penicillin streptomycin glutamine  (Thermo Fisher)


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    Name:
    Penicillin Streptomycin Glutamine 100X
    Description:
    The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram positive and gram negative bacteria Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall Streptomycin was originally purified from Streptomyces griseus It acts by binding to the 30S subunit of the bacterial ribosome leading to the inhibition of protein synthesis and death in susceptible bacteria This solution contains 10 000 units of penicillin 10 000 µg of streptomycin and 29 2 mg ml of L glutamine in a 10 mM citrate buffer for pH stability We offer a wide range of antibiotics and antimycotics in both powder and liquid formats See the complete list or find products for • Contamination control• Eukaryotic and bacterial selectionSee recommended working concentrations for selection antibiotics Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures
    Catalog Number:
    10378016
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
    Buy from Supplier


    Structured Review

    Thermo Fisher penicillin streptomycin glutamine
    The antibiotics penicillin and streptomycin are used to prevent bacterial contamination in cell cultures due to their effective combined action against gram positive and gram negative bacteria Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall Streptomycin was originally purified from Streptomyces griseus It acts by binding to the 30S subunit of the bacterial ribosome leading to the inhibition of protein synthesis and death in susceptible bacteria This solution contains 10 000 units of penicillin 10 000 µg of streptomycin and 29 2 mg ml of L glutamine in a 10 mM citrate buffer for pH stability We offer a wide range of antibiotics and antimycotics in both powder and liquid formats See the complete list or find products for • Contamination control• Eukaryotic and bacterial selectionSee recommended working concentrations for selection antibiotics Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures
    https://www.bioz.com/result/penicillin streptomycin glutamine/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin glutamine - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Fibroblast Biomarkers of Sporadic Parkinson’s Disease and LRRK2 Kinase Inhibition
    Article Snippet: Sequencing was carried out at the Harvard Medical School Translational Genomics Core (Cambridge, MA) using Nextera PCR (for LRRK2) and MiSeq (for GBA1) systems (Illumina) . .. Cell Culture PD patient and healthy subject control-derived fibroblast lines were cultured in standard medium containing DMEM (Gibco), 10 % FBS (Hyclone), and 1 % Penicillin-streptomycin (Gibco #10378-016), 0.5 % glutamine (Gibco), and 1 % non-essential amino acids (Gibco). ..

    Article Title: Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing
    Article Snippet: .. Cell and Bioink Preparation Fibroblasts (CCD-1112SK, ATCC, Manassas, VA, USA) and HPV18-positive cervical cancer cells (HeLa; KCLB, Seoul, Korea) were cultured with a medium containing 10% FBS and 1% Pen Strep in a 100-mm Petri dish (Nunclon Delta Surface, Thermo Fisher Scientific, Waltham, MA, USA). .. When cell confluency reached 80%, the cells were washed three times with PBS and treated with 2 mL of trypsin-EDTA (0.25%; Gibco) to produce single-cell units before culturing in 5% CO2 at 37 °C for 2 min. After culturing, the detached cells were collected in a 15-mL conical tube into which culture medium was added, diluted 10 times to neutralize the trypsin, and centrifuged for 3 min at 1200 rpm.

    Article Title: Type I IL-4 Receptors Selectively Activate IRS-2 to Induce Target Gene Expression in Macrophages
    Article Snippet: Murine bone marrow-derived macrophages were generated from either WT C57BL/6 (Taconic Laboratories, Germantown, NY) or γC−/− (breeding pairs obtained from Taconic and bred in-house). .. Briefly, bone marrow was isolated from femurs and tibias of four- to six-week-old female mice.. To deplete adherent stromal cells, the harvested bone marrow was cultured overnight in α-10 medium [α-Minimal Essential Medium supplemented with penicillin, streptomycin, and glutamine (BioWhittaker) and 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Frederick, MD)] with 20 ng/mL of recombinant murine macrophage colony-stimulating factor [rmM-CSF, R & D Systems, Minneapolis, MN)]. .. Nonadherent cells were collected, red blood cell lysis was performed, and nonadherent mononuclear cellswere plated and cultured for ten days in the presence of 20 ng/mL of rmM-CSF to generate bone marrow-derived macrophages (BMMs).

    Article Title: Testing Models of the APC Tumor Suppressor/β-Catenin Interaction Reshapes Our View of the Destruction Complex in Wnt Signaling
    Article Snippet: .. SW480 cells were cultured at 37° and 5% CO2 in DMEM-H supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1× Pen/Strep/Glutamine (GIBCO, Grand Island, NY). .. For transient transfections, SW480 cells were plated at a density of 2.5 × 105 cells per well in six-well plates and grown overnight.

