penicillin g  (Thermo Fisher)


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    Name:
    Penicillin G M I C Evaluator Strips M I C E
    Description:
    Effortlessly establish accurate Minimum Inhibitory Concentration MIC values manually using Thermo Scientific Oxoid Penicillin G M I C Evaluator Strips M I C E
    Catalog Number:
    ma0100d
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    Category:
    Kits and Assays
    Applications:
    Clinical|Clinical Microbiology
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    Structured Review

    Thermo Fisher penicillin g
    Effortlessly establish accurate Minimum Inhibitory Concentration MIC values manually using Thermo Scientific Oxoid Penicillin G M I C Evaluator Strips M I C E
    https://www.bioz.com/result/penicillin g/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin g - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Modification:

    Article Title: Lipopolysaccharide Lowers Cholesteryl Ester Transfer Protein by Activating F4/80+Clec4f+Vsig4+Ly6C− Kupffer Cell Subsets
    Article Snippet: Monocytes were then purified using human CD14 magnetic beads and MACS® cell separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Monocytes were plated in 24‐well tissue culture plates at a density of 1×106 cells/mL (500 μL per well) and differentiated to macrophages for 6 days in Iscove's Modified Dulbecco's Medium (Sigma‐Aldrich) supplemented with 2 mmol/L l ‐glutamine, penicillin (100 U/mL), streptomycin (100 μg/mL) and 10% fetal calf serum (All Gibco, Waltham, MA) in the presence of 50 ng/mL macrophage colony stimulating factor (MCSF) (Miltenyi Biotec, Bergisch Gladbach, Germany). .. On day 3, the medium was removed and substituted by fresh Iscove's Modified Dulbecco's Medium with 10% fetal calf serum and 50 ng/mL MCSF.

    Article Title: Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome
    Article Snippet: .. SHED were isolated from dental pulp tissues as described previously , and grown in a SHED culture medium consisting of Minimum Essential Medium Eagle Alpha Modification (Sigma-Aldrich, MO, USA) with 15% fetal bovine serum (Sigma-Aldrich), 100 µM L-ascorbic 2-phosphate (Wako Pure Chemical Industries, Osaka, Japan), 2 mM L -glutamine (Life Technologies, NY, USA), 100 U/mL penicillin (Life Technologies), 100 µg/mL streptomycin (Life Technologies) and 25 µg/mL Fungizone (Life Technologies) at 37 °C in 5% CO2 . ..

    Cell Culture:

    Article Title: Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells
    Article Snippet: Peripheral Blood Mononuclear Cells (PBMCs) were isolated by density gradient centrifugation using Lymphocyte separation medium (EurobioTM, Montpellier, France). .. Cells were washed three times with PBS, subsequently stimulated with 1 mg/ml phytohemagglutinin-P (PHA) (SigmaTM, Saint Louis, Missouri, USA) and cultured in DMEM supplemented with 1% (v/v) sodium pyruvate, non-essential aminoacids, vitamins, L-arginin, L-asparragin, folic acid, 10 mM Hepes, 50 mM 2-mercaptoethanol, 100 mg/ml streptomycin, 100 U/ml penicillin (Life Technologies, Carlsbad, CA, USA) and 10% heat-inactivated FBS (Gibco) at 37°C, in a 10% CO2 atmosphere. ..

    Isolation:

    Article Title: Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome
    Article Snippet: .. SHED were isolated from dental pulp tissues as described previously , and grown in a SHED culture medium consisting of Minimum Essential Medium Eagle Alpha Modification (Sigma-Aldrich, MO, USA) with 15% fetal bovine serum (Sigma-Aldrich), 100 µM L-ascorbic 2-phosphate (Wako Pure Chemical Industries, Osaka, Japan), 2 mM L -glutamine (Life Technologies, NY, USA), 100 U/mL penicillin (Life Technologies), 100 µg/mL streptomycin (Life Technologies) and 25 µg/mL Fungizone (Life Technologies) at 37 °C in 5% CO2 . ..

