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TaKaRa pegfp n2 vector
Pegfp N2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pegfp n2 vector/product/TaKaRa
Average 92 stars, based on 48 article reviews
Price from $9.99 to $1999.99
pegfp n2 vector - by Bioz Stars, 2020-08
92/100 stars

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Clone Assay:

Article Title: Receptor Tyrosine Phosphatases Guide Vertebrate Motor Axons during Development
Article Snippet: .. The entire extracellular domain, transmembrane region, and juxtamembrane region were cloned into the pEGFP-N2 vector (Clontech, Palo Alto, CA) in-frame with the GFP-coding sequence. .. This cPTPRO-GFP construct was released from the pEGFP vector and cloned into a modified pIRES plasmid with a β-actin promoter ( ).

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Amplification:

Article Title: Control of total GFP expression by alterations to the 3? region nucleotide sequence
Article Snippet: .. We amplified the gfp region of pEGFP-N2 vector (Clontech) using forward primers containing the Nde I cleavage site (CAT) (Additional file : Table S1) and the above reverse primers containing the Xho I restriction site (Additional file : Table S2). .. The amplified DNA fragment was cloned into a TA cloning vector, the entire Nde I-Xho I fragment of which was then subcloned into pET-22b(+) by replacing the pel signal sequence and polylinker as described previously [ ].

Article Title: Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3
Article Snippet: .. Enhanced GFP was amplified from the pEGFP-N2 vector (CLONTECH Laboratories, Inc.) using the primers GFP pLenti UP (5′-CACCATGGTGAGCAAGGGCGAGG-3′) and GFP pLenti DN (5′-AGTCTAGATTACTTGTACAGCT-CGTCCATGC-3′). .. The sequence encoding a modified mRFP was amplified from the vector pRSETB ( ) using the primers mRFP pLenti UP (5′-CACCATGGCCTCCTCCGAGGACGTC-3′) and mRFP pLenti DN (5′-AGTTAGGCGCCGGTGGAGTGGCG-3′).

Article Title: Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization
Article Snippet: .. Green fluorescent protein (GFP)-HBVP lines were prepared as follows: Enhanced GFP was amplified from the pEGFP-N2 vector (CLONTECH) using upstream primer (UP): (5′-CACCATGGTGAGCAAGGGCGAGG-3′) and the downstream primer (DN): (5′-AGTCTAGATTACTTGTACAGCTCGTCCATGC-3′). .. The insert was cloned into the pLenti6/V5 TOPO vector (Invitrogen).

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Article Title: Self-cleavage of fusion protein in vivo using TEV protease to yield native protein
Article Snippet: .. The cDNA of EGFP was amplified by PCR from the pEGFP-N2 vector (Clontech). ..

Construct:

Article Title: Cold-induced disruption of Na+ channel slow inactivation underlies paralysis in highly thermosensitive paramyotonia
Article Snippet: .. The human SCN4A cDNA construct was subcloned into the pEGFP-N2 vector (Clontech) for expression in mammalian cells. .. The DNA sequence for EGFP was in frame to the 3′-terminal end of the SCN4A sequence immediately before the stop codon.

Sequencing:

Article Title: Conservation of Complex Nuclear Localization Signals Utilizing Classical and Non-Classical Nuclear Import Pathways in LANA Homologs of KSHV and RFHV
Article Snippet: .. Construction of LANA NLS-EGFP fusion vectors To test the ability of different LANA peptides to function in nuclear localization, oligonucleotides encoding different putative nuclear localization signals were inserted upstream of the sequence encoding EGFP in the pEGFP-N2 vector (Clontech). .. The plasmids were constructed by annealing pairs of oligonucleotides (labeled a and b) for each test construct (see ).

Article Title: Receptor Tyrosine Phosphatases Guide Vertebrate Motor Axons during Development
Article Snippet: .. The entire extracellular domain, transmembrane region, and juxtamembrane region were cloned into the pEGFP-N2 vector (Clontech, Palo Alto, CA) in-frame with the GFP-coding sequence. .. This cPTPRO-GFP construct was released from the pEGFP vector and cloned into a modified pIRES plasmid with a β-actin promoter ( ).

