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Biotechnology Information pdb protein data bank
Pdb Protein Data Bank, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Sequencing:

Article Title: Analysis of glycoside hydrolases from oat (Avena sativa) seedling extract
Article Snippet: .. Abbreviations: ESTs: Expressed Sequence Tags; TSA: Transcriptome Shotgun Assembly; AsChi: Avena sativa Chitinase; LC-MS: Liquid Chromatography-Mass Spectrometry; ORF: Open Reading Frame; SDS-PAGE: Sodium Dodecyl Sulfate-Polyacrylamide Gel; PDB: Protein Data Bank; GO: Gene Ontology; MEGA: Molecular Evolutionary Genetics Analysis; BLAST: Basic Local Alignment Search Tool; NCBI: National Center for Biotechnology Information; MEME: Multiple Expectation Maximization for Motif Elicitation; SOPMA: Self-Optimized Prediction from Multiple Alignment. ..

Thin Layer Chromatography:

Article Title: Role of Allosteric Switch Residue Histidine 195 in Maintaining Active-Site Asymmetry in Presynaptic Filaments of Bacteriophage T4 UvsX Recombinase
Article Snippet: .. ssDNA single-stranded DNA EcRecA Escherichia coli RecA ScRad51 Saccharomyces cerevisiae Rad51 dsDNA double-stranded DNA RFIII replicative fragment III TLC thin-layer chromatography PPi inorganic pyrophosphate Pi inorganic phosphate EDTA ethylenediaminetetraacetic acid NCBI National Center for Biotechnology Information PDB Protein Data Bank ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: Analysis of glycoside hydrolases from oat (Avena sativa) seedling extract
Article Snippet: .. Abbreviations: ESTs: Expressed Sequence Tags; TSA: Transcriptome Shotgun Assembly; AsChi: Avena sativa Chitinase; LC-MS: Liquid Chromatography-Mass Spectrometry; ORF: Open Reading Frame; SDS-PAGE: Sodium Dodecyl Sulfate-Polyacrylamide Gel; PDB: Protein Data Bank; GO: Gene Ontology; MEGA: Molecular Evolutionary Genetics Analysis; BLAST: Basic Local Alignment Search Tool; NCBI: National Center for Biotechnology Information; MEME: Multiple Expectation Maximization for Motif Elicitation; SOPMA: Self-Optimized Prediction from Multiple Alignment. ..

Chromatography:

Article Title: Analysis of glycoside hydrolases from oat (Avena sativa) seedling extract
Article Snippet: .. Abbreviations: ESTs: Expressed Sequence Tags; TSA: Transcriptome Shotgun Assembly; AsChi: Avena sativa Chitinase; LC-MS: Liquid Chromatography-Mass Spectrometry; ORF: Open Reading Frame; SDS-PAGE: Sodium Dodecyl Sulfate-Polyacrylamide Gel; PDB: Protein Data Bank; GO: Gene Ontology; MEGA: Molecular Evolutionary Genetics Analysis; BLAST: Basic Local Alignment Search Tool; NCBI: National Center for Biotechnology Information; MEME: Multiple Expectation Maximization for Motif Elicitation; SOPMA: Self-Optimized Prediction from Multiple Alignment. ..

SDS Page:

Article Title: Analysis of glycoside hydrolases from oat (Avena sativa) seedling extract
Article Snippet: .. Abbreviations: ESTs: Expressed Sequence Tags; TSA: Transcriptome Shotgun Assembly; AsChi: Avena sativa Chitinase; LC-MS: Liquid Chromatography-Mass Spectrometry; ORF: Open Reading Frame; SDS-PAGE: Sodium Dodecyl Sulfate-Polyacrylamide Gel; PDB: Protein Data Bank; GO: Gene Ontology; MEGA: Molecular Evolutionary Genetics Analysis; BLAST: Basic Local Alignment Search Tool; NCBI: National Center for Biotechnology Information; MEME: Multiple Expectation Maximization for Motif Elicitation; SOPMA: Self-Optimized Prediction from Multiple Alignment. ..

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    Biotechnology Information protein data bank pdb
    In silico , Red, UbcH7; green, cbl; yellow, <t>RAG1;</t> magenta spheres, cbl zinc ions; grey spheres, RAG1 zinc ions. Protein Data Bank <t>(PDB)</t> ID for the cbl–ubcH7 co-structure is 1fbv, PDB ID for RAG1 RING is 1rmd.
    Protein Data Bank Pdb, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein data bank pdb/product/Biotechnology Information
    Average 91 stars, based on 10 article reviews
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    Biotechnology Information wt tlr2 structure
    Expression levels and signaling capacities of WT and R753Q <t>YFP-TLR2</t> species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,
    Wt Tlr2 Structure, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    In silico , Red, UbcH7; green, cbl; yellow, RAG1; magenta spheres, cbl zinc ions; grey spheres, RAG1 zinc ions. Protein Data Bank (PDB) ID for the cbl–ubcH7 co-structure is 1fbv, PDB ID for RAG1 RING is 1rmd.

