peptide synthesizer  (Gyros Protein Technologies)


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    Name:
    TRIBUTE 230 VAC 50 60 Hz
    Description:
    Tribute Automated 2 Channel Solid Phase Peptide Synthesizer 230 VAC 50 60 Hz Complete with embedded computer Tribute software touch screen monitor and cables PTI Peptide Predictor software safety shield 20 Liter single waste container safety coated solvent bottles 3 x 1 L 2 x 4 L 12 collect tubes Standard one year warranty
    Catalog Number:
    TPS-230
    Price:
    None
    Category:
    Instruments
    Applications:
    Peptide Synthesis, Organic Synthesis
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    Structured Review

    Gyros Protein Technologies peptide synthesizer
    Tribute Automated 2 Channel Solid Phase Peptide Synthesizer 230 VAC 50 60 Hz Complete with embedded computer Tribute software touch screen monitor and cables PTI Peptide Predictor software safety shield 20 Liter single waste container safety coated solvent bottles 3 x 1 L 2 x 4 L 12 collect tubes Standard one year warranty
    https://www.bioz.com/result/peptide synthesizer/product/Gyros Protein Technologies
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peptide synthesizer - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Synthesized:

    Article Title: Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
    Article Snippet: Hydrogel moduli were verified with rheometry and atomic force microscopy as described previously , where moduli of hydrogels (0.25 mm thick) in phosphate buffered saline (PBS) were measured as E ∼32 kPa and ∼7 kPa after 0 minute and 5 minutes of irradiation (365 nm at 10 mW/cm2 ), respectively. .. Peptide Synthesis An integrin-binding adhesion peptide was synthesized and incorporated within the cell culture platform to promote cell attachment based on established protocols , Briefly, OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was made on a solid phase peptide synthesizer (Tribute, Protein Technologies, Inc.) with HBTU/HOBt amino acid activation and Fmoc chemistry . .. After synthesis of the primary sequence, the N-terminus of the peptide was modified on resin with an acryloyl group by reaction with HATU-activated acrylic acid in the presence of diisopropylethylamine (4 molar excess of each relative to N-terminus amine) .

    Article Title: Efficient refolding and functional characterization of PfAMA1(DI+DII) expressed in E. coli
    Article Snippet: The refolded protein was then concentrated and loaded onto a HiLoad 16/600 Superdex 200 pg column (Cytiva –GE healthcare Life Sciences, USA) equilibrated with buffer F (20 mM Tris, pH 7.8 and 100 mM NaCl) to remove the soluble aggregates if any. .. 2.5 Chemical synthesis of PfRON2ed 39-residue PfRON2ed peptide (Asp2021-Ser2059) was synthesized by step-wise Fmoc chemistry solid phase peptide synthesis (SPPS) in an automated peptide synthesizer (Tribute UV-IR, Protein Technologies Inc. USA). .. The peptide was synthesized on 2-chlorotrityl chloride (CTC) resin.

    Article Title: Tuning microenvironment modulus and biochemical composition promotes human mesenchymal stem cell tenogenic differentiation
    Article Snippet: Product purity was confirmed by 1 H-NMR in DMSO-d6: 400 mHz δ 6.20-5.86 (m, 2H), δ 3.65-3.40 (m, 227H) and disappearance of the peak at δ 3.10-2.90 (t, 2H) ( ). .. The peptides CGKGWGKGCG (peptide crosslinker), CGRGDS (RGDS), C(POG)4 FOGERG(POG)4 G (collagen mimetic peptide [CMP]), and CGRGSD (scrambled peptide [RGSD]) were synthesized using standard Fmoc-chemistry on a peptide synthesizer (Tribute, Protein Technologies, Inc., Tucson, AZ). ..

    Article Title: Bioinspired Histidine–Zn2+ Coordination for Tuning the Mechanical Properties of Self-Healing Coiled Coil Cross-Linked Hydrogels
    Article Snippet: Most interestingly, we demonstrate that this additional cross-linking and self-healing mode allows for reversibly and dynamically tuning the viscoelastic properties (i.e., the relaxation time) of the hydrogel as a function of the Zn2+ concentration. .. Peptide Synthesis and Purification The CC-forming peptides were synthesized using solid-phase peptide synthesis on a Tribute peptide synthesizer from Gyros Protein Technologies AB (Uppsala, Sweden). .. All amino acids were acquired from Bachem AG (Bubendorf, Switzerland) and 2-(6-chlor-1H -benzotriazol-1-yl)-1,1,3,3-tetramethylaminium-hexafluorophosphate (HCTU) was purchased from Novabiochem® (Merck KGaA, Darmstadt, Germany).