    Imaging:

    Article Title: In vivo fluorescence correlation spectroscopy analyses of FMBP‐1, a silkworm transcription factor
    Article Snippet: LSM imaging and FCS measurement Both LSM imaging and FCS measurements were performed using an LSM510 inverted confocal laser scanning microscope (Carl Zeiss, Jena, Germany), which comprised a CW Ar+ laser, a water immersion objective (C‐Apochromat, 40×, 1.2 NA; Carl Zeiss), and a ConfoCor 3 (Carl Zeiss). .. For LSM imaging of PSG cells, the cells were stained with 25 μg·mL−1 Hoechst 33342 (Invitrogen) to visualize the nuclei. ..

    Staining:

    Article Title: In vivo fluorescence correlation spectroscopy analyses of FMBP‐1, a silkworm transcription factor
    Article Snippet: LSM imaging and FCS measurement Both LSM imaging and FCS measurements were performed using an LSM510 inverted confocal laser scanning microscope (Carl Zeiss, Jena, Germany), which comprised a CW Ar+ laser, a water immersion objective (C‐Apochromat, 40×, 1.2 NA; Carl Zeiss), and a ConfoCor 3 (Carl Zeiss). .. For LSM imaging of PSG cells, the cells were stained with 25 μg·mL−1 Hoechst 33342 (Invitrogen) to visualize the nuclei. ..

    Article Title: MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1
    Article Snippet: In the present study, clone formation assay, flow cytometry, transwell chamber and other experimental methods were used to investigate the effect of overexpression of miR-486-3p on the proliferation, apoptosis and invasion ability of RB, in order to provide new ideas for clinical treatment of RB. .. ReagentsRPMI-1640 medium (31870082; Gibco, U.S.A.); 10% fetal bovine serum (FBS, 16140071; Gibco, U.S.A.); 100 U/ml penicillin (10378016; Gibco, U.S.A.); 100 μg/ml streptomycin (10378016; Gibco, U.S.A.); Lipofectamine 2000 Transfection Reagent (11668030; Invitrogen, U.S.A.); Wright’s–Giemsa’s staining solution (G5225; GBCBIO technologies Inc., China); paraformaldehyde (P0099-500ml; Beyotime, China); Crystal Violet (C0121; Beyotime, China); TRIzol reagent (15596026; Invitrogen, U.S.A.); SYBR miRNA detection assays (Takara, China); RIPA buffer (P0013K; Beyotime, China); PVDF membrane (Merck, Germany); Cell counting kit-8 (CCK-8, C0037; Beyotime, China); Annexin V-FITC Apoptosis Detection kit (APOAF; Sigma–Aldrich, U.S.A.); PrimeScript RT Master Mix kit (RR036A; Takara, China); BCA protein assay kit (23225; Pierce, Germany). ..

    Isolation:

    Article Title: Type I IL-4 Receptors Selectively Activate IRS-2 to Induce Target Gene Expression in Macrophages
    Article Snippet: Murine bone marrow-derived macrophages were generated from either WT C57BL/6 (Taconic Laboratories, Germantown, NY) or γC−/− (breeding pairs obtained from Taconic and bred in-house). .. Briefly, bone marrow was isolated from femurs and tibias of four- to six-week-old female mice.. To deplete adherent stromal cells, the harvested bone marrow was cultured overnight in α-10 medium [α-Minimal Essential Medium supplemented with penicillin, streptomycin, and glutamine (BioWhittaker) and 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Frederick, MD)] with 20 ng/mL of recombinant murine macrophage colony-stimulating factor [rmM-CSF, R & D Systems, Minneapolis, MN)]. .. Nonadherent cells were collected, red blood cell lysis was performed, and nonadherent mononuclear cellswere plated and cultured for ten days in the presence of 20 ng/mL of rmM-CSF to generate bone marrow-derived macrophages (BMMs).

    Mouse Assay:

    Article Title: Type I IL-4 Receptors Selectively Activate IRS-2 to Induce Target Gene Expression in Macrophages
    Article Snippet: Murine bone marrow-derived macrophages were generated from either WT C57BL/6 (Taconic Laboratories, Germantown, NY) or γC−/− (breeding pairs obtained from Taconic and bred in-house). .. Briefly, bone marrow was isolated from femurs and tibias of four- to six-week-old female mice.. To deplete adherent stromal cells, the harvested bone marrow was cultured overnight in α-10 medium [α-Minimal Essential Medium supplemented with penicillin, streptomycin, and glutamine (BioWhittaker) and 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Frederick, MD)] with 20 ng/mL of recombinant murine macrophage colony-stimulating factor [rmM-CSF, R & D Systems, Minneapolis, MN)]. .. Nonadherent cells were collected, red blood cell lysis was performed, and nonadherent mononuclear cellswere plated and cultured for ten days in the presence of 20 ng/mL of rmM-CSF to generate bone marrow-derived macrophages (BMMs).

    Recombinant:

    Article Title: Type I IL-4 Receptors Selectively Activate IRS-2 to Induce Target Gene Expression in Macrophages
    Article Snippet: Murine bone marrow-derived macrophages were generated from either WT C57BL/6 (Taconic Laboratories, Germantown, NY) or γC−/− (breeding pairs obtained from Taconic and bred in-house). .. Briefly, bone marrow was isolated from femurs and tibias of four- to six-week-old female mice.. To deplete adherent stromal cells, the harvested bone marrow was cultured overnight in α-10 medium [α-Minimal Essential Medium supplemented with penicillin, streptomycin, and glutamine (BioWhittaker) and 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Frederick, MD)] with 20 ng/mL of recombinant murine macrophage colony-stimulating factor [rmM-CSF, R & D Systems, Minneapolis, MN)]. .. Nonadherent cells were collected, red blood cell lysis was performed, and nonadherent mononuclear cellswere plated and cultured for ten days in the presence of 20 ng/mL of rmM-CSF to generate bone marrow-derived macrophages (BMMs).

    other:

    Article Title: Ex vivo expansion of cord blood-derived endothelial cells using a novel xeno-free culture media
    Article Snippet: Endothelial cell media (ECM) α-Minimum essential medium (α-MEM; Mediatech, Inc., VA, USA) supplemented with 20% fetal bovine serum (FBS; Akron Biotech, FL, USA), 1% penicillin–streptomycinæ Glutamine (GPS, Gibco), 20 ng/ml bFGF, 10 ng/ml VEGF and 10 ng/ml EGF.

    Transfection:

    Article Title: MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1
    Article Snippet: In the present study, clone formation assay, flow cytometry, transwell chamber and other experimental methods were used to investigate the effect of overexpression of miR-486-3p on the proliferation, apoptosis and invasion ability of RB, in order to provide new ideas for clinical treatment of RB. .. ReagentsRPMI-1640 medium (31870082; Gibco, U.S.A.); 10% fetal bovine serum (FBS, 16140071; Gibco, U.S.A.); 100 U/ml penicillin (10378016; Gibco, U.S.A.); 100 μg/ml streptomycin (10378016; Gibco, U.S.A.); Lipofectamine 2000 Transfection Reagent (11668030; Invitrogen, U.S.A.); Wright’s–Giemsa’s staining solution (G5225; GBCBIO technologies Inc., China); paraformaldehyde (P0099-500ml; Beyotime, China); Crystal Violet (C0121; Beyotime, China); TRIzol reagent (15596026; Invitrogen, U.S.A.); SYBR miRNA detection assays (Takara, China); RIPA buffer (P0013K; Beyotime, China); PVDF membrane (Merck, Germany); Cell counting kit-8 (CCK-8, C0037; Beyotime, China); Annexin V-FITC Apoptosis Detection kit (APOAF; Sigma–Aldrich, U.S.A.); PrimeScript RT Master Mix kit (RR036A; Takara, China); BCA protein assay kit (23225; Pierce, Germany). ..

    CCK-8 Assay:

    Article Title: MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1
    Article Snippet: In the present study, clone formation assay, flow cytometry, transwell chamber and other experimental methods were used to investigate the effect of overexpression of miR-486-3p on the proliferation, apoptosis and invasion ability of RB, in order to provide new ideas for clinical treatment of RB. .. ReagentsRPMI-1640 medium (31870082; Gibco, U.S.A.); 10% fetal bovine serum (FBS, 16140071; Gibco, U.S.A.); 100 U/ml penicillin (10378016; Gibco, U.S.A.); 100 μg/ml streptomycin (10378016; Gibco, U.S.A.); Lipofectamine 2000 Transfection Reagent (11668030; Invitrogen, U.S.A.); Wright’s–Giemsa’s staining solution (G5225; GBCBIO technologies Inc., China); paraformaldehyde (P0099-500ml; Beyotime, China); Crystal Violet (C0121; Beyotime, China); TRIzol reagent (15596026; Invitrogen, U.S.A.); SYBR miRNA detection assays (Takara, China); RIPA buffer (P0013K; Beyotime, China); PVDF membrane (Merck, Germany); Cell counting kit-8 (CCK-8, C0037; Beyotime, China); Annexin V-FITC Apoptosis Detection kit (APOAF; Sigma–Aldrich, U.S.A.); PrimeScript RT Master Mix kit (RR036A; Takara, China); BCA protein assay kit (23225; Pierce, Germany). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: MiR-486-3p inhibits the proliferation, migration and invasion of retinoblastoma cells by targeting ECM1
    Article Snippet: In the present study, clone formation assay, flow cytometry, transwell chamber and other experimental methods were used to investigate the effect of overexpression of miR-486-3p on the proliferation, apoptosis and invasion ability of RB, in order to provide new ideas for clinical treatment of RB. .. ReagentsRPMI-1640 medium (31870082; Gibco, U.S.A.); 10% fetal bovine serum (FBS, 16140071; Gibco, U.S.A.); 100 U/ml penicillin (10378016; Gibco, U.S.A.); 100 μg/ml streptomycin (10378016; Gibco, U.S.A.); Lipofectamine 2000 Transfection Reagent (11668030; Invitrogen, U.S.A.); Wright’s–Giemsa’s staining solution (G5225; GBCBIO technologies Inc., China); paraformaldehyde (P0099-500ml; Beyotime, China); Crystal Violet (C0121; Beyotime, China); TRIzol reagent (15596026; Invitrogen, U.S.A.); SYBR miRNA detection assays (Takara, China); RIPA buffer (P0013K; Beyotime, China); PVDF membrane (Merck, Germany); Cell counting kit-8 (CCK-8, C0037; Beyotime, China); Annexin V-FITC Apoptosis Detection kit (APOAF; Sigma–Aldrich, U.S.A.); PrimeScript RT Master Mix kit (RR036A; Takara, China); BCA protein assay kit (23225; Pierce, Germany). ..

    Laser Capture Microdissection:

    Article Title: TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages
    Article Snippet: .. The cells were then resuspended at 5 × 106 cells/mL in BMDM media: RPMI1640 (Gibco) supplemented with 20% inactivated FBS, 30% L929-conditioned media (LCM), penicillin/streptomycin/L-glutamine (1 unit/mL, 1 μ g/mL, 2 mM; Gibco), β-mercaptoethanol (0.05 M; Sigma), and 10 mL plated/10 cm sterile nontissue culture-treated Petri dish (Corning). ..

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  • 97
    Thermo Fisher synthetic mixture b27
    Absence of apoptotic cell death after N-CAM treatment. Hippocampal cells were plated in <t>NB/B27</t> with 20 ng/ml bFGF for 48 hr and treated for 2 additional days with 10 μg/ml N-CAM. Apoptosis was assessed using the TUNEL method as described in Materials and Methods. Green fluorescent nuclei correspond to apoptotic cells in which the terminal transferase has incorporated fluorescent dUTP ( Bodipy-dUTP ) into fragmented DNA. All the cells were counterstained with the nuclear stain DAPI shown in blue . A minimum of 10 independent fields was used to assess the percentage of apoptotic cells (see Results).
    Synthetic Mixture B27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synthetic mixture b27/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    synthetic mixture b27 - by Bioz Stars, 2021-03
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    99
    Thermo Fisher 100 mm petri dish
    Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, <t>100</t> μm.
    100 Mm Petri Dish, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 mm petri dish/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    100 mm petri dish - by Bioz Stars, 2021-03
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    99
    Thermo Fisher msc media
    (A) A representative image of the formation of a multi-cellular sprout in the EC: <t>MSC</t> co-culture condition in 50/50% MSC medium. (B) A representative image of the formation of a multi-cellular sprout in the EC: CF co-culture condition in 50/50% CF medium. Scale bar = 100um. Blue = DAPI, Green = CD31 and Red = vWF. (C) Quantification of the number of nuclei per sprout for EC: CF and EC: MSC co-cultures in fibrin gels (* p=0.007, + p=0.002). (D) Quantification of the number of nuclei per sprout for EC-only culture in fibrin gels in the various media formulations.
    Msc Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msc media/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    msc media - by Bioz Stars, 2021-03
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    Image Search Results


    Absence of apoptotic cell death after N-CAM treatment. Hippocampal cells were plated in NB/B27 with 20 ng/ml bFGF for 48 hr and treated for 2 additional days with 10 μg/ml N-CAM. Apoptosis was assessed using the TUNEL method as described in Materials and Methods. Green fluorescent nuclei correspond to apoptotic cells in which the terminal transferase has incorporated fluorescent dUTP ( Bodipy-dUTP ) into fragmented DNA. All the cells were counterstained with the nuclear stain DAPI shown in blue . A minimum of 10 independent fields was used to assess the percentage of apoptotic cells (see Results).

