pe conjugated armenian hamster igg  (Santa Cruz Biotechnology)

Journal:

Article Title: Natural Killer Cell Triggered Vascular Transformation: Maternal Care Before Birth?

doi: 10.1038/cmi.2010.38

Figure Lengend Snippet: Fluorescence photomicrographs showing EGFL7 expression at gd6 (a), gd8 (b), gd10 (c) and gd12 (d) in C57BL/6J implantation sites. PFA-fixed paraffin-embedded implantation sites were sectioned, stained with anti-EGFL7 (a kind gift from Dr Huilian Ye, Tumor Biology and Angiogenesis Department, Genentech Inc., South San Francisco, CA, USA), then visualized by PE-conjugated goat anti-Armenian Hamster IgG (red). DNA was labeled with DAPI (blue). EGFL7 had a restricted pattern of expression at the maternal–fetal interface. EGFL7 was transiently expressed at gd10 DB in vein ECs and downregulated by gd12. No uNK cell with detectable EGFL7 was seen. Modified spiral arteries that become vein-like EPHB4+ vessels are shown in gd10–12 implantation sites (e, f). UNK cells express the arterial marker EFNB2 at gd6 and 8 but lose this marker and become EPHB4+ (arrow; f) prior to the switch in expression of these molecules by the spiral arteries.24 DAPI, 4,6-diamidino-2-phenylindole; EGFL7, epidermal growth factor-like domain 7; gd, gestation day; IgG, immunoglobulin G; PE, phycoerythrin; PFA, paraformaldehyde; uNK cell, uterine natural killer cell.

Article Snippet: PFA-fixed paraffin-embedded implantation sites were sectioned, stained with anti-EGFL7 (a kind gift from Dr Huilian Ye, Tumor Biology and Angiogenesis Department, Genentech Inc., South San Francisco, CA, USA), then visualized by PE-conjugated goat anti-Armenian Hamster IgG (red).

Techniques: Fluorescence, Expressing, Staining, Labeling, Modification, Marker