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Corning Life Sciences penicillin streptomycin
Penicillin Streptomycin, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Incubation:

Article Title: Zika virus protection by a single low dose nucleoside modified mRNA vaccination
Article Snippet: .. The flask was incubated at 37°C, 5% CO2 for 1.5 hr with intermittent gentle rocking, then warmed media was added to a final concentration of 1.5% FBS, 1X GlutaMAX (Gibco) and 1X penicillin/streptomycin (Corning) in a final volume of 25 ml. .. The flask was incubated for 4 days or until cytopathic effects were visible.

Concentration Assay:

Article Title: Zika virus protection by a single low dose nucleoside modified mRNA vaccination
Article Snippet: .. The flask was incubated at 37°C, 5% CO2 for 1.5 hr with intermittent gentle rocking, then warmed media was added to a final concentration of 1.5% FBS, 1X GlutaMAX (Gibco) and 1X penicillin/streptomycin (Corning) in a final volume of 25 ml. .. The flask was incubated for 4 days or until cytopathic effects were visible.

Cell Culture:

Article Title: Copper transporters and chaperones CTR1, CTR2, ATOX1, and CCS as determinants of cisplatin sensitivity
Article Snippet: Human Embryonic Kidney 293T (HEK-293T) cells were obtained from ATCC (Manassas, VA). .. The wild type 293T and all sublines were cultured in DMEM medium (HyClone, Logan, UT) supplemented with 10% fetal bovine serum (Lonza, Basel, Switzerland), 1 mM sodium pyruvate (Lonza, Basel, Switzerland), 2 mM glutamine (Thermo Scientific, Waltham, MA) and 1X penicillin/streptomycin (Corning Life Sciences, Corning, NY). ..

Article Title: Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery
Article Snippet: .. Cell culture and virus infections NIH 3T12 murine fibroblasts (ATCC, CCL-164) were maintained in Dulbecco’s modified Eagle’s medium, DMEM (Corning, 11013CM) supplemented with 10% heat inactivated fetal bovine serum (FBS) and 1X penicillin/streptomycin (pen/strep; Corning, 30002CI). .. HE2.1 B cells (generated by Dr. Craig Forrest, provided by Dr. Laurie Krug) were maintained in RPMI 1640 medium (Corning, MT10040CM) supplemented with 10% FBS, 1X pen/strep (Corning, 30002CI), and 50μM 2-mercaptoethanol.

Article Title: Inhibition of Carbonyl Reductase 1 Enhances Metastasis of Head and Neck Squamous Cell Carcinoma through β-catenin-Mediated Epithelial-Mesenchymal Transition
Article Snippet: .. YD8, SNU-1041, and YD10B were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Corning, Manassas, VA, USA), supplemented with 10% fetal bovine serum (FBS; Corning) and 1% penicillin‑streptomycin (Corning). ..

Modification:

Article Title: Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery
Article Snippet: .. Cell culture and virus infections NIH 3T12 murine fibroblasts (ATCC, CCL-164) were maintained in Dulbecco’s modified Eagle’s medium, DMEM (Corning, 11013CM) supplemented with 10% heat inactivated fetal bovine serum (FBS) and 1X penicillin/streptomycin (pen/strep; Corning, 30002CI). .. HE2.1 B cells (generated by Dr. Craig Forrest, provided by Dr. Laurie Krug) were maintained in RPMI 1640 medium (Corning, MT10040CM) supplemented with 10% FBS, 1X pen/strep (Corning, 30002CI), and 50μM 2-mercaptoethanol.

Article Title: A Self-Healing Hierarchical Fiber Hydrogel That Mimics ECM Structure
Article Snippet: Epidermal growth factor (EGF) was purchased from Beijing Biosynthesis Biotechnology Co. Ltd. (Beijing, China). .. Fetal bovine serum (FBS), phosphate buffer solution (PBS, pH 7.4), Dulbecco’s modified Eagle’s medium (DMEM), and 1% penicillin‑streptomycin solution were purchased from Corning (Corning, NY, USA). ..

