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Biochrom percoll solution
Percoll Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/percoll solution/product/Biochrom
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
percoll solution - by Bioz Stars, 2020-09
92/100 stars

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Modification:

Article Title: Electrophysiological properties of human mesenchymal stem cells
Article Snippet: .. Briefly, an aliquot from bone marrow aspirate diluted with PBS–0.5% human serum albumin (HSA) was layered over a Percoll solution (d = 1.073 g ml−1 , Biochrom, Germany) and centrifuged at 900 g for 30 min. Mononuclear cells at the interface were recovered, washed twice in PBS–HSA and seeded into 75 cm2 flasks containing Dulbecco's modified Eagle's medium (DMEM, low glucose) supplemented with 2 m m GlutaMAX, 10 U ml−1 penicillin, 100 μg ml−1 streptomycin (all from Gibco Invitrogen, UK) and 10% fetal calf serum (Biochrom). ..

Centrifugation:

Article Title: Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli
Article Snippet: .. Cells were collected by centrifugation (at 250 × g for 8 min), resuspended in 25 ml of Percoll solution (density adjusted to 1.0500 g/ml with PBS-EDTA [PBS supplemented with 5.4 mM EDTA]; Biochrom, Berlin, Germany), and layered onto 10 ml of Percoll solution (density adjusted to 1.0816 g/ml) in a 50-ml centrifugation tube. ..

other:

Article Title: Fluorescence based cell counting in collagen monolayer cultures of primary hepatocytes
Article Snippet: Briefly, 20 ml of a 50 % Percoll solution [Easycoll, Biochrom, Berlin, Germany; diluted with phosphate buffered saline (PBS)] were overlaid with the cell suspension (max.

Article Title: Spheroid culture of mesenchymal stromal cells results in morpho-rheological properties appropriate for improved microcirculation
Article Snippet: A 20 ml aliquot was layered over a 1,073 g/ml Percoll solution (Biochrom, Berlin, Germany) and centrifuged at 2000 × g for 15 min at room temperature.

Article Title: The Cell Surface Proteome of Human Mesenchymal Stromal Cells
Article Snippet: A 20-ml aliquot was layered over a Percoll solution (d = 1.073 g/ml; Biochrom, Berlin, Germany) and centrifuged at 900 g for 30 min at room temperature.

Article Title: Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation
Article Snippet: A 20 mL aliquot was layered over a 1073 g mL−1 Percoll solution (Biochrom, Berlin, Germany) and centrifuged at 2000×g for 15 min at room temperature.

Gradient Centrifugation:

Article Title: AIDeveloper: deep learning image classification in life science and beyond
Article Snippet: .. In brief, BM aspirates were diluted in phosphate buffered saline (PBS, Sigma-Aldrich, Germany) at a ratio of 1:2 and a density gradient centrifugation (2,000 g for 15 min at room temperature) using a 20 mL aliquot layered over a 1.073 g/mL Percoll solution (Biochrom, Berlin, Germany). .. The fraction of mononucleated cells (MNCs) were transferred to a cell culture flask in MSC medium.

Article Title: AIDeveloper: deep learning image classification in life science and beyond
Article Snippet: .. Blood aspirates were diluted in PBS at a ratio of 1:5, followed by a density gradient centrifugation (2000 g for 15 min at room temperature) using a 20 mL aliquot layered over a 1,073 g/mL Percoll solution (Biochrom, Berlin, Germany). .. The MNC fraction was washed once in PBS and 100 μL aliquots were used to stain B-cells using an antibody against CD19 (coupled to allophycocyanin; APC), T-cells using anti-CD3 (coupled to fluorescein; FITC) and NK-cells, as a subset of T-cells using anti-CD56 (coupled to phycoerythrin; PE).

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  • 85
    Biochrom percoll percoll reagent
    Separation of viable and dead hepatocytes by density gradient centrifugation. a A 25 % <t>Percoll</t> solution is carefully overlaid with the suspension containing a maximum of 50 million viable cells. b After the centrifugation, the dead cells and the cell debris are located at the interphase, while viable cells are pelleted
    Percoll Percoll Reagent, supplied by Biochrom, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/percoll percoll reagent/product/Biochrom
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    percoll percoll reagent - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    92
    Biochrom percoll solution
    Separation of viable and dead hepatocytes by density gradient centrifugation. a A 25 % <t>Percoll</t> solution is carefully overlaid with the suspension containing a maximum of 50 million viable cells. b After the centrifugation, the dead cells and the cell debris are located at the interphase, while viable cells are pelleted
    Percoll Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/percoll solution/product/Biochrom
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    percoll solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Separation of viable and dead hepatocytes by density gradient centrifugation. a A 25 % Percoll solution is carefully overlaid with the suspension containing a maximum of 50 million viable cells. b After the centrifugation, the dead cells and the cell debris are located at the interphase, while viable cells are pelleted

    Journal: Archives of Toxicology

    Article Title: Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME

    doi: 10.1007/s00204-013-1078-5

    Figure Lengend Snippet: Separation of viable and dead hepatocytes by density gradient centrifugation. a A 25 % Percoll solution is carefully overlaid with the suspension containing a maximum of 50 million viable cells. b After the centrifugation, the dead cells and the cell debris are located at the interphase, while viable cells are pelleted

    Article Snippet: Hepatocyte purification with Percoll® Percoll® reagent (Easycoll Separating Solution, desity 1.124 g/ml, Biochrom AG, Berlin, Germany, Cat. No. L6143) is a useful tool for further purification of the isolated rat or mouse hepatocytes.

    Techniques: Gradient Centrifugation, Centrifugation