transfected using the PerFect Lipids transfection kit (Invitrogen Corp., San Diego, CA), and transfection efficiency in YT cells was first optimized using the control plasmid pcDNA3.1/ His/lacZ (GAL) included in the kit. .. Using β-galactosidase expression from the control GAL plasmid in YT cells, it was determined that 4 μg/ml of plasmid DNA provided 35–40% transfection with the least toxicity.

    Plasmid Preparation:

    Article Title: Control of Lytic Function by Mitogen-activated Protein Kinase/Extracellular Regulatory Kinase 2 (ERK2) in a Human Natural Killer Cell Line: Identification of Perforin and Granzyme B Mobilization by Functional ERK2
    Article Snippet: .. YT cells were transfected using the PerFect Lipids transfection kit (Invitrogen Corp., San Diego, CA), and transfection efficiency in YT cells was first optimized using the control plasmid pcDNA3.1/ His/lacZ (GAL) included in the kit. .. Using β-galactosidase expression from the control GAL plasmid in YT cells, it was determined that 4 μg/ml of plasmid DNA provided 35–40% transfection with the least toxicity.

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  • 85
    Thermo Fisher lipid based transient transfection
    Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and <t>transfections.</t>
    Lipid Based Transient Transfection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transient transfection/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid based transient transfection - by Bioz Stars, 2020-07
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    Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and transfections.

    Journal: ACS chemical biology

    Article Title: Switching Cyclic Nucleotide-Selective Activation of Cyclic Adenosine Monophosphate-Dependent Protein Kinase Holoenzyme Reveals Distinct Roles of Tandem Cyclic Nucleotide-Binding Domains

    doi: 10.1021/acschembio.7b00732

    Figure Lengend Snippet: Measurements of PKA activation using the AKAR FRET biosensor for RI α −/− cells expressing the various RI α constructs. (A, B) Examples of RI α −/− cells expressing the indicated constructs stimulated with 8-CPT-cAMP or 8-CPT-cGMP at t = 0 (minutes). Cell images as shown were pseudocolored according to the calculated YFP/CFP FRET ratios, with the range displayed indicated on the color bars at right. Left panels show the tracings of the FRET ratios as % change over time. Scale bars: 10 μ M. (C) Box plots of the data showing all 8-CPT-cAMP and 8-CPT-cGMP cell stimulations plotted as % change of the normalized FRET ratios. Total number of individual cells analyzed for each condition is between 20 and 26, encompassing multiple independently conducted stimulations and transfections.

    Article Snippet: In additional experiments, lipid-based transient transfection was carried out using Lipofectamine 2000 (Thermo-Fisher).

    Techniques: Activation Assay, Expressing, Construct, Cycling Probe Technology, Transfection