Structured Review

Cayman Chemical pentostatin
( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or <t>pentostatin</t> (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.
Pentostatin, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentostatin/product/Cayman Chemical
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pentostatin - by Bioz Stars, 2024-04
91/100 stars

Images

1) Product Images from "Cellular sensing of extracellular purine nucleosides triggers an innate IFN-β response"

Article Title: Cellular sensing of extracellular purine nucleosides triggers an innate IFN-β response

Journal: Science Advances

doi: 10.1126/sciadv.aba3688

( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.
Figure Legend Snippet: ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.

Techniques Used: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Labeling, Transfection, Protein Concentration, Expressing


Structured Review

Cayman Chemical pentostatin
Pentostatin, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentostatin/product/Cayman Chemical
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pentostatin - by Bioz Stars, 2024-04
91/100 stars

Images

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Cayman Chemical pentostatin
    ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or <t>pentostatin</t> (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.
    Pentostatin, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentostatin/product/Cayman Chemical
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pentostatin - by Bioz Stars, 2024-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.

    Journal: Science Advances

    Article Title: Cellular sensing of extracellular purine nucleosides triggers an innate IFN-β response

    doi: 10.1126/sciadv.aba3688

    Figure Lengend Snippet: ( A ) ADA2 mRNA levels, measured by qRT-PCR, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( B ) Secreted ADA2 protein, measured by ELISA, in primary endothelial cells and U937 monocytic cells ( n = 3 technical replicates). ( C ) Secreted ADA2 protein, assessed by Western blotting of serum-free cell-conditioned supernatants, from HUVEC and U937 monocytic cells. ( D ) ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in whole-cell lysates (intracellular) or cell-conditioned supernatants (extracellular) from siControl, siADA1-, or siADA2-transfected HUVEC. Values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( E ) Expression levels of IRF3-driven or IFN-β–driven genes, measured by qRT-PCR, and extracellular ADA activity, measured by de novo conversion of 1 mM isotopically labeled dAdo to dIno in cell-conditioned supernatants, from siControl or siADA2-transfected HUVEC supplemented with rADA1 or rADA2 and pretreated with vehicle or pentostatin (10 μM for 30 min). Activity values are normalized to cell number and protein concentration ( n = 5 technical replicates). ( F ) IFN -β mRNA, measured by qRT-PCR, in U937 monocytic cells supplemented with adenosine (Ado) or deoxyadenosine (dAdo) (100 μM for 48 hours) ( n = 3 technical replicates). ( G ) IFN -β mRNA levels, measured by qRT-PCR, in U937 cocultured in Transwell inserts with siControl or siADA2-treated HUVEC for 48 hours ( n = 3 technical replicates). All results were replicated in three independent experiments. Values are presented as means ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001. HDVEC, dermal microvascular endothelial cells; HBVEC, brain microvascular endothelial cells; HKVEC, kidney microvascular endothelial cells.

    Article Snippet: Pentostatin was purchased from Cayman Chemicals, cycloleucine was from MP Biomedicals, and 2′-deoxyinosine, inosine, and hypoxanthine were from Alfa Aeser.

    Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Activity Assay, Labeling, Transfection, Protein Concentration, Expressing