CTG Assay:

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Cell Culture:

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Expressing:

Article Title: Cold-induced disruption of Na+ channel slow inactivation underlies paralysis in highly thermosensitive paramyotonia
Article Snippet: .. The human SCN4A cDNA construct was subcloned into the pEGFP-N2 vector (Clontech) for expression in mammalian cells. .. The DNA sequence for EGFP was in frame to the 3′-terminal end of the SCN4A sequence immediately before the stop codon.

Polymerase Chain Reaction:

Article Title: Self-cleavage of fusion protein in vivo using TEV protease to yield native protein
Article Snippet: .. The cDNA of EGFP was amplified by PCR from the pEGFP-N2 vector (Clontech). ..

Chloramphenicol Acetyltransferase Assay:

Article Title: Control of total GFP expression by alterations to the 3? region nucleotide sequence
Article Snippet: .. We amplified the gfp region of pEGFP-N2 vector (Clontech) using forward primers containing the Nde I cleavage site (CAT) (Additional file : Table S1) and the above reverse primers containing the Xho I restriction site (Additional file : Table S2). .. The amplified DNA fragment was cloned into a TA cloning vector, the entire Nde I-Xho I fragment of which was then subcloned into pET-22b(+) by replacing the pel signal sequence and polylinker as described previously [ ].

Cellular Antioxidant Activity Assay:

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Activated Clotting Time Assay:

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Plasmid Preparation:

Article Title: Conservation of Complex Nuclear Localization Signals Utilizing Classical and Non-Classical Nuclear Import Pathways in LANA Homologs of KSHV and RFHV
Article Snippet: .. Construction of LANA NLS-EGFP fusion vectors To test the ability of different LANA peptides to function in nuclear localization, oligonucleotides encoding different putative nuclear localization signals were inserted upstream of the sequence encoding EGFP in the pEGFP-N2 vector (Clontech). .. The plasmids were constructed by annealing pairs of oligonucleotides (labeled a and b) for each test construct (see ).

Article Title: Control of total GFP expression by alterations to the 3? region nucleotide sequence
Article Snippet: .. We amplified the gfp region of pEGFP-N2 vector (Clontech) using forward primers containing the Nde I cleavage site (CAT) (Additional file : Table S1) and the above reverse primers containing the Xho I restriction site (Additional file : Table S2). .. The amplified DNA fragment was cloned into a TA cloning vector, the entire Nde I-Xho I fragment of which was then subcloned into pET-22b(+) by replacing the pel signal sequence and polylinker as described previously [ ].

Article Title: Receptor Tyrosine Phosphatases Guide Vertebrate Motor Axons during Development
Article Snippet: .. The entire extracellular domain, transmembrane region, and juxtamembrane region were cloned into the pEGFP-N2 vector (Clontech, Palo Alto, CA) in-frame with the GFP-coding sequence. .. This cPTPRO-GFP construct was released from the pEGFP vector and cloned into a modified pIRES plasmid with a β-actin promoter ( ).

Article Title: Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3
Article Snippet: .. Enhanced GFP was amplified from the pEGFP-N2 vector (CLONTECH Laboratories, Inc.) using the primers GFP pLenti UP (5′-CACCATGGTGAGCAAGGGCGAGG-3′) and GFP pLenti DN (5′-AGTCTAGATTACTTGTACAGCT-CGTCCATGC-3′). .. The sequence encoding a modified mRFP was amplified from the vector pRSETB ( ) using the primers mRFP pLenti UP (5′-CACCATGGCCTCCTCCGAGGACGTC-3′) and mRFP pLenti DN (5′-AGTTAGGCGCCGGTGGAGTGGCG-3′).

Article Title: Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization
Article Snippet: .. Green fluorescent protein (GFP)-HBVP lines were prepared as follows: Enhanced GFP was amplified from the pEGFP-N2 vector (CLONTECH) using upstream primer (UP): (5′-CACCATGGTGAGCAAGGGCGAGG-3′) and the downstream primer (DN): (5′-AGTCTAGATTACTTGTACAGCTCGTCCATGC-3′). .. The insert was cloned into the pLenti6/V5 TOPO vector (Invitrogen).

Article Title: A Novel Germ Cell-specific Protein, SHIP1, Forms a Complex with Chromatin Remodeling Activity during Spermatogenesis *
Article Snippet: .. Cloning and Cell Culture —Mm.290718 cDNA sequences were amplified from mouse testis cDNA using the primers forward, 5′-GGA AGC TTG ATG GAG CCA TAC AGT-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA AGG GC-3′, incorporating 5′ HindIII and 3′ SalI sites, and cloned into the pEGFP-N2 vector (Clontech). .. The zinc finger domain, first, second, and third regions, ELM2, and/or SANT domain were amplified from mouse testis cDNA using the following primers: first region: forward, 5′-GCG TCG ACC GAT GGA GCC ATA CAG T-3′, and reverse, 5′-TAG GAT CCC TGA TGG GAC AGA GT-3′; second region: forward, 5′-TCG AAT TCA TGT CAG GAT CCC AGC C-3′, and reverse, 5′-AAG TCG ACA AGG AGA GTG CAG GCA G-3′; third region: forward, 5′-TCG AAT TCA TGC CAG GAG GGT GCC A-3′, and reverse, 5′-ATG TCG ACT CCA CTG CAA GGG GCC C-3′; zinc finger domain: forward, 5′-GCG AAT TCA TGC TGT GTG GGA AA-3′, and reverse, 5′-TCG GAT CCT CAG GAT GCA TAC TC-3′; ELM2 domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-TTG GAT CCT CAG GTT GGT GCC C-3′; SANT domain: forward, 5′-GGA ATT CGA TGC GAT ACA CAG GT-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; ELM2-SANT domain: forward, 5′-CCA GAA TTC GAT GCC ACA TAT CAA-3′, and reverse, 5′-CGG ATC CTC ATC TTT TTC CAG ATG-3′; and subcloned into pEGFP-N2.

Article Title: Cold-induced disruption of Na+ channel slow inactivation underlies paralysis in highly thermosensitive paramyotonia
Article Snippet: .. The human SCN4A cDNA construct was subcloned into the pEGFP-N2 vector (Clontech) for expression in mammalian cells. .. The DNA sequence for EGFP was in frame to the 3′-terminal end of the SCN4A sequence immediately before the stop codon.

Article Title: Self-cleavage of fusion protein in vivo using TEV protease to yield native protein
Article Snippet: .. The cDNA of EGFP was amplified by PCR from the pEGFP-N2 vector (Clontech). ..

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  • 89
    TaKaRa pegfp n2 plasmid
    Efficiency, viability, and yields of transfection of the <t>pEGFP-N2</t> plasmid into bovine fibroblasts by droplet-EP. A total of 4 × 10 5 bovine earlobe fibroblasts were subjected to droplet- electroporation (EP) at the following settings: 2.0, 3.0, 3.5, or 4.0 kV. Cell viability was determined immediately after charging the cells through droplet-EP. Subsequently, the transfected cells were cultured for 24 hr. After staining the nuclei with Hoechst33342, the transfection efficiency was determined using a laser-scanning microscope. (A) Transfection efficiency was determined based on the proportion of enhanced green fluorescent protein (EGFP)-expressing cells in Hoechst33342-positive cells. (B) A representative image of EGFP-transfected cells. Scale bar=50 µ m. (C) Cell viability was determined using the trypan blue staining method. (D) The yields of transfections were determined by multiplying the efficiency and cell viability obtained at each voltage. “Intact” indicates that droplet-EP was not performed. Values are means ± one standard deviation over three wells. * P
    Pegfp N2 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pegfp n2 plasmid/product/TaKaRa
    Average 89 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    pegfp n2 plasmid - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    90
    TaKaRa expression green fluorescence protein n2
    Characterization of the Foxp3 recombinant plasmids. Notes: Lane C, plasmid pEGFP-N2, used as transfection control; the recombinant plasmids siFoxp3-1, siFoxp3-2, and siFoxp3-3 were digested with the enzyme HindIII. Lane M shows the molecular weight marker DL 10000 DNA ladder. Abbreviations: Lane C, plasmid pEGFP-N2 used as transfection control; Lane M, molecular weight marker; siFoxp3, the recombinant plasmids 1–3; pEGFP-N2, plasmid of expression green fluorescence <t>protein-N2.</t>
    Expression Green Fluorescence Protein N2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression green fluorescence protein n2/product/TaKaRa
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    expression green fluorescence protein n2 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    TaKaRa cytomegalovirus early promoter pegfp n2
    Transmission electron micrograph of <t>ORMOSIL/pEGFP-N2</t> nanoparticles.
    Cytomegalovirus Early Promoter Pegfp N2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytomegalovirus early promoter pegfp n2/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytomegalovirus early promoter pegfp n2 - by Bioz Stars, 2020-08
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      Buy from Supplier

    85
    TaKaRa fluorescent protein egfp expression vector pegfp n2
    Fractionation of ATZ-GFP-containing IBs and ATZ-containing globules. A , panel i , Hepa 1-6 cells were transiently transfected with <t>AAT-pEGFP-N2</t> or ATZ-pEGFP-N2 and analyzed by immunofluorescence microscopy 48 h after transfection. Cells were stained with
    Fluorescent Protein Egfp Expression Vector Pegfp N2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent protein egfp expression vector pegfp n2/product/TaKaRa
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fluorescent protein egfp expression vector pegfp n2 - by Bioz Stars, 2020-08
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    Efficiency, viability, and yields of transfection of the pEGFP-N2 plasmid into bovine fibroblasts by droplet-EP. A total of 4 × 10 5 bovine earlobe fibroblasts were subjected to droplet- electroporation (EP) at the following settings: 2.0, 3.0, 3.5, or 4.0 kV. Cell viability was determined immediately after charging the cells through droplet-EP. Subsequently, the transfected cells were cultured for 24 hr. After staining the nuclei with Hoechst33342, the transfection efficiency was determined using a laser-scanning microscope. (A) Transfection efficiency was determined based on the proportion of enhanced green fluorescent protein (EGFP)-expressing cells in Hoechst33342-positive cells. (B) A representative image of EGFP-transfected cells. Scale bar=50 µ m. (C) Cell viability was determined using the trypan blue staining method. (D) The yields of transfections were determined by multiplying the efficiency and cell viability obtained at each voltage. “Intact” indicates that droplet-EP was not performed. Values are means ± one standard deviation over three wells. * P

    Journal: The Journal of Veterinary Medical Science

    Article Title: Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation

    doi: 10.1292/jvms.19-0475

    Figure Lengend Snippet: Efficiency, viability, and yields of transfection of the pEGFP-N2 plasmid into bovine fibroblasts by droplet-EP. A total of 4 × 10 5 bovine earlobe fibroblasts were subjected to droplet- electroporation (EP) at the following settings: 2.0, 3.0, 3.5, or 4.0 kV. Cell viability was determined immediately after charging the cells through droplet-EP. Subsequently, the transfected cells were cultured for 24 hr. After staining the nuclei with Hoechst33342, the transfection efficiency was determined using a laser-scanning microscope. (A) Transfection efficiency was determined based on the proportion of enhanced green fluorescent protein (EGFP)-expressing cells in Hoechst33342-positive cells. (B) A representative image of EGFP-transfected cells. Scale bar=50 µ m. (C) Cell viability was determined using the trypan blue staining method. (D) The yields of transfections were determined by multiplying the efficiency and cell viability obtained at each voltage. “Intact” indicates that droplet-EP was not performed. Values are means ± one standard deviation over three wells. * P

    Article Snippet: Enhanced green fluorescent protein (EGFP)-encoding plasmid The pEGFP-N2 plasmid was purchased from Takara Bio (Kusatsu, Japan).

    Techniques: Transfection, Plasmid Preparation, Electroporation, Cell Culture, Staining, Laser-Scanning Microscopy, Expressing, Standard Deviation

    Efficiency and cytotoxicity of transfection of the pEGFP-N2 plasmid into swine fibroblasts by droplet-electroporation (EP). Swine fibroblasts were isolated from earlobe skin, and subjected to transfection by droplet-EP. (A) Morphology of swine earlobe fibroblasts. Scale bar=50 µ m. (B–D) Transfection efficiency (B), cell viability (C), and yields of transfection (D) were determined (as shown in Fig. 1 ). Values are means ± one standard deviation over three wells. * P

    Journal: The Journal of Veterinary Medical Science

    Article Title: Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation

    doi: 10.1292/jvms.19-0475

    Figure Lengend Snippet: Efficiency and cytotoxicity of transfection of the pEGFP-N2 plasmid into swine fibroblasts by droplet-electroporation (EP). Swine fibroblasts were isolated from earlobe skin, and subjected to transfection by droplet-EP. (A) Morphology of swine earlobe fibroblasts. Scale bar=50 µ m. (B–D) Transfection efficiency (B), cell viability (C), and yields of transfection (D) were determined (as shown in Fig. 1 ). Values are means ± one standard deviation over three wells. * P

    Article Snippet: Enhanced green fluorescent protein (EGFP)-encoding plasmid The pEGFP-N2 plasmid was purchased from Takara Bio (Kusatsu, Japan).

    Techniques: Transfection, Plasmid Preparation, Electroporation, Isolation, Standard Deviation

    Characterization of the Foxp3 recombinant plasmids. Notes: Lane C, plasmid pEGFP-N2, used as transfection control; the recombinant plasmids siFoxp3-1, siFoxp3-2, and siFoxp3-3 were digested with the enzyme HindIII. Lane M shows the molecular weight marker DL 10000 DNA ladder. Abbreviations: Lane C, plasmid pEGFP-N2 used as transfection control; Lane M, molecular weight marker; siFoxp3, the recombinant plasmids 1–3; pEGFP-N2, plasmid of expression green fluorescence protein-N2.

    Journal: OncoTargets and therapy

    Article Title: Silencing of Foxp3 delays the growth of murine melanomas and modifies the tumor immunosuppressive environment

    doi: 10.2147/OTT.S90476

    Figure Lengend Snippet: Characterization of the Foxp3 recombinant plasmids. Notes: Lane C, plasmid pEGFP-N2, used as transfection control; the recombinant plasmids siFoxp3-1, siFoxp3-2, and siFoxp3-3 were digested with the enzyme HindIII. Lane M shows the molecular weight marker DL 10000 DNA ladder. Abbreviations: Lane C, plasmid pEGFP-N2 used as transfection control; Lane M, molecular weight marker; siFoxp3, the recombinant plasmids 1–3; pEGFP-N2, plasmid of expression green fluorescence protein-N2.

    Article Snippet: The oligos were resuspended to a final concentration of 1 µg/µL−1 , annealed, and ligated into plasmid of expression green fluorescence protein-N2 (pEGFP-N2; Clontech, Palo Alto, CA, USA).

    Techniques: Recombinant, Plasmid Preparation, Transfection, Molecular Weight, Marker, Expressing, Fluorescence

    Transmission electron micrograph of ORMOSIL/pEGFP-N2 nanoparticles.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

    doi: 10.1073/pnas.0504926102

    Figure Lengend Snippet: Transmission electron micrograph of ORMOSIL/pEGFP-N2 nanoparticles.

    Article Snippet: Plasmid expressing EGFP with the cytomegalovirus early promoter (pEGFP-N2) and mAb to EGFP were purchased from Clontech.

    Techniques: Transmission Assay

    Expression of EGFP in multiple brain areas after injection of ORMOSIL-pEGFP-N2 into the brain LV. Brain sections were immunostained with EGFP antibodies as described in Materials and Methods .( A and B ) Control ORMOSIL nanoparticles. ( A ) The region surrounding the LV. Str, striatum; Sep, septum; cc, corpus callosum. ( B ) The hippocampal region adjacent to the ventricle. No cellular staining is detected in either region by using anti-EGFP immunocytochemistry. ( C – F ) ORMOSIL/pEGFP-N2 particles. Injection resulted in EGFP immunostaining of the neuron-shaped cells in dorsal lateral (d), lateral (l), and medial (m) septal nuclei ( C ); in the adjacent striatal region of the brain ( D ); cingulate and motor cortex ( E ); and pyramidal neurons of the CA3 hippocampal region ( F ).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

    doi: 10.1073/pnas.0504926102

    Figure Lengend Snippet: Expression of EGFP in multiple brain areas after injection of ORMOSIL-pEGFP-N2 into the brain LV. Brain sections were immunostained with EGFP antibodies as described in Materials and Methods .( A and B ) Control ORMOSIL nanoparticles. ( A ) The region surrounding the LV. Str, striatum; Sep, septum; cc, corpus callosum. ( B ) The hippocampal region adjacent to the ventricle. No cellular staining is detected in either region by using anti-EGFP immunocytochemistry. ( C – F ) ORMOSIL/pEGFP-N2 particles. Injection resulted in EGFP immunostaining of the neuron-shaped cells in dorsal lateral (d), lateral (l), and medial (m) septal nuclei ( C ); in the adjacent striatal region of the brain ( D ); cingulate and motor cortex ( E ); and pyramidal neurons of the CA3 hippocampal region ( F ).

    Article Snippet: Plasmid expressing EGFP with the cytomegalovirus early promoter (pEGFP-N2) and mAb to EGFP were purchased from Clontech.

    Techniques: Expressing, Injection, Staining, Immunocytochemistry, Immunostaining

    ORMOSIL nanoparticle transfection in the SNc. ( A ) DNA-free ORMOSIL injection showing no substantial immunostaining for EGFP. ( B – E ) Injection of ORMOSIL-pEGFP-N2 complex into SNc. ( B ) Multiple cells with typical dopaminergic neuron morphology are immunostained positive for EGFP. ( C ) No immunostaining is observed without primary anti-EGFP Ab. ( D ) EGFP immunostaining of neuron-shaped cells (higher magnification). ( E ) Transfected EGFP (green) is expressed in TH-immunopositive (red) dopaminergic neuron.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

    doi: 10.1073/pnas.0504926102

    Figure Lengend Snippet: ORMOSIL nanoparticle transfection in the SNc. ( A ) DNA-free ORMOSIL injection showing no substantial immunostaining for EGFP. ( B – E ) Injection of ORMOSIL-pEGFP-N2 complex into SNc. ( B ) Multiple cells with typical dopaminergic neuron morphology are immunostained positive for EGFP. ( C ) No immunostaining is observed without primary anti-EGFP Ab. ( D ) EGFP immunostaining of neuron-shaped cells (higher magnification). ( E ) Transfected EGFP (green) is expressed in TH-immunopositive (red) dopaminergic neuron.

    Article Snippet: Plasmid expressing EGFP with the cytomegalovirus early promoter (pEGFP-N2) and mAb to EGFP were purchased from Clontech.

    Techniques: Transfection, Injection, Immunostaining

    Transfection of ORMOSIL-pEGFP-N2 complex into the LV cells of the SVZ. Mice were transfected with ORMOSIL/pEGFP-N2 by injection into the brain LV. ( A and B ) Seven days postmortem EGFP immunostaining is shown at low magnification ( A ) and at higher magnification ( B ) of the positive region to visualize transfected cells. ( C and D ) In vivo imaging of EGFP fluorescence in cells in the LV. Ten days after transfection, mice were subjected to the second stereotaxic surgery, and a miniature fiber-optic Cell-viZio probe was inserted into the anterior dorsal region ( C ) or the posterior region ( D on the PNAS web site.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain

    doi: 10.1073/pnas.0504926102

    Figure Lengend Snippet: Transfection of ORMOSIL-pEGFP-N2 complex into the LV cells of the SVZ. Mice were transfected with ORMOSIL/pEGFP-N2 by injection into the brain LV. ( A and B ) Seven days postmortem EGFP immunostaining is shown at low magnification ( A ) and at higher magnification ( B ) of the positive region to visualize transfected cells. ( C and D ) In vivo imaging of EGFP fluorescence in cells in the LV. Ten days after transfection, mice were subjected to the second stereotaxic surgery, and a miniature fiber-optic Cell-viZio probe was inserted into the anterior dorsal region ( C ) or the posterior region ( D on the PNAS web site.

    Article Snippet: Plasmid expressing EGFP with the cytomegalovirus early promoter (pEGFP-N2) and mAb to EGFP were purchased from Clontech.

