pe conjugated anti mouse cd127  (BioLegend)

 
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  • 94
    Name:
    PE anti mouse CD127 IL 7Rα
    Description:
    PE anti mouse CD127 IL 7Rα SB 199 Isotype Rat IgG2b κ Reactivity Mouse Apps FC Size 50 μg
    Catalog Number:
    121111
    Price:
    110
    Category:
    Mouse Immunology
    Source:
    Rat
    Applications:
    FC
    Conjugate:
    PE
    Immunogen:
    mouse pre-B cell line 1A9
    Size:
    50 μg
    Quantity:
    1
    Preparation:
    The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions The solution is free of unconjugated PE and unconjugated antibody
    Buy from Supplier


    Structured Review

    BioLegend pe conjugated anti mouse cd127
    PE anti mouse CD127 IL 7Rα
    PE anti mouse CD127 IL 7Rα SB 199 Isotype Rat IgG2b κ Reactivity Mouse Apps FC Size 50 μg
    https://www.bioz.com/result/pe conjugated anti mouse cd127/product/BioLegend
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated anti mouse cd127 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Adjustments of γδ T Cells in the Lung of Schistosoma japonicum-Infected C56BL/6 Mice"

    Article Title: Adjustments of γδ T Cells in the Lung of Schistosoma japonicum-Infected C56BL/6 Mice

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.01045

    Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, CD127, CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, ** P
    Figure Legend Snippet: Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, CD127, CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, ** P

    Techniques Used: Expressing, Infection, Mouse Assay, Staining, FACS

    Related Articles

    Purification:

    Article Title: HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis.
    Article Snippet: .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-mouse Lineage Cocktail BioLegend Cat# 133303 Biotin anti-mouse Lineage Panel BioLegend Cat# 133307 PE-Cy7-Sca-1 BD Biosciences Cat# 558162 PerCP-Sca-1 BD Biosciences Cat# 108122 APC-c-Kit BioLegend Cat# 105812 APC-Fluor 780-c-Kit eBioscience Cat# 47-1171-82 FITC-CD48 eBioscience Cat# 11-0481-82 PerCP/Cy5.5-CD48 BioLegend Cat# 103422 APC-CD150 BioLegend Cat# 115909 Alexa Fluor 647-CD34 BD Biosciences Cat# 560230 FITC-CD16/32 BD Biosciences Cat# 553144 Purified anti-mouse CD16/32 Antibody BioLegend Cat# 101302 PE-CD127 BioLegend Cat# 135009 PerCP/Cy5.5-CD41 BioLegend Cat# 133917 Brilliant Violet 605-Ki67 BioLegend Cat# 652413 Alexa Fluor 488-H2A.X BioLegend Cat# 613405 FITC-CD43 BioLegend Cat# 143203 PerCP-B220 BioLegend Cat# 103234 APC-IgM BioLegend Cat# 406509 PE-IgD BioLegend Cat# 405705 PE-CD4 BioLegend Cat# 100408 PerCP-CD8 BioLegend Cat# 100732 APC-CD25 BioLegend Cat# 101909 FITC-CD44 BioLegend Cat# 103006 PerCP/Cy5.5-CD11b BioLegend Cat# 101228 FITC-Ly-6G/Ly-6C (Gr-1) BioLegend Cat# 108406 APC-Ly-6G BioLegend Cat# 127614 PE-Ly-6C BioLegend Cat# 128007 APC-F4/80 BioLegend Cat# 123116 PE-CD11c BioLegend Cat# 117308 APC/Cy7-CD3 BioLegend Cat# 100222 PE/Cy7-CD19 BioLegend Cat# 115519 FITC-CD5 BioLegend Cat# 100605 APC-Ter119 BioLegend Cat# 116212 PE-CD71 BioLegend Cat# 113808 APC-CD45.1 BioLegend Cat# 110714 PE-CD45.2 BioLegend Cat# 109808 Hira Peter Adams lab N/A TFIIB Abcam ab109518 Chemicals, Peptides, and Recombinant Proteins rh TPO STEMCELL Technologies Cat# 02522 rh IL11 R systems Cat# 218-IL-005 rh IL-6 Pepro Technology Cat# 200-06 (Continued on next page) e1 Cell Reports 30, 2136–2149.e1–e4, February 18, 2020. ..

