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Millipore penicillin streptomycin
Penicillin Streptomycin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/penicillin streptomycin/product/Millipore
Average 99 stars, based on 1 article reviews
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penicillin streptomycin - by Bioz Stars, 2021-03
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Modification:

Article Title: Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes
Article Snippet: .. Dulbecco's modified eagle's medium (DMEM), phosphate-buffered saline (PBS), penicillin-streptomycin, biliverdin and polybrene were also obtained from Sigma Aldrich. .. Foetal bovine serum (FBS) was purchased from Life-Technologies.

Article Title: Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine
Article Snippet: .. Materials and reagents Cell culture medium (Dulbecco’s modified eagle medium, 4500 mg/l glucose, 0.584 g/l l -glutamine, DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin–EDTA, and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Collagenase Type II and trypan blue were purchased from Biochrom/Merck (Darmstadt, Germany).

Article Title: The relationship between terminal functionalization and molecular weight of a gene delivery polymer and transfection efficacy in mammary epithelial 2-D cultures and 3-D organotypic cultures
Article Snippet: The NTA analysis gives a direct number-averaged distribution of the particle size as well as absolute particle concentration. .. EPH4 cells [ ] were cultured at 37 °C and 5% CO2 in EPH4 media (Dulbecco’s modified Eagle’s medium/F12 with 10% FBS and 1% v/v penicillin/streptomycin (Sigma P0781)). .. After polymer transfection, cells were washed twice with 1 mL DMEM and finally incubated in fresh EPH4 media for 48 hours post transfection.

Transfection:

Article Title: Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification
Article Snippet: Crude lysates were clarified by centrifugation (11000 g for 10 min at 4°C) and separate with SDS-PAGE gel after denaturation in the presence of loading buffer. .. Eukaryotic cell transfection and cell lyses Murine myocytes (C2C12) and HeLa cells were grown in low glucose medium (1 g/L glucose D-6046, Sigma Aldrich) supplied with FBS 10% (F-0804, Sigma Aldrich) and penicillin and streptomycin (P-4333, Sigma Aldrich) and when occurred, C2C12 cells were grown in high glucose medium (4.5 g/L glucose D-5671). .. For transfection, 40%–50% confluent cells were transfected with the according plasmids using lipofectamine (R-0531, TurboFect transfection Reagents, Thermo Fischer Scientific).

Staining:

Article Title: CXCR6 regulates the recruitment of pro-inflammatory IL-17A-producing T cells into atherosclerotic aortas
Article Snippet: .. To re-stimulate the cell suspensions for intracellular cytokine staining, the cells were cultured for 5h at 37°C with complete RPMI1640 (10% FBS, 2% penicillin/streptomycin) supplemented with 10ng ml–1 PMA, 500ng ml–1 Ionomycin C and 600ng ml–1 Brefeldin A (Sigma-Aldrich). .. To stain the re-stimulated cells, the single-cell suspensions were pre-incubated with anti-mouse CD16/32 antibodies (10min, room temperature), and stained with the following antibodies: CD45-Pacific Orange (Life Technologies), CXCR3-PerCP Cy5.5, CCR6-APC, CD3ε-APC Cy7, TCRβ-APC, TCRγδ-eF450 (all from eBioscience) or appropriate isotype controls.

Cell Culture:

Article Title: CXCR6 regulates the recruitment of pro-inflammatory IL-17A-producing T cells into atherosclerotic aortas
Article Snippet: .. To re-stimulate the cell suspensions for intracellular cytokine staining, the cells were cultured for 5h at 37°C with complete RPMI1640 (10% FBS, 2% penicillin/streptomycin) supplemented with 10ng ml–1 PMA, 500ng ml–1 Ionomycin C and 600ng ml–1 Brefeldin A (Sigma-Aldrich). .. To stain the re-stimulated cells, the single-cell suspensions were pre-incubated with anti-mouse CD16/32 antibodies (10min, room temperature), and stained with the following antibodies: CD45-Pacific Orange (Life Technologies), CXCR3-PerCP Cy5.5, CCR6-APC, CD3ε-APC Cy7, TCRβ-APC, TCRγδ-eF450 (all from eBioscience) or appropriate isotype controls.

Article Title: Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine
Article Snippet: .. Materials and reagents Cell culture medium (Dulbecco’s modified eagle medium, 4500 mg/l glucose, 0.584 g/l l -glutamine, DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin–EDTA, and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). .. Collagenase Type II and trypan blue were purchased from Biochrom/Merck (Darmstadt, Germany).

Article Title: The relationship between terminal functionalization and molecular weight of a gene delivery polymer and transfection efficacy in mammary epithelial 2-D cultures and 3-D organotypic cultures
Article Snippet: The NTA analysis gives a direct number-averaged distribution of the particle size as well as absolute particle concentration. .. EPH4 cells [ ] were cultured at 37 °C and 5% CO2 in EPH4 media (Dulbecco’s modified Eagle’s medium/F12 with 10% FBS and 1% v/v penicillin/streptomycin (Sigma P0781)). .. After polymer transfection, cells were washed twice with 1 mL DMEM and finally incubated in fresh EPH4 media for 48 hours post transfection.

Synthesized:

Article Title: A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR)
Article Snippet: .. To label newly synthesized peptides in infected cells growing on glass coverslips, cells were incubated for 5 min at 37°C in puromycylation medium (DMEM-F12, 10% FBS, 1% penicillin-streptomycin supplemented with 91 μM puromycin [Sigma-Aldrich] and 208 μM emetine [Sigma-Aldrich]) as previously described ( ). .. As a negative control, some cells were pretreated with complete medium containing 500 μM sodium arsenite (Sigma-Aldrich) for 15 min at 37°C before labeling with puromycylation medium.

Infection:

Article Title: A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR)
Article Snippet: .. To label newly synthesized peptides in infected cells growing on glass coverslips, cells were incubated for 5 min at 37°C in puromycylation medium (DMEM-F12, 10% FBS, 1% penicillin-streptomycin supplemented with 91 μM puromycin [Sigma-Aldrich] and 208 μM emetine [Sigma-Aldrich]) as previously described ( ). .. As a negative control, some cells were pretreated with complete medium containing 500 μM sodium arsenite (Sigma-Aldrich) for 15 min at 37°C before labeling with puromycylation medium.

Incubation:

Article Title: A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR)
Article Snippet: .. To label newly synthesized peptides in infected cells growing on glass coverslips, cells were incubated for 5 min at 37°C in puromycylation medium (DMEM-F12, 10% FBS, 1% penicillin-streptomycin supplemented with 91 μM puromycin [Sigma-Aldrich] and 208 μM emetine [Sigma-Aldrich]) as previously described ( ). .. As a negative control, some cells were pretreated with complete medium containing 500 μM sodium arsenite (Sigma-Aldrich) for 15 min at 37°C before labeling with puromycylation medium.

