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Abcam pdh e1a
Pdh E1a, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pdh e1a/product/Abcam
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pdh e1a - by Bioz Stars, 2020-07
85/100 stars

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Article Title: Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiforme
Article Snippet: Membranes were probed overnight with the following antibodies: β-actin (1:20,000; Sigma-Aldrich); PDH-E1a (1:200; Abcam); VEGF (1:200; Santa Cruz Biotechnology, Inc.,); HIF1α (1:250; BD); and PKM2, HK2, HK1, and PHD2 (1:1,000; Cell Signaling Technology).

Article Title: Nicotinamide mononucleotide requires SIRT3 to improve cardiac function and bioenergetics in a Friedreich’s ataxia cardiomyopathy model
Article Snippet: Primary antibodies were purchased from the following companies: acetylated-lysine (Cell Signaling Technology [CST], 9441); SIRT3 (D22A3; CST, 5490); pAMPKa (Th172; CST, 2535P); AMPKa (CST, 2603P); total OXPHOS (MitoSciences, Abcam, ab110413); pPDH (S293; CST, Calbiochem, AP1062); PDH E1A (Abcam, 1101416); GLUT1 (CST, 12939); GLUT4 (Abcam, ab654-250); and GAPDH (CST, 97166).

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  • 95
    Abcam anti pdh e1a
    Cell-wide clearance of mitochondria via autophagy is triggered by depolarization and proceeds over a 24-h time period Representative immunoblot of whole-cell lysates from U2OS:GFP and GFP-parkin cells treated with DMSO, 25 μM antimycin A (anti A), 25 μM antimycin A with 10 μM oligomycin (anti A + oligo), or 20 μM CCCP for the indicated time period. Quantification of <t>PDH</t> <t>E1a</t> signal intensity relative to that of actin in immunoblots from (A). Error bars represent the mean ± s.e.m. ( n = 3 experiments). Quantification of mitochondrial clearance in U2OS:GFP-parkin cells treated as in (A), fixed and immunostained for TOM20. Data are shown as percentage of cells containing mitochondria, by TOM20 staining, visualized by fluorescence microscopy. Error bars represent the mean ± s.e.m. ( n = 3 experiments, with at least 85 cells quantified per condition, per experiment). U2OS:GFP-parkin cells were treated as in (A) for the indicated time period, then fixed and immunostained for TOM20 (red). Cell boundaries are delineated in single-channel images. Scale bars, 20 μm. Source data are available online for this figure.
    Anti Pdh E1a, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pdh e1a/product/Abcam
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti pdh e1a - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    95
    Abcam phospho pdh e1a
    A model of microbial regulation of glucose metabolism in host colonocytes. Microbes increase blood glucose levels and increase <t>PDH</t> levels and activity to facilitate oxidative metabolism of glucose in the mitochondria. In contrast, GF mice are hypoglycemic, and their colonocytes respond by increased expression of GLUTs. Although this leads to increased glucose uptake, Glu-6-P and pyruvate levels are diminished because of their conversion to lactate, which is increased in GF colonocytes. This increase in glycolysis can be attributed to decreased PDH expression/activity and decreased oxidative metabolism in the mitochondria. Not only are PDH levels decreased, but also enzymatic activity is inhibited via phosphorylation of the <t>E1a</t> regulatory subunit due to increased expression of PDK2 in GF colonocytes.
    Phospho Pdh E1a, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho pdh e1a/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho pdh e1a - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Cell-wide clearance of mitochondria via autophagy is triggered by depolarization and proceeds over a 24-h time period Representative immunoblot of whole-cell lysates from U2OS:GFP and GFP-parkin cells treated with DMSO, 25 μM antimycin A (anti A), 25 μM antimycin A with 10 μM oligomycin (anti A + oligo), or 20 μM CCCP for the indicated time period. Quantification of PDH E1a signal intensity relative to that of actin in immunoblots from (A). Error bars represent the mean ± s.e.m. ( n = 3 experiments). Quantification of mitochondrial clearance in U2OS:GFP-parkin cells treated as in (A), fixed and immunostained for TOM20. Data are shown as percentage of cells containing mitochondria, by TOM20 staining, visualized by fluorescence microscopy. Error bars represent the mean ± s.e.m. ( n = 3 experiments, with at least 85 cells quantified per condition, per experiment). U2OS:GFP-parkin cells were treated as in (A) for the indicated time period, then fixed and immunostained for TOM20 (red). Cell boundaries are delineated in single-channel images. Scale bars, 20 μm. Source data are available online for this figure.