    Recombinant:

    Article Title: HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis.
    Article Snippet: .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-mouse Lineage Cocktail BioLegend Cat# 133303 Biotin anti-mouse Lineage Panel BioLegend Cat# 133307 PE-Cy7-Sca-1 BD Biosciences Cat# 558162 PerCP-Sca-1 BD Biosciences Cat# 108122 APC-c-Kit BioLegend Cat# 105812 APC-Fluor 780-c-Kit eBioscience Cat# 47-1171-82 FITC-CD48 eBioscience Cat# 11-0481-82 PerCP/Cy5.5-CD48 BioLegend Cat# 103422 APC-CD150 BioLegend Cat# 115909 Alexa Fluor 647-CD34 BD Biosciences Cat# 560230 FITC-CD16/32 BD Biosciences Cat# 553144 Purified anti-mouse CD16/32 Antibody BioLegend Cat# 101302 PE-CD127 BioLegend Cat# 135009 PerCP/Cy5.5-CD41 BioLegend Cat# 133917 Brilliant Violet 605-Ki67 BioLegend Cat# 652413 Alexa Fluor 488-H2A.X BioLegend Cat# 613405 FITC-CD43 BioLegend Cat# 143203 PerCP-B220 BioLegend Cat# 103234 APC-IgM BioLegend Cat# 406509 PE-IgD BioLegend Cat# 405705 PE-CD4 BioLegend Cat# 100408 PerCP-CD8 BioLegend Cat# 100732 APC-CD25 BioLegend Cat# 101909 FITC-CD44 BioLegend Cat# 103006 PerCP/Cy5.5-CD11b BioLegend Cat# 101228 FITC-Ly-6G/Ly-6C (Gr-1) BioLegend Cat# 108406 APC-Ly-6G BioLegend Cat# 127614 PE-Ly-6C BioLegend Cat# 128007 APC-F4/80 BioLegend Cat# 123116 PE-CD11c BioLegend Cat# 117308 APC/Cy7-CD3 BioLegend Cat# 100222 PE/Cy7-CD19 BioLegend Cat# 115519 FITC-CD5 BioLegend Cat# 100605 APC-Ter119 BioLegend Cat# 116212 PE-CD71 BioLegend Cat# 113808 APC-CD45.1 BioLegend Cat# 110714 PE-CD45.2 BioLegend Cat# 109808 Hira Peter Adams lab N/A TFIIB Abcam ab109518 Chemicals, Peptides, and Recombinant Proteins rh TPO STEMCELL Technologies Cat# 02522 rh IL11 R systems Cat# 218-IL-005 rh IL-6 Pepro Technology Cat# 200-06 (Continued on next page) e1 Cell Reports 30, 2136–2149.e1–e4, February 18, 2020. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis.
    Article Snippet: .. KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE anti-mouse Lineage Cocktail BioLegend Cat# 133303 Biotin anti-mouse Lineage Panel BioLegend Cat# 133307 PE-Cy7-Sca-1 BD Biosciences Cat# 558162 PerCP-Sca-1 BD Biosciences Cat# 108122 APC-c-Kit BioLegend Cat# 105812 APC-Fluor 780-c-Kit eBioscience Cat# 47-1171-82 FITC-CD48 eBioscience Cat# 11-0481-82 PerCP/Cy5.5-CD48 BioLegend Cat# 103422 APC-CD150 BioLegend Cat# 115909 Alexa Fluor 647-CD34 BD Biosciences Cat# 560230 FITC-CD16/32 BD Biosciences Cat# 553144 Purified anti-mouse CD16/32 Antibody BioLegend Cat# 101302 PE-CD127 BioLegend Cat# 135009 PerCP/Cy5.5-CD41 BioLegend Cat# 133917 Brilliant Violet 605-Ki67 BioLegend Cat# 652413 Alexa Fluor 488-H2A.X BioLegend Cat# 613405 FITC-CD43 BioLegend Cat# 143203 PerCP-B220 BioLegend Cat# 103234 APC-IgM BioLegend Cat# 406509 PE-IgD BioLegend Cat# 405705 PE-CD4 BioLegend Cat# 100408 PerCP-CD8 BioLegend Cat# 100732 APC-CD25 BioLegend Cat# 101909 FITC-CD44 BioLegend Cat# 103006 PerCP/Cy5.5-CD11b BioLegend Cat# 101228 FITC-Ly-6G/Ly-6C (Gr-1) BioLegend Cat# 108406 APC-Ly-6G BioLegend Cat# 127614 PE-Ly-6C BioLegend Cat# 128007 APC-F4/80 BioLegend Cat# 123116 PE-CD11c BioLegend Cat# 117308 APC/Cy7-CD3 BioLegend Cat# 100222 PE/Cy7-CD19 BioLegend Cat# 115519 FITC-CD5 BioLegend Cat# 100605 APC-Ter119 BioLegend Cat# 116212 PE-CD71 BioLegend Cat# 113808 APC-CD45.1 BioLegend Cat# 110714 PE-CD45.2 BioLegend Cat# 109808 Hira Peter Adams lab N/A TFIIB Abcam ab109518 Chemicals, Peptides, and Recombinant Proteins rh TPO STEMCELL Technologies Cat# 02522 rh IL11 R systems Cat# 218-IL-005 rh IL-6 Pepro Technology Cat# 200-06 (Continued on next page) e1 Cell Reports 30, 2136–2149.e1–e4, February 18, 2020. ..

