penicillin streptomycin  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Shandon 10000 Autopsy Saw
    Description:
    Cut safely and reduce operator fatigue with Thermo Scientific Shandon 10000 Autopsy Saw
    Catalog Number:
    10000
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    Anatomical Pathology|Clinical
    Buy from Supplier


    Structured Review

    Thermo Fisher penicillin streptomycin
    Cut safely and reduce operator fatigue with Thermo Scientific Shandon 10000 Autopsy Saw
    https://www.bioz.com/result/penicillin streptomycin/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Bioprinting of stem cell expansion lattices
    Article Snippet: NPCs were cultured following a previously described protocol [ ]. .. Briefly, NPCs were cultured in Stemness Maintenance Medium (Neurobasal-A, 2% B27 Supplement with Vitamin A (Gibco), GlutaMAX (Gibco), 20 ng ml−1 FGF-2 (PeproTech), 20 ng ml−1 EGF (PeproTech), and 1% Pen/Strep (Gibco)) on tissue culture plastic coated with 10 μg mL−1 polyornithine (Sigma-Aldrich) and 5 mg mL−1 laminin (Gibco) at a seeding density of 10,000 NPCs cm−2 . ..

    Mutagenesis:

    Article Title: Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow
    Article Snippet: .. 35,000 or 10,000 Ly6D- CLPs (Lin− Sca+ cKit+ IL7R+ Thy1.2− Ly6D− ) were sorted from OcnCre;iDTR control and mutant donors (CD45.2-Pacific Blue), labeled with 10 µM CFDA-SE (Invitrogen) and 1 µg/ml VivoTag680 (VT680, VisEn Medical), respectively, mixed in a 1:1 ratio, and transplanted into each congenic SJL recipient (CD45.1-PE) by retro-orbital injection. .. The same number of progenitor cells sorted from CCR7−/− mice served as another control to compete with OcnCre;iDTR mutant donors.

    Labeling:

    Article Title: Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow
    Article Snippet: .. 35,000 or 10,000 Ly6D- CLPs (Lin− Sca+ cKit+ IL7R+ Thy1.2− Ly6D− ) were sorted from OcnCre;iDTR control and mutant donors (CD45.2-Pacific Blue), labeled with 10 µM CFDA-SE (Invitrogen) and 1 µg/ml VivoTag680 (VT680, VisEn Medical), respectively, mixed in a 1:1 ratio, and transplanted into each congenic SJL recipient (CD45.1-PE) by retro-orbital injection. .. The same number of progenitor cells sorted from CCR7−/− mice served as another control to compete with OcnCre;iDTR mutant donors.

    Injection:

    Article Title: Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow
    Article Snippet: .. 35,000 or 10,000 Ly6D- CLPs (Lin− Sca+ cKit+ IL7R+ Thy1.2− Ly6D− ) were sorted from OcnCre;iDTR control and mutant donors (CD45.2-Pacific Blue), labeled with 10 µM CFDA-SE (Invitrogen) and 1 µg/ml VivoTag680 (VT680, VisEn Medical), respectively, mixed in a 1:1 ratio, and transplanted into each congenic SJL recipient (CD45.1-PE) by retro-orbital injection. .. The same number of progenitor cells sorted from CCR7−/− mice served as another control to compete with OcnCre;iDTR mutant donors.

    Transfection:

    Article Title: Novel lincRNA SLINKY is a prognostic biomarker in kidney cancer
    Article Snippet: .. siRNA transfections and proliferation assays ccRCC cells were seeded (10,000 cells per well) in 6-well plates and transfected using Lipofectamine 2000 (Life Technologies). .. Two independent small interfering (si)RNAs targeting the first exon of SLINKY (On-TARGETplus, Dharmacon) were transfected at a final siRNA concentration of 50nM for 16 hrs (custom-designed siRNA sequences available in ).

    Multiple Displacement Amplification:

    Article Title: Molecular Imaging of the Translocator Protein (TSPO) in a Pre-Clinical Model of Breast Cancer
    Article Snippet: A Nikon Eclipse TE2000-U fluorescence microscope equipped with a mercury lamp, indocyanine green filter set and a Hamamatsu ORCA II BT 512 camera controlled by Metamorph v6.1 (Molecular Devices Corporation; Downingtown, PA) was used for imaging. .. MDA-MB-231 cells were plated at 10,000 cells per well in parafilm-wrapped 96 MicroWell™ Nunclon™Δ Optical Bottom Plates (Nalge Nunc International; Rochester, NY) and incubated under standard culture conditions for approximately 48 h. Immediately prior to experimentation, the cells were washed once with 37°C FBS-free medium to remove any dead cells and serum. .. The cells were then divided into four populations and evaluated in triplicate: (1) cells incubated with increasing concentrations of NIR-conPK11195 (1, 4, 7, 10, 40, 70, 100, 400, 700 nM, 1 μM) in FBS-free medium (100 μL volume), (2) cells simultaneously incubated with the same concentrations of NIR-conPK11195 and 100 µM PK 11195, (3) cells incubated with the same concentrations of free NIR dye, and (4) undosed cells as blanks.