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  • 95
    Millipore human fgf2
    Oligodendrocyte differentiation protocol. (A) Schematic representation of spinal cord meningeal biopsy isolation for organotypic culture. Spinal cord was dissected from adult SD rat and 1 cm of meningeal tissue was isolated and plated in neurosphere expansion medium (NS, day 0). (B) Time course representation of the oligodendrocyte differentiation protocol from spinal cord meningeal biopsy. From day 0 to day 10: neurosphere expansion (NS), from day 10 to day 20: oligosphere culture (Step Go1); from day 20 to day 30: oligodendrocyte differentiation (Step Go2); from day 30 to day 33: oligodendrocyte maturation (Step Go3). Images show meningeal-derived differentiating oligodendrocyte morphology at each stage of the protocol. Insets in (B) are higher magnification images of representative cells in the boxes. Pictures in (B) are brightfield images. (C) Number of meningeal-derived cells in culture, calculated for every experimental replicate ( n = 4), present at each stage of the differentiation protocol. Data are presented as mean ± SEM. NSCs: neural stem cells; <t>FGF2:</t> human basic fibroblast growth factor; EGF: epidermal growth factor; PDGF-AA: platelet-derived growth factor type AA; T3: 3,3′,5-triiodo- L -thyronine. Scale bars: 50 μm.
    Human Fgf2, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fgf2/product/Millipore
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    human fgf2 - by Bioz Stars, 2021-03
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    97
    Millipore 5 o caffeoylquinic acid
    Effect of <t>5-O-caffeoylquinic</t> acid and epigallocatechin-3-gallate (EGCG) on lipid peroxidation. (A) Inhibitory effect on H 2 O 2 -induced membrane damage in PC12 cells. (B) Inhibitory effect on lipid peroxidation using mouse whole brain homogenates. Results shown are means±SD (n=3). Different small letters indicate significant differences.
    5 O Caffeoylquinic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 o caffeoylquinic acid/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Millipore hygromycin b
    Detection of Mcm2 at DS of  oriP  by ChIP. ( A ) Diagram of p818, a 12.6-kb plasmid carrying  oriP  and flanking sequences (light gray), the EBNA1 gene and flanking sequences (black), and hygromycin B-resistance cassette (hph, dark gray). The regions that were amplified by PCR for ChIP are indicated inside the circle. The distal end of the control region, E1 3′, is 560 bp from the edge of FR and 2.1 kb from DS, whereas E1 5′ is 2.1 kb farther away from  oriP . hph is about 6 kb from  oriP.  ( B ) ChIP of chromatin from 293 cells carrying p818. Chromatin (30 μg) was precipitated by using antibodies to Orc2, Mcm2, or EBNA1 or by using nonimmune rabbit IgG, as indicated. Either 1/10th (lanes 2, 4, and 6) or 1/50th (lanes 1, 3, and 5) of the recovered DNA was amplified by PCR to detect the four indicated regions of the plasmid. DNA from the chromatin preparation was tested in lanes 6–8, by using 6.4, 32, and 160 ng, respectively. PCR products were separated by agarose-gel electrophoresis and detected by staining with SYBR Green. A reverse image is shown. M, marker. The specific PCR products ranged from 184 bp (hph) to 435 bp (DS).
    Hygromycin B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hygromycin b/product/Millipore
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    94
    Millipore 4ht
    EBNA3C upregulation of TCL1 requires RBP-Jκ interaction. (A) Western blot for EBNA3C-HT (E3CHT), TCL1, and tubulin of EBNA3C-HT LCLs at days 0 and 7 after growth in the presence of <t>4HT</t> and at days 0, 7, and 14 after growth in the absence of 4HT.
    4ht, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Oligodendrocyte differentiation protocol. (A) Schematic representation of spinal cord meningeal biopsy isolation for organotypic culture. Spinal cord was dissected from adult SD rat and 1 cm of meningeal tissue was isolated and plated in neurosphere expansion medium (NS, day 0). (B) Time course representation of the oligodendrocyte differentiation protocol from spinal cord meningeal biopsy. From day 0 to day 10: neurosphere expansion (NS), from day 10 to day 20: oligosphere culture (Step Go1); from day 20 to day 30: oligodendrocyte differentiation (Step Go2); from day 30 to day 33: oligodendrocyte maturation (Step Go3). Images show meningeal-derived differentiating oligodendrocyte morphology at each stage of the protocol. Insets in (B) are higher magnification images of representative cells in the boxes. Pictures in (B) are brightfield images. (C) Number of meningeal-derived cells in culture, calculated for every experimental replicate ( n = 4), present at each stage of the differentiation protocol. Data are presented as mean ± SEM. NSCs: neural stem cells; FGF2: human basic fibroblast growth factor; EGF: epidermal growth factor; PDGF-AA: platelet-derived growth factor type AA; T3: 3,3′,5-triiodo- L -thyronine. Scale bars: 50 μm.

    Journal: Frontiers in Pharmacology

    Article Title: High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy

    doi: 10.3389/fphar.2017.00703

    Figure Lengend Snippet: Oligodendrocyte differentiation protocol. (A) Schematic representation of spinal cord meningeal biopsy isolation for organotypic culture. Spinal cord was dissected from adult SD rat and 1 cm of meningeal tissue was isolated and plated in neurosphere expansion medium (NS, day 0). (B) Time course representation of the oligodendrocyte differentiation protocol from spinal cord meningeal biopsy. From day 0 to day 10: neurosphere expansion (NS), from day 10 to day 20: oligosphere culture (Step Go1); from day 20 to day 30: oligodendrocyte differentiation (Step Go2); from day 30 to day 33: oligodendrocyte maturation (Step Go3). Images show meningeal-derived differentiating oligodendrocyte morphology at each stage of the protocol. Insets in (B) are higher magnification images of representative cells in the boxes. Pictures in (B) are brightfield images. (C) Number of meningeal-derived cells in culture, calculated for every experimental replicate ( n = 4), present at each stage of the differentiation protocol. Data are presented as mean ± SEM. NSCs: neural stem cells; FGF2: human basic fibroblast growth factor; EGF: epidermal growth factor; PDGF-AA: platelet-derived growth factor type AA; T3: 3,3′,5-triiodo- L -thyronine. Scale bars: 50 μm.

