pcr4 expression vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr4 expression vector
    Pcr4 Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 expression vector/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr4 expression vector - by Bioz Stars, 2019-10
    87/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    Amplification:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies).

    In Vitro:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Paragraph title: DNA plasmids and in vitro translation ... Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies).

    TA Cloning:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    Construct:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Each DNA plasmid was then used as template for 5’-end insertion of Hemagglutinin (HA) tag consensus coding sequence (-TACCCATACGATGTTCCAGATTACGCT- , 27 base pairs) into the complete open reading frame of rat Mesothelin coding sequence, by overlap PCR extension.

    Purification:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    Polymerase Chain Reaction:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    DNA Sequencing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    Expressing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

    Sequencing:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: RGF-N2 cDNA sample was PCR-amplified using primers located in the 5’- and 3’-UTR regions of rat Mesothelin coding sequence (NCBI Nucleotide ID: NM_031658.1). .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies).

    Hemagglutination Assay:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. After PCR analysis of approximately 200 obtained transformants, six clones (A, H, S, U, W, Y) were selected based upon PCR amplicon size/abundance (ranging between 300 and 2100 base pairs approximately), and analyzed by automated sequencing to confirm insert size, sequence and orientation (UAMS DNA Sequencing Core Facility).

    Plasmid Preparation:

    Article Title: Liver myofibroblasts of murine origins express mesothelin: Identification of novel rat mesothelin splice variants*
    Article Snippet: All PCR reactions were performed with Phusion® (New England BioLabs) or TopTaq® High-Fidelity DNA polymerases for maximal elongation fidelity. .. Purified PCR reactions products were cloned into the pCR4® expression vector (Life Technologies), using a TOPO® TA cloning kit (Life Technologies). .. Chemically-competent OneShot® TOP10 bacteria cells (Life Technologies) were used for superior transformation efficiency.

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    Thermo Fisher vector pcr4 topo
    Resistance against sulfonamide antibiotics mediated by SEW2_dhps01, SEW5_dhps01, AEW9_dhps01 , and AEG2_dhps01 . Five microliters of serially diluted E. coli TOP10 cultures with starting OD 600 of 0.5 were spotted onto Iso-Sensitest agar plates supplemented with 1000 mg/L sulfamethazine (+ SMZ), 250 mg/L sulfamethoxazole (+ SMX), 250 mg/L sulfadiazine (+ SDZ) or 500 mg/L sulfisoxazole (+ SOX). Iso-Sensitest agar plates with no sulfonamide added (control) were also included. E. coli TOP10 cultures carrying the cloning vector <t>pCR4-TOPO,</t> pCR4_SEW2_dhps01, pCR4_SEW5_dhps01, pCR4_AEW9_dhps01 or pCR4_AEG2_dhps01 were considered.
    Vector Pcr4 Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pcr4 topo/product/Thermo Fisher
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    vector pcr4 topo - by Bioz Stars, 2019-10
    99/100 stars
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    Resistance against sulfonamide antibiotics mediated by SEW2_dhps01, SEW5_dhps01, AEW9_dhps01 , and AEG2_dhps01 . Five microliters of serially diluted E. coli TOP10 cultures with starting OD 600 of 0.5 were spotted onto Iso-Sensitest agar plates supplemented with 1000 mg/L sulfamethazine (+ SMZ), 250 mg/L sulfamethoxazole (+ SMX), 250 mg/L sulfadiazine (+ SDZ) or 500 mg/L sulfisoxazole (+ SOX). Iso-Sensitest agar plates with no sulfonamide added (control) were also included. E. coli TOP10 cultures carrying the cloning vector pCR4-TOPO, pCR4_SEW2_dhps01, pCR4_SEW5_dhps01, pCR4_AEW9_dhps01 or pCR4_AEG2_dhps01 were considered.

    Journal: Frontiers in Microbiology

    Article Title: Discovery of Novel Antibiotic Resistance Determinants in Forest and Grassland Soil Metagenomes

    doi: 10.3389/fmicb.2019.00460

    Figure Lengend Snippet: Resistance against sulfonamide antibiotics mediated by SEW2_dhps01, SEW5_dhps01, AEW9_dhps01 , and AEG2_dhps01 . Five microliters of serially diluted E. coli TOP10 cultures with starting OD 600 of 0.5 were spotted onto Iso-Sensitest agar plates supplemented with 1000 mg/L sulfamethazine (+ SMZ), 250 mg/L sulfamethoxazole (+ SMX), 250 mg/L sulfadiazine (+ SDZ) or 500 mg/L sulfisoxazole (+ SOX). Iso-Sensitest agar plates with no sulfonamide added (control) were also included. E. coli TOP10 cultures carrying the cloning vector pCR4-TOPO, pCR4_SEW2_dhps01, pCR4_SEW5_dhps01, pCR4_AEW9_dhps01 or pCR4_AEG2_dhps01 were considered.

    Article Snippet: To verify if candidate ORFs encode antibiotic resistance, they were subcloned into vector pCR4-TOPO (Thermo Fisher Scientific) and subsequently introduced into E. coli TOP10.

    Techniques: Clone Assay, Plasmid Preparation

    Antibiotic susceptibility profiles of E. coli TOP10 carrying soil-derived genes involved in antibiotic resistance. The genes were subcloned into plasmid vector pCR4-TOPO. MICs of antibiotics were determined using the broth microdilution method and are presented as fold increase relative to those for E. coli TOP10 carrying the cloning vector pCR4-TOPO. CAX, cefotaxime; CHL, chloramphenicol; ERY, erythromycin; GEN, gentamicin; LIN, lincomycin; RIF, rifampicin; SDZ, sulfadiazine; SMX, sulfamethoxazole; SMZ, sulfamethazine; SOX, sulfisoxazole; TET, tetracycline; TYL, tylosin.

    Journal: Frontiers in Microbiology

    Article Title: Discovery of Novel Antibiotic Resistance Determinants in Forest and Grassland Soil Metagenomes

    doi: 10.3389/fmicb.2019.00460

    Figure Lengend Snippet: Antibiotic susceptibility profiles of E. coli TOP10 carrying soil-derived genes involved in antibiotic resistance. The genes were subcloned into plasmid vector pCR4-TOPO. MICs of antibiotics were determined using the broth microdilution method and are presented as fold increase relative to those for E. coli TOP10 carrying the cloning vector pCR4-TOPO. CAX, cefotaxime; CHL, chloramphenicol; ERY, erythromycin; GEN, gentamicin; LIN, lincomycin; RIF, rifampicin; SDZ, sulfadiazine; SMX, sulfamethoxazole; SMZ, sulfamethazine; SOX, sulfisoxazole; TET, tetracycline; TYL, tylosin.

    Article Snippet: To verify if candidate ORFs encode antibiotic resistance, they were subcloned into vector pCR4-TOPO (Thermo Fisher Scientific) and subsequently introduced into E. coli TOP10.

    Techniques: Derivative Assay, Plasmid Preparation, Clone Assay