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    • penicillin g  (Thermo Fisher)
    97
    Thermo Fisher penicillin g
    Genomic insertion of the PcCluster-transformed A. fumigatus into a <t>penicillin</t> G producer. (a) To facilitate genomic integration of the PcCluster at the fcyB locus, the plasmid p fcyB -PcCluster comprising the respective DOI (17 kb) as well as fcyB 5′ and 3′ NTRs was generated. Linearization of this plasmid with PmeI allows homologous recombination-based replacement of fcyB coding sequence with DNA containing the PcCluster. (b) Expression of functional pcbAB , pcbC and penDE was monitored in three independent transformants using Northern blot analysis ( gpdA was used as reference). (c) LC-MS/MS-extracted ion chromatograms of penicillin G (peak at 45 min) and its degradation product penillic acid (peak at 27 min) in the culture supernatant of fcyB PENG strain after shaking incubation for 48 h at 25°C. wt served as a negative control.
    Penicillin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin g/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin g - by Bioz Stars, 2021-03
    97/100 stars
    		  Buy from Supplier
    tetracycline m i c evaluator strips  (Thermo Fisher)
    93
    Thermo Fisher tetracycline m i c evaluator strips
    Vancomycin fluorescence (Van-FL) staining and penicillin susceptibility of the WT, ponA Y489C mutant, and Δ ponA mutant strains. (A) Microscope images of Van-FL-stained cells under fluorescence. Bars represent 5 μm. (B) Fluorescence intensities of Van-FL stained cells. When the OD 600 of the culture reached 1.0, Van-FL was added to the culture with a final concentration of 100 µg/ml. The mixture was incubated for another 30 min at 30°C. After washing 3 times with PBS buffer, the Van-FL-stained cells were used for fluorescence microscope observation (λ excitation = 480 nm, λ emission = 527 nm) and fluorescence intensity determination by using a fluorimeter (λ excitation = 490 nm, λ emission = 520 nm). Error bars indicate standard deviations from the results from three parallel experiments. Asterisks indicate significant changes in fluorescence intensity based on a comparison between the mutants and the wild-type strain. ***, P ≤ 0.001 (Student’s two-tailed t test). (C) MICs of penicillin and tetracycline for different strains. Three parallel experiments were conducted, and the same MIC was obtained. (D) Determination of penicillin MIC using <t>M.I.C.Evaluator</t> strips (256 to 0.015 μg/ml). One of the three parallel experiments is shown here.
    Tetracycline M I C Evaluator Strips, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tetracycline m i c evaluator strips/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tetracycline m i c evaluator strips - by Bioz Stars, 2021-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Genomic insertion of the PcCluster-transformed A. fumigatus into a penicillin G producer. (a) To facilitate genomic integration of the PcCluster at the fcyB locus, the plasmid p fcyB -PcCluster comprising the respective DOI (17 kb) as well as fcyB 5′ and 3′ NTRs was generated. Linearization of this plasmid with PmeI allows homologous recombination-based replacement of fcyB coding sequence with DNA containing the PcCluster. (b) Expression of functional pcbAB , pcbC and penDE was monitored in three independent transformants using Northern blot analysis ( gpdA was used as reference). (c) LC-MS/MS-extracted ion chromatograms of penicillin G (peak at 45 min) and its degradation product penillic acid (peak at 27 min) in the culture supernatant of fcyB PENG strain after shaking incubation for 48 h at 25°C. wt served as a negative control.

    Journal: mBio

    Article Title: Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

    doi: 10.1128/mBio.00230-20

    Figure Lengend Snippet: Genomic insertion of the PcCluster-transformed A. fumigatus into a penicillin G producer. (a) To facilitate genomic integration of the PcCluster at the fcyB locus, the plasmid p fcyB -PcCluster comprising the respective DOI (17 kb) as well as fcyB 5′ and 3′ NTRs was generated. Linearization of this plasmid with PmeI allows homologous recombination-based replacement of fcyB coding sequence with DNA containing the PcCluster. (b) Expression of functional pcbAB , pcbC and penDE was monitored in three independent transformants using Northern blot analysis ( gpdA was used as reference). (c) LC-MS/MS-extracted ion chromatograms of penicillin G (peak at 45 min) and its degradation product penillic acid (peak at 27 min) in the culture supernatant of fcyB PENG strain after shaking incubation for 48 h at 25°C. wt served as a negative control.

    Article Snippet: Nano-scale liquid chromatography-mass spectrometry (nanoLC-MS)-based detection of penicillin G was conducted using an UltiMate 3000 nano-scale high-performance liquid chromatography (HPLC) system coupled to a Q Exactive HF mass spectrometer (Thermo Scientific, Bremen, Germany).

    Techniques: Transformation Assay, Plasmid Preparation, Generated, Homologous Recombination, Sequencing, Expressing, Functional Assay, Northern Blot, Liquid Chromatography with Mass Spectroscopy, Incubation, Negative Control

    Genomic insertion of the PcCluster transformed A. fumigatus into a penicillin G producer. (a) To facilitate genomic integration of the PcCluster at the fcyB locus, the plasmid p fcyB -PcCluster comprising the respective DOI (17 kb) as well as fcyB 5’ and 3’ NTRs was generated. Linearization of this plasmid with PmeI allows homologous recombination-based replacement of fcyB coding sequence with DNA containing the PcCluster. (b) Expression of functional pcbAB, pcbC and penDE was monitored in three independent transformants using Northern blot analysis (gpdA was used as reference). (c) LC-MS/MS extracted ion chromatograms of penicillin G (peak at 45 min) and its degradation product penillic acid (peak at 27 min) in the culture supernatant of fcyB PENG after shaking incubation for 48 h at 25 °C. wt served as negative control.

    Journal: bioRxiv

    Article Title: Multiplex genetic engineering exploiting pyrimidine salvage pathway-based self-encoded selectable markers

    doi: 10.1101/2020.01.16.908764

    Figure Lengend Snippet: Genomic insertion of the PcCluster transformed A. fumigatus into a penicillin G producer. (a) To facilitate genomic integration of the PcCluster at the fcyB locus, the plasmid p fcyB -PcCluster comprising the respective DOI (17 kb) as well as fcyB 5’ and 3’ NTRs was generated. Linearization of this plasmid with PmeI allows homologous recombination-based replacement of fcyB coding sequence with DNA containing the PcCluster. (b) Expression of functional pcbAB, pcbC and penDE was monitored in three independent transformants using Northern blot analysis (gpdA was used as reference). (c) LC-MS/MS extracted ion chromatograms of penicillin G (peak at 45 min) and its degradation product penillic acid (peak at 27 min) in the culture supernatant of fcyB PENG after shaking incubation for 48 h at 25 °C. wt served as negative control.

    Article Snippet: NanoLC-MS-based detection of penicillin G was carried out using an UltiMate 3000 nano-HPLC system coupled to a Q Exactive HF mass spectrometer (Thermo Scientific, Bremen, Germany).

    Techniques: Transformation Assay, Plasmid Preparation, Generated, Homologous Recombination, Sequencing, Expressing, Functional Assay, Northern Blot, Liquid Chromatography with Mass Spectroscopy, Incubation, Negative Control

    Reduced susceptibility rate of N. meningitidis isolates to penicillin G during the period from 2010 to 2019 ( n =183).

    Journal: Access Microbiology

    Article Title: Epidemiological profile of Neisseria meningitidis in Casablanca, Morocco: 2010–2019

    doi: 10.1099/acmi.0.000157

    Figure Lengend Snippet: Reduced susceptibility rate of N. meningitidis isolates to penicillin G during the period from 2010 to 2019 ( n =183).

    Article Snippet: The MICs of penicillin G, ampicillin and ceftriaxone were determined by E-test (Oxoid Thermofisher, UK) on Mueller–Hinton agar in addition to 5 % of sheep blood (MHS) (BioMérieux, Marcy l’Etoile.

    Techniques:

    Vancomycin fluorescence (Van-FL) staining and penicillin susceptibility of the WT, ponA Y489C mutant, and Δ ponA mutant strains. (A) Microscope images of Van-FL-stained cells under fluorescence. Bars represent 5 μm. (B) Fluorescence intensities of Van-FL stained cells. When the OD 600 of the culture reached 1.0, Van-FL was added to the culture with a final concentration of 100 µg/ml. The mixture was incubated for another 30 min at 30°C. After washing 3 times with PBS buffer, the Van-FL-stained cells were used for fluorescence microscope observation (λ excitation = 480 nm, λ emission = 527 nm) and fluorescence intensity determination by using a fluorimeter (λ excitation = 490 nm, λ emission = 520 nm). Error bars indicate standard deviations from the results from three parallel experiments. Asterisks indicate significant changes in fluorescence intensity based on a comparison between the mutants and the wild-type strain. ***, P ≤ 0.001 (Student’s two-tailed t test). (C) MICs of penicillin and tetracycline for different strains. Three parallel experiments were conducted, and the same MIC was obtained. (D) Determination of penicillin MIC using M.I.C.Evaluator strips (256 to 0.015 μg/ml). One of the three parallel experiments is shown here.

    Journal: Applied and Environmental Microbiology

    Article Title: Mutations in Peptidoglycan Synthesis Gene ponA Improve Electrotransformation Efficiency of Corynebacterium glutamicum ATCC 13869

    doi: 10.1128/AEM.02225-18

    Figure Lengend Snippet: Vancomycin fluorescence (Van-FL) staining and penicillin susceptibility of the WT, ponA Y489C mutant, and Δ ponA mutant strains. (A) Microscope images of Van-FL-stained cells under fluorescence. Bars represent 5 μm. (B) Fluorescence intensities of Van-FL stained cells. When the OD 600 of the culture reached 1.0, Van-FL was added to the culture with a final concentration of 100 µg/ml. The mixture was incubated for another 30 min at 30°C. After washing 3 times with PBS buffer, the Van-FL-stained cells were used for fluorescence microscope observation (λ excitation = 480 nm, λ emission = 527 nm) and fluorescence intensity determination by using a fluorimeter (λ excitation = 490 nm, λ emission = 520 nm). Error bars indicate standard deviations from the results from three parallel experiments. Asterisks indicate significant changes in fluorescence intensity based on a comparison between the mutants and the wild-type strain. ***, P ≤ 0.001 (Student’s two-tailed t test). (C) MICs of penicillin and tetracycline for different strains. Three parallel experiments were conducted, and the same MIC was obtained. (D) Determination of penicillin MIC using M.I.C.Evaluator strips (256 to 0.015 μg/ml). One of the three parallel experiments is shown here.

    Article Snippet: M.I.C.Evaluator strips (Oxoid; penicillin, 256 to 0.015 μg/ml, product no. MA0101D; tetracycline, 256 to 0.015 μg/ml, product no. MA0105D) were used for determining the MICs of penicillin and tetracycline.

    Techniques: Fluorescence, Staining, Mutagenesis, Microscopy, Concentration Assay, Incubation, Two Tailed Test