pe conjugated anti human cd4  (BioLegend)

 
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    Name:
    PE anti human CD4
    Description:
    PE anti human CD4 RPA T4 Isotype Mouse IgG1 κ Reactivity Human Cross Reactivity Chimpanzee Apps FC Size 25 tests
    Catalog Number:
    300507
    Price:
    25
    Applications:
    FC
    Conjugate:
    PE
    Size:
    25 tests
    Category:
    Human Immunology
    Preparation:
    The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions The solution is free of unconjugated PE and unconjugated antibody
    Source:
    Mouse
    Quantity:
    1
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    Structured Review

    BioLegend pe conjugated anti human cd4
    PE anti human CD4
    PE anti human CD4 RPA T4 Isotype Mouse IgG1 κ Reactivity Human Cross Reactivity Chimpanzee Apps FC Size 25 tests
    https://www.bioz.com/result/pe conjugated anti human cd4/product/BioLegend
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pe conjugated anti human cd4 - by Bioz Stars, 2020-07
    97/100 stars

    Images

    1) Product Images from "Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist"

    Article Title: Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist

    Journal: Nature Communications

    doi: 10.1038/s41467-019-12901-3

    Increase in polyfunctional T and NK cells in PBMC of patients receiving NKTR-214. Single-cell polyfunctional strength index (PSI) computed for CD4, CD8 T cells and NK cells across 5 patients at different time-points. C1D1: cycle 1 day 1; C1D8: cycle 1 day 8; C2D1: cycle 2 day 1; C2D8: cycle 2 day 8; C3D1: cycle 3 day 1.
    Figure Legend Snippet: Increase in polyfunctional T and NK cells in PBMC of patients receiving NKTR-214. Single-cell polyfunctional strength index (PSI) computed for CD4, CD8 T cells and NK cells across 5 patients at different time-points. C1D1: cycle 1 day 1; C1D8: cycle 1 day 8; C2D1: cycle 2 day 1; C2D8: cycle 2 day 8; C3D1: cycle 3 day 1.

    Techniques Used:

    2) Product Images from "Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients"

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients

    Journal: Cellular and Molecular Immunology

    doi: 10.1038/cmi.2018.5

    Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P
    Figure Legend Snippet: Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P

    Techniques Used: Expressing, In Vitro, Fluorescence, Staining, MANN-WHITNEY, Two Tailed Test

    M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.
    Figure Legend Snippet: M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.

    Techniques Used: Activation Assay, Expressing

    3) Product Images from "Up-regulation of gap junction in peripheral blood T lymphocytes contributes to the inflammatory response in essential hypertension"

    Article Title: Up-regulation of gap junction in peripheral blood T lymphocytes contributes to the inflammatory response in essential hypertension

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0184773

    EH induced changes in subtypes of peripheral blood T lymphocytes. A, Representative flow cytometry analysis showing expression levels of circulating subtypes of T lymphocytes in the peripheral blood of 20 EHs and 20 age- and sex-matched NTs. Fresh, resting PBMCs from EHs and NTs were stained with antibodies against CD3, CD4, and CD8 molecules and analyzed by flow cytometry. Based on the CD3 + gate, cells were further gated based on CD4 or CD8 expression and the frequency of CD4 + or CD8 + T cells was determined. B, Frequency of CD3 + , CD4 + , and CD8 + T cells in the peripheral blood of EHs and NTs. Fresh, resting PBMCs from EHs and NTs were stained with anti-CD3, anti-CD4, and anti-CD8 and then the cells were gated for CD3 positive cells, CD3 and CD4 or CD8 double positive cells after gating for lymphocytes by forward and side scatters. The vertical axis represents the frequency of various T lymphocyte subtypes. Quantitative analysis of the mean percentage of cells ± SEM. * p
    Figure Legend Snippet: EH induced changes in subtypes of peripheral blood T lymphocytes. A, Representative flow cytometry analysis showing expression levels of circulating subtypes of T lymphocytes in the peripheral blood of 20 EHs and 20 age- and sex-matched NTs. Fresh, resting PBMCs from EHs and NTs were stained with antibodies against CD3, CD4, and CD8 molecules and analyzed by flow cytometry. Based on the CD3 + gate, cells were further gated based on CD4 or CD8 expression and the frequency of CD4 + or CD8 + T cells was determined. B, Frequency of CD3 + , CD4 + , and CD8 + T cells in the peripheral blood of EHs and NTs. Fresh, resting PBMCs from EHs and NTs were stained with anti-CD3, anti-CD4, and anti-CD8 and then the cells were gated for CD3 positive cells, CD3 and CD4 or CD8 double positive cells after gating for lymphocytes by forward and side scatters. The vertical axis represents the frequency of various T lymphocyte subtypes. Quantitative analysis of the mean percentage of cells ± SEM. * p

