penicillin  (Thermo Fisher)


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    Name:
    Penicillin G M I C Evaluator Strips M I C E
    Description:
    Effortlessly establish accurate Minimum Inhibitory Concentration MIC values manually using Thermo Scientific Oxoid Penicillin G M I C Evaluator Strips M I C E
    Catalog Number:
    ma0100d
    Price:
    None
    Category:
    Kits and Assays
    Applications:
    Clinical|Clinical Microbiology
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    Structured Review

    Thermo Fisher penicillin
    Effortlessly establish accurate Minimum Inhibitory Concentration MIC values manually using Thermo Scientific Oxoid Penicillin G M I C Evaluator Strips M I C E
    https://www.bioz.com/result/penicillin/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Modification:

    Article Title: Lipopolysaccharide Lowers Cholesteryl Ester Transfer Protein by Activating F4/80+Clec4f+Vsig4+Ly6C− Kupffer Cell Subsets
    Article Snippet: Monocytes were then purified using human CD14 magnetic beads and MACS® cell separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Monocytes were plated in 24‐well tissue culture plates at a density of 1×106 cells/mL (500 μL per well) and differentiated to macrophages for 6 days in Iscove's Modified Dulbecco's Medium (Sigma‐Aldrich) supplemented with 2 mmol/L l ‐glutamine, penicillin (100 U/mL), streptomycin (100 μg/mL) and 10% fetal calf serum (All Gibco, Waltham, MA) in the presence of 50 ng/mL macrophage colony stimulating factor (MCSF) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. On day 3, the medium was removed and substituted by fresh Iscove's Modified Dulbecco's Medium with 10% fetal calf serum and 50 ng/mL MCSF.

    Article Title: Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome
    Article Snippet: .. SHED were isolated from dental pulp tissues as described previously , and grown in a SHED culture medium consisting of Minimum Essential Medium Eagle Alpha Modification (Sigma-Aldrich, MO, USA) with 15% fetal bovine serum (Sigma-Aldrich), 100 µM L-ascorbic 2-phosphate (Wako Pure Chemical Industries, Osaka, Japan), 2 mM L -glutamine (Life Technologies, NY, USA), 100 U/mL penicillin (Life Technologies), 100 µg/mL streptomycin (Life Technologies) and 25 µg/mL Fungizone (Life Technologies) at 37 °C in 5% CO2 . ..

    Cell Culture:

    Article Title: Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells
    Article Snippet: Peripheral Blood Mononuclear Cells (PBMCs) were isolated by density gradient centrifugation using Lymphocyte separation medium (EurobioTM, Montpellier, France). .. Cells were washed three times with PBS, subsequently stimulated with 1 mg/ml phytohemagglutinin-P (PHA) (SigmaTM, Saint Louis, Missouri, USA) and cultured in DMEM supplemented with 1% (v/v) sodium pyruvate, non-essential aminoacids, vitamins, L-arginin, L-asparragin, folic acid, 10 mM Hepes, 50 mM 2-mercaptoethanol, 100 mg/ml streptomycin, 100 U/ml penicillin (Life Technologies, Carlsbad, CA, USA) and 10% heat-inactivated FBS (Gibco) at 37°C, in a 10% CO2 atmosphere. ..

    Isolation:

    Article Title: Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome
    Article Snippet: .. SHED were isolated from dental pulp tissues as described previously , and grown in a SHED culture medium consisting of Minimum Essential Medium Eagle Alpha Modification (Sigma-Aldrich, MO, USA) with 15% fetal bovine serum (Sigma-Aldrich), 100 µM L-ascorbic 2-phosphate (Wako Pure Chemical Industries, Osaka, Japan), 2 mM L -glutamine (Life Technologies, NY, USA), 100 U/mL penicillin (Life Technologies), 100 µg/mL streptomycin (Life Technologies) and 25 µg/mL Fungizone (Life Technologies) at 37 °C in 5% CO2 . ..

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  • 99
    Thermo Fisher dmem
    Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was <t>DMEM</t> + 10% <t>FBS.</t> a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99/100 stars
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    98
    Thermo Fisher lpds
    ATGL expression in macrophages and foam cells. Mouse peritoneal macrophages, human THP-1, and human monocyte-derived macrophages ( HMDM ) were cultivated in <t>DMEM,</t> 10% <t>LPDS</t> in the absence (control) or presence of 100 μg of acLDL/ml. Total RNA was isolated and reverse-transcribed, and mRNA expression of ATGL ( A and C ) and hormone-sensitive lipase ( HSL ) was determined by real time PCR, including murine hypoxanthine-guanine phosphoribosyltransferase or human ( hu ) porphobilinogen deaminase normalization ( A ). Untreated macrophages were arbitrarily set to 1. Data are expressed as mean values ( n = 3) ± S.E. of triplicate repeats. ***, p ≤ 0.001. B , cell extracts of macrophages, foam cells (40 μg per lane), and white adipose tissue ( WAT ) (10 and 40 μg per lane) were resolved by SDS-PAGE. Protein expression of ATGL and hormone-sensitive lipase were analyzed by Western blotting relative to the expression of β-actin. STD , standard.
    Lpds, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 1 article reviews
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    99
    Thermo Fisher 100 mm petri dish
    Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, <t>100</t> μm.
    100 Mm Petri Dish, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 mm petri dish/product/Thermo Fisher
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    Image Search Results


    Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was DMEM + 10% FBS. a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .

    Journal: Advanced Science

    Article Title: Jet‐Printing Microfluidic Devices on Demand, Jet‐Printing Microfluidic Devices on Demand

    doi: 10.1002/advs.202001854

    Figure Lengend Snippet: Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was DMEM + 10% FBS. a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .

    Article Snippet: Mouse embryonic stem (ES) cells (EK.CCE line, derived from a single XY blastocyst‐stage embryo of strain 129/Sv/Ec)[ ] were routinely cultured in DMEM supplemented with 15% FBS, 1% penicillin/streptomycin (Gibco, #15140122), 0.1 × 10−3 m 2‐mercaptoethanol, 1% glutamine (Invitrogen, Loughborough, UK), 1% minimum essential media nonessential amino acids (Gibco), 1 × 10−3 m sodium pyruvate, and 1000 U mL−1 leukemia inhibitory factor (LIF, ESGRO; Merck, Darmstadt, Germany; #L5158) on gelatin‐coated plates (Merck, #ES‐006‐B).

    Techniques: Generated

    ATGL expression in macrophages and foam cells. Mouse peritoneal macrophages, human THP-1, and human monocyte-derived macrophages ( HMDM ) were cultivated in DMEM, 10% LPDS in the absence (control) or presence of 100 μg of acLDL/ml. Total RNA was isolated and reverse-transcribed, and mRNA expression of ATGL ( A and C ) and hormone-sensitive lipase ( HSL ) was determined by real time PCR, including murine hypoxanthine-guanine phosphoribosyltransferase or human ( hu ) porphobilinogen deaminase normalization ( A ). Untreated macrophages were arbitrarily set to 1. Data are expressed as mean values ( n = 3) ± S.E. of triplicate repeats. ***, p ≤ 0.001. B , cell extracts of macrophages, foam cells (40 μg per lane), and white adipose tissue ( WAT ) (10 and 40 μg per lane) were resolved by SDS-PAGE. Protein expression of ATGL and hormone-sensitive lipase were analyzed by Western blotting relative to the expression of β-actin. STD , standard.

    Journal: The Journal of Biological Chemistry

    Article Title: Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase *

    doi: 10.1074/jbc.M110.107854

    Figure Lengend Snippet: ATGL expression in macrophages and foam cells. Mouse peritoneal macrophages, human THP-1, and human monocyte-derived macrophages ( HMDM ) were cultivated in DMEM, 10% LPDS in the absence (control) or presence of 100 μg of acLDL/ml. Total RNA was isolated and reverse-transcribed, and mRNA expression of ATGL ( A and C ) and hormone-sensitive lipase ( HSL ) was determined by real time PCR, including murine hypoxanthine-guanine phosphoribosyltransferase or human ( hu ) porphobilinogen deaminase normalization ( A ). Untreated macrophages were arbitrarily set to 1. Data are expressed as mean values ( n = 3) ± S.E. of triplicate repeats. ***, p ≤ 0.001. B , cell extracts of macrophages, foam cells (40 μg per lane), and white adipose tissue ( WAT ) (10 and 40 μg per lane) were resolved by SDS-PAGE. Protein expression of ATGL and hormone-sensitive lipase were analyzed by Western blotting relative to the expression of β-actin. STD , standard.

    Article Snippet: Macrophages were cultured in DMEM, 10% LPDS (Invitrogen) supplemented with 1% penicillin, 1% streptomycin for 2 h. Thereafter, cells were washed twice with PBS, and if not stated otherwise, the adherent macrophages were cultured in high glucose (25 mm ) DMEM containing 4 mm glutamine, 1 mm pyruvate, and 10% LPDS.

    Techniques: Expressing, Derivative Assay, Isolation, Real-time Polymerase Chain Reaction, SDS Page, Western Blot

    mRNA levels of mitochondrial genes in Atgl −/− and WT macrophages and foam cells. MPM were cultivated in DMEM, 10% LPDS in the absence or presence of 100 μg of acLDL/ml (foam cells). Total RNA was isolated from cells and reverse-transcribed, and mRNA levels of carnitine palmitoyltransferase ( CPT )1a ( A ) and long chain acyl-CoA dehydrogenase ( LCAD ) ( B ) were determined by real time PCR, including normalization to hypoxanthine-guanine phosphoribosyltransferase ( HPRT ) levels. Untreated MPM were arbitrarily set to 1. Data are expressed as mean values ± S.E. of triplicate repeats. C , long chain acyl-CoA. D , carnitine ester concentrations were determined in macrophages of Atgl −/− and WT mice. Data are expressed as mean values ( n = 4–5) ± S.E. correlated to protein concentrations. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase *

    doi: 10.1074/jbc.M110.107854

    Figure Lengend Snippet: mRNA levels of mitochondrial genes in Atgl −/− and WT macrophages and foam cells. MPM were cultivated in DMEM, 10% LPDS in the absence or presence of 100 μg of acLDL/ml (foam cells). Total RNA was isolated from cells and reverse-transcribed, and mRNA levels of carnitine palmitoyltransferase ( CPT )1a ( A ) and long chain acyl-CoA dehydrogenase ( LCAD ) ( B ) were determined by real time PCR, including normalization to hypoxanthine-guanine phosphoribosyltransferase ( HPRT ) levels. Untreated MPM were arbitrarily set to 1. Data are expressed as mean values ± S.E. of triplicate repeats. C , long chain acyl-CoA. D , carnitine ester concentrations were determined in macrophages of Atgl −/− and WT mice. Data are expressed as mean values ( n = 4–5) ± S.E. correlated to protein concentrations. *, p

    Article Snippet: Macrophages were cultured in DMEM, 10% LPDS (Invitrogen) supplemented with 1% penicillin, 1% streptomycin for 2 h. Thereafter, cells were washed twice with PBS, and if not stated otherwise, the adherent macrophages were cultured in high glucose (25 mm ) DMEM containing 4 mm glutamine, 1 mm pyruvate, and 10% LPDS.

    Techniques: Isolation, Cycling Probe Technology, Real-time Polymerase Chain Reaction, Mouse Assay

    Phagocytosis in Atgl −/− and WT macrophages. A , MPM from Atgl −/− and WT mice were cultivated in DMEM, 10% LPDS with 0, 6, and 25 m m glucose for 1 and 8 h, respectively. Phagocytosis of fluorescein-labeled E. coli particles is presented as mean values ( n = 6) ± S.E. of two independent experiments. Phagocytosis of WT cells was arbitrarily set to 100%. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase *

    doi: 10.1074/jbc.M110.107854

    Figure Lengend Snippet: Phagocytosis in Atgl −/− and WT macrophages. A , MPM from Atgl −/− and WT mice were cultivated in DMEM, 10% LPDS with 0, 6, and 25 m m glucose for 1 and 8 h, respectively. Phagocytosis of fluorescein-labeled E. coli particles is presented as mean values ( n = 6) ± S.E. of two independent experiments. Phagocytosis of WT cells was arbitrarily set to 100%. *, p

    Article Snippet: Macrophages were cultured in DMEM, 10% LPDS (Invitrogen) supplemented with 1% penicillin, 1% streptomycin for 2 h. Thereafter, cells were washed twice with PBS, and if not stated otherwise, the adherent macrophages were cultured in high glucose (25 mm ) DMEM containing 4 mm glutamine, 1 mm pyruvate, and 10% LPDS.

    Techniques: Mouse Assay, Labeling

    ATP concentrations and ADP/ATP ratios in Atgl −/− and WT macrophages. A , ATP levels in Atgl −/− and WT MPM were measured after cultivating the cells for 24 h in DMEM, 10% LPDS containing 0, 6, and 25 m m glucose. Oligomycin (0.5 μ m ) was used as an ATP-depletion control. Data are presented as mean values ( n = 4) ± S.E. ***, p ≤ 0.001. B , ADP/ATP ratios in Atgl −/− and WT MPM were determined using the EnzyLight TM ADP/ATP ratio assay kit. Data are expressed as mean values ( n = 4) ± S.E. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase *

    doi: 10.1074/jbc.M110.107854

    Figure Lengend Snippet: ATP concentrations and ADP/ATP ratios in Atgl −/− and WT macrophages. A , ATP levels in Atgl −/− and WT MPM were measured after cultivating the cells for 24 h in DMEM, 10% LPDS containing 0, 6, and 25 m m glucose. Oligomycin (0.5 μ m ) was used as an ATP-depletion control. Data are presented as mean values ( n = 4) ± S.E. ***, p ≤ 0.001. B , ADP/ATP ratios in Atgl −/− and WT MPM were determined using the EnzyLight TM ADP/ATP ratio assay kit. Data are expressed as mean values ( n = 4) ± S.E. *, p

    Article Snippet: Macrophages were cultured in DMEM, 10% LPDS (Invitrogen) supplemented with 1% penicillin, 1% streptomycin for 2 h. Thereafter, cells were washed twice with PBS, and if not stated otherwise, the adherent macrophages were cultured in high glucose (25 mm ) DMEM containing 4 mm glutamine, 1 mm pyruvate, and 10% LPDS.

    Techniques:

    Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, 100 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

    doi: 10.3390/ijms18112348

    Figure Lengend Snippet: Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, 100 μm.

    Article Snippet: Cell and Bioink Preparation Fibroblasts (CCD-1112SK, ATCC, Manassas, VA, USA) and HPV18-positive cervical cancer cells (HeLa; KCLB, Seoul, Korea) were cultured with a medium containing 10% FBS and 1% Pen Strep in a 100-mm Petri dish (Nunclon Delta Surface, Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay