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Cellgro penicillin streptomycin
Penicillin Streptomycin, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/penicillin streptomycin/product/Cellgro
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
penicillin streptomycin - by Bioz Stars, 2021-03
86/100 stars

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Plaque Assay:

Article Title: Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure
Article Snippet: Forward primer (0.6 μM): 5′—CCA GTT CCA GCA ATT ACA ATA CAT ACA—3′ Reverse primer (0.6 μM): 5′—GCA CCG TGG TCA GCA TAA GGA—3′ Probe (0.2 μM): 6FAM—CAA TAC CTT AAC CCC C—MGBNFQ .. Plaque Assay A 1:10 dilution series ranging from 10−1 to 10−6 was prepared in 1X Plaque Assay Media [1X MEM (Corning Cellgro, Manassas, VA) containing 5% heat-inactivated fetal calf serum (Life Technologies, Grand Island, NY) and 1X Penicillin/Streptomycin (Corning Cellgro, Manassas, VA)] for each sample for plaque titration. .. In addition, a positive control sample consisting of MARV/Angola at a known concentration was similarly diluted in Plaque Assay Media.

Titration:

Article Title: Dose Response of MARV/Angola Infection in Cynomolgus Macaques following IM or Aerosol Exposure
Article Snippet: Forward primer (0.6 μM): 5′—CCA GTT CCA GCA ATT ACA ATA CAT ACA—3′ Reverse primer (0.6 μM): 5′—GCA CCG TGG TCA GCA TAA GGA—3′ Probe (0.2 μM): 6FAM—CAA TAC CTT AAC CCC C—MGBNFQ .. Plaque Assay A 1:10 dilution series ranging from 10−1 to 10−6 was prepared in 1X Plaque Assay Media [1X MEM (Corning Cellgro, Manassas, VA) containing 5% heat-inactivated fetal calf serum (Life Technologies, Grand Island, NY) and 1X Penicillin/Streptomycin (Corning Cellgro, Manassas, VA)] for each sample for plaque titration. .. In addition, a positive control sample consisting of MARV/Angola at a known concentration was similarly diluted in Plaque Assay Media.

Cell Culture:

Article Title: Time-course proteomics dataset monitoring HeLa cells subjected to DTT induced endoplasmic reticulum stress
Article Snippet: The treatment periods were 0, 0.5, 1, 2, 8, 16, 24, and 30 h. A label-free mass spectrometry approach was used for quantification of the proteome at each time point ( ). .. 2.2 HeLa cells and growth condition HeLa cells were cultured in Dulbecco׳s Modified Eagle׳s Medium (DMEM, Sigma-Aldrich, D5796) with 10% fetal bovine serum (Atlanta Biologicals, S11150) and 1×penicillin–streptomycin solution (Corning Cellgro, 30-002-CI) at 37 °C and 5% CO2 . .. When the cells were growing to approximately 70% confluency, the small molecule redox reagent DTT (Sigma-Aldrich, D9779) was added into each cell culture plate to make a 2.5 mM final concentration to induce ER stress.

Article Title: Neuregulin 1 promotes excitatory synapse development specifically in GABAergic interneurons
Article Snippet: .. Dissociated neurons were prepared from E18 Sprague-Dawley rat or GAD67-GFP+/− mouse embryos and cultured in the neurobasal medium (21103-049, Gibco, Carlsbad, CA) supplemented with 1X B27 (17504-044, Gibco), 600 μM L-Glutamine (25-005-CI, Cellgro, Lawrence, KS) and 1X penicillin-streptomycin (30-003-CI, Cellgro). .. For low density culture, 2.5 × 104 cells were seeded on a glass coverslip (1.8 cm in diameter, 12-545-84, Fisher Scientific, Suwanee, GA) coated for 12 hr with 1μg/ml poly-L-lysine (P2636, Sigma, St. Louis, MO).

