penicillin streptomycin  (GE Healthcare)

 
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    Name:
    Penicillin Streptomycin solution
    Description:

    Catalog Number:
    sv30010
    Price:
    18.36 USD
    Size:
    100 mL
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    GE Healthcare penicillin streptomycin

    https://www.bioz.com/result/penicillin streptomycin/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
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    Cell Culture:

    Article Title: VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes
    Article Snippet: .. HEK293, HeLa S3, U2OS and NIH3T3 cells were cultured in DMEM and hTERT-RPE1 cells in DME/F-12 (1:1) (Hyclone) supplemented with 10% FBS (Atlanta Biologicals), 100 U/ml penicillin G and 100 μg/ml streptomycin (Hyclone) in the presence of 5% CO2. .. The expression of GFP-Mps1∆12/13 in HeLa GFP-Mps1∆12/13 cells that were maintained of 500 μg/ml G418 (Sigma) was induced by doxycycline (dox; Sigma) at 1 μg/ml.

    Article Title: Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
    Article Snippet: .. Cell Culture The human hepatocellular carcinoma cell line HepG2 cells, human lung adenocarcinoma cell line A549 cells, human gastric adenocarcinoma cell line SGC7901 cells, and human breast carcinoma cell line MCF-7 cells were bought from the cell bank of Chinese Academy of Sciences and cultured in Dulbecco’s modified Eagle medium (DMEM) (Hyclone, South Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin solution (Hyclone, South Logan, UT, USA). ..

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Article Title: C18H17NO6 Inhibits Invasion and Migration of Human MNNG Osteosarcoma Cells via the PI3K/AKT Signaling Pathway
    Article Snippet: .. MNNG cells were cultured at 37°C in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin (HyClone, USA) in a cell incubator containing 5% CO2 . .. When the cells grew to 80–90% confluence, digestion was terminated with 0.25% trypsin (HyClone, USA) after 2 min. After centrifugation at 600 rpm for 5 min, the cells were collected and subcultured according to experimental protocols.

    Modification:

    Article Title: Xanthones from the Bark of Garcinia xanthochymus and the Mechanism of Induced Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells via the Mitochondrial Pathway
    Article Snippet: .. Cell Culture The human hepatocellular carcinoma cell line HepG2 cells, human lung adenocarcinoma cell line A549 cells, human gastric adenocarcinoma cell line SGC7901 cells, and human breast carcinoma cell line MCF-7 cells were bought from the cell bank of Chinese Academy of Sciences and cultured in Dulbecco’s modified Eagle medium (DMEM) (Hyclone, South Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin solution (Hyclone, South Logan, UT, USA). ..

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Article Title: C18H17NO6 Inhibits Invasion and Migration of Human MNNG Osteosarcoma Cells via the PI3K/AKT Signaling Pathway
    Article Snippet: .. MNNG cells were cultured at 37°C in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin-streptomycin (HyClone, USA) in a cell incubator containing 5% CO2 . .. When the cells grew to 80–90% confluence, digestion was terminated with 0.25% trypsin (HyClone, USA) after 2 min. After centrifugation at 600 rpm for 5 min, the cells were collected and subcultured according to experimental protocols.

    other:

    Article Title: Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice
    Article Snippet: Reagents and Antibodies For splenocytes culture, RPMI medium supplemented with penicillin/streptomycin (Wellgene, Seoul, Korea) and 10% fetal bovine serum (Hyclone, Pittsburgh, PA, USA) were used.

    Transfection:

    Article Title: lncRNA PTAR promotes NSCLC cell proliferation, migration and invasion by sponging microRNA-101
    Article Snippet: .. Cell culture and transfection All cells were cultured in Dulbecco's Modified Eagle's medium (Hyclone; GE Healthcare) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare) and 100 U/ml penicillin/streptomycin (Hyclone; GE Healthcare). .. Phosphate-buffered saline (PBS; Hyclone; GE Healthcare) solution was used for washing cells before dissociation and diluting cells for counting.

    Size-exclusion Chromatography:

    Article Title: Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations †
    Article Snippet: The reaction of β-lactam antibiotics with PBP 2 is denoted by the following equation: E + S ↔ K s E • S → k 2 E − S ′ → k 3 E + P , where E•S is the non-covalent enzyme-antibiotic complex, E-S’ is the acyl-enzyme complex, and P the hydrolyzed antibiotic. k2 /Ks constants, which are a direct measure of the ability of an antibiotic to inhibit a PBP , were calculated from first order rates of acylation of purified, soluble PBP 2 variants by [14 C]penicillin G (Moravek, Brea, CA) as previously described ( , ). .. Graphs of PBP 2-[14 C]penicillin G complex formation versus time were obtained by incubating 27 μg of protein with 1.0 μM [14 C]penicillin G, and aliquots of ~4 μg were removed at 15 sec intervals, precipitated with 5% trichloroacetic acid, filtered over Whatman GC-A filters, and the filters were submitted to scintillation counting. .. The concentration of [14 C]penicillin G was increased to 25 and 50 μM for determination of k2 /KS values with PBP 235/02 and PBP 235/02 -A501V, respectively.

