evagreen  (Jena Bioscience)


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    Name:
    EvaGreen Fluorescent DNA Stain
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    Catalog Number:
    PCR-379
    Price:
    44.1
    Category:
    Molecular Biology
    Size:
    500 µl
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    Structured Review

    Jena Bioscience evagreen

    https://www.bioz.com/result/evagreen/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    evagreen - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Sandwich ELISA:

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study
    Article Snippet: Mixtures of trypsinized cells were resuspended in 1 ml ice-cold lysis buffer (0.1% Tween 20, complete EDTA-free protease inhibitors [Roche Diagnostics, Germany], 1× PBS) and frozen at −80°C. .. Resulting supernatants were applied to sandwich ELISA or used for measurement of green fluorescence after addition of EvaGreen DNA stain (Jena Bioscience, Jena, Germany). .. The clinical samples were thawed immediately before use, and 20 μl of 50× protease inhibitor stock solution (complete EDTA-free protease inhibitors; Roche Diagnostics, Germany) was added per collecting tube (1 ml sample).

    Fluorescence:

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study
    Article Snippet: Mixtures of trypsinized cells were resuspended in 1 ml ice-cold lysis buffer (0.1% Tween 20, complete EDTA-free protease inhibitors [Roche Diagnostics, Germany], 1× PBS) and frozen at −80°C. .. Resulting supernatants were applied to sandwich ELISA or used for measurement of green fluorescence after addition of EvaGreen DNA stain (Jena Bioscience, Jena, Germany). .. The clinical samples were thawed immediately before use, and 20 μl of 50× protease inhibitor stock solution (complete EDTA-free protease inhibitors; Roche Diagnostics, Germany) was added per collecting tube (1 ml sample).

    Staining:

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study
    Article Snippet: Mixtures of trypsinized cells were resuspended in 1 ml ice-cold lysis buffer (0.1% Tween 20, complete EDTA-free protease inhibitors [Roche Diagnostics, Germany], 1× PBS) and frozen at −80°C. .. Resulting supernatants were applied to sandwich ELISA or used for measurement of green fluorescence after addition of EvaGreen DNA stain (Jena Bioscience, Jena, Germany). .. The clinical samples were thawed immediately before use, and 20 μl of 50× protease inhibitor stock solution (complete EDTA-free protease inhibitors; Roche Diagnostics, Germany) was added per collecting tube (1 ml sample).

    Article Title: Abundance and potential contribution of Gram-negative cheese rind bacteria from Austrian artisanal hard cheeses.
    Article Snippet: Each optimized qPCR reaction was run in duplicate 169 with a final volume of 25 µL, using MicroAmp 0.2 mL optical tubes sealed with MicroAmp optical 170 8-cap strips (Applied Biosystems, Foster City, CA, USA). .. Single amplification reactions for 171 Advenella, Psychrobacter and Psychroflexus qPCRs consisted of 12.2 µL diethylpyrocarbonate 172 (DEPC) -treated water, 2.5 µL 10×buffer, 1.5 µL 3 mM MgCl2, 1 µL 400 nM of each primer, 0.5 173 µL undiluted EvaGreen® fluorescent DNA stain (Jena Bioscience, Jena, Germany), 1 µL 200 mM 174 of each dNTP, 0.3 µL 1.5U of Platinum® Taq DNA polymerase (Thermo Fisher Scientific, 175 Vienna, Austria) and 5 µL template (genomic or plasmid DNA). .. The quantification of DNA was 176 performed in Mx3000P™ qPCR instrument (Stratagene, La Jolla, CA, USA) (software v.4.10) 177 after initial denaturation at 94°C for two min, followed by 45 cycles of 94°C for 30 sec, 60°C for 178 9 one min.

    Article Title: Rapid identification of Borrelia by high resolution melting analysis of the groEL gene.
    Article Snippet: .. The reaction mixture contained: 70 mM Tris-HCl (pH 8.6; 25°C), 16.6 mM (NH4)2SO4, 0.75 mM of deoxyribonucleotide triphosphate (dNTP), 4 pmol of gro-f4N and gro-r1N primers, 3.5 mM MgCl2, 0.5 U Allegro Taq DNA polimerase (Novazym, Poland), 0.4Fl of EvaGreen stain (Jena Bioscience, Germany) and 1 Fl of DNA suspension isolated from mammalian blood or collected ticks. ..

    Article Title: Conserved Gene Regulatory Function of the Carboxy-Terminal Domain of Dictyostelid C-Module-Binding Factor
    Article Snippet: Total RNA was prepared from frozen pellets consisting of 2 × 107 cells with the Qiagen RNeasy Minikit according to the protocol provided. cDNA was synthesized by the RT of 500 ng of total RNA with an oligo(dT) primer and the Qiagen Omniscript RT kit. .. Real-time amplification of the genes was performed with the Taq polymerase mix from Jena Bioscience (Jena, Germany) supplemented with EvaGreen Fluorescent Gel Stain and ROX Reference Dye (both from Jena Bioscience, Jena, Germany) on a Stratagene Mx3000P instrument. ..