    Journal: The Journal of Neuroscience

    Article Title: N-CAM Binding Inhibits the Proliferation of Hippocampal Progenitor Cells and Promotes Their Differentiation to a Neuronal Phenotype

    doi: 10.1523/JNEUROSCI.20-10-03631.2000

    Figure Lengend Snippet: Absence of apoptotic cell death after N-CAM treatment. Hippocampal cells were plated in NB/B27 with 20 ng/ml bFGF for 48 hr and treated for 2 additional days with 10 μg/ml N-CAM. Apoptosis was assessed using the TUNEL method as described in Materials and Methods. Green fluorescent nuclei correspond to apoptotic cells in which the terminal transferase has incorporated fluorescent dUTP ( Bodipy-dUTP ) into fragmented DNA. All the cells were counterstained with the nuclear stain DAPI shown in blue . A minimum of 10 independent fields was used to assess the percentage of apoptotic cells (see Results).

    Article Snippet: The resulting dissociated cells were centrifuged and resuspended in Neurobasal medium supplemented with penicillin, streptomycin, glutamine, and the synthetic mixture B27 (Life Technologies) (NB/B27), in the presence of 20 ng/ml basic FGF (bFGF; Sigma).

    Techniques: Chick Chorioallantoic Membrane Assay, TUNEL Assay, Staining

    Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, 100 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

    doi: 10.3390/ijms18112348

    Figure Lengend Snippet: Cancer microtissues ( a – f ); monocultured microtissue and fibroblast-layered microtissue (1 × 10 4 cell/mL) on day 7. Red, MMP2 ( a ) and MMP9 ( d ); green, F-actin ( b , e ). MMP2 ( g ) and MMP9 ( h ) expression in the mono-cultured and fibroblast-layered microtissues was quantified using ELISA. All experimental samples were n > 3. Unmarked Scale bars, 100 μm.

    Article Snippet: Cell and Bioink Preparation Fibroblasts (CCD-1112SK, ATCC, Manassas, VA, USA) and HPV18-positive cervical cancer cells (HeLa; KCLB, Seoul, Korea) were cultured with a medium containing 10% FBS and 1% Pen Strep in a 100-mm Petri dish (Nunclon Delta Surface, Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    (A) A representative image of the formation of a multi-cellular sprout in the EC: MSC co-culture condition in 50/50% MSC medium. (B) A representative image of the formation of a multi-cellular sprout in the EC: CF co-culture condition in 50/50% CF medium. Scale bar = 100um. Blue = DAPI, Green = CD31 and Red = vWF. (C) Quantification of the number of nuclei per sprout for EC: CF and EC: MSC co-cultures in fibrin gels (* p=0.007, + p=0.002). (D) Quantification of the number of nuclei per sprout for EC-only culture in fibrin gels in the various media formulations.

    Journal: Annals of biomedical engineering

    Article Title: Cardiac Fibroblasts Support Endothelial Cell Proliferation and Sprout Formation but not the Development of Multicellular Sprouts in a Fibrin Gel Co-Culture Model

    doi: 10.1007/s10439-014-0971-2

    Figure Lengend Snippet: (A) A representative image of the formation of a multi-cellular sprout in the EC: MSC co-culture condition in 50/50% MSC medium. (B) A representative image of the formation of a multi-cellular sprout in the EC: CF co-culture condition in 50/50% CF medium. Scale bar = 100um. Blue = DAPI, Green = CD31 and Red = vWF. (C) Quantification of the number of nuclei per sprout for EC: CF and EC: MSC co-cultures in fibrin gels (* p=0.007, + p=0.002). (D) Quantification of the number of nuclei per sprout for EC-only culture in fibrin gels in the various media formulations.

    Article Snippet: Rat MSCs were purchased from Texas A & M University at passage 6 and cultured in MSC media (alpha-MEM, 20% FBS, 1% penicillin/streptomycin and supplemented daily with 2% L-Glutamine; all from Invitrogen) and used in experiments from passage 8–12.

    Techniques: Co-Culture Assay