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  • 97
    GE Healthcare fetal bovine serum fbs
    Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in <t>MEM-α</t> with 10% <t>FBS</t> and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p
    Fetal Bovine Serum Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/GE Healthcare
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    97
    GE Healthcare c penicillin g
    Log-phase  H. pylori  43579 Amox s  and Amox r  cultures were incubated with [ 14 C]penicillin G and 1-ml aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed; the resulting pellets and washes were then analyzed for radioactivity in a scintillation counter. Squares, Amox s  strain; triangles, Amox r  strain. Data bars represent the means ± standard errors of the means based on five separate experiments.
    C Penicillin G, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Inhibitory effects of KIOM-MA128 on PGD 2 production and the activation of the arachidonate cascade. RBL-2H3 mast cells were seeded on a 6-well plate (5 × 10 5 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. The amounts of PGD 2 were determined as described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments. The cells were washed with 1× DPBS and lysed with cell lysis buffer. The levels of p-cPLA 2 , COX-2 and α-tubulin were determined using the procedure described in the Materials and Methods section. # p

    Article Snippet: Reagents MEM-α medium, 1 × DPBS, penicillin, streptomycin and fetal bovine serum (FBS) were purchased from GE Healthcare Life Sciences (Hyclone™, Logan, UT, USA).

    Techniques: Activation Assay, Incubation, Lysis

    Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Journal: Molecules

    Article Title: The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo

    doi: 10.3390/molecules21081015

    Figure Lengend Snippet: Effect of KIOM-MA128 on cell viability in IgE/Ag-activated RBL-2H3 mast cells. RBL-2H3 mast cells were seeded on a 96-well plate (1 × 10 4 cells/well) in MEM-α with 10% FBS and incubated overnight at 37 °C. The cells were further incubated with DNP-IgE (0.1 μg) for 24 h and then treated with KIOM-MA128 (0–2000 μg/mL). After 1 h, they were stimulated with DNP-Ag (0.1 μg/mL) for 4 h. Cell viability was determined using the procedure described in the Materials and Methods section. The data represent the mean ± SD values of three independent experiments.

    Article Snippet: Reagents MEM-α medium, 1 × DPBS, penicillin, streptomycin and fetal bovine serum (FBS) were purchased from GE Healthcare Life Sciences (Hyclone™, Logan, UT, USA).

    Techniques: Incubation

    Log-phase  H. pylori  43579 Amox s  and Amox r  cultures were incubated with [ 14 C]penicillin G and 1-ml aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed; the resulting pellets and washes were then analyzed for radioactivity in a scintillation counter. Squares, Amox s  strain; triangles, Amox r  strain. Data bars represent the means ± standard errors of the means based on five separate experiments.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Characterization of an In Vitro-Selected Amoxicillin-Resistant Strain of Helicobacter pylori

    doi:

    Figure Lengend Snippet: Log-phase H. pylori 43579 Amox s and Amox r cultures were incubated with [ 14 C]penicillin G and 1-ml aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed; the resulting pellets and washes were then analyzed for radioactivity in a scintillation counter. Squares, Amox s strain; triangles, Amox r strain. Data bars represent the means ± standard errors of the means based on five separate experiments.

    Article Snippet: Log-phase Amoxs and Amoxr cultures were concentrated to 3 × 109 to 5 × 109 bacteria/ml and then were incubated with 6.7 μg of [14 C]penicillin G (1 μCi/ml; Amersham) per ml for 1 h. One-milliliter aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed four times in PBS.

    Techniques: Incubation, Radioactivity

    Log-phase  H. pylori  43579 Amox r  cultures were preincubated with either PBS or CCCP for 15 min prior to incubation with [ 14 C]penicillin G for 1 h. One-milliliter aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed; the resulting pellets and washes were then analyzed for radioactivity in a scintillation counter. Triangles, Amox r  strain without CCCP treatment; circles, Amox r  strain with CCCP treatment. Data bars represent the means ± standard errors of the means based on three separate experiments.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Characterization of an In Vitro-Selected Amoxicillin-Resistant Strain of Helicobacter pylori

    doi:

    Figure Lengend Snippet: Log-phase H. pylori 43579 Amox r cultures were preincubated with either PBS or CCCP for 15 min prior to incubation with [ 14 C]penicillin G for 1 h. One-milliliter aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed; the resulting pellets and washes were then analyzed for radioactivity in a scintillation counter. Triangles, Amox r strain without CCCP treatment; circles, Amox r strain with CCCP treatment. Data bars represent the means ± standard errors of the means based on three separate experiments.

    Article Snippet: Log-phase Amoxs and Amoxr cultures were concentrated to 3 × 109 to 5 × 109 bacteria/ml and then were incubated with 6.7 μg of [14 C]penicillin G (1 μCi/ml; Amersham) per ml for 1 h. One-milliliter aliquots were taken at 5, 10, 30, and 60 min, centrifuged, and washed four times in PBS.

    Techniques: Incubation, Radioactivity

    PTP-PEST S571 phosphorylation mediates v-H-Ras-regulated cell focal adhesion, migration, and invasion. (A) PEST-PEST +/+ and PEST-PEST −/− cells with or without stable expression of WT FLAG-PTP-PEST or the FLAG-PTP-PEST S571A mutant. Western

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: PTP-PEST S571 phosphorylation mediates v-H-Ras-regulated cell focal adhesion, migration, and invasion. (A) PEST-PEST +/+ and PEST-PEST −/− cells with or without stable expression of WT FLAG-PTP-PEST or the FLAG-PTP-PEST S571A mutant. Western

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: Migration, Expressing, Mutagenesis, Western Blot

    PTP-PEST S571 phosphorylation promotes the interaction between PTP-PEST and FAK and subsequent dephosphorylation of FAK Y397. Western blotting analyses were performed with the indicated antibodies. (A) Cell lysates were prepared from PEST-PEST −/−

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: PTP-PEST S571 phosphorylation promotes the interaction between PTP-PEST and FAK and subsequent dephosphorylation of FAK Y397. Western blotting analyses were performed with the indicated antibodies. (A) Cell lysates were prepared from PEST-PEST −/−

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: De-Phosphorylation Assay, Western Blot

    cis-trans isomerization of PTP-PEST by PIN1 is required for the interaction between PTP-PEST and FAK and subsequent FAK dephosphorylation. Western blotting and immunoprecipitation analyses were performed with the indicated antibodies. (A and B) U251 cells

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: cis-trans isomerization of PTP-PEST by PIN1 is required for the interaction between PTP-PEST and FAK and subsequent FAK dephosphorylation. Western blotting and immunoprecipitation analyses were performed with the indicated antibodies. (A and B) U251 cells

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: De-Phosphorylation Assay, Western Blot, Immunoprecipitation

    A proposed mechanism for activated Ras-induced and ERK1/2- and PIN1-dependent FAK Y397 dephosphorylation by PTP-PEST. Activated Ras results in ERK1/2-dependent phosphorylation of PTP-PEST S571 and FAK S910 and subsequent recruitment of PIN1 for cis-trans

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: A proposed mechanism for activated Ras-induced and ERK1/2- and PIN1-dependent FAK Y397 dephosphorylation by PTP-PEST. Activated Ras results in ERK1/2-dependent phosphorylation of PTP-PEST S571 and FAK S910 and subsequent recruitment of PIN1 for cis-trans

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: De-Phosphorylation Assay

    PTP-PEST and FAK bind to PIN1 in a mutually independent manner. Western blotting (WB) and immunoprecipitation (IP) analyses were performed with the indicated antibodies. Tubulin was used as a loading control. (A) FAK +/+ and FAK −/− cells

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: PTP-PEST and FAK bind to PIN1 in a mutually independent manner. Western blotting (WB) and immunoprecipitation (IP) analyses were performed with the indicated antibodies. Tubulin was used as a loading control. (A) FAK +/+ and FAK −/− cells

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: Western Blot, Immunoprecipitation

    ERK1/2 phosphorylate PTP-PEST and are required for PIN1 to bind to PTP-PEST. Western blotting analyses were performed with the indicated antibodies. (A) A vector expressing FLAG-PTP-PEST was cotransfected with or without a vector expressing v-H-Ras in

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: ERK1/2 phosphorylate PTP-PEST and are required for PIN1 to bind to PTP-PEST. Western blotting analyses were performed with the indicated antibodies. (A) A vector expressing FLAG-PTP-PEST was cotransfected with or without a vector expressing v-H-Ras in

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: Western Blot, Plasmid Preparation, Expressing

    ERK1/2 phosphorylate PTP-PEST at S571, which is required for recruiting PIN1. (A) Purified His-PTP-PEST was phosphorylated by ERK2 in vitro and was analyzed by mass spectrometry. Mass spectrometric analysis of a tryptic fragment at m/z 1050.2 matched

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: ERK1/2 phosphorylate PTP-PEST at S571, which is required for recruiting PIN1. (A) Purified His-PTP-PEST was phosphorylated by ERK2 in vitro and was analyzed by mass spectrometry. Mass spectrometric analysis of a tryptic fragment at m/z 1050.2 matched

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: Purification, In Vitro, Mass Spectrometry

    PTP-PEST S571 phosphorylation promotes v-H-Ras-regulated metastasis. (A) v-H-Ras was stably expressed in PEST-PEST +/+ and PEST-PEST −/− cells with or without stable expression of FLAG-PTP-PEST or the FLAG-PTP-PEST S571A mutant. Western

    Journal: Molecular and Cellular Biology

    Article Title: Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST ▿

    doi: 10.1128/MCB.05547-11

    Figure Lengend Snippet: PTP-PEST S571 phosphorylation promotes v-H-Ras-regulated metastasis. (A) v-H-Ras was stably expressed in PEST-PEST +/+ and PEST-PEST −/− cells with or without stable expression of FLAG-PTP-PEST or the FLAG-PTP-PEST S571A mutant. Western

    Article Snippet: FAK+/+ , FAK−/− , PTP-PEST+/+ , and PTP-PEST−/− fibroblasts, U251 glioblastoma cells, and 293T cells were all maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% bovine calf serum (HyClone, Logan, UT).

    Techniques: Stable Transfection, Expressing, Mutagenesis, Western Blot

    Ras-induced FAK Dephosphorylation is Mediated by PTP-PEST

    Journal: Molecular cell

    Article Title: FAK phosphorylation by ERK primes Ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST

    doi: 10.1016/j.molcel.2009.06.013

    Figure Lengend Snippet: Ras-induced FAK Dephosphorylation is Mediated by PTP-PEST

    Article Snippet: NIH3T3 mouse fibroblasts, NIH3T3-v-H-Ras cells, NIH3T3 cells expressing v-H-Ras (Tet-off), 3Y1 rat fibroblasts, 3Y1-v-H-Ras cells, MCF 10A cells, BT549 cells, U87 cells, rat thyroid epithelial cells transformed by a temperature-sensitive mutant of the Kirsten murine sarcoma virus , MK14 cells, PIN1 +/+ , PIN1 −/− , FAK −/− , and PTP-PEST −/− fibroblasts, and 293T cells were all maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (HyClone, Logan, UT).

    Techniques: De-Phosphorylation Assay

    FAK Phosphorylation at S910 Results in Binding of PIN1 and PTP-PEST to FAK at Lamellipodia and FAK Dephosphorylation at Y397

    Journal: Molecular cell

    Article Title: FAK phosphorylation by ERK primes Ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST

    doi: 10.1016/j.molcel.2009.06.013

    Figure Lengend Snippet: FAK Phosphorylation at S910 Results in Binding of PIN1 and PTP-PEST to FAK at Lamellipodia and FAK Dephosphorylation at Y397

    Article Snippet: NIH3T3 mouse fibroblasts, NIH3T3-v-H-Ras cells, NIH3T3 cells expressing v-H-Ras (Tet-off), 3Y1 rat fibroblasts, 3Y1-v-H-Ras cells, MCF 10A cells, BT549 cells, U87 cells, rat thyroid epithelial cells transformed by a temperature-sensitive mutant of the Kirsten murine sarcoma virus , MK14 cells, PIN1 +/+ , PIN1 −/− , FAK −/− , and PTP-PEST −/− fibroblasts, and 293T cells were all maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (HyClone, Logan, UT).

    Techniques: Binding Assay, De-Phosphorylation Assay

    Y397 Dephosphorylation and Inhibition of FAK Mediated by ERK, PIN1, and PTP-PEST Promotes Ras-induced Cell Migration, Invasion, and Metastasis

    Journal: Molecular cell

    Article Title: FAK phosphorylation by ERK primes Ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST

    doi: 10.1016/j.molcel.2009.06.013

    Figure Lengend Snippet: Y397 Dephosphorylation and Inhibition of FAK Mediated by ERK, PIN1, and PTP-PEST Promotes Ras-induced Cell Migration, Invasion, and Metastasis

    Article Snippet: NIH3T3 mouse fibroblasts, NIH3T3-v-H-Ras cells, NIH3T3 cells expressing v-H-Ras (Tet-off), 3Y1 rat fibroblasts, 3Y1-v-H-Ras cells, MCF 10A cells, BT549 cells, U87 cells, rat thyroid epithelial cells transformed by a temperature-sensitive mutant of the Kirsten murine sarcoma virus , MK14 cells, PIN1 +/+ , PIN1 −/− , FAK −/− , and PTP-PEST −/− fibroblasts, and 293T cells were all maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (HyClone, Logan, UT).

    Techniques: De-Phosphorylation Assay, Inhibition, Migration