pepmap 100 c 18 column  (Thermo Fisher)


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    Name:
    PepMap 100 C18 LC Columns
    Description:
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    Catalog Number:
    164571ts
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Chromatography Columns Resins Spin Filters
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    Structured Review

    Thermo Fisher pepmap 100 c 18 column
    Generate high resolution analyses of tryptic natural and synthetic peptides using Thermo Scientific Acclaim PepMap 100 C18 LC Columns These columns are often used for LC MS MS peptide mapping for protein identification biomarker discovery and systems biology Due to their high loading capacity Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples Most of these C18 columns are pre assembled with Thermo Scientific nanoViper Fingertight Fittings for easy installation Classic columns are available as well
    https://www.bioz.com/result/pepmap 100 c 18 column/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pepmap 100 c 18 column - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Low T3 State Is Correlated with Cardiac Mitochondrial Impairments after Ischemia Reperfusion Injury: Evidence from a Proteomic Approach
    Article Snippet: .. The loading pump pre-concentrated the sample in a pre-column cartridge (PepMap-100 C18 5 µm 100 A, 0.1 × 20 mm, Thermo Scientific, Waltham, MA, USA) and then separated in a C18 PepMap-100 column (3 µm, 75 µm × 250 mm, Thermo Scientific) at a flow rate of 300 nL·min−1 . ..

    Liquid Chromatography:

    Article Title: Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors
    Article Snippet: .. Liquid chromatography was performed on a fully automated Ultimate 3000 RSLC nano System (Thermo Scientific) fitted with a 100 µm x 2 cm PepMap100 C18 nano trap column and a 75 μm × 25 cm reverse phase C18 nano column (Aclaim PepMap, Thermo Scientific). ..

    Article Title: The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module
    Article Snippet: .. Mass Spectrometry Data Acquisition Liquid chromatography was performed on a fully automated Ultimate U3000 Nano LC System (Dionex) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm reverse phase PepMap100 C18 nano column (Dionex). ..

    Mass Spectrometry:

    Article Title: The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module
    Article Snippet: .. Mass Spectrometry Data Acquisition Liquid chromatography was performed on a fully automated Ultimate U3000 Nano LC System (Dionex) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm reverse phase PepMap100 C18 nano column (Dionex). ..

    Chromatography:

    Article Title: Hydroxyl Radical Dosimetry for High Flux Hydroxyl Radical Protein Footprinting Applications Using a Simple Optical Detection Method
    Article Snippet: .. Chromatography was conducted using a 150×0.075 mm PepMap 100 C18 analytical column with 3 μm particle size (Thermo Fisher Scientific, Waltham, MA, USA). .. The gradient elution was performed from 4% to 40% acetonitrile in 0.1% formic acid over 15 min at a flow rate of 0.3 μL/min for angiotensin; a 4% to 50% acetonitrile in 0.1% formic acid over 50 min at a flow rate of 0.3 μL/min for lysozyme, and then increased to 90% acetonitrile in 0.1% formic acid for 5 min followed by a 15 min re-equilibration step.

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  • 99
    Thermo Fisher c18 pre column
    Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via <t>C18-RP-LC-ESI-Q-TOF</t> tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black
    C18 Pre Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 pre column/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c18 pre column - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher c18 analytical column
    Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via <t>C18-RP-LC-ESI-Q-TOF</t> tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black
    C18 Analytical Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 analytical column/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    c18 analytical column - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via C18-RP-LC-ESI-Q-TOF tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics

    doi: 10.1007/s13361-015-1308-6

    Figure Lengend Snippet: Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via C18-RP-LC-ESI-Q-TOF tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black

    Article Snippet: Tryptic and synthetic peptide (500 fmol) mixtures, dissolved in 0.1% FA, were trapped on a C18 pre-column (Acclaim PepMap RSLC Nano-Trap column; 3 μm, 100 Å, 75 μm × 20 mm, Thermo Fisher Scientific, Waltham, MA, USA) and separated on a C18 analytical column (Acclaim PepMap RSLC column; 2 μm, 100 Å, 75 μm × 150 mm, Thermo Fisher Scientific) using a linear gradient from 2% buffer B (100% ACN, 0.1% FA) to 50% in 30 min, with buffer A containing 0.1% FA.