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Staining

    The effect of EH on the expression and production of pro-inflammatory cytokines. A, Quantitative analysis of intracellular staining of pro-inflammatory cytokine (IFN-γ or TNF-α) expression levels in CD4 + and CD8 + T lymphocyte population of EHs, as determined by fluorescence activated cell sorting analysis. PBMCs from EHs and NTs underwent surface staining with antibodies against CD3, CD4, and CD8 molecules. After surface staining, the cells were fixed and permeabilized and stained with FITC-conjugated anti-human IFN-γ and Alexa Flour 488-conjugated anti-human TNF-ɑ. Based on the CD4 + or CD8 + gate, cells were further gated based on IFN-γ and TNF-ɑ expression levels, and the percentage of CD4 + or CD8 + T cells producing IFN-γ and TNF-ɑ was determined. The results shown are the values ± SEM of the percentage IFN-γ- or TNF-α-producing CD4 + or CD8 + T cells; * p
    Figure Legend Snippet: The effect of EH on the expression and production of pro-inflammatory cytokines. A, Quantitative analysis of intracellular staining of pro-inflammatory cytokine (IFN-γ or TNF-α) expression levels in CD4 + and CD8 + T lymphocyte population of EHs, as determined by fluorescence activated cell sorting analysis. PBMCs from EHs and NTs underwent surface staining with antibodies against CD3, CD4, and CD8 molecules. After surface staining, the cells were fixed and permeabilized and stained with FITC-conjugated anti-human IFN-γ and Alexa Flour 488-conjugated anti-human TNF-ɑ. Based on the CD4 + or CD8 + gate, cells were further gated based on IFN-γ and TNF-ɑ expression levels, and the percentage of CD4 + or CD8 + T cells producing IFN-γ and TNF-ɑ was determined. The results shown are the values ± SEM of the percentage IFN-γ- or TNF-α-producing CD4 + or CD8 + T cells; * p

    Techniques Used: Expressing, Staining, Fluorescence, FACS

    Gap27 alters pro-inflammatory cytokines production in T lymphocytes from EHs and NTs. Representative dot plots of T lymphocytes from unstimulated PBMCs of EHs and NTs. PBMCs of EHs and NTs were stimulated in vitro with IL-2 (1000 U/ml) for 24 h. Gap27 (500 μM) was added to culture supernatants for the last 48 h. Subsequently, the cells were harvested and stained for CD4 + /CD8 + , IFN-γ and TNF-α for flow cytometry analysis (A and D). B and C, The percentage of CD4 + (B) or CD8 + (C) T cells expressing IFN-γ are presented as the percentages of total CD3 + T cells. E and F, Percentage of CD4 + or CD8 + T cells among CD3 + T cells expressing TNF-α. Data show the mean ± SEM of the percentage of IFNγ or TNF-α producing CD4 + or CD8 + T cells. ** p
    Figure Legend Snippet: Gap27 alters pro-inflammatory cytokines production in T lymphocytes from EHs and NTs. Representative dot plots of T lymphocytes from unstimulated PBMCs of EHs and NTs. PBMCs of EHs and NTs were stimulated in vitro with IL-2 (1000 U/ml) for 24 h. Gap27 (500 μM) was added to culture supernatants for the last 48 h. Subsequently, the cells were harvested and stained for CD4 + /CD8 + , IFN-γ and TNF-α for flow cytometry analysis (A and D). B and C, The percentage of CD4 + (B) or CD8 + (C) T cells expressing IFN-γ are presented as the percentages of total CD3 + T cells. E and F, Percentage of CD4 + or CD8 + T cells among CD3 + T cells expressing TNF-α. Data show the mean ± SEM of the percentage of IFNγ or TNF-α producing CD4 + or CD8 + T cells. ** p