    Incubation:

    Article Title: Molecular Imaging of the Translocator Protein (TSPO) in a Pre-Clinical Model of Breast Cancer
    Article Snippet: A Nikon Eclipse TE2000-U fluorescence microscope equipped with a mercury lamp, indocyanine green filter set and a Hamamatsu ORCA II BT 512 camera controlled by Metamorph v6.1 (Molecular Devices Corporation; Downingtown, PA) was used for imaging. .. MDA-MB-231 cells were plated at 10,000 cells per well in parafilm-wrapped 96 MicroWell™ Nunclon™Δ Optical Bottom Plates (Nalge Nunc International; Rochester, NY) and incubated under standard culture conditions for approximately 48 h. Immediately prior to experimentation, the cells were washed once with 37°C FBS-free medium to remove any dead cells and serum. .. The cells were then divided into four populations and evaluated in triplicate: (1) cells incubated with increasing concentrations of NIR-conPK11195 (1, 4, 7, 10, 40, 70, 100, 400, 700 nM, 1 μM) in FBS-free medium (100 μL volume), (2) cells simultaneously incubated with the same concentrations of NIR-conPK11195 and 100 µM PK 11195, (3) cells incubated with the same concentrations of free NIR dye, and (4) undosed cells as blanks.

    Real-time Polymerase Chain Reaction:

    Article Title: Seven Novel Probe Systems for Real-Time PCR Provide Absolute Single-Base Discrimination, Higher Signaling, and Generic Components
    Article Snippet: In Universal probe assays, the concentration of linker primer used in the first-step reaction was 20 nmol/L, and Universal probe and antiprobe concentrations were 200 and 400 nmol/L, respectively. .. We typically used 10 ng of viral cDNA or 1000 to 10,000 copies of ultramer template per reaction. qPCR reactions were performed in 25-μL volumes containing 12.5 μL of 2X HotStart-IT Probe qPCR Master Mix (Affymetrix, Inc., Santa Clara, CA), the indicated template, primer, probe, and/or antiprobe concentrations, and additional MgCl2 and dNTPs (added at final concentrations of 5 and 0.1 mmol/L, respectively). .. Typical thermal cycling conditions are 95°C, 5 minutes, and then 40 cycles of either two-step PCR (denaturation at 95°C for 15 seconds, and annealing/extension at 58°C for 30 to 60 seconds), or three-step PCR (denaturation at 95°C, 15 seconds; annealing at 58°C, 45 seconds; and extension at 72°C, 45 seconds).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dmem
    Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was <t>DMEM</t> + 10% <t>FBS.</t> a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher fura 2 am
    Compound K induced apoptosis via Ca 2+ mediated caspase-12 activation. (A) Cytosolic Ca 2+ levels were measured by <t>Fura-2/AM</t> fluorescence dye. Compound K treated 30 min after the beginning of the reading as indicated by the arrow. (B, C, D) Effect of the RyR channel antagonist (dantrolene), IP 3 R channel antagonist (2-APB), or Ca 2+ chelator (BAPTA-AM) on apoptosis was determined by testing the costaining with PI and FITC-conjugated annexin V, and the translocation of phosphatidylserine was detected by flow cytometry. Data are presented as means ± SD of three independent experiments. (E, F) Cells were pretreated with 5μM dantrolene or 2.5μM BAPTA-AM for 1 h and then treated with 15μM of compound K for 48 h. m-Calpain, caspase-12, and procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. (G) Cells were pretreated with 30μM 2-APB for 1 h and were then treated with 15μM of compound K for 48 h. Procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. *** p
    Fura 2 Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fura 2 am/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fura 2 am - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher dynabeads human t activator cd3 cd28
    Exogenous fatty acids dose-dependently alter human CD4 + T cell membrane order and giant plasma membrane vesicle (GPMV) phase separation. Human pan CD4 + T cells isolated from buffy-coat leucocytes were incubated with various doses (0–50 μM) of linoleic acid (LA), EPA and DHA conjugated with bovine serum albumin for 2 d. Cultures were further stimulated for 3 d with <t>Dynabeads</t> Human T-Activator <t>CD3/CD28</t> at the bead-to-cell ratio of 1:1, in the presence of their respective FA. Membrane order was measured following 5 d of total incubation. Data are expressed as the change in generalised polarisation (ΔGP) compared with the untreated (UT) group in (A) whole cells and (B) GPMV. (C) n -3 PUFA significantly increased numbers of GPMV with phase separation. (D) Representative images of GPMV showing no phase separation (UT and LA 50 μM) and phase separation (DHA 50 μM and EPA 50 μM). White dashed line represents region of interest identifying GPMV and used to determine membrane order. Note that GPMV rarely detach fully from the cell. Scale bar equals 2 μm. (E) Average histogram of normalised GP values obtained from non-phase separated ( ) and phase separated ( ) GPMV from LA 50 μM and EPA 50 μM treated cells, respectively. Values are as means ( n 50–183, pooled from three separate experiments), with their standard errors. a,b,c,d Mean values with unlike letters are significantly different ( P
    Dynabeads Human T Activator Cd3 Cd28, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads human t activator cd3 cd28/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dynabeads human t activator cd3 cd28 - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was DMEM + 10% FBS. a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .

    Journal: Advanced Science

    Article Title: Jet‐Printing Microfluidic Devices on Demand, Jet‐Printing Microfluidic Devices on Demand

    doi: 10.1002/advs.202001854

    Figure Lengend Snippet: Varying jetting parameters. Unless stated otherwise throughout the paper, Q ˙ = 8 µL s −1 , H (height of nozzle above substrate) = 0.4 mm, D i (diameter of jetting nozzle) = 60 µm, V traverse (lateral traverse speed) = 10 mm s −1 , and media was DMEM + 10% FBS. a) Overview. A submerged FC40 microjet drives media off the substrate to create an FC40 wall, and some parameters varied are indicated. b) Controlling wall width (red symbols: continuous walls fail to form) by varying: i) jet height (inset: images of walls when H = 0.2 and 0.8 mm). The jet width increases with distance from the nozzle, so lowering the nozzle yields progressively narrower wall. ii) Flow rate and nozzle diameter. Wall width increases as jet momentum increases (by increasing flowrate or reducing nozzle diameter). iii) Traverse velocity and serum content. Proteins in the serum causes an increased adhension force at the pinning lines, so the “sweeping” action of the jet momentum is diminished, resulting in smaller wall widths. c) Varying wall width by overlapping walls. Wall width can be increased by setting the separation between jetting paths to be smaller than the wall width generated by one pass. d) Making conduits with different widths (walls all have equal widths) by varying the distance between consecutive paths. H = 0.3 mm, Q ˙ = 5 µL s −1 , D nozzle = 60 µm, and V traverse = 1.8 mm s −1 .

    Article Snippet: Mouse embryonic stem (ES) cells (EK.CCE line, derived from a single XY blastocyst‐stage embryo of strain 129/Sv/Ec)[ ] were routinely cultured in DMEM supplemented with 15% FBS, 1% penicillin/streptomycin (Gibco, #15140122), 0.1 × 10−3 m 2‐mercaptoethanol, 1% glutamine (Invitrogen, Loughborough, UK), 1% minimum essential media nonessential amino acids (Gibco), 1 × 10−3 m sodium pyruvate, and 1000 U mL−1 leukemia inhibitory factor (LIF, ESGRO; Merck, Darmstadt, Germany; #L5158) on gelatin‐coated plates (Merck, #ES‐006‐B).

    Techniques: Generated

    Compound K induced apoptosis via Ca 2+ mediated caspase-12 activation. (A) Cytosolic Ca 2+ levels were measured by Fura-2/AM fluorescence dye. Compound K treated 30 min after the beginning of the reading as indicated by the arrow. (B, C, D) Effect of the RyR channel antagonist (dantrolene), IP 3 R channel antagonist (2-APB), or Ca 2+ chelator (BAPTA-AM) on apoptosis was determined by testing the costaining with PI and FITC-conjugated annexin V, and the translocation of phosphatidylserine was detected by flow cytometry. Data are presented as means ± SD of three independent experiments. (E, F) Cells were pretreated with 5μM dantrolene or 2.5μM BAPTA-AM for 1 h and then treated with 15μM of compound K for 48 h. m-Calpain, caspase-12, and procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. (G) Cells were pretreated with 30μM 2-APB for 1 h and were then treated with 15μM of compound K for 48 h. Procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. *** p