    Flow Cytometry:

    Article Title: MicroRNA-146a limits tumorigenic inflammation in colorectal cancer
    Article Snippet: .. The following antibodies were used for flow cytometry: anti-CD3-FITC (clone 17A2, Biolegend, Cat#100204, dilution 1 : 100); anti-CD4-APC (clone GK1.5, Biolegend, Cat#100412, dilution 1 : 100); anti-CD4-PE (clone GK1.5 Biolegend, Cat#100408, dilution 1 : 100); anti-CD4-Brilliant Violet 421 (clone GK1.5, Biolegend, Cat#100443, dilution 1 : 100); anti-IL-17A-PE (clone TC11-18H10.1, Biolegend, Cat#506904, dilution 1 : 100); anti-IL-17A-APC (clone TC11-18H10.1, Biolegend, Cat#506916, dilution 1 : 100); anti-CD11c-APC (clone N418, Biolegend, Cat#117310, dilution 1 : 100), anti-CD11c-PE (clone N418, Biolegend, Cat#117308, dilution 1 : 100); anti-CD11b-APC (clone M1/70, Biolegend, Cat#101212, dilution 1 : 100); anti-F4/80-PE/Cyanine 7 (clone BM8, Biolegend, Cat#123114, dilution 1 : 100), anti-I-A/I-E-PE (clone M5/114.15.2, Biolegend, Cat#107608, dilution 1 : 100); anti-TCR γ/δ-APC (clone GL3, Biolegend, Cat#118116, dilution 1 : 100); anti-CD326(Ep-CAM)-APC (clone G8.8, Biolegend, Cat#118214, dilution 1 : 100); anti-CD326(Ep-CAM)-PerCP/Cyanine 5.5 (clone G8.8, Biolegend, Cat#118220, dilution 1 : 100); anti-CD326 (Ep-CAM)-PE (clone VU1D9, Cell Signaling, Cat#8995, dilution 1 : 100); anti-CD45-FITC (clone 30-F11, Biolegend, Cat#103108, dilution 1 : 100); anti-CD45-APC (clone 30-F11, Biolegend, Cat#103112, dilution 1 : 100); anti-CD45-PerCP (clone 30-F11, Biolegend, Cat#103130, dilution 1 : 100); anti-CD45.1-PE/Cyanine 7 (clone A20, Biolegend, Cat#110730, dilution 1 : 100); anti-CD45.2-APC (clone 104, Biolegend, Cat#109814, dilution 1 : 100); anti-Lineage Cocktail-Brilliant Violet 421 (clone 17A2, Biolegend, Cat#133311, dilution 1 : 100); anti-CD127-PE (clone A7R34, Biolegend, Cat#135010, dilution 1 : 100); anti-p38 MAPK-PE (clone 36/p38 (pT180/pY182), BD Biosciences, Cat#612565, dilution 1 : 6); anti-TRAF6-PE (clone 210412, Abcam, Cat#ab210412, dilution 1 : 100); anti-Phospho-NF-κB-p65 (clone 93H1, Cell Signaling, Cat#5733, dilution 1 : 100); and anti-CD31-PerCP/Cyanine 5.5 (clone 390, Biolegend, Cat#102420, dilution 1 : 100). ..

    Staining:

    Article Title: Serum free culture for the expansion and study of type 2 innate lymphoid cells
    Article Snippet: .. Prior to sorting, the cells were stained with FITC-conjugated lineage marker mouse antibodies purchased from Invitrogen (CD3ε/γ #11-0032-80, CD4 #11-042-81, CD8α #11-0081-81, CD19 #11-0193-81, TCRβ #11-5961-81, NK1.1 #11-5941-81, TER119 #11-5921-81, CD11c #11-0114-81, CD11b #11-0112-41 and Ly-6G/C #11-9668-80) and the ILC2 markers purchased from BioLegend: PE-conjugated CD127 (IL-7 receptor, #135009), PerCP-Cy5.5-conjugated ST2 (IL-33 receptor #145312), BV605-conjugated Thy1.2 (#140317) and BV421-conjugated CD45 (#103133). ..