    Techniques Used: In Vitro, Staining, Flow Cytometry, Cytometry, Expressing

    Increased expression of Cxs in CD4 + and CD8 + T lymphocytes from EHs. A, Representative flow cytometry plots are presented for Cx40 and Cx43 expression levels on gated single-positive CD4 + T lymphocytes or CD8 + T lymphocyte populations in the peripheral blood from 20 EHs and 20 age- and sex-matched NTs . Fresh, resting PBMCs from EHs and NTs underwent surface staining with antibodies against CD3, CD4, and CD8 molecules. After surface staining, the cells were fixed, permeabilized and stained with unlabeled anti–Cx40 or anti–Cx43 plus FITC-labelledsecondary antibodies. Based on the CD4 + or CD8 + gate, the cells were further gated based on Cx40 and Cx43 expression levels, and the frequency of CD4 + or CD8 + T cells expressing Cx40 and Cx43 was determined. B, The percentage of CD4 + or CD8 + T cell population is presented for Cx40 and Cx43. Both Cx40 and Cx43 expression levels are significantly increased in CD4 + or CD8 + T cells of patients with hypertension compared with those of NTs. Values are mean ± SEM. ** p
    Figure Legend Snippet: Increased expression of Cxs in CD4 + and CD8 + T lymphocytes from EHs. A, Representative flow cytometry plots are presented for Cx40 and Cx43 expression levels on gated single-positive CD4 + T lymphocytes or CD8 + T lymphocyte populations in the peripheral blood from 20 EHs and 20 age- and sex-matched NTs . Fresh, resting PBMCs from EHs and NTs underwent surface staining with antibodies against CD3, CD4, and CD8 molecules. After surface staining, the cells were fixed, permeabilized and stained with unlabeled anti–Cx40 or anti–Cx43 plus FITC-labelledsecondary antibodies. Based on the CD4 + or CD8 + gate, the cells were further gated based on Cx40 and Cx43 expression levels, and the frequency of CD4 + or CD8 + T cells expressing Cx40 and Cx43 was determined. B, The percentage of CD4 + or CD8 + T cell population is presented for Cx40 and Cx43. Both Cx40 and Cx43 expression levels are significantly increased in CD4 + or CD8 + T cells of patients with hypertension compared with those of NTs. Values are mean ± SEM. ** p

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Staining

    Related Articles

    Flow Cytometry:

    Article Title: Characterization of Human CD39+ Th17 Cells with Suppressor Activity and Modulation in Inflammatory Bowel Disease
    Article Snippet: .. Flow Cytometry Cell phenotype was assessed by 6-colour flow cytometry following cell incubation with FITC, PE, PE-Cy7, Pacific blue (PB), APC and APC-Cy7-conjugated anti-human antibodies to: CD4 (clone#: OKT4), CD45RO (clone#: UCHL1), CD45RA (clone#: HI100), CD25 (clone#: BC96), CD26 (clone#: BA5b), CD39 (clone#: A1), CD73 (clone#: AD2), CCR6 (clone#: G034E3) (all from Biolegend, San Diego, CA) and IL-23R (R & D Systems, clone#: 218213). .. Frequency of FOXP3, RORC and Stat-3 positive cells was assessed by intracellular staining following cell fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and incubation with PB, APC and PE-conjugated anti-human FOXP3 (Biolegend, clone#: 206D), RORC (eBioscience, San Diego, CA; clone#: AFKJS-9) and Stat-3 (BD Bioscience, clone #: 49/p-Stat-3).

    Article Title: HIV-antibody complexes enhance production of type I interferon by plasmacytoid dendritic cells
    Article Snippet: .. After sorting, pDC purity was determined by flow cytometry using known pDC markers PE-Cy7 mouse anti-CD123 (BD Biosciences, catalog 560826), BV421 mouse anti-CD303, and PE mouse anti-CD4 (BioLegend, catalog 5354212 and 344606). .. The pDC cultures were 95% pure based on CD303, CD123, and CD4 expression.