Modification:

Article Title: Time-course proteomics dataset monitoring HeLa cells subjected to DTT induced endoplasmic reticulum stress
Article Snippet: The treatment periods were 0, 0.5, 1, 2, 8, 16, 24, and 30 h. A label-free mass spectrometry approach was used for quantification of the proteome at each time point ( ). .. 2.2 HeLa cells and growth condition HeLa cells were cultured in Dulbecco׳s Modified Eagle׳s Medium (DMEM, Sigma-Aldrich, D5796) with 10% fetal bovine serum (Atlanta Biologicals, S11150) and 1×penicillin–streptomycin solution (Corning Cellgro, 30-002-CI) at 37 °C and 5% CO2 . .. When the cells were growing to approximately 70% confluency, the small molecule redox reagent DTT (Sigma-Aldrich, D9779) was added into each cell culture plate to make a 2.5 mM final concentration to induce ER stress.

other:

Article Title: Identification and Functional Distribution of Intracellular Ca2+ Channels in Mouse Lacrimal Gland Acinar Cells
Article Snippet: Culture Medium and Materials Dulbecco’s minimium essential medium without sodium pyruvate (DMEM) (Hyclone, Logan, UT), 100X penicillin-streptomycin (p/s, Cellgro, Herndon, VA), heat inactivated bovine growth serum (BGS) (Hyclone, Logan, UT), Mouse laminin (Sigma, St. Louis, MO), soybean trypsin inhibitor (STI)_(Sigma, St. Louis, MO), EGTA (Sigma, St. Louis, MO), Collagenase blend (Sigma, St. Louis, MO), 0.25% trypsin (Hyclone, Logan, UT), and bovine serum albumin, fraction V (Sigma, St. Louis, MO) were used in isolating, culturing, and for glass coverslips.

Infection:

Article Title: Modulation of Cell Adhesion and Migration by the Histone Methyltransferase Subunit mDpy-30 and Its Interacting Proteins
Article Snippet: All siRNAs were used at a concentration of 20 nM. .. Generation and infection of lentiviral particles HEK293T cells (ATCC, Rockville, MD, USA) were maintained in DMEM medium containing 10% fetal bovine serum and 1X penicillin-streptomycin (Cellgro) until they reached 70% confluence. .. For transfection of cells grown on one 15 cm dish, pMD2G (envelop plasmid, 7.9 µg), psPAX2 (packaging plasmid, 14.6 µg), and either pWPI (vector plasmid, 22.5 µg) or pWPI-mDpy-30 (mDpy-30 expressing plasmid, 22.5 µg) were mixed in a 50 ml conical tune, followed by the addition of 0.1X TE (1 mM Tris, 0.1 mM EDTA, pH 8.8, 660 µl), water (350 µl), and 2.5 M calcium chloride (113 µl).

Article Title: Identification of a Deubiquitinating Enzyme as a Novel AGS3-Interacting Protein
Article Snippet: All siRNAs were used at a concentration of 30 nM. .. Generation and infection of lentiviral particles HEK293T cells were maintained in DMEM medium containing 10% fetal bovine serum and 1X penicillin-streptomycin (Cellgro) until they reached 70% confluence. .. For transfection of cells grown on one 10 cm dish, pMD2G (envelop plasmid, 2.9 µg), psPAX2 (packaging plasmid, 5.4 µg), and either pLVX-shRNA1 (vector plasmid, 8.3 µg) or pLVX-shRNA1 containing USP9x shRNA2 or 3 (8.3 µg) were mixed in a 50 ml conical tube, followed by the addition of 0.1X TE (1 mM Tris, 0.1 mM EDTA, pH 8.8, 242 µl), water (128 µl), and 2.5 M calcium chloride (41.5 µl).