    Journal: Journal of Ginseng Research

    Article Title: Compound K induced apoptosis via endoplasmic reticulum Ca2+ release through ryanodine receptor in human lung cancer cells

    doi: 10.1016/j.jgr.2017.01.015

    Figure Lengend Snippet: Compound K induced apoptosis via Ca 2+ mediated caspase-12 activation. (A) Cytosolic Ca 2+ levels were measured by Fura-2/AM fluorescence dye. Compound K treated 30 min after the beginning of the reading as indicated by the arrow. (B, C, D) Effect of the RyR channel antagonist (dantrolene), IP 3 R channel antagonist (2-APB), or Ca 2+ chelator (BAPTA-AM) on apoptosis was determined by testing the costaining with PI and FITC-conjugated annexin V, and the translocation of phosphatidylserine was detected by flow cytometry. Data are presented as means ± SD of three independent experiments. (E, F) Cells were pretreated with 5μM dantrolene or 2.5μM BAPTA-AM for 1 h and then treated with 15μM of compound K for 48 h. m-Calpain, caspase-12, and procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. (G) Cells were pretreated with 30μM 2-APB for 1 h and were then treated with 15μM of compound K for 48 h. Procaspase-3 were analyzed by Western blotting and β-actin was used as an internal control. *** p

    Article Snippet: RPMI 1640 medium, DMEM medium, fetal bovine serum (FBS), penicillin, streptomycin, and Fura-2/AM were obtained from Life Technologies Inc (Chicago, IL, USA).

    Techniques: Activation Assay, Fluorescence, Translocation Assay, Flow Cytometry, Cytometry, Western Blot

    Exogenous fatty acids dose-dependently alter human CD4 + T cell membrane order and giant plasma membrane vesicle (GPMV) phase separation. Human pan CD4 + T cells isolated from buffy-coat leucocytes were incubated with various doses (0–50 μM) of linoleic acid (LA), EPA and DHA conjugated with bovine serum albumin for 2 d. Cultures were further stimulated for 3 d with Dynabeads Human T-Activator CD3/CD28 at the bead-to-cell ratio of 1:1, in the presence of their respective FA. Membrane order was measured following 5 d of total incubation. Data are expressed as the change in generalised polarisation (ΔGP) compared with the untreated (UT) group in (A) whole cells and (B) GPMV. (C) n -3 PUFA significantly increased numbers of GPMV with phase separation. (D) Representative images of GPMV showing no phase separation (UT and LA 50 μM) and phase separation (DHA 50 μM and EPA 50 μM). White dashed line represents region of interest identifying GPMV and used to determine membrane order. Note that GPMV rarely detach fully from the cell. Scale bar equals 2 μm. (E) Average histogram of normalised GP values obtained from non-phase separated ( ) and phase separated ( ) GPMV from LA 50 μM and EPA 50 μM treated cells, respectively. Values are as means ( n 50–183, pooled from three separate experiments), with their standard errors. a,b,c,d Mean values with unlike letters are significantly different ( P

    Journal: The British journal of nutrition

    Article Title: Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA

    doi: 10.1017/S0007114517003385

    Figure Lengend Snippet: Exogenous fatty acids dose-dependently alter human CD4 + T cell membrane order and giant plasma membrane vesicle (GPMV) phase separation. Human pan CD4 + T cells isolated from buffy-coat leucocytes were incubated with various doses (0–50 μM) of linoleic acid (LA), EPA and DHA conjugated with bovine serum albumin for 2 d. Cultures were further stimulated for 3 d with Dynabeads Human T-Activator CD3/CD28 at the bead-to-cell ratio of 1:1, in the presence of their respective FA. Membrane order was measured following 5 d of total incubation. Data are expressed as the change in generalised polarisation (ΔGP) compared with the untreated (UT) group in (A) whole cells and (B) GPMV. (C) n -3 PUFA significantly increased numbers of GPMV with phase separation. (D) Representative images of GPMV showing no phase separation (UT and LA 50 μM) and phase separation (DHA 50 μM and EPA 50 μM). White dashed line represents region of interest identifying GPMV and used to determine membrane order. Note that GPMV rarely detach fully from the cell. Scale bar equals 2 μm. (E) Average histogram of normalised GP values obtained from non-phase separated ( ) and phase separated ( ) GPMV from LA 50 μM and EPA 50 μM treated cells, respectively. Values are as means ( n 50–183, pooled from three separate experiments), with their standard errors. a,b,c,d Mean values with unlike letters are significantly different ( P

    Article Snippet: RPMI 1640 medium, Leibovitz medium, FBS, Glutamax, penicillin, streptomycin and Dynabeads Human T-Activator CD3/CD28 were purchased from Gibco.

    Techniques: Isolation, Incubation