    Article Title: Umbilical Cord-derived Mesenchymal Stem Cells Instruct Monocytes Towards an IL10-producing Phenotype by Secreting IL6 and HGF
    Article Snippet: .. Flow cytometry The antibodies used for flow cytometry were FITC-, PE- or APC-conjugated mouse anti-human CD3, CD4, HLA-DR, CD1a, CD14, CD80, CD86, and CD83, all of which were purchased from BioLegend. .. The cultured cells were collected, washed twice, and resuspended in 100 μl of PBS containing 0.1% BSA.

    Cytometry:

    Article Title: Characterization of Human CD39+ Th17 Cells with Suppressor Activity and Modulation in Inflammatory Bowel Disease
    Article Snippet: .. Flow Cytometry Cell phenotype was assessed by 6-colour flow cytometry following cell incubation with FITC, PE, PE-Cy7, Pacific blue (PB), APC and APC-Cy7-conjugated anti-human antibodies to: CD4 (clone#: OKT4), CD45RO (clone#: UCHL1), CD45RA (clone#: HI100), CD25 (clone#: BC96), CD26 (clone#: BA5b), CD39 (clone#: A1), CD73 (clone#: AD2), CCR6 (clone#: G034E3) (all from Biolegend, San Diego, CA) and IL-23R (R & D Systems, clone#: 218213). .. Frequency of FOXP3, RORC and Stat-3 positive cells was assessed by intracellular staining following cell fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and incubation with PB, APC and PE-conjugated anti-human FOXP3 (Biolegend, clone#: 206D), RORC (eBioscience, San Diego, CA; clone#: AFKJS-9) and Stat-3 (BD Bioscience, clone #: 49/p-Stat-3).

    Article Title: HIV-antibody complexes enhance production of type I interferon by plasmacytoid dendritic cells
    Article Snippet: .. After sorting, pDC purity was determined by flow cytometry using known pDC markers PE-Cy7 mouse anti-CD123 (BD Biosciences, catalog 560826), BV421 mouse anti-CD303, and PE mouse anti-CD4 (BioLegend, catalog 5354212 and 344606). .. The pDC cultures were 95% pure based on CD303, CD123, and CD4 expression.

    Article Title: Umbilical Cord-derived Mesenchymal Stem Cells Instruct Monocytes Towards an IL10-producing Phenotype by Secreting IL6 and HGF
    Article Snippet: .. Flow cytometry The antibodies used for flow cytometry were FITC-, PE- or APC-conjugated mouse anti-human CD3, CD4, HLA-DR, CD1a, CD14, CD80, CD86, and CD83, all of which were purchased from BioLegend. .. The cultured cells were collected, washed twice, and resuspended in 100 μl of PBS containing 0.1% BSA.

    Incubation:

    Article Title: Characterization of Human CD39+ Th17 Cells with Suppressor Activity and Modulation in Inflammatory Bowel Disease
    Article Snippet: .. Flow Cytometry Cell phenotype was assessed by 6-colour flow cytometry following cell incubation with FITC, PE, PE-Cy7, Pacific blue (PB), APC and APC-Cy7-conjugated anti-human antibodies to: CD4 (clone#: OKT4), CD45RO (clone#: UCHL1), CD45RA (clone#: HI100), CD25 (clone#: BC96), CD26 (clone#: BA5b), CD39 (clone#: A1), CD73 (clone#: AD2), CCR6 (clone#: G034E3) (all from Biolegend, San Diego, CA) and IL-23R (R & D Systems, clone#: 218213). .. Frequency of FOXP3, RORC and Stat-3 positive cells was assessed by intracellular staining following cell fixation and permeabilization with Cytofix/Cytoperm (BD Biosciences) and incubation with PB, APC and PE-conjugated anti-human FOXP3 (Biolegend, clone#: 206D), RORC (eBioscience, San Diego, CA; clone#: AFKJS-9) and Stat-3 (BD Bioscience, clone #: 49/p-Stat-3).

