pentr 1a vector (Thermo Fisher)

Name:
Gateway pENTR 1A Dual Selection Vector
Description:
Gateway entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway entry clone The entry clone is ready for recombination with a destination vector to create an expression clone New pENTR Dual Selection vectors The Gateway entry vectors Table 1 offer the following • attL1 and attL2 sites for site specific recombination of the entry clone with a Gateway destination vector to ensure cloning of the gene of interest in the correct orientation for expression• Kozak consensus sequence for efficient translation initiation in eukaryotic systems• Ribosome binding site for efficient translation initiation in prokaryotic systems pENTR 1A Dual Selection pENTR 3C Dual Selection and pENTR 11 Dual Selection vectors only • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E coli • pUC origin for high copy replication and maintenance of the plasmid in E coli• Kanamycin resistance gene for selection in E coli• The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection ando Chloramphenicol selection in E coli• Kanamycin resistance gene for selection in E coli
Catalog Number:
a10462
Price:
None
Applications:
Cloning|Entry Vectors & Kits|Gateway Cloning
Category:
DNA Vectors Clones Purified Nucleic Acids Libraries
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Structured Review
Gateway entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway entry clone The entry clone is ready for recombination with a destination vector to create an expression clone New pENTR Dual Selection vectors The Gateway entry vectors Table 1 offer the following • attL1 and attL2 sites for site specific recombination of the entry clone with a Gateway destination vector to ensure cloning of the gene of interest in the correct orientation for expression• Kozak consensus sequence for efficient translation initiation in eukaryotic systems• Ribosome binding site for efficient translation initiation in prokaryotic systems pENTR 1A Dual Selection pENTR 3C Dual Selection and pENTR 11 Dual Selection vectors only • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E coli • pUC origin for high copy replication and maintenance of the plasmid in E coli• Kanamycin resistance gene for selection in E coli• The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection ando Chloramphenicol selection in E coli• Kanamycin resistance gene for selection in E coli
https://www.bioz.com/result/pentr 1a vector/product/Thermo Fisher
Average 99 stars, based on 10 article reviews
Price from $9.99 to $1999.99
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