    Article Snippet: Step Go2 Medium Neurobasal medium, 2% B27 supplement, 2 mM glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin, 20 ng/ml human FGF2, 5 ng/ml human PDGF-AA and 15 nM 3,3′,5-triiodo-L -thyronine (T3) (Sigma–Aldrich).

    Techniques: Isolation, Derivative Assay

    Effect of 5-O-caffeoylquinic acid and epigallocatechin-3-gallate (EGCG) on lipid peroxidation. (A) Inhibitory effect on H 2 O 2 -induced membrane damage in PC12 cells. (B) Inhibitory effect on lipid peroxidation using mouse whole brain homogenates. Results shown are means±SD (n=3). Different small letters indicate significant differences.

    Journal: Preventive Nutrition and Food Science

    Article Title: Antioxidant and Neuronal Cell Protective Effects of Columbia Arabica Coffee with Different Roasting Conditions

    doi: 10.3746/pnf.2013.18.1.030

    Figure Lengend Snippet: Effect of 5-O-caffeoylquinic acid and epigallocatechin-3-gallate (EGCG) on lipid peroxidation. (A) Inhibitory effect on H 2 O 2 -induced membrane damage in PC12 cells. (B) Inhibitory effect on lipid peroxidation using mouse whole brain homogenates. Results shown are means±SD (n=3). Different small letters indicate significant differences.

    Article Snippet: Materials Folin-Ciocalteu’s phenol reagent, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,4,6-tripyridyl-S-triazine (TPTZ), trichloroacetic acid (TCA), thiobarbituric acid (TBA), vitamin C, α-tocopherol, catechin, 2-[4-(2-hydroxyethyl) piperazin-1-yl]ethanesulfonic acid (HEPES), sodium bicarbonate, penicillin, streptomycin, 5-O-caffeoylquinic acid, syringic acid, epigallocatechin gallate, ferrous sulfate (FeSO4 ), hydrogen peroxide (H2 O2 ), dimethyl sulfoxide (DMSO), 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay kit, lactate dehydrogenase (LDH) assay kit and all solvents used were of analytical grade and purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques:

    HPLC chromatograms of various extracts obtained from medium roasted coffee beans. (A) Phenolics as compound standards, (B) Extracts obtained from medium roasted coffee beans. Retention times: gallic acid, 4.56 min; protocatechuic acid, 7.34 min; 5-O-caffeoylquinic acid, 9.37 min; vanillic acid, 11.65 min; caffeic acid, 11.92 min; syringic acid, 12.35 min.

    Journal: Preventive Nutrition and Food Science

    Article Title: Antioxidant and Neuronal Cell Protective Effects of Columbia Arabica Coffee with Different Roasting Conditions

    doi: 10.3746/pnf.2013.18.1.030

    Figure Lengend Snippet: HPLC chromatograms of various extracts obtained from medium roasted coffee beans. (A) Phenolics as compound standards, (B) Extracts obtained from medium roasted coffee beans. Retention times: gallic acid, 4.56 min; protocatechuic acid, 7.34 min; 5-O-caffeoylquinic acid, 9.37 min; vanillic acid, 11.65 min; caffeic acid, 11.92 min; syringic acid, 12.35 min.

    Article Snippet: Materials Folin-Ciocalteu’s phenol reagent, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,4,6-tripyridyl-S-triazine (TPTZ), trichloroacetic acid (TCA), thiobarbituric acid (TBA), vitamin C, α-tocopherol, catechin, 2-[4-(2-hydroxyethyl) piperazin-1-yl]ethanesulfonic acid (HEPES), sodium bicarbonate, penicillin, streptomycin, 5-O-caffeoylquinic acid, syringic acid, epigallocatechin gallate, ferrous sulfate (FeSO4 ), hydrogen peroxide (H2 O2 ), dimethyl sulfoxide (DMSO), 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay kit, lactate dehydrogenase (LDH) assay kit and all solvents used were of analytical grade and purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: High Performance Liquid Chromatography