    Staining:

    Article Title: Reiterative Enrichment and Authentication of CRISPRi Targets (REACT) identifies the proteasome as a key contributor to HIV-1 latency
    Article Snippet: .. The cells were stained with LIVE/DEAD Cell Stain Kit (Invitrogen, L34955) and PE-conjugated anti-human CD4 Antibody (BioLegend, 317410). ..

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients
    Article Snippet: .. Whole blood was stained with PE-conjugated anti-human CD4 (RPTA-4; BioLegend) and FITC-conjugated anti-human CD45RA (clone HI100; BioLegend) for 30 min on ice in the dark. .. After incubation, red blood cell lysis was performed (Red Blood Cell lysis buffer, Roche, Basel, Switzerland) according to the manufacturer’s instructions, followed by washing with PBS supplemented with 10% FCS (Sigma Aldrich, St Louis, MO, USA).

    Article Title: Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist
    Article Snippet: .. Enriched CD4 and CD8 T cells were resuspended in fresh complete RPMI media at 1 × 106 /ml and activated with immobilized anti-human CD3 (10 ug/ml, Thermo Fisher Scientific) and soluble anti-human CD28 (5 ug/ml, Thermo Fisher Scientific) in a 96-well flat-bottom plate (Corning Life Science) at 37 °C, 5% CO2 for 24 h. After stimulation, cells were stained with PE-conjugated anti-human CD4 (Biolegend) or Alexa Fluor 647-conjugated anti-human CD8 (Biolegend) at room temperature for 20 min and loaded onto an IsoCode chip. .. Each IsoCode chip contains ~12,000 microchambers pre-patterned with a full copy of 32-plex antibody array including Effector: Granzyme B, TNFα, IFN-γ, MIP1α, Perforin, TNFβ; Stimulatory: GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-9, IL-12, IL-15, IL-21; Chemoattractive: CCL11, IP-10, MIP-1β, RANTES; Regulatory: IL-4, IL-10, IL-13, IL-22, sCD137, sCD40L, TGFβ1; Inflammatory: IL-6, IL-17A, IL-17F, MCP-1, MCP-4, IL-1β.

    Recombinase Polymerase Amplification:

    Article Title: A Natural Killer/Dendritic Cell Axis Defines Responsive Tumor Microenvironments in Melanoma
    Article Snippet: .. CD45 clone Hl30 (eBioscience 47–0459-42), CD3e clone OKT3 (eBioscience 46–0037-42), HLA-DR clone L243 (eBioscience 48–9952-42), CD56 clone CMSSB (eBioscience 46–0567-42), CD56 clone HCD65 (BioLegend 318304), CD19 clone H1B19 (eBioscience 46–0198-42 and 56–0199-42), CD14 clone 61D3 (Invitrogen ; Cohort 1), CD14 clone M5E2 (BioLegend 301836; Cohort 2), CD16 clone 3G8 (BioLegend 302040), CD11c clone 3.9 (eBioscience 56–0116-42), CD85g clone 17G10.2 (eBioscience 12–5179-42), BDCA1 clone L161 (BioLegend 331516), BDCA3 clone AD5–14H12 (Miltenyi 130–090-513), CD4 clone RPA-T4 (BioLegend 300550), CD8 clone RPA-T8 (BioLegend 301040), CD25 clone BC96 (BioLegend 302612), CD25 clone 2A3 (eBioscience 17–0259-42), FoxP3 clone PCH101 (eBioscience 77–5776-40), FoxP3 clone 236A/E7 (eBioscience 25–4777-42), PD-1 clone EH-12 (BioLegend 329930), CTLA-4 clone BNI3 (BioLegend 369606), γδ TCR clone B1.1 (BioLegend 331212), Flt3L (Abcam ab9688). .. All antibodies were purchased from BD Pharmingen, eBioscience, Invitrogen, BioLegend, the UCSF hybridoma core, R & D Systems, Abcam, or were produced in the Krummel Lab.

    Chromatin Immunoprecipitation:

    Article Title: Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist
    Article Snippet: .. Enriched CD4 and CD8 T cells were resuspended in fresh complete RPMI media at 1 × 106 /ml and activated with immobilized anti-human CD3 (10 ug/ml, Thermo Fisher Scientific) and soluble anti-human CD28 (5 ug/ml, Thermo Fisher Scientific) in a 96-well flat-bottom plate (Corning Life Science) at 37 °C, 5% CO2 for 24 h. After stimulation, cells were stained with PE-conjugated anti-human CD4 (Biolegend) or Alexa Fluor 647-conjugated anti-human CD8 (Biolegend) at room temperature for 20 min and loaded onto an IsoCode chip. .. Each IsoCode chip contains ~12,000 microchambers pre-patterned with a full copy of 32-plex antibody array including Effector: Granzyme B, TNFα, IFN-γ, MIP1α, Perforin, TNFβ; Stimulatory: GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-9, IL-12, IL-15, IL-21; Chemoattractive: CCL11, IP-10, MIP-1β, RANTES; Regulatory: IL-4, IL-10, IL-13, IL-22, sCD137, sCD40L, TGFβ1; Inflammatory: IL-6, IL-17A, IL-17F, MCP-1, MCP-4, IL-1β.

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    BioLegend cd4
    Expansion of multicytokine-producing vaccine-specific <t>CD4</t> + T cells. ( A ) Percentage of cytokine-producing vaccine-specific CD4 + T cells before immunization and 28 d postboost in TIV- (blue) and ATIV- (red) vaccinated individuals, respectively. Significant
    Cd4, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 1178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4/product/BioLegend
    Average 97 stars, based on 1178 article reviews
    Price from $9.99 to $1999.99
    cd4 - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    91
    BioLegend pe conjugated mouse anti human cd4 mab
    Antagonistic effect of compounds on CXCL12-induced calcium signaling in <t>U87.CD4.CXCR4</t> cells. Fluo-2 AM loaded cells were treated with serial dilutions of compound and subsequently stimulated with 6.25 nM CXCL12. Fluorescence changes were measured over time in all wells simultaneously. (A): The inhibitory effect of different concentrations of AMD11070 on CXCL12-induced calcium release is shown. The red and blue line represent the negative (untreated, without CXCL12 stimulation) and positive (untreated, CXCL12 stimulation) control, respectively. Calcium fluxes are represented as % of baseline ( i . e . mean relative light units in each well during a fixed time interval before CXCL12 addition). One representative experiment out of three is shown with each data point corresponding to the mean fluorescence of three to six replicates. (B), (C) and (D): Inhibitory effect of (B) T22, T140, TC14012, CTCE-9908, (C) AMD3100, AMD3465, AMD11070, IT1t, (D) WZ811, Me6TREN and gambogic acid on CXCL12-induced calcium flux. Data are represented as % inhibition of CXCL12-induced calcium mobilization relative to the positive and negative control. Mean ± SEM of three independent experiments is shown.
    Pe Conjugated Mouse Anti Human Cd4 Mab, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated mouse anti human cd4 mab/product/BioLegend
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated mouse anti human cd4 mab - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Expansion of multicytokine-producing vaccine-specific CD4 + T cells. ( A ) Percentage of cytokine-producing vaccine-specific CD4 + T cells before immunization and 28 d postboost in TIV- (blue) and ATIV- (red) vaccinated individuals, respectively. Significant

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Systems biology of immunity to MF59-adjuvanted versus nonadjuvanted trivalent seasonal influenza vaccines in early childhood

    doi: 10.1073/pnas.1519690113

    Figure Lengend Snippet: Expansion of multicytokine-producing vaccine-specific CD4 + T cells. ( A ) Percentage of cytokine-producing vaccine-specific CD4 + T cells before immunization and 28 d postboost in TIV- (blue) and ATIV- (red) vaccinated individuals, respectively. Significant

    Article Snippet: CD4+ T cells were stained with monoclonal antibodies against human CD3 (conjugated to APC-H7, clone SK7; BD Biosciences) and CD4 (conjugated to PerCP, clone RPA-T4; BioLegend).

