penicillin g  (millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Penicillin V
    Description:
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    Catalog Number:
    1504489
    Price:
    None
    Buy from Supplier


    Structured Review

    millipore penicillin g
    Penicillin V
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    https://www.bioz.com/result/penicillin g/product/millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin g - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Development of a Novel Multipenicillin Assay and Assessment of the Impact of Analyte Degradation: Lessons for Scavenged Sampling in Antimicrobial Pharmacokinetic Study Design"

    Article Title: Development of a Novel Multipenicillin Assay and Assessment of the Impact of Analyte Degradation: Lessons for Scavenged Sampling in Antimicrobial Pharmacokinetic Study Design

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01540-17

    Chemical structures of amoxicillin (a), penicillin G (b), piperacillin (c), flucloxacillin (d), and ampicillin (e).
    Figure Legend Snippet: Chemical structures of amoxicillin (a), penicillin G (b), piperacillin (c), flucloxacillin (d), and ampicillin (e).

    Techniques Used:

    2) Product Images from "Towards a minimally invasive device for beta-lactam monitoring in humans"

    Article Title: Towards a minimally invasive device for beta-lactam monitoring in humans

    Journal: Electrochemistry communications

    doi: 10.1016/j.elecom.2017.07.011

    Calibration of microneedle biosensors 2a. Calibration curve comparison for microneedle runs with penicillin-G fitted with the Hill equation 2b. Log(concentration) plots of data from penicillin-G calibration 2c. Comparison of results for platinum disc electrode based beta-lactam sensor calibrated against penicillin-G, amoxicillin, and ceftriaxone.
    Figure Legend Snippet: Calibration of microneedle biosensors 2a. Calibration curve comparison for microneedle runs with penicillin-G fitted with the Hill equation 2b. Log(concentration) plots of data from penicillin-G calibration 2c. Comparison of results for platinum disc electrode based beta-lactam sensor calibrated against penicillin-G, amoxicillin, and ceftriaxone.

    Techniques Used:

    3) Product Images from "Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar Effects"

    Article Title: Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar Effects

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00479-18

    Construction of a markerless Chlamydia tmeA mutant. (A) Schematic representation of the strategy used to create a markerless tmeA mutant. Each intermediate is depicted with fluorescent qualities (green, GFP + ; red, mCherry + ; gray, no fluorescence) and antibiotic sensitivities. (B) McCoy cell cultures were infected with equal IFUs of WT, L2 tmeA , or L2 Rif tmeA-lx C. trachomatis and serially passaged every 24 h in medium containing PenG. At each passage, DNA was harvested for quantitative real-time PCR to determine genome equivalents based on chlamydial 16S rRNA. (C) qPCR-based comparison of endogenous pL2 (plasmid) copy number in WT or tmeA-lx mutant chlamydiae relative to chlamydial 16S rRNA. DNA was harvested from infected McCoy cells at 24 hpi. PenG r , penicillin G resistant; PenG s , penicillin susceptible; Rif r , rifampin resistant; Spec s , spectinomycin susceptible; Spec r , spectinomycin resistant.
    Figure Legend Snippet: Construction of a markerless Chlamydia tmeA mutant. (A) Schematic representation of the strategy used to create a markerless tmeA mutant. Each intermediate is depicted with fluorescent qualities (green, GFP + ; red, mCherry + ; gray, no fluorescence) and antibiotic sensitivities. (B) McCoy cell cultures were infected with equal IFUs of WT, L2 tmeA , or L2 Rif tmeA-lx C. trachomatis and serially passaged every 24 h in medium containing PenG. At each passage, DNA was harvested for quantitative real-time PCR to determine genome equivalents based on chlamydial 16S rRNA. (C) qPCR-based comparison of endogenous pL2 (plasmid) copy number in WT or tmeA-lx mutant chlamydiae relative to chlamydial 16S rRNA. DNA was harvested from infected McCoy cells at 24 hpi. PenG r , penicillin G resistant; PenG s , penicillin susceptible; Rif r , rifampin resistant; Spec s , spectinomycin susceptible; Spec r , spectinomycin resistant.

    Techniques Used: Mutagenesis, Fluorescence, Infection, Real-time Polymerase Chain Reaction, Plasmid Preparation

    Related Articles

    Concentration Assay:

    Article Title: A 2-Pyridone-Amide Inhibitor Targets the Glucose Metabolism Pathway of Chlamydia trachomatis
    Article Snippet: .. Penicillin-G was dissolved in water to reach a concentration of 80,000 U/ml and filter sterilized (Millipore) (0.22-µm-pore-size filter). ..

    Article Title: Penicillin G-Induced Chlamydial Stress Response in a Porcine Strain of Chlamydia pecorum
    Article Snippet: SPG medium consisted of 218 mM sucrose (Sigma-Aldrich), 3.76 mM KH2 PO4 (Sigma-Aldrich), 7.1 mM K2 HPO4 (Merck Eurolab AG, Dietlikon, Switzerland), and 5 mM GlutaMAX-100 (GIBCO). .. Penicillin Reagent Penicillin G sodium salt (Sigma-Aldrich) was dissolved in sterile deionized water to a stock concentration of 20,000 units (U)/mL, filter-sterilized, and stored at −20°C. .. Aliquots of this penicillin G stock were thawed and further diluted in sterile water to a working concentration of 100 U/mL immediately before use.

    Cell Culture:

    Article Title: Invasion of Porcine Brain Microvascular Endothelial Cells by Streptococcus suis Serotype 2
    Article Snippet: The plates were centrifuged at 800 × g for 10 min to bring the bacteria to the surface of the monolayer and incubated for different times (see Results) at 37°C with 5% CO2 to allow cell invasion by the bacteria. .. The monolayers were then washed twice with PBS, and 1 ml of cell culture medium containing 100 μg of gentamicin per ml and 5 μg of penicillin G (Sigma) per ml was added to each well. .. The plates were then incubated for 1 h at 37°C with 5% CO2 to kill extracellular and surface-adherent bacteria.

    High Performance Liquid Chromatography:

    Article Title: Terminalia chebula Retz. Fruit Extracts Inhibit Bacterial Triggers of Some Autoimmune Diseases and Potentiate the Activity of Tetracycline
    Article Snippet: .. Penicillin-G (potency of 1440–1680 µg/mg), chloramphenicol (≥ 98% purity by HPLC, erythromycin (potency ≥ 850 µg/mg), gentamicin (potency of 600 µg/mg), and tetracycline (≥ 95% purity by HPLC) were purchased from Sigma-Aldrich, Australia and were used as controls for the microplate liquid dilution assay. ..

    Dilution Assay:

    Article Title: Terminalia chebula Retz. Fruit Extracts Inhibit Bacterial Triggers of Some Autoimmune Diseases and Potentiate the Activity of Tetracycline
    Article Snippet: .. Penicillin-G (potency of 1440–1680 µg/mg), chloramphenicol (≥ 98% purity by HPLC, erythromycin (potency ≥ 850 µg/mg), gentamicin (potency of 600 µg/mg), and tetracycline (≥ 95% purity by HPLC) were purchased from Sigma-Aldrich, Australia and were used as controls for the microplate liquid dilution assay. ..

    Chromatography:

    Article Title: A protein of the metallo-hydrolase/oxidoreductase superfamily with both beta-lactamase and ribonuclease activity is linked with translation in giant viruses
    Article Snippet: In order to determine kinetic parameters, initial velocities were calculated by Gen5.1 software (BioTek, Winooski, VT, USA) and obtained mean values were fitted using the Michaelis–Menten equation on Prism 6 (GraphPad Software, San Diego, CA, USA). .. Beta-lactam antibiotic degradation monitoring by liquid chromatography-mass spectrometry (LC-MS) Penicillin G and sulbactam stock solutions at 10 mg/mL were freshly prepared in water from the corresponding high purity salts (Sigma Aldrich). .. A total of 30 μL of tupanvirus protein solution at 1 mg/mL was spiked with penicillin G and sulbactam at a final concentration of 10 μg/mL, before incubation at room temperature.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: A protein of the metallo-hydrolase/oxidoreductase superfamily with both beta-lactamase and ribonuclease activity is linked with translation in giant viruses
    Article Snippet: In order to determine kinetic parameters, initial velocities were calculated by Gen5.1 software (BioTek, Winooski, VT, USA) and obtained mean values were fitted using the Michaelis–Menten equation on Prism 6 (GraphPad Software, San Diego, CA, USA). .. Beta-lactam antibiotic degradation monitoring by liquid chromatography-mass spectrometry (LC-MS) Penicillin G and sulbactam stock solutions at 10 mg/mL were freshly prepared in water from the corresponding high purity salts (Sigma Aldrich). .. A total of 30 μL of tupanvirus protein solution at 1 mg/mL was spiked with penicillin G and sulbactam at a final concentration of 10 μg/mL, before incubation at room temperature.

    other:

    Article Title: Saturation mutagenesis of Asn152 reveals a substrate selectivity switch in P99 cephalosporinase
    Article Snippet: Ampicillin, penicillin G, oxacillin, cephalothin, and cefoxitin were purchased from Sigma.

    Incubation:

    Article Title: Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial
    Article Snippet: The invasion assay was performed using the antibiotic protection assay as previously described [ ]. .. Briefly, after the initial incubation time, cell monolayers were washed twice with PBS and incubated for an additional 1 h with medium containing 5 µg/mL penicillin G (Sigma) and 100 µg/mL of gentamicin (Gibco, Burlington, ON, Canada) in order to kill extracellular bacteria. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore penicillin g
    A fluorescent analogue of KSK120 (EC364) associates with surfaces of <t>penicillin-G-treated</t> C. trachomatis LGV-2. Penicillin-G (100 U/ml) was added to all infected cells at 18 hpi, concomitant with DMSO treatment (upper panels) or EC364 treatment (100 µM) (middle panels). EC364 treatment was also initiated at 29 hpi (lower panels). At 44 hpi, infected cell were fixed with methanol and stained with primary antibody toward MOMP (red). DAPI was used to detect bacterial and host DNA (blue). Confocal laser scanning microscopy was used to obtain images. DMSO-treated infections were used to define the background.
    Penicillin G, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin g/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin g - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    A fluorescent analogue of KSK120 (EC364) associates with surfaces of penicillin-G-treated C. trachomatis LGV-2. Penicillin-G (100 U/ml) was added to all infected cells at 18 hpi, concomitant with DMSO treatment (upper panels) or EC364 treatment (100 µM) (middle panels). EC364 treatment was also initiated at 29 hpi (lower panels). At 44 hpi, infected cell were fixed with methanol and stained with primary antibody toward MOMP (red). DAPI was used to detect bacterial and host DNA (blue). Confocal laser scanning microscopy was used to obtain images. DMSO-treated infections were used to define the background.

    Journal: mBio

    Article Title: A 2-Pyridone-Amide Inhibitor Targets the Glucose Metabolism Pathway of Chlamydia trachomatis

    doi: 10.1128/mBio.02304-14

    Figure Lengend Snippet: A fluorescent analogue of KSK120 (EC364) associates with surfaces of penicillin-G-treated C. trachomatis LGV-2. Penicillin-G (100 U/ml) was added to all infected cells at 18 hpi, concomitant with DMSO treatment (upper panels) or EC364 treatment (100 µM) (middle panels). EC364 treatment was also initiated at 29 hpi (lower panels). At 44 hpi, infected cell were fixed with methanol and stained with primary antibody toward MOMP (red). DAPI was used to detect bacterial and host DNA (blue). Confocal laser scanning microscopy was used to obtain images. DMSO-treated infections were used to define the background.

    Article Snippet: Penicillin-G was dissolved in water to reach a concentration of 80,000 U/ml and filter sterilized (Millipore) (0.22-µm-pore-size filter).

    Techniques: Infection, Staining, Confocal Laser Scanning Microscopy

    Antibacterial activity of the T. chebula fruit extracts against P. mirabilis (ATCC: 21721) measured as zones of inhibition (mm). M = methanolic extract; W = aqueous extract; E = ethyl acetate extract; C = chloroform extract; H = hexane extract. Positive controls: Pen = penicillin-G (20 μg); Nys = nystatin (100 Units); Cip = ciprofloxacin (2.5 μg); Chl = chloramplenicol (10 μg). Negative control (NC) = water. Results are expressed as mean zones of inhibition of at least six replicates (two repeats) ± SEM. Asterisk indicates results that are significantly different to the negative control ( p

    Journal: Indian Journal of Microbiology

    Article Title: Terminalia chebula Retz. Fruit Extracts Inhibit Bacterial Triggers of Some Autoimmune Diseases and Potentiate the Activity of Tetracycline

    doi: 10.1007/s12088-018-0754-9

    Figure Lengend Snippet: Antibacterial activity of the T. chebula fruit extracts against P. mirabilis (ATCC: 21721) measured as zones of inhibition (mm). M = methanolic extract; W = aqueous extract; E = ethyl acetate extract; C = chloroform extract; H = hexane extract. Positive controls: Pen = penicillin-G (20 μg); Nys = nystatin (100 Units); Cip = ciprofloxacin (2.5 μg); Chl = chloramplenicol (10 μg). Negative control (NC) = water. Results are expressed as mean zones of inhibition of at least six replicates (two repeats) ± SEM. Asterisk indicates results that are significantly different to the negative control ( p

    Article Snippet: Penicillin-G (potency of 1440–1680 µg/mg), chloramphenicol (≥ 98% purity by HPLC, erythromycin (potency ≥ 850 µg/mg), gentamicin (potency of 600 µg/mg), and tetracycline (≥ 95% purity by HPLC) were purchased from Sigma-Aldrich, Australia and were used as controls for the microplate liquid dilution assay.

    Techniques: Activity Assay, Inhibition, Negative Control

    Antibacterial activity of the T. chebula fruit extracts against K. pneumoniae (ATCC: 39324) measured as zones of inhibition (mm). M = methanolic extract; W = aqueous extract; E = ethyl acetate extract; C = chloroform extract; H = hexane extract. Positive controls: Pen = penicillin-G (20 μg); Nys = nystatin (100 Units); Cip = ciprofloxacin (2.5 μg); Chl = chloramplenicol (10 μg). Negative control (NC) = water. Results are expressed as mean zones of inhibition of at least six replicates (two repeats) ± SEM. Asterisk indicates results that are significantly different to the negative control ( p

    Journal: Indian Journal of Microbiology

    Article Title: Terminalia chebula Retz. Fruit Extracts Inhibit Bacterial Triggers of Some Autoimmune Diseases and Potentiate the Activity of Tetracycline

    doi: 10.1007/s12088-018-0754-9

    Figure Lengend Snippet: Antibacterial activity of the T. chebula fruit extracts against K. pneumoniae (ATCC: 39324) measured as zones of inhibition (mm). M = methanolic extract; W = aqueous extract; E = ethyl acetate extract; C = chloroform extract; H = hexane extract. Positive controls: Pen = penicillin-G (20 μg); Nys = nystatin (100 Units); Cip = ciprofloxacin (2.5 μg); Chl = chloramplenicol (10 μg). Negative control (NC) = water. Results are expressed as mean zones of inhibition of at least six replicates (two repeats) ± SEM. Asterisk indicates results that are significantly different to the negative control ( p

    Article Snippet: Penicillin-G (potency of 1440–1680 µg/mg), chloramphenicol (≥ 98% purity by HPLC, erythromycin (potency ≥ 850 µg/mg), gentamicin (potency of 600 µg/mg), and tetracycline (≥ 95% purity by HPLC) were purchased from Sigma-Aldrich, Australia and were used as controls for the microplate liquid dilution assay.

    Techniques: Activity Assay, Inhibition, Negative Control

    Phagocytosis of  S. suis  strains by THP-1 human macrophages in presence of complement-rich serum. Bacteria (1 × 10 7  CFU/mL) were incubated for 90 min with cells (MOI = 100) in presence of human serum, followed by gentamicin/penicillin G treatment to kill any remaining extracellular bacteria after incubation. Intracellular counts were done after three washes and cell lysis with water. Results represent the mean (CFU/mL) ± SEM of four independent experiments. There were no statistical differences between the  S. suis  wild-type and any of the factor H-binding protein mutants. The non-encapsulated mutant (positive control) was significantly more phagocytosed as determined by one-way ANOVA (**  p

    Journal: Pathogens

    Article Title: Recruitment of Factor H to the Streptococcus suis Cell Surface is Multifactorial

    doi: 10.3390/pathogens5030047

    Figure Lengend Snippet: Phagocytosis of S. suis strains by THP-1 human macrophages in presence of complement-rich serum. Bacteria (1 × 10 7 CFU/mL) were incubated for 90 min with cells (MOI = 100) in presence of human serum, followed by gentamicin/penicillin G treatment to kill any remaining extracellular bacteria after incubation. Intracellular counts were done after three washes and cell lysis with water. Results represent the mean (CFU/mL) ± SEM of four independent experiments. There were no statistical differences between the S. suis wild-type and any of the factor H-binding protein mutants. The non-encapsulated mutant (positive control) was significantly more phagocytosed as determined by one-way ANOVA (** p

    Article Snippet: Briefly, after the initial incubation time, cell monolayers were washed twice with PBS and incubated for an additional 1 h with medium containing 5 µg/mL penicillin G (Sigma) and 100 µg/mL of gentamicin (Gibco, Burlington, ON, Canada) in order to kill extracellular bacteria.

    Techniques: Incubation, Lysis, Binding Assay, Mutagenesis, Positive Control