    Article Title: NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells
    Article Snippet: .. Measurement of apoptosis in Ova-CD8+ MPECs and Ova-EM Splenocytes were harvested from WT and T/NIX−/− mice 20 days after immunization with 104 PFU of VSV-Ova, pre-treated with FcRγII/III (Fc blocker) and IgG2b anti-mouse CD16/CD32 antibodies, and stained with the following anti-mouse fluorescent-conjugated antibodies: CD8 (Biolegend#100725), CD44 (Biolegend#103062), CD62L (Biolegend#104418), CD43 (Biolegend#121224), CD127 (Biolegend#135010) and SIINFEKL peptide-specific Ova_tetramer (Baylor College of Medicine’s MHC tetramer production facility#16114). ..

    Marker:

    Article Title: Serum free culture for the expansion and study of type 2 innate lymphoid cells
    Article Snippet: .. Prior to sorting, the cells were stained with FITC-conjugated lineage marker mouse antibodies purchased from Invitrogen (CD3ε/γ #11-0032-80, CD4 #11-042-81, CD8α #11-0081-81, CD19 #11-0193-81, TCRβ #11-5961-81, NK1.1 #11-5941-81, TER119 #11-5921-81, CD11c #11-0114-81, CD11b #11-0112-41 and Ly-6G/C #11-9668-80) and the ILC2 markers purchased from BioLegend: PE-conjugated CD127 (IL-7 receptor, #135009), PerCP-Cy5.5-conjugated ST2 (IL-33 receptor #145312), BV605-conjugated Thy1.2 (#140317) and BV421-conjugated CD45 (#103133). ..

    other:

    Article Title: Adjustments of γδ T Cells in the Lung of Schistosoma japonicum-Infected C56BL/6 Mice
    Article Snippet: PE-conjugated anti-mouse CD3 (145-2C11), Brilliant Violet 510-conjugated anti-mouse γδ TCR (GL3), APC-conjugated anti-mouse CD4 (RM4-5), PE-cy5-conjugated anti-mouse CD19 (6D5), PE-conjugated anti-mouse Vγ2 (A7R34), APC-conjugated anti-mouse CD69 (H1.2F3), PE-conjugated anti-mouse CD127 (A7R34), APC-conjugated anti-mouse CD62L (MEL-14), FITC-conjugated anti-mouse CD27 (LG.3A10), APC-conjugated anti-mouse IgD (11-26c.2a), FITC-conjugated anti-mouse MHCII (M5/114.15.2), PE-conjugated anti-mouse CD80 (16-10A1), PE-cy7-conjugated anti-mouse ICOS (C398.4A), APC-conjugated anti-mouse CX3CR1 (SA011F11), APC-conjugated anti-mouse IFN-γ (XMG1.2), Alexa Fluor 647-conjugated anti-mouse IL-21 (BL25168), PerCP-cy5.5-conjugated anti-mouse GM-CSF (MP1-22E9), PE-conjugated anti-mouse IL-1 (ALF-161), and APC-conjugated anti-mouse IL-6 (MP5-20F3) were purchased from BioLegend (San Diego, CA, USA).

    Mouse Assay:

    Article Title: NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells
    Article Snippet: .. Measurement of apoptosis in Ova-CD8+ MPECs and Ova-EM Splenocytes were harvested from WT and T/NIX−/− mice 20 days after immunization with 104 PFU of VSV-Ova, pre-treated with FcRγII/III (Fc blocker) and IgG2b anti-mouse CD16/CD32 antibodies, and stained with the following anti-mouse fluorescent-conjugated antibodies: CD8 (Biolegend#100725), CD44 (Biolegend#103062), CD62L (Biolegend#104418), CD43 (Biolegend#121224), CD127 (Biolegend#135010) and SIINFEKL peptide-specific Ova_tetramer (Baylor College of Medicine’s MHC tetramer production facility#16114). ..