    Journal: The EMBO Journal

    Article Title: Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control

    doi: 10.1002/embj.201385902

    Figure Lengend Snippet: Cell-wide clearance of mitochondria via autophagy is triggered by depolarization and proceeds over a 24-h time period Representative immunoblot of whole-cell lysates from U2OS:GFP and GFP-parkin cells treated with DMSO, 25 μM antimycin A (anti A), 25 μM antimycin A with 10 μM oligomycin (anti A + oligo), or 20 μM CCCP for the indicated time period. Quantification of PDH E1a signal intensity relative to that of actin in immunoblots from (A). Error bars represent the mean ± s.e.m. ( n = 3 experiments). Quantification of mitochondrial clearance in U2OS:GFP-parkin cells treated as in (A), fixed and immunostained for TOM20. Data are shown as percentage of cells containing mitochondria, by TOM20 staining, visualized by fluorescence microscopy. Error bars represent the mean ± s.e.m. ( n = 3 experiments, with at least 85 cells quantified per condition, per experiment). U2OS:GFP-parkin cells were treated as in (A) for the indicated time period, then fixed and immunostained for TOM20 (red). Cell boundaries are delineated in single-channel images. Scale bars, 20 μm. Source data are available online for this figure.

    Article Snippet: 1:1,000), anti-beclin-1 (BD Biosciences, 612113, 1:1,000), anti-Drp1 (BD, 611113, 1:5,000), anti-GAPDH (Novus Biologicals, NB300-320, 1:50,000), anti-parkin (Santa Cruz Biotechnology, sc-32282, 1:2,000 [endogenous parkin] or 1:100,000 [ectopic parkin]), anti-PINK1 (Novus, BC100-494, 1:2,500), anti-Rab9 (Abcam, ab2810, 1:1,000), anti-TIM23 (BD, 611222, 1:40,000), anti-TOM20 (Santa Cruz Biotechnology, sc-11415, 1:50,000), anti-p97/VCP (Abcam, ab11433, 1:10,000), anti-PDH E1a (Abcam, ab110330, 1:5,000), anti-TRAP1 (Abcam, ab2721, 1:5,000), anti-α-tubulin (Santa Cruz Biotechnology, sc-23948, 1:50,000) and anti-VDAC1 (Abcam, ab14734, 1:40,000).

    Techniques: Western Blot, Staining, Fluorescence, Microscopy

    A model of microbial regulation of glucose metabolism in host colonocytes. Microbes increase blood glucose levels and increase PDH levels and activity to facilitate oxidative metabolism of glucose in the mitochondria. In contrast, GF mice are hypoglycemic, and their colonocytes respond by increased expression of GLUTs. Although this leads to increased glucose uptake, Glu-6-P and pyruvate levels are diminished because of their conversion to lactate, which is increased in GF colonocytes. This increase in glycolysis can be attributed to decreased PDH expression/activity and decreased oxidative metabolism in the mitochondria. Not only are PDH levels decreased, but also enzymatic activity is inhibited via phosphorylation of the E1a regulatory subunit due to increased expression of PDK2 in GF colonocytes.

    Journal: PLoS ONE

    Article Title: Microbial Regulation of Glucose Metabolism and Cell-Cycle Progression in Mammalian Colonocytes

    doi: 10.1371/journal.pone.0046589

    Figure Lengend Snippet: A model of microbial regulation of glucose metabolism in host colonocytes. Microbes increase blood glucose levels and increase PDH levels and activity to facilitate oxidative metabolism of glucose in the mitochondria. In contrast, GF mice are hypoglycemic, and their colonocytes respond by increased expression of GLUTs. Although this leads to increased glucose uptake, Glu-6-P and pyruvate levels are diminished because of their conversion to lactate, which is increased in GF colonocytes. This increase in glycolysis can be attributed to decreased PDH expression/activity and decreased oxidative metabolism in the mitochondria. Not only are PDH levels decreased, but also enzymatic activity is inhibited via phosphorylation of the E1a regulatory subunit due to increased expression of PDK2 in GF colonocytes.

    Article Snippet: Antibodies were used at 1∶250 to 1∶2000 and included AMPKα (Cell Signaling, Danvers, MA), phospho-AMPKα (Cell Signaling), pyruvate dehydrogenase cocktail (Mitosciences, Eugene, OR; MSP02), phospho-PDH-E1a (Abcam; ab92696), and β-actin (Abcam; ab8226).

    Techniques: Activity Assay, Mouse Assay, Expressing