pe anti sox2  (BioLegend)

 
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    Name:
    PE anti SOX2
    Description:
    PE anti SOX2 14A6A34 Isotype Mouse IgG1 κ Reactivity Human Mouse Apps ICFC Size 25 tests
    Catalog Number:
    656103
    Price:
    125
    Applications:
    ICFC
    Conjugate:
    PE
    Immunogen:
    Full length human SOX recombinant protein
    Size:
    25 tests
    Category:
    Cell Biology
    Preparation:
    The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions The solution is free of unconjugated PE and unconjugated antibody
    Source:
    Mouse
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioLegend pe anti sox2
    PE anti SOX2
    PE anti SOX2 14A6A34 Isotype Mouse IgG1 κ Reactivity Human Mouse Apps ICFC Size 25 tests
    https://www.bioz.com/result/pe anti sox2/product/BioLegend
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe anti sox2 - by Bioz Stars, 2020-07
    93/100 stars

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    cd34
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    Images

    Related Articles

    Staining:

    Article Title: Inhibition of WNT signaling attenuates self-renewal of SHH-subgroup medulloblastoma
    Article Snippet: .. For analyses by flow cytometry, cell suspensions from SCs or tumor tissues were incubated in Cytofix/Cytoperm solution (Becton & Dickinson, Franklin Lakes, NJ, USA) prior to staining with the corresponding fluorescent-conjugated antibody: SOX2 #656103, PCNA #307909 (BioLegend, San Diego, CA, USA), and CLEAVED CASPASE-3 #9669 (Cell Signaling). .. Fluorescence activated cell sorting (FACS) data were acquired using a BD CANTO II FACS (Becton & Dickinson) and analyzed using DiVa6 software.

    Article Title: FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation
    Article Snippet: .. Antibody staining was performed in permeabilization buffer with the following antibodies: rat OCT¾-eFluor660 (eBioscience, 50–5841-82, 1:50), rat SOX2-Alexa Fluor 488 (eBioscience, 53–9811-82, 1:50), mouse SOX2-PE (Biolegend, 656103, 1:50), mouse anti-SOX17-PE (BD Biosciences, 561591, 1:50), goat anti-FOXA2-Alexa Fluor 488 (R & D, IC2400G, 1:50), mouse anti-HNF1B (Santa Cruz, sc-130407, 1:100), goat anti-PDX1 (R & D, AF2419, 1:250), mouse anti-NKX6–1 (DSHB, F55A12-c, 1:250) and Alexa Fluor secondary antibodies (Molecular Probes, 1:500). .. Flow cytometry data was analyzed by FlowJo V9.

    Incubation:

    Article Title: The MMP-1/PAR-1 Axis Enhances Proliferation and Neuronal Differentiation of Adult Hippocampal Neural Progenitor Cells
    Article Snippet: .. Cells were washed 3 times in PBS and incubated overnight in antibody solution (Phycoerythrin (PE) conjugated anti-SOX2; 656103, BioLegend, San Diego, CA) made up in PBS-TX at 4°C. ..

    Article Title: Inhibition of WNT signaling attenuates self-renewal of SHH-subgroup medulloblastoma
    Article Snippet: .. For analyses by flow cytometry, cell suspensions from SCs or tumor tissues were incubated in Cytofix/Cytoperm solution (Becton & Dickinson, Franklin Lakes, NJ, USA) prior to staining with the corresponding fluorescent-conjugated antibody: SOX2 #656103, PCNA #307909 (BioLegend, San Diego, CA, USA), and CLEAVED CASPASE-3 #9669 (Cell Signaling). .. Fluorescence activated cell sorting (FACS) data were acquired using a BD CANTO II FACS (Becton & Dickinson) and analyzed using DiVa6 software.

    Article Title: Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells
    Article Snippet: .. For flow cytometry, the cells (106 cells/100 μ l) were collected and incubated with monoclonal phycoerythrin (PE)-conjugated antibodies for CD29 (cat. no. 303003), CD31 (cat. no. 303105), CD34 (cat. no. 343505), CD44 (cat. no. 338807), CD45 (cat. no. 368509), CD49d (cat. no. 304303), CD73 (cat. no. 344003), CD90 (cat. no. 32810), CD105 (cat. no. 323205), HLA-DR (cat. no. 307605), SSEA-4 (cat. no. 330405), SOX-2 (cat. no. 656103) and OCT-4 (cat. no. 653703) (BioLegend, San Diego, CA, USA) for 30 min on ice. .. Appropriate isotype-matched antibodies were used as negative controls (BD Biosciences, San Jose, CA, USA).