    Detection of Mcm2 at DS of  oriP  by ChIP. ( A ) Diagram of p818, a 12.6-kb plasmid carrying  oriP  and flanking sequences (light gray), the EBNA1 gene and flanking sequences (black), and hygromycin B-resistance cassette (hph, dark gray). The regions that were amplified by PCR for ChIP are indicated inside the circle. The distal end of the control region, E1 3′, is 560 bp from the edge of FR and 2.1 kb from DS, whereas E1 5′ is 2.1 kb farther away from  oriP . hph is about 6 kb from  oriP.  ( B ) ChIP of chromatin from 293 cells carrying p818. Chromatin (30 μg) was precipitated by using antibodies to Orc2, Mcm2, or EBNA1 or by using nonimmune rabbit IgG, as indicated. Either 1/10th (lanes 2, 4, and 6) or 1/50th (lanes 1, 3, and 5) of the recovered DNA was amplified by PCR to detect the four indicated regions of the plasmid. DNA from the chromatin preparation was tested in lanes 6–8, by using 6.4, 32, and 160 ng, respectively. PCR products were separated by agarose-gel electrophoresis and detected by staining with SYBR Green. A reverse image is shown. M, marker. The specific PCR products ranged from 184 bp (hph) to 435 bp (DS).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus

    doi: 10.1073/pnas.181347998

    Figure Lengend Snippet: Detection of Mcm2 at DS of oriP by ChIP. ( A ) Diagram of p818, a 12.6-kb plasmid carrying oriP and flanking sequences (light gray), the EBNA1 gene and flanking sequences (black), and hygromycin B-resistance cassette (hph, dark gray). The regions that were amplified by PCR for ChIP are indicated inside the circle. The distal end of the control region, E1 3′, is 560 bp from the edge of FR and 2.1 kb from DS, whereas E1 5′ is 2.1 kb farther away from oriP . hph is about 6 kb from oriP. ( B ) ChIP of chromatin from 293 cells carrying p818. Chromatin (30 μg) was precipitated by using antibodies to Orc2, Mcm2, or EBNA1 or by using nonimmune rabbit IgG, as indicated. Either 1/10th (lanes 2, 4, and 6) or 1/50th (lanes 1, 3, and 5) of the recovered DNA was amplified by PCR to detect the four indicated regions of the plasmid. DNA from the chromatin preparation was tested in lanes 6–8, by using 6.4, 32, and 160 ng, respectively. PCR products were separated by agarose-gel electrophoresis and detected by staining with SYBR Green. A reverse image is shown. M, marker. The specific PCR products ranged from 184 bp (hph) to 435 bp (DS).

    Article Snippet: A clone of the EBV-negative Burkitt's lymphoma cell line, DG75, that is close to tetraploid and carries p818 at about 50 copies per cell was obtained and maintained in RPMI medium supplemented with 9% FBS, penicillin and streptomycin, and hygromycin B at 300 μg/ml. p818 (Fig. A ) is a 12.6-kb plasmid similar to p201 ( ) but contains 4 kb of additional EBV DNA extending rightward from oriP to the Bam HI site at 13215.

    Techniques: Chromatin Immunoprecipitation, Plasmid Preparation, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, SYBR Green Assay, Marker

    EBNA3C upregulation of TCL1 requires RBP-Jκ interaction. (A) Western blot for EBNA3C-HT (E3CHT), TCL1, and tubulin of EBNA3C-HT LCLs at days 0 and 7 after growth in the presence of 4HT and at days 0, 7, and 14 after growth in the absence of 4HT.

    Journal:

    Article Title: Epstein-Barr Virus Nuclear Protein 3C Domains Necessary for Lymphoblastoid Cell Growth: Interaction with RBP-J? Regulates TCL1 ▿

    doi: 10.1128/JVI.01403-09

    Figure Lengend Snippet: EBNA3C upregulation of TCL1 requires RBP-Jκ interaction. (A) Western blot for EBNA3C-HT (E3CHT), TCL1, and tubulin of EBNA3C-HT LCLs at days 0 and 7 after growth in the presence of 4HT and at days 0, 7, and 14 after growth in the absence of 4HT.

    Article Snippet: LCLs were maintained in RPMI 1640 medium (Gibco, Grand Island, NY) supplemented with 15% fetal bovine serum (Gemini Bio, West Sacramento, CA), l -glutamine, streptomycin, penicillin, and 400 nM 4HT (Sigma, St. Louis, MO).

    Techniques: Western Blot