human dermal fibroblasts  (ATCC)


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    ATCC human dermal fibroblasts
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pcs 201 012 fibroblasts  (ATCC)


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    ATCC pcs 201 012 fibroblasts
    Pcs 201 012 Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human dermal fibroblasts  (ATCC)


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    ATCC human dermal fibroblasts
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    early passage human primary dermal fibroblasts  (ATCC)


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    ATCC early passage human primary dermal fibroblasts
    Early Passage Human Primary Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human dermal fibroblasts hdfs  (ATCC)


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    ATCC human dermal fibroblasts hdfs
    Human Dermal Fibroblasts Hdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human dermal fibroblasts  (ATCC)


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    ATCC human dermal fibroblasts
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    adult dermal  (ATCC)


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    ATCC adult dermal
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    human dermal fibroblast cells  (ATCC)


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    ATCC human dermal fibroblast cells
    Human Dermal Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human dermal fibroblasts  (ATCC)


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    ATCC human dermal fibroblasts
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    human dermal fibroblast hdf cells  (ATCC)


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    ATCC human dermal fibroblast hdf cells
    Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human <t>dermal</t> <t>fibroblast</t> cells. ( A ) <t>HDF</t> cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.
    Human Dermal Fibroblast Hdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening"

    Article Title: Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening

    Journal: Antioxidants

    doi: 10.3390/antiox12010051

    Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. ( A ) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.
    Figure Legend Snippet: Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. ( A ) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

    Techniques Used: Irradiation, MTT Assay, Western Blot, Enzyme-linked Immunosorbent Assay

    Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. ( A – C ) The ROS production was analyzed by flow cytometer. ( D – F ) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ### p < 0.001 vs. non-treated (NT) cells, ## p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.
    Figure Legend Snippet: Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. ( A – C ) The ROS production was analyzed by flow cytometer. ( D – F ) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ### p < 0.001 vs. non-treated (NT) cells, ## p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.

    Techniques Used: Irradiation, Flow Cytometry

    human dermal fibroblasts  (ATCC)


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    ATCC human dermal fibroblasts
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human dermal fibroblasts
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    ATCC pcs 201 012 fibroblasts
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    ATCC early passage human primary dermal fibroblasts
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    ATCC human dermal fibroblasts hdfs
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    ATCC human dermal fibroblast cells
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    ATCC human dermal fibroblast hdf cells
    Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human <t>dermal</t> <t>fibroblast</t> cells. ( A ) <t>HDF</t> cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.
    Human Dermal Fibroblast Hdf Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. ( A ) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

    Journal: Antioxidants

    Article Title: Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening

    doi: 10.3390/antiox12010051

    Figure Lengend Snippet: Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. ( A ) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. ( B ) HDF cells were irradiated with UVB (100 mJ/cm 2 ) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. ( C ) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm 2 ) irradiation. The RNA level was evaluated using reverse transcription PCR. ( D ) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

    Article Snippet: Human dermal fibroblast (HDF) cells and mouse skin melanoma B16F10 cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Irradiation, MTT Assay, Western Blot, Enzyme-linked Immunosorbent Assay

    Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. ( A – C ) The ROS production was analyzed by flow cytometer. ( D – F ) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ### p < 0.001 vs. non-treated (NT) cells, ## p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.

    Journal: Antioxidants

    Article Title: Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening

    doi: 10.3390/antiox12010051

    Figure Lengend Snippet: Antioxidant effects of YJP-EA, -Hex, and -BuOH on HaCaT, HDF, and B16F10 cells. HaCaT and HDF cells were irradiated with UVB; B16F10 cells were stimulated by α-MSH. We then treated them with NAC (3 mM) for 15 min or with YJP-EA, -Hex, and -BuOH (50 μg/mL) for 12 h. ( A – C ) The ROS production was analyzed by flow cytometer. ( D – F ) The GSH and GSSG levels were measured, and the GSSG/GSH ratio was evaluated in both cells. All experiments were performed individually in triplicate. ### p < 0.001 vs. non-treated (NT) cells, ## p < 0.01 vs. non-treated (NT) cells, *** p < 0.001 vs. UVB or α-MSH-stimulated cells, ** p < 0.01 vs. UVB or α-MSH-stimulated cells, and * p < 0.05 vs. UVB or α-MSH-stimulated cells.

    Article Snippet: Human dermal fibroblast (HDF) cells and mouse skin melanoma B16F10 cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Irradiation, Flow Cytometry