    Isolation:

    Article Title: Developmental Stage-Specific Changes in Protein Synthesis Differentially Sensitize Hematopoietic Stem Cells and Erythroid Progenitors to Impaired Ribosome Biogenesis
    Article Snippet: .. For flow cytometric analysis and isolation, cells were incubated with combinations of antibodies (eBiosciences or BioLegend) to the following cell-surface markers, conjugated to FITC, PE, PerCP-Cy5.5, APC, PE-Cy7, Alexa Fluor 700, or APC-eFluor 780: CD3ε (17A2; Cat #100204), CD4 (GK1.5; Cat #100406), CD5 (53–7.3; Cat #100606), CD8α (53–6.7), CD11b (M1/70; Cat #12-0112-83 and 17-0112-82), CD45.1 (A20; Cat #47-0453-82), CD45.2 (104; Cat #109806), CD45R (RA3-6B2; Cat #11-0452-85 and #45-0452-82), CD48 (HM48-1; Cat #103404), CD71 (R17217; Cat #11-0711-85), CD117 (2B8; Cat #17-1171-83), CD127 (A7R34; Cat# 135010), CD150 (TC15-12F12.2; Cat #115904 and #115910), TER119 (TER-119; Cat# 116212), Sca-1 (D7, E13–161.7; Cat# 45-5,981-82), GR1 (RB6-8C5; Cat #108406 and #108416) and IgM (II/41; 17-5,790-82). ..

    Incubation:

    Article Title: Developmental Stage-Specific Changes in Protein Synthesis Differentially Sensitize Hematopoietic Stem Cells and Erythroid Progenitors to Impaired Ribosome Biogenesis
    Article Snippet: .. For flow cytometric analysis and isolation, cells were incubated with combinations of antibodies (eBiosciences or BioLegend) to the following cell-surface markers, conjugated to FITC, PE, PerCP-Cy5.5, APC, PE-Cy7, Alexa Fluor 700, or APC-eFluor 780: CD3ε (17A2; Cat #100204), CD4 (GK1.5; Cat #100406), CD5 (53–7.3; Cat #100606), CD8α (53–6.7), CD11b (M1/70; Cat #12-0112-83 and 17-0112-82), CD45.1 (A20; Cat #47-0453-82), CD45.2 (104; Cat #109806), CD45R (RA3-6B2; Cat #11-0452-85 and #45-0452-82), CD48 (HM48-1; Cat #103404), CD71 (R17217; Cat #11-0711-85), CD117 (2B8; Cat #17-1171-83), CD127 (A7R34; Cat# 135010), CD150 (TC15-12F12.2; Cat #115904 and #115910), TER119 (TER-119; Cat# 116212), Sca-1 (D7, E13–161.7; Cat# 45-5,981-82), GR1 (RB6-8C5; Cat #108406 and #108416) and IgM (II/41; 17-5,790-82). ..

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  • 94
    BioLegend pe conjugated anti mouse cd127
    Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, <t>CD127,</t> CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, ** P
    Pe Conjugated Anti Mouse Cd127, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated anti mouse cd127/product/BioLegend
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated anti mouse cd127 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    99
    BioLegend anti cd127 pe
    GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface <t>CD127</t> at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.
    Anti Cd127 Pe, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd127 pe/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd127 pe - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    99
    BioLegend pe anti mouse cd127
    IL-23 promotes the differentiation of ILC1 to ILC3 in the tumor microenvironment. (a) 1 × 10 6 hepa1–6-vector cells in 2 ml PBS were injected into C57BL/6 mice hydrodynamically. 4 days later, ILC1 cells [CD45 + lin(CD3,CD19,CD11b,NK1.1) − CD117 − NKp46 − T-bet + ] were detected as shown in the flow plots. (b–c) 1 × 10 6 hepa1–6-IL-23, hepa1–6-vector cells in 2 ml PBS or 2 ml PBS were injected into C57BL/6 mice hydrodynamically (n = 3 mice/group). The percent of ILC1 cells in CD45 + lin − population in the liver was analyzed on day 4 post injection. (d) <t>CD127</t> + ILC1 cells were sorted from hepa1–6 tumor-bearing mice and cultured in the presence of IL-12, IL-1β, IL-23, or IL-1β + IL-23 with IL-2 for 5 days. Cellular yield was shown at the end of the culture. (e) Flow cytometry analysis of the expression of IL-17, IFN-γ, RORγt, NKp46, and CD117 with or without various treatments was shown as dot plots or histograms (left panel) or as the percent of total live cells (right panel), respectively. (f) IFN-γ and (g) IL-17 production in the culture supernatants was measured by ELISA. (h) Experimental sketch of ILC1 adoptive transfer experiments. ILC1 cells were sorted from CD45.1 + C57BL/6 tumor-bearing mice and adoptively transferred into CD45.2 + C57BL/6 mice implanted with hepa1–6-IL-23 or hepa1–6-vector cells. 5 days later, the phenotypes of CD45.1 + cells in the liver were analyzed. (i) The percent of T-bet − RORγt + , T-bet + RORγt + , T-bet + RORγt − cells in CD45.1 + cells. Data are shown as mean ± SEM. One-way ANOVA followed by Tukey's multiple comparisons test (c,d,e,f,g) or Two-tail unpaired student's t-test (i) was used for statistical analysis. * p
    Pe Anti Mouse Cd127, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe anti mouse cd127/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe anti mouse cd127 - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