    Techniques: Derivative Assay, Mass Spectrometry

    Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via C18-RP-LC-ESI-Q-TOF tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics

    doi: 10.1007/s13361-015-1308-6

    Figure Lengend Snippet: Correlation between precursor m/z and optimal collision energies. Synthetic glycopeptides (diamonds) were spiked into a mixture of glycopeptides (circles) enriched from a tryptic digest derived from a complex sample and analyzed via C18-RP-LC-ESI-Q-TOF tandem MS. For the [M + 5H] 5+ species and partially for the [M + 4H] 4+ species of the synthetic glycopeptides, values were obtained additionally by direct infusion. The optimal collision energies for peptide backbone and glycan moiety were determined based on GlycoQuest Score and peptide intensity coverage. [M + 5H] 5+ species are indicated in orange, [M + 4H] 4+ in blue, [M + 3H] 3+ in green, and [M + 2H] 2+ in black

    Article Snippet: Tryptic and synthetic peptide (500 fmol) mixtures, dissolved in 0.1% FA, were trapped on a C18 pre-column (Acclaim PepMap RSLC Nano-Trap column; 3 μm, 100 Å, 75 μm × 20 mm, Thermo Fisher Scientific, Waltham, MA, USA) and separated on a C18 analytical column (Acclaim PepMap RSLC column; 2 μm, 100 Å, 75 μm × 150 mm, Thermo Fisher Scientific) using a linear gradient from 2% buffer B (100% ACN, 0.1% FA) to 50% in 30 min, with buffer A containing 0.1% FA.

    Techniques: Derivative Assay, Mass Spectrometry

    C18-PGC-LC-ESI-QTOF-MS/MS analysis of Pronase-generated O -glycopeptides with multiple glycosylation sites after PNGase F N -glycan release and exoglycosidase treatment with sialidase and galactosidase. A , Extracted ion chromatograms of three different glycopeptide clusters spanning from Thr79 to Ser121 with different glycan compositions attached. Pep1 = 79 TANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ; Pep2 = 82 TTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ; Pep3 = 81 D TTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ( D indicates the deamidated Asn caused by PNGase F treatment) B , Stepping-energy CID spectrum of Pep3 with 14 HexNAc residues, indicating up to 14 occupied O -glycosylation sites. C , Stepping-energy CID spectrum of the same glycopeptide as in panel B using lower-energy stepping-energy CID (stepping energy is set to 60 and 80% each half of the time (instead of 80–140%) with a focus on the glycan-derived Y-ions.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: N- and O-glycosylation Analysis of Human C1-inhibitor Reveals Extensive Mucin-type O-Glycosylation *

    doi: 10.1074/mcp.RA117.000240

    Figure Lengend Snippet: C18-PGC-LC-ESI-QTOF-MS/MS analysis of Pronase-generated O -glycopeptides with multiple glycosylation sites after PNGase F N -glycan release and exoglycosidase treatment with sialidase and galactosidase. A , Extracted ion chromatograms of three different glycopeptide clusters spanning from Thr79 to Ser121 with different glycan compositions attached. Pep1 = 79 TANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ; Pep2 = 82 TTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ; Pep3 = 81 D TTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGS 121 ( D indicates the deamidated Asn caused by PNGase F treatment) B , Stepping-energy CID spectrum of Pep3 with 14 HexNAc residues, indicating up to 14 occupied O -glycosylation sites. C , Stepping-energy CID spectrum of the same glycopeptide as in panel B using lower-energy stepping-energy CID (stepping energy is set to 60 and 80% each half of the time (instead of 80–140%) with a focus on the glycan-derived Y-ions.

    Article Snippet: The analytes were separated on a C18 analytical column (Acclaim PepMap RSLC, 75 μm × 15 cm, 2 μm, 100 Å, Dionex/Thermo Scientific) at 32 °C column oven temperature.

    Techniques: Pyrolysis Gas Chromatography, Mass Spectrometry, Generated, Derivative Assay