    Techniques:

    Increase in polyfunctional T and NK cells in PBMC of patients receiving NKTR-214. Single-cell polyfunctional strength index (PSI) computed for CD4, CD8 T cells and NK cells across 5 patients at different time-points. C1D1: cycle 1 day 1; C1D8: cycle 1 day 8; C2D1: cycle 2 day 1; C2D8: cycle 2 day 8; C3D1: cycle 3 day 1.

    Journal: Nature Communications

    Article Title: Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist

    doi: 10.1038/s41467-019-12901-3

    Figure Lengend Snippet: Increase in polyfunctional T and NK cells in PBMC of patients receiving NKTR-214. Single-cell polyfunctional strength index (PSI) computed for CD4, CD8 T cells and NK cells across 5 patients at different time-points. C1D1: cycle 1 day 1; C1D8: cycle 1 day 8; C2D1: cycle 2 day 1; C2D8: cycle 2 day 8; C3D1: cycle 3 day 1.

    Article Snippet: Enriched CD4 and CD8 T cells were resuspended in fresh complete RPMI media at 1 × 106 /ml and activated with immobilized anti-human CD3 (10 ug/ml, Thermo Fisher Scientific) and soluble anti-human CD28 (5 ug/ml, Thermo Fisher Scientific) in a 96-well flat-bottom plate (Corning Life Science) at 37 °C, 5% CO2 for 24 h. After stimulation, cells were stained with PE-conjugated anti-human CD4 (Biolegend) or Alexa Fluor 647-conjugated anti-human CD8 (Biolegend) at room temperature for 20 min and loaded onto an IsoCode chip.

    Techniques:

    Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P

    Journal: Cellular and Molecular Immunology

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients

    doi: 10.1038/cmi.2018.5

    Figure Lengend Snippet: Constitutive and IL-6-induced STAT3 phosphorylation and SOCS3 expression in CD4 + T-cells from tuberculosis patients and healthy contacts. ( a – c ) Phosphorylation of STAT3 with or without IL-6 in vitro stimulation and ( d ) SOCS3 expression of CD4 + T-cells from tuberculosis patients and healthy contacts. ( a ) Representative histograms indicating non-stimulated (w/o) and IL-6-induced pSTAT3 expression of samples from a tuberculosis patient (left graph) and a healthy contact (right graph) are shown. Dotted lines indicate similar mean fluorescence intensities (MFI) of non-stained control samples for both respective study groups. ( b ) Study group comparisons of non-stimulated (w/o) and IL-6-induced pSTAT3 levels are shown. ( c ) The ratios between non-stimulated pSTAT3 and pSTAT5 values calculated for each individual donor. ( d ) SOCS3 expression for all CD4 + T-cells (left graph) as well as for CD45RA high and CD45RA low (right graph) CD4 + T-cell subpopulations are shown. Tuberculosis patients are represented by grey squares, healthy contacts are depicted as open circles. Study group medians and percentiles (25, 75) are given. Significant differences are indicated by asterisks. Nominal P -values for the Mann-Whitney U -test (two-tailed) were calculated and shown as * for P

    Article Snippet: Whole blood was stained with PE-conjugated anti-human CD4 (RPTA-4; BioLegend) and FITC-conjugated anti-human CD45RA (clone HI100; BioLegend) for 30 min on ice in the dark.

    Techniques: Expressing, In Vitro, Fluorescence, Staining, MANN-WHITNEY, Two Tailed Test

    M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.

    Journal: Cellular and Molecular Immunology

    Article Title: Constitutive STAT3 phosphorylation and IL-6/IL-10 co-expression are associated with impaired T-cell function in tuberculosis patients

    doi: 10.1038/cmi.2018.5

    Figure Lengend Snippet: M. tuberculosis -specific activation and intracellular cytokine expression in association with SOCS3 expression in both study groups. ( a ) Representative graphs depicting the gating procedure for intracellular cytokine analysis are shown. ( b ) Proportions of CD40L/IL-2-positive CD4 + T-cells are shown for tuberculosis patients (grey squares) and healthy contacts (open circles). ( c ) Correlation plots between SOCS3 expression and CD40L/IL-2 (upper graph) as well as CD40L/IFN-γ (lower graph) are shown for CD4 + T-cells. The Spearman Rank test was used to determine significant correlations for all donors and both study groups separately. Correlation coefficients (rho) and nominal P -values are given. ns: not significant.

    Article Snippet: Whole blood was stained with PE-conjugated anti-human CD4 (RPTA-4; BioLegend) and FITC-conjugated anti-human CD45RA (clone HI100; BioLegend) for 30 min on ice in the dark.

    Techniques: Activation Assay, Expressing

    Antagonistic effect of compounds on CXCL12-induced calcium signaling in U87.CD4.CXCR4 cells. Fluo-2 AM loaded cells were treated with serial dilutions of compound and subsequently stimulated with 6.25 nM CXCL12. Fluorescence changes were measured over time in all wells simultaneously. (A): The inhibitory effect of different concentrations of AMD11070 on CXCL12-induced calcium release is shown. The red and blue line represent the negative (untreated, without CXCL12 stimulation) and positive (untreated, CXCL12 stimulation) control, respectively. Calcium fluxes are represented as % of baseline ( i . e . mean relative light units in each well during a fixed time interval before CXCL12 addition). One representative experiment out of three is shown with each data point corresponding to the mean fluorescence of three to six replicates. (B), (C) and (D): Inhibitory effect of (B) T22, T140, TC14012, CTCE-9908, (C) AMD3100, AMD3465, AMD11070, IT1t, (D) WZ811, Me6TREN and gambogic acid on CXCL12-induced calcium flux. Data are represented as % inhibition of CXCL12-induced calcium mobilization relative to the positive and negative control. Mean ± SEM of three independent experiments is shown.

    Journal: PLoS ONE

    Article Title: Comparison of cell-based assays for the identification and evaluation of competitive CXCR4 inhibitors

    doi: 10.1371/journal.pone.0176057

    Figure Lengend Snippet: Antagonistic effect of compounds on CXCL12-induced calcium signaling in U87.CD4.CXCR4 cells. Fluo-2 AM loaded cells were treated with serial dilutions of compound and subsequently stimulated with 6.25 nM CXCL12. Fluorescence changes were measured over time in all wells simultaneously. (A): The inhibitory effect of different concentrations of AMD11070 on CXCL12-induced calcium release is shown. The red and blue line represent the negative (untreated, without CXCL12 stimulation) and positive (untreated, CXCL12 stimulation) control, respectively. Calcium fluxes are represented as % of baseline ( i . e . mean relative light units in each well during a fixed time interval before CXCL12 addition). One representative experiment out of three is shown with each data point corresponding to the mean fluorescence of three to six replicates. (B), (C) and (D): Inhibitory effect of (B) T22, T140, TC14012, CTCE-9908, (C) AMD3100, AMD3465, AMD11070, IT1t, (D) WZ811, Me6TREN and gambogic acid on CXCL12-induced calcium flux. Data are represented as % inhibition of CXCL12-induced calcium mobilization relative to the positive and negative control. Mean ± SEM of three independent experiments is shown.

    Article Snippet: The antibodies used in this study were phycoerythrin- (PE) and allophycocyanin (APC)-labeled mouse anti-human CXCR4 monoclonal antibodies (mAb) (clone 12G5; BD Pharmingen, San Diego, CA, USA), PE-conjugated rat anti-human CXCR4 mAb (clone 1D9; BD Pharmingen), PE-labeled mouse anti-human CXC chemokine receptor 7 (CXCR7) mAb (clone 10D1-J16; BioLegend, San Diego, CA, USA) and PE-conjugated mouse anti-human CD4 mAb (clone SK3; Biolegend) with the corresponding isotype controls [PE-labeled mouse IgG2a, κ isotype control mAb (clone G155-178; BD Pharmingen), APC-labeled mouse IgG2a, κ isotype control mAb (clone G155-178; BD Pharmingen), PE-conjugated rat IgG2a, κ isotype control mAb (clone R35-95; BD Pharmingen) and PE-labeled mouse IgG1, κ isotype control mAb (clone MOPC-21; BD Pharmingen)].

    Techniques: Fluorescence, Inhibition, Negative Control