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    Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, CD127, CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, ** P

    Journal: Frontiers in Immunology

    Article Title: Adjustments of γδ T Cells in the Lung of Schistosoma japonicum-Infected C56BL/6 Mice

    doi: 10.3389/fimmu.2020.01045

    Figure Lengend Snippet: Surface molecule expression on pulmonary γδ T cells. Single lung cell suspensions were separated from naïve and infected mice, and cells were stained with monoclonal antibodies against mice: CD3, γδTCR, CD4, CD8, Vγ2, CD25, CD69, CD127, CD62L, MHC II, CD80, PDL1, PDL2, CXCR3, CXCR4, CXCR6, and CX3CR1. (A) Representative FACS analysis of three independent experiments is shown. (B) Percentages of surface molecules calculated from FACS analysis, a representative experiment from five independent experiments was shown. The error bars are SD, ** P

    Article Snippet: PE-conjugated anti-mouse CD3 (145-2C11), Brilliant Violet 510-conjugated anti-mouse γδ TCR (GL3), APC-conjugated anti-mouse CD4 (RM4-5), PE-cy5-conjugated anti-mouse CD19 (6D5), PE-conjugated anti-mouse Vγ2 (A7R34), APC-conjugated anti-mouse CD69 (H1.2F3), PE-conjugated anti-mouse CD127 (A7R34), APC-conjugated anti-mouse CD62L (MEL-14), FITC-conjugated anti-mouse CD27 (LG.3A10), APC-conjugated anti-mouse IgD (11-26c.2a), FITC-conjugated anti-mouse MHCII (M5/114.15.2), PE-conjugated anti-mouse CD80 (16-10A1), PE-cy7-conjugated anti-mouse ICOS (C398.4A), APC-conjugated anti-mouse CX3CR1 (SA011F11), APC-conjugated anti-mouse IFN-γ (XMG1.2), Alexa Fluor 647-conjugated anti-mouse IL-21 (BL25168), PerCP-cy5.5-conjugated anti-mouse GM-CSF (MP1-22E9), PE-conjugated anti-mouse IL-1 (ALF-161), and APC-conjugated anti-mouse IL-6 (MP5-20F3) were purchased from BioLegend (San Diego, CA, USA).

    Techniques: Expressing, Infection, Mouse Assay, Staining, FACS

    Surface marker expression by K b /gB 496 tetramer-positive CD8 + T cells after prime-boost ivag immunization with HPV-gBsec. Representative plots show the expression of CD103, CXCR3, CD62L, CD127, CD69, CD44, KLRG-1, and CD49a by K b /gB 496 tetramer-positive

    Journal: Journal of Virology

    Article Title: Topical Herpes Simplex Virus 2 (HSV-2) Vaccination with Human Papillomavirus Vectors Expressing gB/gD Ectodomains Induces Genital-Tissue-Resident Memory CD8+ T Cells and Reduces Genital Disease and Viral Shedding after HSV-2 Challenge

    doi: 10.1128/JVI.02380-14

    Figure Lengend Snippet: Surface marker expression by K b /gB 496 tetramer-positive CD8 + T cells after prime-boost ivag immunization with HPV-gBsec. Representative plots show the expression of CD103, CXCR3, CD62L, CD127, CD69, CD44, KLRG-1, and CD49a by K b /gB 496 tetramer-positive

    Article Snippet: After FcR blocking, cell suspensions were stained for 30 min at 4°C with an allophycocyanin (APC)-conjugated H-2Kb /gB496–503 tetramer (NIH Tetramer Facility) and the following antibodies: Pacific Blue-conjugated anti-CD3 (clone 17A2), APC- and Cy7-conjugated anti-CD4 (clone RM4-5), phycoerythrin (PE)- and Cy7-conjugated anti-CD44 (clone IM7), PE-conjugated anti-CD49a (clone HMα1), PE- and Cy7-conjugated anti-CD69 (clone H1.2F3), fluorescein isothiocyanate (FITC)-conjugated anti-CD62L (clone MEL-14), peridinin chlorophyll protein (PerCP)- and Cy5.5-conjugated anti-CD103 (clone 2E7), PE-conjugated anti-CD127 (clone SB/199), PE- and Cy7-conjugated anti-CXCR3 (clone CXCR3-173) (BioLegend), FITC-conjugated anti-KLRG-1 (clone 2F1; SouthernBiotech), and Pacific Orange-conjugated anti-CD8 (clone 53.6.7; Invitrogen).