    Flow Cytometry:

    Article Title: Association of elevated reactive oxygen species and hyperthermia induced radiosensitivity in cancer stem-like cells
    Article Snippet: .. The triple positive (CD44+ CD24+ ESA+ ) cells and L3.6pl were reanalyzed by flow cytometry analysis using PE-labeled Sox2 antibodies (Biolegend). .. In sorted cell implantation experiments, CSCs were isolated using APC-labeled antibody against human CD133 (Miltenyi Biotech).

    Article Title: Inhibition of WNT signaling attenuates self-renewal of SHH-subgroup medulloblastoma
    Article Snippet: .. For analyses by flow cytometry, cell suspensions from SCs or tumor tissues were incubated in Cytofix/Cytoperm solution (Becton & Dickinson, Franklin Lakes, NJ, USA) prior to staining with the corresponding fluorescent-conjugated antibody: SOX2 #656103, PCNA #307909 (BioLegend, San Diego, CA, USA), and CLEAVED CASPASE-3 #9669 (Cell Signaling). .. Fluorescence activated cell sorting (FACS) data were acquired using a BD CANTO II FACS (Becton & Dickinson) and analyzed using DiVa6 software.

    Article Title: Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells
    Article Snippet: .. For flow cytometry, the cells (106 cells/100 μ l) were collected and incubated with monoclonal phycoerythrin (PE)-conjugated antibodies for CD29 (cat. no. 303003), CD31 (cat. no. 303105), CD34 (cat. no. 343505), CD44 (cat. no. 338807), CD45 (cat. no. 368509), CD49d (cat. no. 304303), CD73 (cat. no. 344003), CD90 (cat. no. 32810), CD105 (cat. no. 323205), HLA-DR (cat. no. 307605), SSEA-4 (cat. no. 330405), SOX-2 (cat. no. 656103) and OCT-4 (cat. no. 653703) (BioLegend, San Diego, CA, USA) for 30 min on ice. .. Appropriate isotype-matched antibodies were used as negative controls (BD Biosciences, San Jose, CA, USA).

    Cytometry:

    Article Title: Association of elevated reactive oxygen species and hyperthermia induced radiosensitivity in cancer stem-like cells
    Article Snippet: .. The triple positive (CD44+ CD24+ ESA+ ) cells and L3.6pl were reanalyzed by flow cytometry analysis using PE-labeled Sox2 antibodies (Biolegend). .. In sorted cell implantation experiments, CSCs were isolated using APC-labeled antibody against human CD133 (Miltenyi Biotech).

    Article Title: Inhibition of WNT signaling attenuates self-renewal of SHH-subgroup medulloblastoma
    Article Snippet: .. For analyses by flow cytometry, cell suspensions from SCs or tumor tissues were incubated in Cytofix/Cytoperm solution (Becton & Dickinson, Franklin Lakes, NJ, USA) prior to staining with the corresponding fluorescent-conjugated antibody: SOX2 #656103, PCNA #307909 (BioLegend, San Diego, CA, USA), and CLEAVED CASPASE-3 #9669 (Cell Signaling). .. Fluorescence activated cell sorting (FACS) data were acquired using a BD CANTO II FACS (Becton & Dickinson) and analyzed using DiVa6 software.

    Article Title: Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells
    Article Snippet: .. For flow cytometry, the cells (106 cells/100 μ l) were collected and incubated with monoclonal phycoerythrin (PE)-conjugated antibodies for CD29 (cat. no. 303003), CD31 (cat. no. 303105), CD34 (cat. no. 343505), CD44 (cat. no. 338807), CD45 (cat. no. 368509), CD49d (cat. no. 304303), CD73 (cat. no. 344003), CD90 (cat. no. 32810), CD105 (cat. no. 323205), HLA-DR (cat. no. 307605), SSEA-4 (cat. no. 330405), SOX-2 (cat. no. 656103) and OCT-4 (cat. no. 653703) (BioLegend, San Diego, CA, USA) for 30 min on ice. .. Appropriate isotype-matched antibodies were used as negative controls (BD Biosciences, San Jose, CA, USA).