    Article Title: Photoresponsive elastic properties of azobenzene-containing poly(ethylene-glycol)-based hydrogels
    Article Snippet: 3,4’-Fmoc-AMPB ( ): 1 H NMR (DMSO- d6 , 500 MHz): δ= 4.26 (t, 1H), 4.29 (d, 2H), 4.40 (d, 2H), 7.35 (t, 2H), 7.43 (m, 4H), 7.73 (m, 3H), 7.90 (d, 2H), 7.91 (d, 2H), 7.99 (t, 1H), 8.14 (m, 2H), 8.39 (s, 1H). .. An azobenzene-containing peptide crosslinker (KCGGPQG-3,4’-AMPB-IWGQGCK) was synthesized on a solid phase peptide synthesizer (Tribute Protein Synthesizer, Protein Technologies, Inc.) using Rink Amide MBHA resin (Novabiochem, 0.59 mmol/g). ..

    Article Title: A peptide extension dictates IgM assembly
    Article Snippet: .. All peptides were synthesized manually or on an automated synthesizer (Tribute; Gyros Protein Technologies) by Fmoc-based, solid-phase peptide synthesis using 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) activation in N , N -dimethylformamide (DMF) on Wang resins ( ). .. Double coupling and pseudoproline dipeptide building blocks (Iris Biotech) were used to improve synthesis quality as indicated in .

    Cell Culture:

    Article Title: Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
    Article Snippet: Hydrogel moduli were verified with rheometry and atomic force microscopy as described previously , where moduli of hydrogels (0.25 mm thick) in phosphate buffered saline (PBS) were measured as E ∼32 kPa and ∼7 kPa after 0 minute and 5 minutes of irradiation (365 nm at 10 mW/cm2 ), respectively. .. Peptide Synthesis An integrin-binding adhesion peptide was synthesized and incorporated within the cell culture platform to promote cell attachment based on established protocols , Briefly, OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was made on a solid phase peptide synthesizer (Tribute, Protein Technologies, Inc.) with HBTU/HOBt amino acid activation and Fmoc chemistry . .. After synthesis of the primary sequence, the N-terminus of the peptide was modified on resin with an acryloyl group by reaction with HATU-activated acrylic acid in the presence of diisopropylethylamine (4 molar excess of each relative to N-terminus amine) .

    Cell Attachment Assay:

    Article Title: Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
    Article Snippet: Hydrogel moduli were verified with rheometry and atomic force microscopy as described previously , where moduli of hydrogels (0.25 mm thick) in phosphate buffered saline (PBS) were measured as E ∼32 kPa and ∼7 kPa after 0 minute and 5 minutes of irradiation (365 nm at 10 mW/cm2 ), respectively. .. Peptide Synthesis An integrin-binding adhesion peptide was synthesized and incorporated within the cell culture platform to promote cell attachment based on established protocols , Briefly, OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was made on a solid phase peptide synthesizer (Tribute, Protein Technologies, Inc.) with HBTU/HOBt amino acid activation and Fmoc chemistry . .. After synthesis of the primary sequence, the N-terminus of the peptide was modified on resin with an acryloyl group by reaction with HATU-activated acrylic acid in the presence of diisopropylethylamine (4 molar excess of each relative to N-terminus amine) .

    Activation Assay:

    Article Title: Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus
    Article Snippet: Hydrogel moduli were verified with rheometry and atomic force microscopy as described previously , where moduli of hydrogels (0.25 mm thick) in phosphate buffered saline (PBS) were measured as E ∼32 kPa and ∼7 kPa after 0 minute and 5 minutes of irradiation (365 nm at 10 mW/cm2 ), respectively. .. Peptide Synthesis An integrin-binding adhesion peptide was synthesized and incorporated within the cell culture platform to promote cell attachment based on established protocols , Briefly, OOGRGDSG (diethylene glycol-diethylene glycol-glycine-arginine-glycine-aspartic acid-serine-glycine) was made on a solid phase peptide synthesizer (Tribute, Protein Technologies, Inc.) with HBTU/HOBt amino acid activation and Fmoc chemistry . .. After synthesis of the primary sequence, the N-terminus of the peptide was modified on resin with an acryloyl group by reaction with HATU-activated acrylic acid in the presence of diisopropylethylamine (4 molar excess of each relative to N-terminus amine) .