Imaging:

Article Title: Live imaging reveals hub cell assembly and compaction dynamics during morphogenesis of the Drosophila testis niche
Article Snippet: .. In 100 uL increments, 500ul of imaging media was carefully added, which consisted of 15% FBS (Gibco 10082) and 0.5X penicillin/streptomycin (Corning 30-002-CI) in Schneider’s Insect Media (Gibco 21720-024) – . .. The stock imaging media was prepared ahead of time, kept at 4°C, and used within two weeks.

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  • 86
    Corning Life Sciences r10
    AHR nucleotransloaction in MSCs treated with 1MT and AHR agonists (A, B) MSCs were plated onto glass coverslips and allowed to adhere overnight. Media was aspirated and replaced with <t>R10</t> or the indicated drug. Drugs were left on cells for 5h, after which cells were fixed and AHR was visualized via immunofluorescence; DAPI was used to visualize nuclei. Isotype-control was a non-specific murine-derived IgG. TCDD concentration was 10 nM. Concentrations of D-MT, L-MT, R-MT all at 1mM. These results (A, B) from an experiment with one MSC sample, which was replicated four times with independent MSC samples. All images were taken using a confocal microscope with the same exposure settings. (C) The bar graph represents the quantified results of nucleotranslocation, as observed via immunofluorsence. The Leica LASX software package was utilized to delimit regions of interest, defined by the DAPI-visualized nucleus. From these regions, the signal of Alexa488 was computed and normalized per μm 2 . These data are the cumulative average of three experiments using independent MSC samples, each with an average of twelve enumerations per high-power field. Statistical test performed was one-way ANOVA, P=0.003.
    R10, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r10/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r10 - by Bioz Stars, 2021-03
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    86
    Corning Life Sciences matrigel solution
    RUNX1-ETO Induction Leads to Increased Survival and a Reversible Growth Arrest (A) RUNX1-ETO induction causes a reversible block in colony-forming ability. Top: diagram depicting the experimental strategy. EB cultures were treated with 3, 5, or 10 ng/mL Dox for 7 days, and suspension cells were subsequently plated in methylcellulose for CFU assays in the presence or the absence of Dox. Below: CFU assay of day 31 progenitors from treated EB cultures (at day 24 for 7 days), plated in methylcellulose in the presence (light gray) or absence (dark gray) of Dox. Data are from three independent biologic replicates using two clones. CFU assays were conducted in triplicate, with 3,000 cells plated per well. Error bars represent the standard error of the mean (SEM). Gray asterisk: multiple t test, statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each row was analyzed individually, without assuming a consistent SD. Black asterisk: two-way ANOVA, statistical significance determined using Dunnett’s multiple comparison test. (B) Induction of RUNX1-ETO at low levels (3 and 5 ng/mL Dox) enhances the survival of a subset of progenitor cells compared with uninduced cells, without increasing proliferative capacity. Left: schematic of the replating assays. EB cultures were treated at different stages of hematopoietic differentiation with 0, 3, 5, or 10 ng/mL Dox for 7 days. Floating progenitors were harvested and plated on <t>Matrigel-coated</t> wells at 2 × 10 5 cells/well in the corresponding Dox concentration and were serially passaged each week. Right: cell count of live cells × 10 5 during replating assays of hematopoietic progenitors from day 29 cultures previously treated with Dox for 7 days (from day 22), showing one representative of three biological replicates. Cell growth was measured at the indicated times. (C) RUNX1-ETO expressed at low levels allows survival of cells until day 87. Bright-field images of hematopoietic progenitors from replating assays at day 87 of differentiation that are uninduced (left) or treated with 3 ng/mL Dox from day 22 onward (right). Images are taken using the same magnification.
    Matrigel Solution, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/matrigel solution/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    matrigel solution - by Bioz Stars, 2021-03
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    86
    Corning Life Sciences streptomycin