    Journal: Immunology

    Article Title: Correlation between recombinase activating gene 1 ubiquitin ligase activity and V(D)J recombination

    doi: 10.1111/j.1365-2567.2009.03101.x

    Figure Lengend Snippet: In silico , Red, UbcH7; green, cbl; yellow, RAG1; magenta spheres, cbl zinc ions; grey spheres, RAG1 zinc ions. Protein Data Bank (PDB) ID for the cbl–ubcH7 co-structure is 1fbv, PDB ID for RAG1 RING is 1rmd.

    Article Snippet: The interface between CDC34 and RAG1 was predicted based on in silico alignment of the RAG1 RING and cbl-UbcH7 crystal structures obtained using the Protein Data Bank (PDB) co-ordinates available from the National Center for Biotechnology Information (NCBI) structural database., Initial manual alignments allowed us to designate 32 residue pairs in equivalent positions in the two structures, and these were aligned using the least square fit protocol of the align program (Shareware).

    Techniques: In Silico

    Overlay of the protein structures of human cathepsin V, PDB 1FH0 [ 26 ] (red ribbon), and the homology model of Trichomonas vaginalis cathepsin L-like protein, TvCPCAC1 (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Overlay of the protein structures of human cathepsin V, PDB 1FH0 [ 26 ] (red ribbon), and the homology model of Trichomonas vaginalis cathepsin L-like protein, TvCPCAC1 (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques:

    Overlay of the protein structures of Brucella melitensis TxR, PDB 4JNQ [ 24 ] (red ribbon), and the homology model of Trichomonas vaginalis TxR (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Overlay of the protein structures of Brucella melitensis TxR, PDB 4JNQ [ 24 ] (red ribbon), and the homology model of Trichomonas vaginalis TxR (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques:

    Ramachandran plots of cathepsin V-like protein structures: ( a ) human cathepsin V (HsCatV, PDB 1FH0 [ 26 ]); ( b ) homology model of Trichomonas vaginalis cathepsin L-like protein, TvCPCAC1; ( c ) binding site of HsCatV; ( d ) binding site of TvCPCAC1.

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Ramachandran plots of cathepsin V-like protein structures: ( a ) human cathepsin V (HsCatV, PDB 1FH0 [ 26 ]); ( b ) homology model of Trichomonas vaginalis cathepsin L-like protein, TvCPCAC1; ( c ) binding site of HsCatV; ( d ) binding site of TvCPCAC1.

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques: Binding Assay

    Ramachandran plots of cathepsin K-like protein structures: ( a ) rabbit ( Oryctolagus cuniculus ) cathepsin K (OcCatK, PDB 2F7D [ 25 ]); ( b ) homology model of Trichomonas vaginalis papain-like protein, TvCP2; ( c ) binding site of OcCatK; ( d ) binding site of TvCP2.

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Ramachandran plots of cathepsin K-like protein structures: ( a ) rabbit ( Oryctolagus cuniculus ) cathepsin K (OcCatK, PDB 2F7D [ 25 ]); ( b ) homology model of Trichomonas vaginalis papain-like protein, TvCP2; ( c ) binding site of OcCatK; ( d ) binding site of TvCP2.

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques: Binding Assay

    Lowest-energy re-docked poses of co-crystallized ligands: ( a ) Trichomonas vaginalis methionine gamma-lyase (TvMGL, PDB 1E5F [ 27 ]) showing the co-crystallized ligand, pyridoxal-5′-phosphate (green), and the re-docked ligand (magenta); ( b ) T. vaginalis purine nucleoside phosphorylase (TvPNP, PDB 1Z36 [ 28 ]) showing the co-crystallized ligand, (1 S )-1-(7-amino-1 H -pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro- d -ribitol (green), and the re-docked ligand (red); ( c ) human cathepsin K (HsCatK, PDB 1U9V [ 38 ]) showing the co-crystallized ligand, 6-(cyclohexylamino)-9-[2-(4-methylpiperazin-1-yl)-ethyl]-9 H -purine-2-carbonitrile (green), and the re-docked ligand (blue); and ( d ) human cathepsin L (HsCatL, PDB 3HWN [ 39 ]) showing the co-crystallized ligand, N -α-[(3- t -Butyl-1-methyl-1 H -pyrazol-5-yl)carbonyl]- N -[(2 E )-2-iminoethyl]-3-{5-[( Z )-iminomethyl]-1,3,4-oxadiazol-2-yl}- l -phenylalaninamide (green), and the re-docked ligand (aqua).