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  • 93
    BioLegend pcna
    The propagation and growth of Trp53 -deficient, SHH-subgroup medulloblastoma is WNT-dependent. Trp53 mutant, Ptch1 -driven MB tissue, which had never been cultured ex vivo , was orthotopically implanted into the cerebellum of immunocompromised mice. These mice were then treated with carnosate or vehicle for 20 days, or until they developed MB symptoms. The mice were then killed and their brains collected. ( a ) Representative hematoxylin eosin staining of orthotopic MB tissue from a carnosate or vehicle-treated mouse is shown. ( b ) The volume of residual orthotopic tumors from these mice was calculated, and the mean volume and s.e.m. shown. ( c ) The residual orthotopic tumors from these mice were immunostained for the proliferation biomarker <t>PCNA,</t> and the numbers of PCNA + cells quantified and normalized to total cell number per field (%). ( d ) The residual orthotopic tumors from these mice were immunostained for the MPC biomarker <t>SOX2,</t> and the numbers of SOX2 + cells quantified and normalized to total cell number per field (%). ( e ) MB tumor tissue was subcutaneously implanted into the flanks of immunocompromised mice, and treated daily with of carnosate, or vehicle control, for 8 days. The expression of the indicated genes in residual flank tumors was then determined. ( f ) The enrichment of SOX2 + cells in residual flank tissue, from carnosate or vehicle-treated mice, was determined by FACS analysis. ( g ) The indicated number of viable cells from residual MB tissue was transplanted into additional immunocompromised mice, and the frequency of tumor engraftment determined. ( h ) Equal numbers of viable cells from residual flank MB tissue were implanted into the cerebellum of additional immunocompromised mice, and MB symptom-free survival monitored for 60 days. Results were normalized to vehicle control.
    Pcna, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcna/product/BioLegend
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pcna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    The propagation and growth of Trp53 -deficient, SHH-subgroup medulloblastoma is WNT-dependent. Trp53 mutant, Ptch1 -driven MB tissue, which had never been cultured ex vivo , was orthotopically implanted into the cerebellum of immunocompromised mice. These mice were then treated with carnosate or vehicle for 20 days, or until they developed MB symptoms. The mice were then killed and their brains collected. ( a ) Representative hematoxylin eosin staining of orthotopic MB tissue from a carnosate or vehicle-treated mouse is shown. ( b ) The volume of residual orthotopic tumors from these mice was calculated, and the mean volume and s.e.m. shown. ( c ) The residual orthotopic tumors from these mice were immunostained for the proliferation biomarker PCNA, and the numbers of PCNA + cells quantified and normalized to total cell number per field (%). ( d ) The residual orthotopic tumors from these mice were immunostained for the MPC biomarker SOX2, and the numbers of SOX2 + cells quantified and normalized to total cell number per field (%). ( e ) MB tumor tissue was subcutaneously implanted into the flanks of immunocompromised mice, and treated daily with of carnosate, or vehicle control, for 8 days. The expression of the indicated genes in residual flank tumors was then determined. ( f ) The enrichment of SOX2 + cells in residual flank tissue, from carnosate or vehicle-treated mice, was determined by FACS analysis. ( g ) The indicated number of viable cells from residual MB tissue was transplanted into additional immunocompromised mice, and the frequency of tumor engraftment determined. ( h ) Equal numbers of viable cells from residual flank MB tissue were implanted into the cerebellum of additional immunocompromised mice, and MB symptom-free survival monitored for 60 days. Results were normalized to vehicle control.

    Journal: Oncogene

    Article Title: Inhibition of WNT signaling attenuates self-renewal of SHH-subgroup medulloblastoma

    doi: 10.1038/onc.2017.232

    Figure Lengend Snippet: The propagation and growth of Trp53 -deficient, SHH-subgroup medulloblastoma is WNT-dependent. Trp53 mutant, Ptch1 -driven MB tissue, which had never been cultured ex vivo , was orthotopically implanted into the cerebellum of immunocompromised mice. These mice were then treated with carnosate or vehicle for 20 days, or until they developed MB symptoms. The mice were then killed and their brains collected. ( a ) Representative hematoxylin eosin staining of orthotopic MB tissue from a carnosate or vehicle-treated mouse is shown. ( b ) The volume of residual orthotopic tumors from these mice was calculated, and the mean volume and s.e.m. shown. ( c ) The residual orthotopic tumors from these mice were immunostained for the proliferation biomarker PCNA, and the numbers of PCNA + cells quantified and normalized to total cell number per field (%). ( d ) The residual orthotopic tumors from these mice were immunostained for the MPC biomarker SOX2, and the numbers of SOX2 + cells quantified and normalized to total cell number per field (%). ( e ) MB tumor tissue was subcutaneously implanted into the flanks of immunocompromised mice, and treated daily with of carnosate, or vehicle control, for 8 days. The expression of the indicated genes in residual flank tumors was then determined. ( f ) The enrichment of SOX2 + cells in residual flank tissue, from carnosate or vehicle-treated mice, was determined by FACS analysis. ( g ) The indicated number of viable cells from residual MB tissue was transplanted into additional immunocompromised mice, and the frequency of tumor engraftment determined. ( h ) Equal numbers of viable cells from residual flank MB tissue were implanted into the cerebellum of additional immunocompromised mice, and MB symptom-free survival monitored for 60 days. Results were normalized to vehicle control.