    Techniques: Marker, Expressing

    GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.

    Journal: Frontiers in Immunology

    Article Title: Transient Expression of GATA3 in Hematopoietic Stem Cells Facilitates Helper Innate Lymphoid Cell Differentiation

    doi: 10.3389/fimmu.2019.00510

    Figure Lengend Snippet: GATA3 induces the development of ILC progenitors. Five-day expanded UCB CD34 + α4β7 − HSCs were transfected with either GATA3 mRNA (GATA3) or scrambled mRNA (control) and differentiated under conditions that favor the development of ILCs (A) Gating strategy, 5-days expanded CD34 + α4β7 − HSCs were sorted by FACS. (B) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of α4β7 + cells. (C,D) CD34 + α4β7 − HSCs were stained for the surface α4β7 at day 7 post electroporation and the percentage (C) and MFI (D) of α4β7 + cells is shown ( n = 4/group). (E) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD127 + cells. (F) CD34 + α4β7 − HSCs were stained for the surface CD127 at day 14 post electroporation and the percentage of CD127 + cells is shown in bar graph ( n = 4/group). (G) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and dot plot is shown ( n = 4). Values represent the percentage of CD117 + CD11a − ILCPs. (H) CD34 + α4β7 − HSCs were stained for the surface CD11a and CD117 at day 14 post electroporation and the percentage of CD117 + CD11a − ILCPs is shown in bar graph ( n = 4/group). (I) Total counts of transfected and differentiating HSCs were performed at day 14 of culture and live cells in 1 mL of culture media is depicted ( n = 4/group). (J) Absolute counts of CD117 + CD11a − ILCPs in 1 mL of culture media is depicted ( n = 4/group). (C,D,F,H–J) , Data are shown as means ± SD, and p -values are depicted and paired two-tailed t -tests.

    Article Snippet: Expression of surface receptor was determined using the following monoclonal antibodies: anti-CD3-PerCp5.5 (clone HIT3a), anti-CD11c-PerCp5.5 (clone Bu15), anti-D14-PerCp5.5 (clone 63D3), anti-CD19-PerCp5.5 (clone HIB19), anti-CD123-PerCp5.5 (clone 6H6), anti-CD127-PE (clone A019D5), anti-CD294-BV421/BV605 (clone BM16), anti-CD336-APC (clone P44-8), and anti-mouse IgG-FITC/PE (clone Poly4053) (all from Biolegend); anti-CD11a-FITC (clone G43-25B), anti-CD34-PE/APC (clone 4H11), anti-CD45-APC/PE (clone HI30), anti-CD56-BV421/BV605 (clone NCAM16.2), anti-CD94-PerCp5.5/FITC (clone HP-3D9), anti-CD117-PE-Cy7 (clone 104D2), and anti-CD161-PE (clone DX12) (all from BD Biosciences); and anti-α4β7 (Cat#11718, from NIH).

    Techniques: Transfection, FACS, Staining, Electroporation, Two Tailed Test

    IL-23 promotes the differentiation of ILC1 to ILC3 in the tumor microenvironment. (a) 1 × 10 6 hepa1–6-vector cells in 2 ml PBS were injected into C57BL/6 mice hydrodynamically. 4 days later, ILC1 cells [CD45 + lin(CD3,CD19,CD11b,NK1.1) − CD117 − NKp46 − T-bet + ] were detected as shown in the flow plots. (b–c) 1 × 10 6 hepa1–6-IL-23, hepa1–6-vector cells in 2 ml PBS or 2 ml PBS were injected into C57BL/6 mice hydrodynamically (n = 3 mice/group). The percent of ILC1 cells in CD45 + lin − population in the liver was analyzed on day 4 post injection. (d) CD127 + ILC1 cells were sorted from hepa1–6 tumor-bearing mice and cultured in the presence of IL-12, IL-1β, IL-23, or IL-1β + IL-23 with IL-2 for 5 days. Cellular yield was shown at the end of the culture. (e) Flow cytometry analysis of the expression of IL-17, IFN-γ, RORγt, NKp46, and CD117 with or without various treatments was shown as dot plots or histograms (left panel) or as the percent of total live cells (right panel), respectively. (f) IFN-γ and (g) IL-17 production in the culture supernatants was measured by ELISA. (h) Experimental sketch of ILC1 adoptive transfer experiments. ILC1 cells were sorted from CD45.1 + C57BL/6 tumor-bearing mice and adoptively transferred into CD45.2 + C57BL/6 mice implanted with hepa1–6-IL-23 or hepa1–6-vector cells. 5 days later, the phenotypes of CD45.1 + cells in the liver were analyzed. (i) The percent of T-bet − RORγt + , T-bet + RORγt + , T-bet + RORγt − cells in CD45.1 + cells. Data are shown as mean ± SEM. One-way ANOVA followed by Tukey's multiple comparisons test (c,d,e,f,g) or Two-tail unpaired student's t-test (i) was used for statistical analysis. * p