    RUNX1-ETO Induction Leads to Increased Survival and a Reversible Growth Arrest (A) RUNX1-ETO induction causes a reversible block in colony-forming ability. Top: diagram depicting the experimental strategy. EB cultures were treated with 3, 5, or 10 ng/mL Dox for 7 days, and suspension cells were subsequently plated in methylcellulose for CFU assays in the presence or the absence of Dox. Below: CFU assay of day 31 progenitors from treated EB cultures (at day 24 for 7 days), plated in methylcellulose in the presence (light gray) or absence (dark gray) of Dox. Data are from three independent biologic replicates using two clones. CFU assays were conducted in triplicate, with 3,000 cells plated per well. Error bars represent the standard error of the mean (SEM). Gray asterisk: multiple t test, statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each row was analyzed individually, without assuming a consistent SD. Black asterisk: two-way ANOVA, statistical significance determined using Dunnett’s multiple comparison test. (B) Induction of RUNX1-ETO at low levels (3 and 5 ng/mL Dox) enhances the survival of a subset of progenitor cells compared with uninduced cells, without increasing proliferative capacity. Left: schematic of the replating assays. EB cultures were treated at different stages of hematopoietic differentiation with 0, 3, 5, or 10 ng/mL Dox for 7 days. Floating progenitors were harvested and plated on <t>Matrigel-coated</t> wells at 2 × 10 5 cells/well in the corresponding Dox concentration and were serially passaged each week. Right: cell count of live cells × 10 5 during replating assays of hematopoietic progenitors from day 29 cultures previously treated with Dox for 7 days (from day 22), showing one representative of three biological replicates. Cell growth was measured at the indicated times. (C) RUNX1-ETO expressed at low levels allows survival of cells until day 87. Bright-field images of hematopoietic progenitors from replating assays at day 87 of differentiation that are uninduced (left) or treated with 3 ng/mL Dox from day 22 onward (right). Images are taken using the same magnification.
    Streptomycin, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptomycin/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptomycin - by Bioz Stars, 2021-03
    86/100 stars
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    86
    Corning Life Sciences g418
    RUNX1-ETO Induction Leads to Increased Survival and a Reversible Growth Arrest (A) RUNX1-ETO induction causes a reversible block in colony-forming ability. Top: diagram depicting the experimental strategy. EB cultures were treated with 3, 5, or 10 ng/mL Dox for 7 days, and suspension cells were subsequently plated in methylcellulose for CFU assays in the presence or the absence of Dox. Below: CFU assay of day 31 progenitors from treated EB cultures (at day 24 for 7 days), plated in methylcellulose in the presence (light gray) or absence (dark gray) of Dox. Data are from three independent biologic replicates using two clones. CFU assays were conducted in triplicate, with 3,000 cells plated per well. Error bars represent the standard error of the mean (SEM). Gray asterisk: multiple t test, statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each row was analyzed individually, without assuming a consistent SD. Black asterisk: two-way ANOVA, statistical significance determined using Dunnett’s multiple comparison test. (B) Induction of RUNX1-ETO at low levels (3 and 5 ng/mL Dox) enhances the survival of a subset of progenitor cells compared with uninduced cells, without increasing proliferative capacity. Left: schematic of the replating assays. EB cultures were treated at different stages of hematopoietic differentiation with 0, 3, 5, or 10 ng/mL Dox for 7 days. Floating progenitors were harvested and plated on <t>Matrigel-coated</t> wells at 2 × 10 5 cells/well in the corresponding Dox concentration and were serially passaged each week. Right: cell count of live cells × 10 5 during replating assays of hematopoietic progenitors from day 29 cultures previously treated with Dox for 7 days (from day 22), showing one representative of three biological replicates. Cell growth was measured at the indicated times. (C) RUNX1-ETO expressed at low levels allows survival of cells until day 87. Bright-field images of hematopoietic progenitors from replating assays at day 87 of differentiation that are uninduced (left) or treated with 3 ng/mL Dox from day 22 onward (right). Images are taken using the same magnification.
    G418, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g418/product/Corning Life Sciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    g418 - by Bioz Stars, 2021-03
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    Image Search Results


    AHR nucleotransloaction in MSCs treated with 1MT and AHR agonists (A, B) MSCs were plated onto glass coverslips and allowed to adhere overnight. Media was aspirated and replaced with R10 or the indicated drug. Drugs were left on cells for 5h, after which cells were fixed and AHR was visualized via immunofluorescence; DAPI was used to visualize nuclei. Isotype-control was a non-specific murine-derived IgG. TCDD concentration was 10 nM. Concentrations of D-MT, L-MT, R-MT all at 1mM. These results (A, B) from an experiment with one MSC sample, which was replicated four times with independent MSC samples. All images were taken using a confocal microscope with the same exposure settings. (C) The bar graph represents the quantified results of nucleotranslocation, as observed via immunofluorsence. The Leica LASX software package was utilized to delimit regions of interest, defined by the DAPI-visualized nucleus. From these regions, the signal of Alexa488 was computed and normalized per μm 2 . These data are the cumulative average of three experiments using independent MSC samples, each with an average of twelve enumerations per high-power field. Statistical test performed was one-way ANOVA, P=0.003.

    Journal: Oncotarget

    Article Title: The IDO inhibitor 1-methyl tryptophan activates the aryl hydrocarbon receptor response in mesenchymal stromal cells

    doi: 10.18632/oncotarget.20166

    Figure Lengend Snippet: AHR nucleotransloaction in MSCs treated with 1MT and AHR agonists (A, B) MSCs were plated onto glass coverslips and allowed to adhere overnight. Media was aspirated and replaced with R10 or the indicated drug. Drugs were left on cells for 5h, after which cells were fixed and AHR was visualized via immunofluorescence; DAPI was used to visualize nuclei. Isotype-control was a non-specific murine-derived IgG. TCDD concentration was 10 nM. Concentrations of D-MT, L-MT, R-MT all at 1mM. These results (A, B) from an experiment with one MSC sample, which was replicated four times with independent MSC samples. All images were taken using a confocal microscope with the same exposure settings. (C) The bar graph represents the quantified results of nucleotranslocation, as observed via immunofluorsence. The Leica LASX software package was utilized to delimit regions of interest, defined by the DAPI-visualized nucleus. From these regions, the signal of Alexa488 was computed and normalized per μm 2 . These data are the cumulative average of three experiments using independent MSC samples, each with an average of twelve enumerations per high-power field. Statistical test performed was one-way ANOVA, P=0.003.

    Article Snippet: Although culture-expanded in α-MEM, all subsequent tissue culture experimental work was performed in R10 (RPMI 1640 with L-glutamate plus 100 U/ml penicillin/streptomycin, and 10% fetal calf serum) (Corning International, Corning, NY).

    Techniques: Immunofluorescence, Derivative Assay, Concentration Assay, Microscopy, Software

    RNA-seq analysis of 1MT and TCDD treated MSCs (A) MSC samples (N=5) were cultured for 24h in vitro in the presence of R-MT (1 mM), TCDD (10 nM), or R10 vehicle (NoRx), and analyzed via mRNA-Seq. eat map displaying the union of all differentially-expressed genes (DEGs) found between control vehicle treated cells (NoRx) and TCDD treated cells or R-MT treated cells. DEGs were defined as +/−2-fold change and FDR

    Journal: Oncotarget

    Article Title: The IDO inhibitor 1-methyl tryptophan activates the aryl hydrocarbon receptor response in mesenchymal stromal cells

    doi: 10.18632/oncotarget.20166

    Figure Lengend Snippet: RNA-seq analysis of 1MT and TCDD treated MSCs (A) MSC samples (N=5) were cultured for 24h in vitro in the presence of R-MT (1 mM), TCDD (10 nM), or R10 vehicle (NoRx), and analyzed via mRNA-Seq. eat map displaying the union of all differentially-expressed genes (DEGs) found between control vehicle treated cells (NoRx) and TCDD treated cells or R-MT treated cells. DEGs were defined as +/−2-fold change and FDR

    Article Snippet: Although culture-expanded in α-MEM, all subsequent tissue culture experimental work was performed in R10 (RPMI 1640 with L-glutamate plus 100 U/ml penicillin/streptomycin, and 10% fetal calf serum) (Corning International, Corning, NY).

