pe labeled mouse anti rat igg1  (SouthernBiotech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Mouse Anti Rat IgG1 PE
    Description:

    Catalog Number:
    3060-09
    Price:
    None
    Applications:
    Applications for relevant formats of this clone include -Flow Cytometry – Quality tested 1-5ELISA – Quality tested 6,7FLISA – Quality testedImmunohistochemistry-Frozen Sections 8
    Format:
    PE (R-phycoerythrin)
    Isotype:
    Mouse (BALB/c) IgG1κ
    Buy from Supplier


    Structured Review

    SouthernBiotech pe labeled mouse anti rat igg1
    Mouse Anti Rat IgG1 PE

    https://www.bioz.com/result/pe labeled mouse anti rat igg1/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe labeled mouse anti rat igg1 - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Activation of rheumatoid factor-specific B cells is antigen-dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model"

    Article Title: Activation of rheumatoid factor-specific B cells is antigen-dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1303000

    The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a
    Figure Legend Snippet: The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a

    Techniques Used: Mouse Assay

    Related Articles

    Incubation:

    Article Title: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection
    Article Snippet: Cells were then washed and stained with anti-LAG-3 mAb (71-2D8, rat IgG1 ) [ ] or rat IgG1 isotype control (R3-34, BD Biosciences) for 20 min at room temperature. .. Finally, cells were washed and incubated with anti-CD4-FITC mAb (CC8; Bio-Rad, Hercules, CA, USA) or anti-CD8-FITC mAb (CC63, Bio-Rad) in the presence of anti-TCR1-N24-APC/Cy7 mAb (anti-TCR δ chain; GB21A; Washington State University Monoclonal Antibody Center, Pullman, WA, USA), anti-CD3-PerCp/Cy5.5 mAb (MM1A; Washington State University Monoclonal Antibody Center), anti-IgM-PE/Cy7 mAb (IL-A30; Bio-Rad), and PE-conjugated anti-rat IgG1 mAb (G17E7; Southern Biotech) for 20 min at room temperature. mAbs GB21A, MM1A, and IL-A30 were conjugated with APC/Cy7, PerCp/Cy5.5, and PE/Cy7, respectively, using Lightning-Link Conjugation Kits (Innova Biosciences, Cambridge, England, UK). .. Cells were then washed and immediately analyzed by FACS Verse (BD Biosciences) and FCS Express 4 (De Novo Software, Glendale, CA, USA).

    Article Title: Trib1 Is Overexpressed in Systemic Lupus Erythematosus, While It Regulates Immunoglobulin Production in Murine B Cells
    Article Snippet: After a washing step, cells were fixed with 100 µL of fixation buffer from Fixation/Permeabilization kit (eBioscience) during 20 min at room temperature, in the dark. .. Cells were then permeabilized with the permeabilization buffer from Fixation/Permeabilization kit (eBioscience) and incubated for 30 min at room temperature in the dark with anti-mouse IgG1 (PE, Southern Biotech) and washed before acquisition by a cytometer. .. For all stainings, cells were analyzed using the FACS Calibur (BD Biosciences).

    Article Title: Efficient generation of a monoclonal antibody against the human C-type lectin receptor DCIR by targeting murine dendritic cells
    Article Snippet: The isotype controls IgG1, IgG2a, goat serum as well as the antibody clones I3-612 (Becton Dickinson), 216110 (R & D Systems), and the polyclonal hDCIR antibody (R & D Systems) were either bought or labeled with Alexa-647 (Invitrogen/Molecular Probes). .. To analyze 293T cells transiently transfected with hDCIR-HA or hDCIR (Lipofectamin, Invitrogen and Fugene, Roche), hybridoma antibody supernatants were incubated with the transfected 293T cells, washed and further incubated with anti mouse whole IgG PE antibody (Southern Biotech). .. In some experiments the cells were stained with anti HA-FITC antibody (Roche).