    Journal: EBioMedicine

    Article Title: NCR− group 3 innate lymphoid cells orchestrate IL-23/IL-17 axis to promote hepatocellular carcinoma development

    doi: 10.1016/j.ebiom.2019.02.050

    Figure Lengend Snippet: IL-23 promotes the differentiation of ILC1 to ILC3 in the tumor microenvironment. (a) 1 × 10 6 hepa1–6-vector cells in 2 ml PBS were injected into C57BL/6 mice hydrodynamically. 4 days later, ILC1 cells [CD45 + lin(CD3,CD19,CD11b,NK1.1) − CD117 − NKp46 − T-bet + ] were detected as shown in the flow plots. (b–c) 1 × 10 6 hepa1–6-IL-23, hepa1–6-vector cells in 2 ml PBS or 2 ml PBS were injected into C57BL/6 mice hydrodynamically (n = 3 mice/group). The percent of ILC1 cells in CD45 + lin − population in the liver was analyzed on day 4 post injection. (d) CD127 + ILC1 cells were sorted from hepa1–6 tumor-bearing mice and cultured in the presence of IL-12, IL-1β, IL-23, or IL-1β + IL-23 with IL-2 for 5 days. Cellular yield was shown at the end of the culture. (e) Flow cytometry analysis of the expression of IL-17, IFN-γ, RORγt, NKp46, and CD117 with or without various treatments was shown as dot plots or histograms (left panel) or as the percent of total live cells (right panel), respectively. (f) IFN-γ and (g) IL-17 production in the culture supernatants was measured by ELISA. (h) Experimental sketch of ILC1 adoptive transfer experiments. ILC1 cells were sorted from CD45.1 + C57BL/6 tumor-bearing mice and adoptively transferred into CD45.2 + C57BL/6 mice implanted with hepa1–6-IL-23 or hepa1–6-vector cells. 5 days later, the phenotypes of CD45.1 + cells in the liver were analyzed. (i) The percent of T-bet − RORγt + , T-bet + RORγt + , T-bet + RORγt − cells in CD45.1 + cells. Data are shown as mean ± SEM. One-way ANOVA followed by Tukey's multiple comparisons test (c,d,e,f,g) or Two-tail unpaired student's t-test (i) was used for statistical analysis. * p

    Article Snippet: The FITC-anti-mouse-CD8 (53–6.7), FITC-anti-mouse-CD4 (GK1.5), PE-anti-mouse-IL-22 (Poly5.64), PE-anti-mouse-CD127 (SB/199), PE-anti-mouse-IL-17A (TC11-18H10.1), PE-anti-mouse-IFN-γ (XMG1.2), PE-anti-mouse-I-Ab (AF6–120.1), PE-anti-mouse-H-2Kb (AF6–88.5), PE-Rat-IgG2b, kappa Isotype (RTK4530), PerCP/Cy5.5-anti-mouse-TCRγδ (GL3), PerCP/Cy5.5-anti-mouse-IL-17A (TC11-18H10.1), PerCP/Cy5.5-anti-mouse-CD45.1 (A20), APC-anti-mouse-CD8 (53–6.7), APC-anti-mouse-CD117 (ACK2), Alexa fluor 700-anti-mouse-CD45 (30-F11), APC-anti-mouse-IFN-γ (XMG1.2), PE/Cy7-anti-mouse-TNF-α (MP6-XT22), and PE/Cy7-anti-mouse-T-bet (4B10), APC/Cy7-anti-mouse-CD127 (A7R34) were purchased from BioLegend (San Diego, CA).

    Techniques: Plasmid Preparation, Injection, Mouse Assay, Flow Cytometry, Cell Culture, Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Adoptive Transfer Assay