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Lowest-energy re-docked poses of co-crystallized ligands: ( a ) Trichomonas vaginalis methionine gamma-lyase (TvMGL, PDB 1E5F [ 27 ]) showing the co-crystallized ligand, pyridoxal-5′-phosphate (green), and the re-docked ligand (magenta); ( b ) T. vaginalis purine nucleoside phosphorylase (TvPNP, PDB 1Z36 [ 28 ]) showing the co-crystallized ligand, (1 S )-1-(7-amino-1 H -pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro- d -ribitol (green), and the re-docked ligand (red); ( c ) human cathepsin K (HsCatK, PDB 1U9V [ 38 ]) showing the co-crystallized ligand, 6-(cyclohexylamino)-9-[2-(4-methylpiperazin-1-yl)-ethyl]-9 H -purine-2-carbonitrile (green), and the re-docked ligand (blue); and ( d ) human cathepsin L (HsCatL, PDB 3HWN [ 39 ]) showing the co-crystallized ligand, N -α-[(3- t -Butyl-1-methyl-1 H -pyrazol-5-yl)carbonyl]- N -[(2 E )-2-iminoethyl]-3-{5-[( Z )-iminomethyl]-1,3,4-oxadiazol-2-yl}- l -phenylalaninamide (green), and the re-docked ligand (aqua).

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques:

    Overlay of the protein structures of rabbit ( Oryctolagus cuniculus ) cathepsin K, PDB 2F7D [ 25 ] (red ribbon), and the homology model of Trichomonas vaginalis papain-like protein, TvCP2 (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Journal: Scientia Pharmaceutica

    Article Title: Natural Products as New Treatment Options for Trichomoniasis: A Molecular Docking Investigation

    doi: 10.3390/scipharm85010005

    Figure Lengend Snippet: Overlay of the protein structures of rabbit ( Oryctolagus cuniculus ) cathepsin K, PDB 2F7D [ 25 ] (red ribbon), and the homology model of Trichomonas vaginalis papain-like protein, TvCP2 (blue ribbon). The co-crystallized ligand is shown as a wireframe structure.

    Article Snippet: Homology Modeling Homology models for each of the Trichomonas proteins that are not currently available from the Protein Data Bank (PDB) were constructed from crystal structure templates found in the Protein Data Bank using FASTA sequences downloaded from the National Center for Biotechnology Information’s (NCBI) GenBank.

    Techniques:

    Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: Expression levels and signaling capacities of WT and R753Q YFP-TLR2 species. A–D , HEK293 cells were transiently ( A , C , and D ) or stably ( B ) transfected with expression vectors encoding WT or R753Q YFP-TLR2 variants. A , after recovery for 48 h,

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing, Stable Transfection, Transfection

    The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A , HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing, Stable Transfection, Transfection

    Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: Molecular modeling of the structures of the TIR domains of WT and R753Q TIR2 species. A–C , ribbon models of WT Arg-753 ( A ) and mutant Gln-753 ( MUT Q753 ) ( B ) TIR domains of TLR2 and their overlay ( C ) are shown. D , a close-up of the DD-loop-αD

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Mutagenesis

    The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The impact of R753Q polymorphism on TLR2-mediated phosphorylation of p38 and IκB-α and degradation of IκB-α. WT or R753Q YFP-TLR2 variants were overexpressed in HEK293T cells following transient transfection of the corresponding

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Transfection

    The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The R753Q polymorphism impairs the ability of TLR2 to hetero-dimerize with TLR6 upon Pam2Cys stimulation. A , 293/YFP-TLR2 stable cell lines expressing WT or R753Q YFP-TLR2 variants were co-transfected with pcDNA3-CFP-TLR6. After recovery for 20 h, cells

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Stable Transfection, Expressing, Transfection

    R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: R753Q TLR2 shows deficient capacity to recruit Mal and MyD88 in response to agonist stimulation. HEK293T cells were transiently transfected with pEFBOS-Flag-Mal ( A ) or pcDNA3-AU1-MyD88 ( B ) in combination with pcDNA3-WT YFP-TLR2 or pcDNA3-R753Q YFP-TLR2,

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Transfection

    The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: The impact of R753Q polymorphism on agonist-induced tyrosine phosphorylation of TLR2. A and B , HEK293 stable transfectants expressing WT or R753Q TLR2 were stimulated for the indicated time points with 100 ng/ml Pam3Cys ( A ) or treated for 15 min with

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Expressing

    R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected

    Journal: The Journal of Biological Chemistry

    Article Title: R753Q Polymorphism Inhibits Toll-like Receptor (TLR) 2 Tyrosine Phosphorylation, Dimerization with TLR6, and Recruitment of Myeloid Differentiation Primary Response Protein 88 *

    doi: 10.1074/jbc.M112.375493

    Figure Lengend Snippet: R753Q TLR2 mediates impaired activation of NF-κB luciferase reporter in response to inactivated M. tuberculosis , S. aureus , mycobacteria-derived cell wall, and culture filtrate fractions, LAM and Pam3Cys. HEK293T cells were transiently transfected

    Article Snippet: Structural models of the WT and R753Q-containing TIR domains of TLR2 were built using the WT TLR2 structure (Protein Data Bank (PDB) accession code 1FYW ) and the 3D-JIGSAW Protein Comparative Modeling Server (version 2.0) and visualized and analyzed using the Cn3D 4.3 macromolecular structure viewer (National Center for Biotechnology Information (NCBI), ) and PyMOL.

    Techniques: Activation Assay, Luciferase, Derivative Assay, Laser Capture Microdissection, Transfection