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  • 86
    Cellgro fetal bovine serum fbs
    GSP induces significantly less apoptosis in p53-deficient (p53 -/- ) cells compared to p53 wild-type (p53 +/+ ) cells. Both p53 +/+ and p53 -/- fibroblast cells were cultured under identical conditions as was done in JB6 C141 cells except that the cells were treated with 15% <t>FBS</t> in <t>DMEM.</t> Cells were starved in 0.5% FBS/DMEM overnight and thereafter treated with GSP in serum-containing media for another 24 hours. Morphologic changes in p53 +/+ and p53 -/- fibroblast cells undergoing apoptosis were observed under fluorescence microscopy. Cells in panels A and G were not treated with GSP (controls), whereas increasing concentrations of GSP were added to cultures for 24 hours after overnight serum starvation in panels B–E and H–K by 20, 40, 60, and 80 µg/ml, respectively. The percentage of apoptotic cells from panel A to panel E is summarized in panel F, and the percentage of apoptotic cells from panel G to panel K is summarized in panel L. The experiment was repeated at least two times and the average percentage of apoptotic cells ± SD for each treatment group is indicated. At least 200 cells were counted in a blinded manner to score the percentage of apoptosis in each treatment group. *P
    Fetal Bovine Serum Fbs, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal bovine serum fbs/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fetal bovine serum fbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cellgro penicillin streptomycin
    GSP induces significantly less apoptosis in p53-deficient (p53 -/- ) cells compared to p53 wild-type (p53 +/+ ) cells. Both p53 +/+ and p53 -/- fibroblast cells were cultured under identical conditions as was done in JB6 C141 cells except that the cells were treated with 15% <t>FBS</t> in <t>DMEM.</t> Cells were starved in 0.5% FBS/DMEM overnight and thereafter treated with GSP in serum-containing media for another 24 hours. Morphologic changes in p53 +/+ and p53 -/- fibroblast cells undergoing apoptosis were observed under fluorescence microscopy. Cells in panels A and G were not treated with GSP (controls), whereas increasing concentrations of GSP were added to cultures for 24 hours after overnight serum starvation in panels B–E and H–K by 20, 40, 60, and 80 µg/ml, respectively. The percentage of apoptotic cells from panel A to panel E is summarized in panel F, and the percentage of apoptotic cells from panel G to panel K is summarized in panel L. The experiment was repeated at least two times and the average percentage of apoptotic cells ± SD for each treatment group is indicated. At least 200 cells were counted in a blinded manner to score the percentage of apoptosis in each treatment group. *P
    Penicillin Streptomycin, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin streptomycin/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cellgro penicillin streptomycin solution
    GSP induces significantly less apoptosis in p53-deficient (p53 -/- ) cells compared to p53 wild-type (p53 +/+ ) cells. Both p53 +/+ and p53 -/- fibroblast cells were cultured under identical conditions as was done in JB6 C141 cells except that the cells were treated with 15% <t>FBS</t> in <t>DMEM.</t> Cells were starved in 0.5% FBS/DMEM overnight and thereafter treated with GSP in serum-containing media for another 24 hours. Morphologic changes in p53 +/+ and p53 -/- fibroblast cells undergoing apoptosis were observed under fluorescence microscopy. Cells in panels A and G were not treated with GSP (controls), whereas increasing concentrations of GSP were added to cultures for 24 hours after overnight serum starvation in panels B–E and H–K by 20, 40, 60, and 80 µg/ml, respectively. The percentage of apoptotic cells from panel A to panel E is summarized in panel F, and the percentage of apoptotic cells from panel G to panel K is summarized in panel L. The experiment was repeated at least two times and the average percentage of apoptotic cells ± SD for each treatment group is indicated. At least 200 cells were counted in a blinded manner to score the percentage of apoptosis in each treatment group. *P
    Penicillin Streptomycin Solution, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin streptomycin solution/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin solution - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    GSP induces significantly less apoptosis in p53-deficient (p53 -/- ) cells compared to p53 wild-type (p53 +/+ ) cells. Both p53 +/+ and p53 -/- fibroblast cells were cultured under identical conditions as was done in JB6 C141 cells except that the cells were treated with 15% FBS in DMEM. Cells were starved in 0.5% FBS/DMEM overnight and thereafter treated with GSP in serum-containing media for another 24 hours. Morphologic changes in p53 +/+ and p53 -/- fibroblast cells undergoing apoptosis were observed under fluorescence microscopy. Cells in panels A and G were not treated with GSP (controls), whereas increasing concentrations of GSP were added to cultures for 24 hours after overnight serum starvation in panels B–E and H–K by 20, 40, 60, and 80 µg/ml, respectively. The percentage of apoptotic cells from panel A to panel E is summarized in panel F, and the percentage of apoptotic cells from panel G to panel K is summarized in panel L. The experiment was repeated at least two times and the average percentage of apoptotic cells ± SD for each treatment group is indicated. At least 200 cells were counted in a blinded manner to score the percentage of apoptosis in each treatment group. *P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Grape Seed Proanthocyanidins Induce Apoptosis through p53, Bax, and Caspase 3 Pathways 1

    doi:

    Figure Lengend Snippet: GSP induces significantly less apoptosis in p53-deficient (p53 -/- ) cells compared to p53 wild-type (p53 +/+ ) cells. Both p53 +/+ and p53 -/- fibroblast cells were cultured under identical conditions as was done in JB6 C141 cells except that the cells were treated with 15% FBS in DMEM. Cells were starved in 0.5% FBS/DMEM overnight and thereafter treated with GSP in serum-containing media for another 24 hours. Morphologic changes in p53 +/+ and p53 -/- fibroblast cells undergoing apoptosis were observed under fluorescence microscopy. Cells in panels A and G were not treated with GSP (controls), whereas increasing concentrations of GSP were added to cultures for 24 hours after overnight serum starvation in panels B–E and H–K by 20, 40, 60, and 80 µg/ml, respectively. The percentage of apoptotic cells from panel A to panel E is summarized in panel F, and the percentage of apoptotic cells from panel G to panel K is summarized in panel L. The experiment was repeated at least two times and the average percentage of apoptotic cells ± SD for each treatment group is indicated. At least 200 cells were counted in a blinded manner to score the percentage of apoptosis in each treatment group. *P

    Article Snippet: Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, fetal bovine serum (FBS), and trypsin/EDTA were purchased from CellGro (Herndon, VA).

    Techniques: Cell Culture, Fluorescence, Microscopy

    Treatment of GSP decreases the expression of the antiapoptotic proteins, Bcl-2 and Bcl-xl, and increases the expression of the pro-apoptotic protein, Bax, in JB6 C141 cells. JB6 C141 cells were starved in 0.5% FBS/DMEM overnight and then treated with GSP (20–80 µg/ml) in serum-containing media for another 24 hours. Cell lysates were prepared, and the expression of the proteins was determined by Western blot analysis using the corresponding antibodies, as detailed in the Materials and Methods section. Relative intensity of bands in each panel was determined. GSP treatment to JB6 C141 cells decreased the expressions of the antiapoptotic proteins, Bcl-2 (panel A) and Bcl-xl (panel D), whereas the expression of the pro-apoptotic protein, Bax, was increased (panel B). A representative blot is shown from three independent experiments with identical results. The ratio of Bax and Bcl-2 protein expression was determined from three separate experiments by comparing the relative intensities of protein bands and shown as a mean ± SD (panel C). β-Actin was used as an internal control to monitor equal protein loading and transfer of proteins from the gel to the membranes after stripping them and reprobing them with the actin antibody.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Grape Seed Proanthocyanidins Induce Apoptosis through p53, Bax, and Caspase 3 Pathways 1

    doi:

    Figure Lengend Snippet: Treatment of GSP decreases the expression of the antiapoptotic proteins, Bcl-2 and Bcl-xl, and increases the expression of the pro-apoptotic protein, Bax, in JB6 C141 cells. JB6 C141 cells were starved in 0.5% FBS/DMEM overnight and then treated with GSP (20–80 µg/ml) in serum-containing media for another 24 hours. Cell lysates were prepared, and the expression of the proteins was determined by Western blot analysis using the corresponding antibodies, as detailed in the Materials and Methods section. Relative intensity of bands in each panel was determined. GSP treatment to JB6 C141 cells decreased the expressions of the antiapoptotic proteins, Bcl-2 (panel A) and Bcl-xl (panel D), whereas the expression of the pro-apoptotic protein, Bax, was increased (panel B). A representative blot is shown from three independent experiments with identical results. The ratio of Bax and Bcl-2 protein expression was determined from three separate experiments by comparing the relative intensities of protein bands and shown as a mean ± SD (panel C). β-Actin was used as an internal control to monitor equal protein loading and transfer of proteins from the gel to the membranes after stripping them and reprobing them with the actin antibody.

    Article Snippet: Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, fetal bovine serum (FBS), and trypsin/EDTA were purchased from CellGro (Herndon, VA).

    Techniques: Expressing, Western Blot, Stripping Membranes