    Conjugation Assay:

    Article Title: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection
    Article Snippet: Cells were then washed and stained with anti-LAG-3 mAb (71-2D8, rat IgG1 ) [ ] or rat IgG1 isotype control (R3-34, BD Biosciences) for 20 min at room temperature. .. Finally, cells were washed and incubated with anti-CD4-FITC mAb (CC8; Bio-Rad, Hercules, CA, USA) or anti-CD8-FITC mAb (CC63, Bio-Rad) in the presence of anti-TCR1-N24-APC/Cy7 mAb (anti-TCR δ chain; GB21A; Washington State University Monoclonal Antibody Center, Pullman, WA, USA), anti-CD3-PerCp/Cy5.5 mAb (MM1A; Washington State University Monoclonal Antibody Center), anti-IgM-PE/Cy7 mAb (IL-A30; Bio-Rad), and PE-conjugated anti-rat IgG1 mAb (G17E7; Southern Biotech) for 20 min at room temperature. mAbs GB21A, MM1A, and IL-A30 were conjugated with APC/Cy7, PerCp/Cy5.5, and PE/Cy7, respectively, using Lightning-Link Conjugation Kits (Innova Biosciences, Cambridge, England, UK). .. Cells were then washed and immediately analyzed by FACS Verse (BD Biosciences) and FCS Express 4 (De Novo Software, Glendale, CA, USA).

    Cytometry:

    Article Title: Trib1 Is Overexpressed in Systemic Lupus Erythematosus, While It Regulates Immunoglobulin Production in Murine B Cells
    Article Snippet: After a washing step, cells were fixed with 100 µL of fixation buffer from Fixation/Permeabilization kit (eBioscience) during 20 min at room temperature, in the dark. .. Cells were then permeabilized with the permeabilization buffer from Fixation/Permeabilization kit (eBioscience) and incubated for 30 min at room temperature in the dark with anti-mouse IgG1 (PE, Southern Biotech) and washed before acquisition by a cytometer. .. For all stainings, cells were analyzed using the FACS Calibur (BD Biosciences).

    Transfection:

    Article Title: Efficient generation of a monoclonal antibody against the human C-type lectin receptor DCIR by targeting murine dendritic cells
    Article Snippet: The isotype controls IgG1, IgG2a, goat serum as well as the antibody clones I3-612 (Becton Dickinson), 216110 (R & D Systems), and the polyclonal hDCIR antibody (R & D Systems) were either bought or labeled with Alexa-647 (Invitrogen/Molecular Probes). .. To analyze 293T cells transiently transfected with hDCIR-HA or hDCIR (Lipofectamin, Invitrogen and Fugene, Roche), hybridoma antibody supernatants were incubated with the transfected 293T cells, washed and further incubated with anti mouse whole IgG PE antibody (Southern Biotech). .. In some experiments the cells were stained with anti HA-FITC antibody (Roche).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    SouthernBiotech pe labeled mouse anti rat igg1
    The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin <t>IgG2a</t> a
    Pe Labeled Mouse Anti Rat Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe labeled mouse anti rat igg1/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe labeled mouse anti rat igg1 - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    93
    SouthernBiotech anti cd25 mab
    Foxp3 + expression in freshly isolated and expanded CD4 + T reg cells. A) Foxp3 expression in fresh <t>CD25</t> + CD62L HI cells (Q2, 96%) versus CD25 + CD62L LO cells (Q4, 89%). Isotype control antibody shown for each histogram. B) Expanded Q2 cells demonstrating maintenance of CD62L HI and Foxp3 + expression (90%). C) Expanded Q4 cells demonstrating up-regulation of CD62L HI on 33% of cells and a mixture of 65% Foxp3 + T regs and 35% Foxp3 − T eff cells post-expansion. D) Foxp3 + expression (grey histogram, 85% of cells) following expansion of the entire CD4 + CD25 + T cell subset. Isotype control shown in black histogram.
    Anti Cd25 Mab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd25 mab/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd25 mab - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    88
    SouthernBiotech mouse igg2b isotype control
    Differential APM component expression in immature and mature IFN DC and IL-4 DC. (A) Permeabilized control T2 and Colo38 cells were incubated with TAP1- and TAP2-specific mAb and analyzed by flow cytometry as a control. The percentage of positive cells and MFI are reported in each panel. (B) Permeabilized immature and mature IFN DC (bold line histogram) and IL-4 DC (black filled histogram) were incubated with TAP1-specific mAb NOB1, TAP2-specific mAb NOB2, calreticulin-specific mAb TO-11, calnexin-specific mAb TO-5, and tapasin-specific mAb TO-3 and subsequently stained with FITC-conjugated goat anti-mouse <t>IgG</t> antibodies. Stained cells were analyzed by flow cytometry. Cells incubated only with FITC-labeled goat anti-mouse IgG antibodies (gray filled and broken line histogram) were used as a negative control. The percentage of positive cells and MFI are reported under each panel.
    Mouse Igg2b Isotype Control, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse igg2b isotype control/product/SouthernBiotech
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse igg2b isotype control - by Bioz Stars, 2021-06
    88/100 stars
      Buy from Supplier

    Image Search Results


    The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Activation of rheumatoid factor-specific B cells is antigen-dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model

    doi: 10.4049/jimmunol.1303000

    Figure Lengend Snippet: The production of Id + RF by TC.AM14 a mice is correlated with the secretion of anti-chromatin IgG2a a

    Article Snippet: The NIM-R6 Ab , which recognizes both CD22a and CD22b alleles ( ) was revealed with either FITC-labeled mouse anti-rat IgG1 (eBioscience) or PE-labeled mouse anti-rat-IgG1 (Southern Biotech).

    Techniques: Mouse Assay

    Selection of anti hDCIR antibodies. ELISA was performed on supernatants of (A) 246 clones from selection round one and (B) remaining 36 clones from the second selection round. Pie-charts show percentage of antibody subclasses IgM ( ), IgG (■), IgG2a (□), IgG2b ( ), IgG3 ( ) present in the individual rounds of selection. (C) 293T cells were transiently transfected with hDCIR-HA and analyzed by FACS. Dot blots show living cells which were stained with anti HA-FITC (y-axis) and hDCIR-A647 antibody (clone I3-612 from BD, x-axis, upper panel), or anti HA-FITC antibody and Alexa-647 labeled isotype control IgG1 (y-axis, lower panel). (D) ELISA positive antibody supernatants were used for FACS staining of 293T cells transiently transfected with hDCIR. As a control unspecific isotype matched control antibodies were used. Shown are dot blots on living cells. The experiment was repeated 2 times. (E) Soluble 5′His-tagged hDCIR (2 μg/lane) protein was loaded onto a 4–16% Tris-Glycine gel, blotted and developed with anti hDCIR antibody supernatants (clones 15E12, 6H10, 6B4), or unspecific antibody supernatant and HRP-conjugated anti mouse whole IgG antibodies. Western blots were developed with enhanced luminescence (Pierce). The experiment was repeated twice.

    Journal: Immunology letters

    Article Title: Efficient generation of a monoclonal antibody against the human C-type lectin receptor DCIR by targeting murine dendritic cells

    doi: 10.1016/j.imlet.2010.06.002

    Figure Lengend Snippet: Selection of anti hDCIR antibodies. ELISA was performed on supernatants of (A) 246 clones from selection round one and (B) remaining 36 clones from the second selection round. Pie-charts show percentage of antibody subclasses IgM ( ), IgG (■), IgG2a (□), IgG2b ( ), IgG3 ( ) present in the individual rounds of selection. (C) 293T cells were transiently transfected with hDCIR-HA and analyzed by FACS. Dot blots show living cells which were stained with anti HA-FITC (y-axis) and hDCIR-A647 antibody (clone I3-612 from BD, x-axis, upper panel), or anti HA-FITC antibody and Alexa-647 labeled isotype control IgG1 (y-axis, lower panel). (D) ELISA positive antibody supernatants were used for FACS staining of 293T cells transiently transfected with hDCIR. As a control unspecific isotype matched control antibodies were used. Shown are dot blots on living cells. The experiment was repeated 2 times. (E) Soluble 5′His-tagged hDCIR (2 μg/lane) protein was loaded onto a 4–16% Tris-Glycine gel, blotted and developed with anti hDCIR antibody supernatants (clones 15E12, 6H10, 6B4), or unspecific antibody supernatant and HRP-conjugated anti mouse whole IgG antibodies. Western blots were developed with enhanced luminescence (Pierce). The experiment was repeated twice.

    Article Snippet: To analyze 293T cells transiently transfected with hDCIR-HA or hDCIR (Lipofectamin, Invitrogen and Fugene, Roche), hybridoma antibody supernatants were incubated with the transfected 293T cells, washed and further incubated with anti mouse whole IgG PE antibody (Southern Biotech).

    Techniques: Selection, Enzyme-linked Immunosorbent Assay, Clone Assay, Transfection, FACS, Staining, Labeling, Western Blot

    New anti hDCIR antibody clone is highly specific to hDCIR. 293T cells were transiently transfected with hDCIR, DC-SIGN, or BDCA-2 together with a GFP expression plasmid. One day later binding of hDCIR antibody clone 15E12, as well as binding of anti DC-SIGN, and anti BDCA-2 and their isotype controls (mouse IgG1, mouse IgG2a, rat IgG2a) was tested. Shown are histogram overlays of GFP positive 293T cells. The experiment was repeated three times.

    Journal: Immunology letters

    Article Title: Efficient generation of a monoclonal antibody against the human C-type lectin receptor DCIR by targeting murine dendritic cells

    doi: 10.1016/j.imlet.2010.06.002

    Figure Lengend Snippet: New anti hDCIR antibody clone is highly specific to hDCIR. 293T cells were transiently transfected with hDCIR, DC-SIGN, or BDCA-2 together with a GFP expression plasmid. One day later binding of hDCIR antibody clone 15E12, as well as binding of anti DC-SIGN, and anti BDCA-2 and their isotype controls (mouse IgG1, mouse IgG2a, rat IgG2a) was tested. Shown are histogram overlays of GFP positive 293T cells. The experiment was repeated three times.

    Article Snippet: To analyze 293T cells transiently transfected with hDCIR-HA or hDCIR (Lipofectamin, Invitrogen and Fugene, Roche), hybridoma antibody supernatants were incubated with the transfected 293T cells, washed and further incubated with anti mouse whole IgG PE antibody (Southern Biotech).

    Techniques: Transfection, Expressing, Plasmid Preparation, Binding Assay

    Foxp3 + expression in freshly isolated and expanded CD4 + T reg cells. A) Foxp3 expression in fresh CD25 + CD62L HI cells (Q2, 96%) versus CD25 + CD62L LO cells (Q4, 89%). Isotype control antibody shown for each histogram. B) Expanded Q2 cells demonstrating maintenance of CD62L HI and Foxp3 + expression (90%). C) Expanded Q4 cells demonstrating up-regulation of CD62L HI on 33% of cells and a mixture of 65% Foxp3 + T regs and 35% Foxp3 − T eff cells post-expansion. D) Foxp3 + expression (grey histogram, 85% of cells) following expansion of the entire CD4 + CD25 + T cell subset. Isotype control shown in black histogram.

    Journal: PLoS ONE

    Article Title: Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells

    doi: 10.1371/journal.pone.0006031

    Figure Lengend Snippet: Foxp3 + expression in freshly isolated and expanded CD4 + T reg cells. A) Foxp3 expression in fresh CD25 + CD62L HI cells (Q2, 96%) versus CD25 + CD62L LO cells (Q4, 89%). Isotype control antibody shown for each histogram. B) Expanded Q2 cells demonstrating maintenance of CD62L HI and Foxp3 + expression (90%). C) Expanded Q4 cells demonstrating up-regulation of CD62L HI on 33% of cells and a mixture of 65% Foxp3 + T regs and 35% Foxp3 − T eff cells post-expansion. D) Foxp3 + expression (grey histogram, 85% of cells) following expansion of the entire CD4 + CD25 + T cell subset. Isotype control shown in black histogram.

    Article Snippet: The anti-CD25 mAb (R-PE-conjugated, 7D4) and anti-CTLA4 mAb (R-PE-conjugated, 1B8) were purchased from Southern Biotechnology Associates, Inc (Birmingham, AL).

    Techniques: Expressing, Isolation

    In vivo survival and proliferation of expanded T regs and T effs (independently injected into the same mouse) indicating impaired survival of T eff-conditioned is not intrinsic to all transferred T effs , but instead due to co-culture with T regs . A) CFSE + and SNARF-1 cells recovered four days following independent injections of expanded CFSE + CD4 + CD25 + cells (containing T regs and T eff-conditioned ) and expanded SNARF-1 + CD4 + CD25 − T effs (unconditioned T effs ) demonstrating approximately equal survival of both populations (∼0.4% of recovered lymphocytes each). The ratio of injected cells on Day 0 was 45% T regs , 10% T eff-conditioned , 45% unconditioned T effs . B) Histogram of recovered CFSE + and SNARF-1 + cells at day 4 demonstrate persistence of “unconditioned” T effs (SNARF-1 + cells) equivalent to survival of the CFSE + T reg /T eff-conditioned mix, a finding not observed when only T regs and T eff-conditioned are co-transferred (as shown in Figure 5 ). C–D) Foxp3 analysis of sorted cells demonstrating that surviving CFSE + cells are T regs (97% Foxp3 + ) and SNARF-1 + cells are “unconditioned” T effs (Foxp3 − ), indicating impaired survival and proliferation of transferred T eff-conditioned is a consequence of co-culture with T reg during exogenous expansion (characteristic findings from 5 mice).

    Journal: PLoS ONE

    Article Title: Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells

    doi: 10.1371/journal.pone.0006031

    Figure Lengend Snippet: In vivo survival and proliferation of expanded T regs and T effs (independently injected into the same mouse) indicating impaired survival of T eff-conditioned is not intrinsic to all transferred T effs , but instead due to co-culture with T regs . A) CFSE + and SNARF-1 cells recovered four days following independent injections of expanded CFSE + CD4 + CD25 + cells (containing T regs and T eff-conditioned ) and expanded SNARF-1 + CD4 + CD25 − T effs (unconditioned T effs ) demonstrating approximately equal survival of both populations (∼0.4% of recovered lymphocytes each). The ratio of injected cells on Day 0 was 45% T regs , 10% T eff-conditioned , 45% unconditioned T effs . B) Histogram of recovered CFSE + and SNARF-1 + cells at day 4 demonstrate persistence of “unconditioned” T effs (SNARF-1 + cells) equivalent to survival of the CFSE + T reg /T eff-conditioned mix, a finding not observed when only T regs and T eff-conditioned are co-transferred (as shown in Figure 5 ). C–D) Foxp3 analysis of sorted cells demonstrating that surviving CFSE + cells are T regs (97% Foxp3 + ) and SNARF-1 + cells are “unconditioned” T effs (Foxp3 − ), indicating impaired survival and proliferation of transferred T eff-conditioned is a consequence of co-culture with T reg during exogenous expansion (characteristic findings from 5 mice).

    Article Snippet: The anti-CD25 mAb (R-PE-conjugated, 7D4) and anti-CTLA4 mAb (R-PE-conjugated, 1B8) were purchased from Southern Biotechnology Associates, Inc (Birmingham, AL).

    Techniques: In Vivo, Injection, Co-Culture Assay, Mouse Assay

    CD4 + CD25 + T reg function and phenotype is enhanced by exogenous expansion. A) Freshly isolated CD62L HI and CD62L LO fractions of CD4 + CD25 + T regs demonstrate equivalent suppression capacity in vitro as measured by mixed lymphocyte suppression assay (p≥0.25 for each cell ratio, 3–6 mice per group). The Suppression Index measures the capacity of T regs to suppress T resp proliferation (as measured by decreased tritium-labeled thymidine incorporation) at different T resp ∶T reg ratios divided by the maximal T resp proliferation in the absence of T regs B) Ex vivo expansion of CD4 + CD25 + T regs significantly enhances the ability of these cells to suppress proliferation of T resp cells derived from 29-week-old B/W mice (p≤0.04 for each cell ratio, 6 mice per group). C–D) Foxp3 + and CTLA-4 (surface and intracellular) expression in exogenously expanded T regs were significantly greater than in freshly isolated T regs as measured by mean-fluorescent intensity (p = 0.007, p

    Journal: PLoS ONE

    Article Title: Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells

    doi: 10.1371/journal.pone.0006031

    Figure Lengend Snippet: CD4 + CD25 + T reg function and phenotype is enhanced by exogenous expansion. A) Freshly isolated CD62L HI and CD62L LO fractions of CD4 + CD25 + T regs demonstrate equivalent suppression capacity in vitro as measured by mixed lymphocyte suppression assay (p≥0.25 for each cell ratio, 3–6 mice per group). The Suppression Index measures the capacity of T regs to suppress T resp proliferation (as measured by decreased tritium-labeled thymidine incorporation) at different T resp ∶T reg ratios divided by the maximal T resp proliferation in the absence of T regs B) Ex vivo expansion of CD4 + CD25 + T regs significantly enhances the ability of these cells to suppress proliferation of T resp cells derived from 29-week-old B/W mice (p≤0.04 for each cell ratio, 6 mice per group). C–D) Foxp3 + and CTLA-4 (surface and intracellular) expression in exogenously expanded T regs were significantly greater than in freshly isolated T regs as measured by mean-fluorescent intensity (p = 0.007, p

    Article Snippet: The anti-CD25 mAb (R-PE-conjugated, 7D4) and anti-CTLA4 mAb (R-PE-conjugated, 1B8) were purchased from Southern Biotechnology Associates, Inc (Birmingham, AL).

    Techniques: Isolation, In Vitro, Suppression Assay, Mouse Assay, Labeling, Ex Vivo, Derivative Assay, Expressing

    CD4 + Foxp3 − T eff-conditioned demonstrate impaired in vivo survival and proliferation following adoptive transfer. At each time point, an aliquot of CFSE labeled cells was fixed and stained with Pacific-Blue Foxp3 to permit measurement of the ratio T regs and T eff-conditioned . A) Baseline FITC-CFSE fluorescence of expanded CD4 + CD25 + lymphocytes just prior to adoptive transfer. Foxp3 stain demonstrates this exogenous expanded population containing 85% Foxp3 + T regs and 15% Foxp3 − T eff-conditioined (contaminating T eff cells contained within the expanded T regs population) at the time of transfer. B–C) Dot plot and histogram of the FITC-CFSE fluorescent intensity of both Foxp3 + T regs and Foxp3 − T eff-conditioned cells recovered from lymphatic and solid organ tissue 4 days after adoptive transfer. Panels B–C demonstrate a significantly reduced survival and proliferation of T eff-conditioned as compared to T regs such that T eff-conditioned comprise

    Journal: PLoS ONE

    Article Title: Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells

    doi: 10.1371/journal.pone.0006031

    Figure Lengend Snippet: CD4 + Foxp3 − T eff-conditioned demonstrate impaired in vivo survival and proliferation following adoptive transfer. At each time point, an aliquot of CFSE labeled cells was fixed and stained with Pacific-Blue Foxp3 to permit measurement of the ratio T regs and T eff-conditioned . A) Baseline FITC-CFSE fluorescence of expanded CD4 + CD25 + lymphocytes just prior to adoptive transfer. Foxp3 stain demonstrates this exogenous expanded population containing 85% Foxp3 + T regs and 15% Foxp3 − T eff-conditioined (contaminating T eff cells contained within the expanded T regs population) at the time of transfer. B–C) Dot plot and histogram of the FITC-CFSE fluorescent intensity of both Foxp3 + T regs and Foxp3 − T eff-conditioned cells recovered from lymphatic and solid organ tissue 4 days after adoptive transfer. Panels B–C demonstrate a significantly reduced survival and proliferation of T eff-conditioned as compared to T regs such that T eff-conditioned comprise

    Article Snippet: The anti-CD25 mAb (R-PE-conjugated, 7D4) and anti-CTLA4 mAb (R-PE-conjugated, 1B8) were purchased from Southern Biotechnology Associates, Inc (Birmingham, AL).