    Article Title: A peptide extension dictates IgM assembly
    Article Snippet: .. All peptides were synthesized manually or on an automated synthesizer (Tribute; Gyros Protein Technologies) by Fmoc-based, solid-phase peptide synthesis using 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) activation in N , N -dimethylformamide (DMF) on Wang resins ( ). .. Double coupling and pseudoproline dipeptide building blocks (Iris Biotech) were used to improve synthesis quality as indicated in .

    Purification:

    Article Title: Bioinspired Histidine–Zn2+ Coordination for Tuning the Mechanical Properties of Self-Healing Coiled Coil Cross-Linked Hydrogels
    Article Snippet: Most interestingly, we demonstrate that this additional cross-linking and self-healing mode allows for reversibly and dynamically tuning the viscoelastic properties (i.e., the relaxation time) of the hydrogel as a function of the Zn2+ concentration. .. Peptide Synthesis and Purification The CC-forming peptides were synthesized using solid-phase peptide synthesis on a Tribute peptide synthesizer from Gyros Protein Technologies AB (Uppsala, Sweden). .. All amino acids were acquired from Bachem AG (Bubendorf, Switzerland) and 2-(6-chlor-1H -benzotriazol-1-yl)-1,1,3,3-tetramethylaminium-hexafluorophosphate (HCTU) was purchased from Novabiochem® (Merck KGaA, Darmstadt, Germany).

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    Gyros Protein Technologies tnfr1 blocking peptide
    Effects of PGRN on hepatic autophagy and insulin sensitivity via the <t>TNFR1-dependent</t> manner in vivo.
    Tnfr1 Blocking Peptide, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnfr1 blocking peptide/product/Gyros Protein Technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnfr1 blocking peptide - by Bioz Stars, 2021-06
    86/100 stars
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    92
    Gyros Protein Technologies automatic peptide synthesizer
    Effects of PGRN on hepatic autophagy and insulin sensitivity via the <t>TNFR1-dependent</t> manner in vivo.
    Automatic Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/automatic peptide synthesizer/product/Gyros Protein Technologies
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    automatic peptide synthesizer - by Bioz Stars, 2021-06
    92/100 stars
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    80
    Gyros Protein Technologies ps3 automated peptide synthesizer
    Effects of PGRN on hepatic autophagy and insulin sensitivity via the <t>TNFR1-dependent</t> manner in vivo.
    Ps3 Automated Peptide Synthesizer, supplied by Gyros Protein Technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ps3 automated peptide synthesizer/product/Gyros Protein Technologies
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ps3 automated peptide synthesizer - by Bioz Stars, 2021-06
    80/100 stars
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    Image Search Results


    Effects of PGRN on hepatic autophagy and insulin sensitivity via the TNFR1-dependent manner in vivo.

    Journal: Molecular Endocrinology

    Article Title: PGRN Induces Impaired Insulin Sensitivity and Defective Autophagy in Hepatic Insulin Resistance

    doi: 10.1210/me.2014-1266

    Figure Lengend Snippet: Effects of PGRN on hepatic autophagy and insulin sensitivity via the TNFR1-dependent manner in vivo.

    Article Snippet: The DNA sequence encoding the TNFR1 blocking peptide was directly synthesized by Protein technologies Inc.

    Techniques: In Vivo

    Blockade of TNFR1 or knockdown TNFR1 on autophagy and insulin signaling in hepatocytes.

    Journal: Molecular Endocrinology

    Article Title: PGRN Induces Impaired Insulin Sensitivity and Defective Autophagy in Hepatic Insulin Resistance

    doi: 10.1210/me.2014-1266

    Figure Lengend Snippet: Blockade of TNFR1 or knockdown TNFR1 on autophagy and insulin signaling in hepatocytes.

    Article Snippet: The DNA sequence encoding the TNFR1 blocking peptide was directly synthesized by Protein technologies Inc.

    Techniques: