penicillin streptomycin solution  (Millipore)

 
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  • 86
    Name:
    Penicillin Streptomycin Solution Hybri Max
    Description:

    Catalog Number:
    p7539
    Price:
    None
    Applications:
    Penicillin-Streptomycin is used to supplement cell culture media to control bacterial contamination.
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    Structured Review

    Millipore penicillin streptomycin solution

    https://www.bioz.com/result/penicillin streptomycin solution/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin solution - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    other:

    Article Title: Stable centrosomal roots disentangle to allow interphase centriole independence
    Article Snippet: Cells were fused using Hybri-Max 50% 1450 polyethylene glycol solution (Sigma-Aldrich).

    Article Title: Examining Safety of Biocolourants from Fungal and Plant Sources-Examples from Cortinarius and Tapinella, Salix and Tanacetum spp. and Dyed Woollen Fabrics
    Article Snippet: Hepa-1 cells were grown in α-MEM supplemented with 1% l-glutamine, 10% endotoxin-free FBS and 1% penicillin-streptomycin solution.

    Article Title: CXCL1 contributes to IL-6 expression in osteoarthritis and rheumatoid arthritis synovial fibroblasts by CXCR2, c-Raf, MAPK, and AP-1 pathway
    Article Snippet: The SFs were maintained in complete DMEM with 10% FBS, penicillin-streptomycin solution, and 2 mM l -glutamine at 37 °C with 5% CO2.

    Article Title: Complexation with Random Methyl-β-Cyclodextrin and (2-Hydroxypropyl)-β-Cyclodextrin Promotes Chrysin Effect and Potential for Liver Fibrosis Therapy
    Article Snippet: Human Caco-2 intestinal epithelial cells were obtained from the European Collection of Cell Cultures (ECACC, UK) and grown routinely in Dulbecco’s Minimum Essential Medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acid and 1% penicillin–streptomycin solution.

    Article Title: Influence of CD4 T cells and the source of major histocompatibility complex class II-restricted peptides on cytotoxic T-cell priming by dendritic cells
    Article Snippet: Mature day 9–10 DC (4 × 106 ) were washed once in Hybri-Max® (Sigma) containing 50 µ m 2-ME and resuspended in 0·1 ml of the same medium containing 0·01–1 µg/ml of peptide as indicated in the figure legends.

    Generated:

    Article Title: Polysaccharide PCP-I isolated from Poria cocos enhances the immunogenicity and protection of an anthrax protective antigen-based vaccine
    Article Snippet: Spots corresponding to cytokine-secreting cells were counted using an AT-Spot2100 ELISPOT plate Analyzer. .. Dendritic cell (DC) activationBone marrow-derived DCs (BMDCs) were generated by cultivating bone marrow cells in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), 1% Penicillin/Streptomycin solution, 20 ng/mL mGM-CSF(R & D, Cat#415-ML-020) and 10 ng/mL mIL-4 (R & D, Cat#415-ML-020) for seven days. .. The cells were stimulated with PA 50 μg/mL + PCP-I 2 mg/mL, PA 50 μg/mL, LPS (Sigma, Cat#L4391) 2 μg/mL, or PBS for 24 h. The expression of CD11c (BD Pharmingen clone HL3, Cat#553801), MHC II (BD Pharmingen clone AF6-120.1, Cat#562823), and CD86 (BD Pharmingen clone GL1, Cat#560582) on DCs was evaluated by FACSVerse (BD).

    Cell Culture:

    Article Title: Sodium selectivity of semicircular canal duct epithelial cells
    Article Snippet: Primary culture of SCCD epithelia SCCD were dissected from neonatal Wistar rats (3-5 days), as described previously [ , ] and followed protocols in compliance with internationally recognized guidelines with approval by the Kansas State University Institutional Animal Care and Use Committee. .. Epithelial cells from SCCD, exclusive of common crus, were dispersed and seeded on Transwell permeable supports (6.5 mm diameter, Costar 3470, Corning, NY) or on glass cover slips (3 mm × 5 mm) and cultured with DMEM/F-12 medium (Invitrogen 12500-062, Carlsbad, CA) supplemented with 5% fetal bovine serum, penicillin (100 U/ml) and streptomycin (100 μg/ml) (Sigma-Aldrich #P-7539). ..

    Article Title: Elevated COUP-TFII expression in dopaminergic neurons accelerates the progression of Parkinson’s disease through mitochondrial dysfunction
    Article Snippet: .. Cell lines SH-SY5Y (CRL-2266, female-derived, ATCC) cells were cultured in DMEM/F-12 (Gibco) supplemented with fetal bovine serum (FBS) (10%, Gibco), penicillin/streptomycin (1:100, P7539, Sigma), and L-glutamine (1:100, P7513, Sigma). .. DA-like SH-SY5Y neuroblastoma cells were obtained from culturing in the differentiation media consisting of DMEM/F-12 supplemented with FBS (1%) and retinoic acid (20 μM) for 7 days.

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  • 97
    Millipore penicilin streptomycin neomycin stabilized solution
    Ratio of the quantification of growth inhibition of Chromobacterium violaceum ATCC 12472 (read at 720 nm) when compared to quantification of the production of violacein by the same bacterium (577 nm). B: 0.1% DMSO; C: 1:100 dillution of <t>penicilin-streptomycin-neomycin</t> stabilized solution (Sigma P4083).
    Penicilin Streptomycin Neomycin Stabilized Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicilin streptomycin neomycin stabilized solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicilin streptomycin neomycin stabilized solution - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Millipore dimethyl sulfoxide dmso
    Effect of GTE, lut, lut-7-g, and OA on cell viability. <t>MTT</t> assay was performed to assess the cytotoxicity of GTE, lut, lut-7-g, and OA on HUVECs. HUVECs were treated with medium only (Control, denotes as “C”), vehicle only <t>(DMSO,</t> 0.25% for GTE group and 0.1% for pure compounds group; denotes as “V”), GTE (40, 80, 120, 160 μg/mL), lut (5, 10, 20 μM), lut-7-g (5, 10, 20 μM), and OA (5, 10, 20 μM) for 24 h. The cell viability was not affected after incubation for 24 h with, ( A ) GTE at concentrations up to 160 μg/mL; and ( B ) lut, lut-7-g, and OA at concentrations up to 20 μM.
    Dimethyl Sulfoxide Dmso, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dimethyl sulfoxide dmso/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dimethyl sulfoxide dmso - by Bioz Stars, 2021-03
    97/100 stars
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    Ratio of the quantification of growth inhibition of Chromobacterium violaceum ATCC 12472 (read at 720 nm) when compared to quantification of the production of violacein by the same bacterium (577 nm). B: 0.1% DMSO; C: 1:100 dillution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Journal: Marine Drugs

    Article Title: A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides

    doi: 10.3390/md17020111

    Figure Lengend Snippet: Ratio of the quantification of growth inhibition of Chromobacterium violaceum ATCC 12472 (read at 720 nm) when compared to quantification of the production of violacein by the same bacterium (577 nm). B: 0.1% DMSO; C: 1:100 dillution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Article Snippet: Marine broths with 0.1% DMSO and 1:100 penicilin-streptomycin-neomycin stabilized solution (P4083, Sigma-Aldrich, St. Louis, MO, USA) were used as negative (B) and positive (C) controls, respectively.

    Techniques: Inhibition

    Antibiofilm dose–response activity of portoamides towards the marine bacteria Halomonas aquamarina and Pseudoalteromonas atlantica . B: 0.1% DMSO; C: 4:100 dilution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Journal: Marine Drugs

    Article Title: A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides

    doi: 10.3390/md17020111

    Figure Lengend Snippet: Antibiofilm dose–response activity of portoamides towards the marine bacteria Halomonas aquamarina and Pseudoalteromonas atlantica . B: 0.1% DMSO; C: 4:100 dilution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Article Snippet: Marine broths with 0.1% DMSO and 1:100 penicilin-streptomycin-neomycin stabilized solution (P4083, Sigma-Aldrich, St. Louis, MO, USA) were used as negative (B) and positive (C) controls, respectively.

    Techniques: Activity Assay

    Antibacterial activity of portoamides towards five biofilm-forming marine bacteria Cobetia marina , Pseudoalteromonas atlantica and Halomonas aquamarina . B: 0.1% DMSO; C: 1:100 dilution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Journal: Marine Drugs

    Article Title: A Multi-Bioassay Integrated Approach to Assess the Antifouling Potential of the Cyanobacterial Metabolites Portoamides

    doi: 10.3390/md17020111

    Figure Lengend Snippet: Antibacterial activity of portoamides towards five biofilm-forming marine bacteria Cobetia marina , Pseudoalteromonas atlantica and Halomonas aquamarina . B: 0.1% DMSO; C: 1:100 dilution of penicilin-streptomycin-neomycin stabilized solution (Sigma P4083).

    Article Snippet: Marine broths with 0.1% DMSO and 1:100 penicilin-streptomycin-neomycin stabilized solution (P4083, Sigma-Aldrich, St. Louis, MO, USA) were used as negative (B) and positive (C) controls, respectively.

    Techniques: Activity Assay

    Effect of GTE, lut, lut-7-g, and OA on cell viability. MTT assay was performed to assess the cytotoxicity of GTE, lut, lut-7-g, and OA on HUVECs. HUVECs were treated with medium only (Control, denotes as “C”), vehicle only (DMSO, 0.25% for GTE group and 0.1% for pure compounds group; denotes as “V”), GTE (40, 80, 120, 160 μg/mL), lut (5, 10, 20 μM), lut-7-g (5, 10, 20 μM), and OA (5, 10, 20 μM) for 24 h. The cell viability was not affected after incubation for 24 h with, ( A ) GTE at concentrations up to 160 μg/mL; and ( B ) lut, lut-7-g, and OA at concentrations up to 20 μM.

    Journal: Molecules

    Article Title: Glossogyne tenuifolia Extract Inhibits TNF-α-Induced Expression of Adhesion Molecules in Human Umbilical Vein Endothelial Cells via Blocking the NF-κB Signaling Pathway

    doi: 10.3390/molecules200916908

    Figure Lengend Snippet: Effect of GTE, lut, lut-7-g, and OA on cell viability. MTT assay was performed to assess the cytotoxicity of GTE, lut, lut-7-g, and OA on HUVECs. HUVECs were treated with medium only (Control, denotes as “C”), vehicle only (DMSO, 0.25% for GTE group and 0.1% for pure compounds group; denotes as “V”), GTE (40, 80, 120, 160 μg/mL), lut (5, 10, 20 μM), lut-7-g (5, 10, 20 μM), and OA (5, 10, 20 μM) for 24 h. The cell viability was not affected after incubation for 24 h with, ( A ) GTE at concentrations up to 160 μg/mL; and ( B ) lut, lut-7-g, and OA at concentrations up to 20 μM.

    Article Snippet: Penicillin, streptomycin, dimethyl sulfoxide (DMSO), and MTT were purchased from Sigma Chemicals Co. (St Louis, MO, USA).

    Techniques: MTT Assay, Incubation