    Techniques: Transfection, Mouse Assay, Injection, Immunostaining, In Vivo Imaging, Fluorescence

    Fractionation of ATZ-GFP-containing IBs and ATZ-containing globules. A , panel i , Hepa 1-6 cells were transiently transfected with AAT-pEGFP-N2 or ATZ-pEGFP-N2 and analyzed by immunofluorescence microscopy 48 h after transfection. Cells were stained with

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: Fractionation of ATZ-GFP-containing IBs and ATZ-containing globules. A , panel i , Hepa 1-6 cells were transiently transfected with AAT-pEGFP-N2 or ATZ-pEGFP-N2 and analyzed by immunofluorescence microscopy 48 h after transfection. Cells were stained with

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Fractionation, Transfection, Immunofluorescence, Microscopy, Staining

    ATZ degradation requires HRD1 in absence and presence of ATF6 activation. A , panel i , Hepa 1-6 cells were transiently transfected with ATZ-pEGFP-N2 and either siRNA negative control or siRNA against mouse HRD1. Cell lysates were analyzed by Western blot

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: ATZ degradation requires HRD1 in absence and presence of ATF6 activation. A , panel i , Hepa 1-6 cells were transiently transfected with ATZ-pEGFP-N2 and either siRNA negative control or siRNA against mouse HRD1. Cell lysates were analyzed by Western blot

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Activation Assay, Transfection, Negative Control, Western Blot

    ATZ is degraded shortly after synthesis. A , panel i , Hepa 1-6 cells transiently transfected with ATZ-pEGFP-N2 and ATF6-HA-pCGN or ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN were pulsed with 150 μCi/ml [ 35 S]Met and [ 35 S]Cys. Cells were chased

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: ATZ is degraded shortly after synthesis. A , panel i , Hepa 1-6 cells transiently transfected with ATZ-pEGFP-N2 and ATF6-HA-pCGN or ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN were pulsed with 150 μCi/ml [ 35 S]Met and [ 35 S]Cys. Cells were chased

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Transfection

    Expression of ATF6(1–373) reduces expression of ATZ and formation of IBs. A , panel i , cells were transiently transfected with ATZ-pEGFP-N2 and ATF6-HA-pCGN or with ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN and analyzed by immunofluorescence.

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: Expression of ATF6(1–373) reduces expression of ATZ and formation of IBs. A , panel i , cells were transiently transfected with ATZ-pEGFP-N2 and ATF6-HA-pCGN or with ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN and analyzed by immunofluorescence.

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Expressing, Transfection, Immunofluorescence

    ATF6(1–373) expression does not induce XBP1 and ATF4 or promote apoptosis. A , Hepa 1-6 cells were transiently transfected with ATZ-pEGFP-N2 and either ATF6-HA-pCGN or ATF6(1–373)-HA-pCGN. As a positive control for ER stress, cells were

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: ATF6(1–373) expression does not induce XBP1 and ATF4 or promote apoptosis. A , Hepa 1-6 cells were transiently transfected with ATZ-pEGFP-N2 and either ATF6-HA-pCGN or ATF6(1–373)-HA-pCGN. As a positive control for ER stress, cells were

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Expressing, Transfection, Positive Control

    Expression of ATF6(1–373) increases degradation of ATZ by proteasome. A , Hepa 1-6 cells were transiently transfected with either ATZ-pEGFP-N2 and ATF6-HA-pCGN or ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN. After 48 h, cells were incubated in

    Journal: The Journal of Biological Chemistry

    Article Title: Activating Transcription Factor 6 Limits Intracellular Accumulation of Mutant ?1-Antitrypsin Z and Mitochondrial Damage in Hepatoma Cells *

    doi: 10.1074/jbc.M111.280073

    Figure Lengend Snippet: Expression of ATF6(1–373) increases degradation of ATZ by proteasome. A , Hepa 1-6 cells were transiently transfected with either ATZ-pEGFP-N2 and ATF6-HA-pCGN or ATZ-pEGFP-N2 and ATF6(1–373)-HA-pCGN. After 48 h, cells were incubated in

    Article Snippet: The enhanced green fluorescent protein (EGFP) expression vector pEGFP-N2 was purchased from Clontech.

    Techniques: Expressing, Transfection, Incubation