    Article Snippet: For analyses by flow cytometry, cell suspensions from SCs or tumor tissues were incubated in Cytofix/Cytoperm solution (Becton & Dickinson, Franklin Lakes, NJ, USA) prior to staining with the corresponding fluorescent-conjugated antibody: SOX2 #656103, PCNA #307909 (BioLegend, San Diego, CA, USA), and CLEAVED CASPASE-3 #9669 (Cell Signaling).

    Techniques: Mutagenesis, Cell Culture, Ex Vivo, Mouse Assay, Staining, Biomarker Assay, Expressing, FACS

    Identification of sorted breast CSCs and pancreatic CSCs in vitro and in vivo ( A ) Typical mammosphere formation of CD44 + CD24 – cells and MCF7 cells imaged by 10x objective (Bar = 100 μm). ( B ) Quantification of mammospheres derived from CD44 + CD24 – cells and MCF7 cells at day 7 after sham-treated, HT (43°C for 2 hours) or 2 Gy treatment. ( C ) Intracellular ROS concentrations of CD44 + CD24 – cells and MCF7 cells. ( D ) Clonogenic survival assay in cells derived from CD44 + CD24 + ESA + cells (squares) and L3.6pl cells (circles). To determine surviving fractions, counts were normalized using the plating efficiency of the unirradiated corresponding control. The survival curves of CD44 + CD24 + ESA + cells (solid line) and L3.6pl cells (dashed line) were fitted using LQ model respectively. ( E ) FACS analysis to measure Sox2 expression of L3.6pl cells and CD44 + CD24 + ESA + cells. ( F ) Representative experiment depicting tumor formation in a mouse at the injection site of 500 CD133 + cells, with no tumor formation seen at the injection site of 500 CD133 - cells. The results are presented as the mean ± SD, as determined from three independent experiments. ** P

    Journal: Oncotarget

    Article Title: Association of elevated reactive oxygen species and hyperthermia induced radiosensitivity in cancer stem-like cells

    doi: 10.18632/oncotarget.21678

    Figure Lengend Snippet: Identification of sorted breast CSCs and pancreatic CSCs in vitro and in vivo ( A ) Typical mammosphere formation of CD44 + CD24 – cells and MCF7 cells imaged by 10x objective (Bar = 100 μm). ( B ) Quantification of mammospheres derived from CD44 + CD24 – cells and MCF7 cells at day 7 after sham-treated, HT (43°C for 2 hours) or 2 Gy treatment. ( C ) Intracellular ROS concentrations of CD44 + CD24 – cells and MCF7 cells. ( D ) Clonogenic survival assay in cells derived from CD44 + CD24 + ESA + cells (squares) and L3.6pl cells (circles). To determine surviving fractions, counts were normalized using the plating efficiency of the unirradiated corresponding control. The survival curves of CD44 + CD24 + ESA + cells (solid line) and L3.6pl cells (dashed line) were fitted using LQ model respectively. ( E ) FACS analysis to measure Sox2 expression of L3.6pl cells and CD44 + CD24 + ESA + cells. ( F ) Representative experiment depicting tumor formation in a mouse at the injection site of 500 CD133 + cells, with no tumor formation seen at the injection site of 500 CD133 - cells. The results are presented as the mean ± SD, as determined from three independent experiments. ** P

    Article Snippet: The triple positive (CD44+ CD24+ ESA+ ) cells and L3.6pl were reanalyzed by flow cytometry analysis using PE-labeled Sox2 antibodies (Biolegend).

    Techniques: In Vitro, In Vivo, Derivative Assay, Clonogenic Cell Survival Assay, FACS, Expressing, Injection