    Techniques: RNA Sequencing Assay, Cell Culture, In Vitro

    RUNX1-ETO Induction Leads to Increased Survival and a Reversible Growth Arrest (A) RUNX1-ETO induction causes a reversible block in colony-forming ability. Top: diagram depicting the experimental strategy. EB cultures were treated with 3, 5, or 10 ng/mL Dox for 7 days, and suspension cells were subsequently plated in methylcellulose for CFU assays in the presence or the absence of Dox. Below: CFU assay of day 31 progenitors from treated EB cultures (at day 24 for 7 days), plated in methylcellulose in the presence (light gray) or absence (dark gray) of Dox. Data are from three independent biologic replicates using two clones. CFU assays were conducted in triplicate, with 3,000 cells plated per well. Error bars represent the standard error of the mean (SEM). Gray asterisk: multiple t test, statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each row was analyzed individually, without assuming a consistent SD. Black asterisk: two-way ANOVA, statistical significance determined using Dunnett’s multiple comparison test. (B) Induction of RUNX1-ETO at low levels (3 and 5 ng/mL Dox) enhances the survival of a subset of progenitor cells compared with uninduced cells, without increasing proliferative capacity. Left: schematic of the replating assays. EB cultures were treated at different stages of hematopoietic differentiation with 0, 3, 5, or 10 ng/mL Dox for 7 days. Floating progenitors were harvested and plated on Matrigel-coated wells at 2 × 10 5 cells/well in the corresponding Dox concentration and were serially passaged each week. Right: cell count of live cells × 10 5 during replating assays of hematopoietic progenitors from day 29 cultures previously treated with Dox for 7 days (from day 22), showing one representative of three biological replicates. Cell growth was measured at the indicated times. (C) RUNX1-ETO expressed at low levels allows survival of cells until day 87. Bright-field images of hematopoietic progenitors from replating assays at day 87 of differentiation that are uninduced (left) or treated with 3 ng/mL Dox from day 22 onward (right). Images are taken using the same magnification.

    Journal: Cell Reports

    Article Title: Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells

    doi: 10.1016/j.celrep.2020.107691

    Figure Lengend Snippet: RUNX1-ETO Induction Leads to Increased Survival and a Reversible Growth Arrest (A) RUNX1-ETO induction causes a reversible block in colony-forming ability. Top: diagram depicting the experimental strategy. EB cultures were treated with 3, 5, or 10 ng/mL Dox for 7 days, and suspension cells were subsequently plated in methylcellulose for CFU assays in the presence or the absence of Dox. Below: CFU assay of day 31 progenitors from treated EB cultures (at day 24 for 7 days), plated in methylcellulose in the presence (light gray) or absence (dark gray) of Dox. Data are from three independent biologic replicates using two clones. CFU assays were conducted in triplicate, with 3,000 cells plated per well. Error bars represent the standard error of the mean (SEM). Gray asterisk: multiple t test, statistical significance determined using the Holm-Sidak method, with alpha = 0.05. Each row was analyzed individually, without assuming a consistent SD. Black asterisk: two-way ANOVA, statistical significance determined using Dunnett’s multiple comparison test. (B) Induction of RUNX1-ETO at low levels (3 and 5 ng/mL Dox) enhances the survival of a subset of progenitor cells compared with uninduced cells, without increasing proliferative capacity. Left: schematic of the replating assays. EB cultures were treated at different stages of hematopoietic differentiation with 0, 3, 5, or 10 ng/mL Dox for 7 days. Floating progenitors were harvested and plated on Matrigel-coated wells at 2 × 10 5 cells/well in the corresponding Dox concentration and were serially passaged each week. Right: cell count of live cells × 10 5 during replating assays of hematopoietic progenitors from day 29 cultures previously treated with Dox for 7 days (from day 22), showing one representative of three biological replicates. Cell growth was measured at the indicated times. (C) RUNX1-ETO expressed at low levels allows survival of cells until day 87. Bright-field images of hematopoietic progenitors from replating assays at day 87 of differentiation that are uninduced (left) or treated with 3 ng/mL Dox from day 22 onward (right). Images are taken using the same magnification.

    Article Snippet: At day 8, 20–30 EBs/well were transferred onto 6-well adherent plates pre-coated with Matrigel solution (IMDM with 1x Pen/Strep and 1:200 Corning® Matrigel® Growth Factor Reduced phenol red-free).

    Techniques: Blocking Assay, Colony-forming Unit Assay, Clone Assay, Concentration Assay, Cell Counting