    Techniques: In Vivo, Adoptive Transfer Assay, Labeling, Staining, Fluorescence

    Inhibition of proteinuria in T reg treated mice. Mice receiving adoptively transferred T regs had significantly reduced progression to proteinuria ≥100 mg/dl over the 12 weeks following transfer as compared to either PBS or CD4 + CD25 − T cell control groups (p≤0.025 starting week 8, n = 22 for T reg and T eff groups, n = 21 for PBS group).

    Journal: PLoS ONE

    Article Title: Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells

    doi: 10.1371/journal.pone.0006031

    Figure Lengend Snippet: Inhibition of proteinuria in T reg treated mice. Mice receiving adoptively transferred T regs had significantly reduced progression to proteinuria ≥100 mg/dl over the 12 weeks following transfer as compared to either PBS or CD4 + CD25 − T cell control groups (p≤0.025 starting week 8, n = 22 for T reg and T eff groups, n = 21 for PBS group).

    Article Snippet: The anti-CD25 mAb (R-PE-conjugated, 7D4) and anti-CTLA4 mAb (R-PE-conjugated, 1B8) were purchased from Southern Biotechnology Associates, Inc (Birmingham, AL).

    Techniques: Inhibition, Mouse Assay

    Differential APM component expression in immature and mature IFN DC and IL-4 DC. (A) Permeabilized control T2 and Colo38 cells were incubated with TAP1- and TAP2-specific mAb and analyzed by flow cytometry as a control. The percentage of positive cells and MFI are reported in each panel. (B) Permeabilized immature and mature IFN DC (bold line histogram) and IL-4 DC (black filled histogram) were incubated with TAP1-specific mAb NOB1, TAP2-specific mAb NOB2, calreticulin-specific mAb TO-11, calnexin-specific mAb TO-5, and tapasin-specific mAb TO-3 and subsequently stained with FITC-conjugated goat anti-mouse IgG antibodies. Stained cells were analyzed by flow cytometry. Cells incubated only with FITC-labeled goat anti-mouse IgG antibodies (gray filled and broken line histogram) were used as a negative control. The percentage of positive cells and MFI are reported under each panel.

    Journal: European journal of immunology

    Article Title: Differential expression of constitutive and inducible proteasome subunits in human monocyte-derived DC differentiated in the presence of IFN-α or IL-4

    doi: 10.1002/eji.200738098

    Figure Lengend Snippet: Differential APM component expression in immature and mature IFN DC and IL-4 DC. (A) Permeabilized control T2 and Colo38 cells were incubated with TAP1- and TAP2-specific mAb and analyzed by flow cytometry as a control. The percentage of positive cells and MFI are reported in each panel. (B) Permeabilized immature and mature IFN DC (bold line histogram) and IL-4 DC (black filled histogram) were incubated with TAP1-specific mAb NOB1, TAP2-specific mAb NOB2, calreticulin-specific mAb TO-11, calnexin-specific mAb TO-5, and tapasin-specific mAb TO-3 and subsequently stained with FITC-conjugated goat anti-mouse IgG antibodies. Stained cells were analyzed by flow cytometry. Cells incubated only with FITC-labeled goat anti-mouse IgG antibodies (gray filled and broken line histogram) were used as a negative control. The percentage of positive cells and MFI are reported under each panel.

    Article Snippet: Purified mouse IgG2b isotype control and purified mouse IgG1 isotype control were purchased from Southern Biotech (Birmingham, AL, USA).

    Techniques: Expressing, Incubation, Flow Cytometry, Cytometry, Staining, Labeling, Negative Control