    Article Title: Evaluating the Evidence for Transmission Distortion in Human Pedigrees
    Article Snippet: .. A 1914-bp region containing the three SNPs was amplified using the following PCR conditions: 1× Phire HS buffer (Biozym, Austria), 0.16 mM dNTPs, 0.8 µM forward (5′-AGCCTCTTGTGCCAAACAGT-3′) and 0.8 µM reverse primers (5′-TTTTTGCTGGCAGAGGATCT-3′), 0.5× EvaGreen fluorescent DNA stain (Jena Bioscience, Germany), 0.25 µl Phire Hot Start DNA polymerase (Biozym, Austria), and 0.3–0.6 molecules of blood or sperm DNA per reaction. .. This amount of template ensured that < 10% of the reactions had more than one molecule amplified, according to the Poisson distribution.

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study
    Article Snippet: .. The autofluorescence of the lysate was measured, 2 μl 50× EvaGreen DNA stain solution (Jena Bioscience, Jena, Germany) was added, and the plate was incubated for 10 min in the dark at room temperature. .. Subsequently, the EvaGreen fluorescence was measured.

    Article Title: Hepatoprotective effects of vitamin E/selenium against malathion-induced injuries on the antioxidant status and apoptosis-related gene expression in rats.
    Article Snippet: Expression levels were normalized to GAPDH gene expression, which was used as an internal housekeeping control. .. Real-time quantification was performed in the LightCycler® 480 Instrument with 96-well plate (Roche Diagnostics GmbH, Mannheim, Germany) using the qPCR GreenMaster based on EvaGreen Fluorescent DNA Stain (Jena Bioscience GmbH, Germany). .. PCR mixtures (final volume of 20 μl) contained 10 μl of qPCR GreenMaster (Jena Bioscience), 5 μl of a 10-2 dilution of the cDNA and 300 nM of each primer (Table 1).

    Amplification:

    Article Title: Abundance and potential contribution of Gram-negative cheese rind bacteria from Austrian artisanal hard cheeses.
    Article Snippet: Each optimized qPCR reaction was run in duplicate 169 with a final volume of 25 µL, using MicroAmp 0.2 mL optical tubes sealed with MicroAmp optical 170 8-cap strips (Applied Biosystems, Foster City, CA, USA). .. Single amplification reactions for 171 Advenella, Psychrobacter and Psychroflexus qPCRs consisted of 12.2 µL diethylpyrocarbonate 172 (DEPC) -treated water, 2.5 µL 10×buffer, 1.5 µL 3 mM MgCl2, 1 µL 400 nM of each primer, 0.5 173 µL undiluted EvaGreen® fluorescent DNA stain (Jena Bioscience, Jena, Germany), 1 µL 200 mM 174 of each dNTP, 0.3 µL 1.5U of Platinum® Taq DNA polymerase (Thermo Fisher Scientific, 175 Vienna, Austria) and 5 µL template (genomic or plasmid DNA). .. The quantification of DNA was 176 performed in Mx3000P™ qPCR instrument (Stratagene, La Jolla, CA, USA) (software v.4.10) 177 after initial denaturation at 94°C for two min, followed by 45 cycles of 94°C for 30 sec, 60°C for 178 9 one min.

    Article Title: Conserved Gene Regulatory Function of the Carboxy-Terminal Domain of Dictyostelid C-Module-Binding Factor
    Article Snippet: Total RNA was prepared from frozen pellets consisting of 2 × 107 cells with the Qiagen RNeasy Minikit according to the protocol provided. cDNA was synthesized by the RT of 500 ng of total RNA with an oligo(dT) primer and the Qiagen Omniscript RT kit. .. Real-time amplification of the genes was performed with the Taq polymerase mix from Jena Bioscience (Jena, Germany) supplemented with EvaGreen Fluorescent Gel Stain and ROX Reference Dye (both from Jena Bioscience, Jena, Germany) on a Stratagene Mx3000P instrument. ..

    Article Title: Evaluating the Evidence for Transmission Distortion in Human Pedigrees
    Article Snippet: .. A 1914-bp region containing the three SNPs was amplified using the following PCR conditions: 1× Phire HS buffer (Biozym, Austria), 0.16 mM dNTPs, 0.8 µM forward (5′-AGCCTCTTGTGCCAAACAGT-3′) and 0.8 µM reverse primers (5′-TTTTTGCTGGCAGAGGATCT-3′), 0.5× EvaGreen fluorescent DNA stain (Jena Bioscience, Germany), 0.25 µl Phire Hot Start DNA polymerase (Biozym, Austria), and 0.3–0.6 molecules of blood or sperm DNA per reaction. .. This amount of template ensured that < 10% of the reactions had more than one molecule amplified, according to the Poisson distribution.

    Plasmid Preparation:

    Article Title: Abundance and potential contribution of Gram-negative cheese rind bacteria from Austrian artisanal hard cheeses.
    Article Snippet: Each optimized qPCR reaction was run in duplicate 169 with a final volume of 25 µL, using MicroAmp 0.2 mL optical tubes sealed with MicroAmp optical 170 8-cap strips (Applied Biosystems, Foster City, CA, USA). .. Single amplification reactions for 171 Advenella, Psychrobacter and Psychroflexus qPCRs consisted of 12.2 µL diethylpyrocarbonate 172 (DEPC) -treated water, 2.5 µL 10×buffer, 1.5 µL 3 mM MgCl2, 1 µL 400 nM of each primer, 0.5 173 µL undiluted EvaGreen® fluorescent DNA stain (Jena Bioscience, Jena, Germany), 1 µL 200 mM 174 of each dNTP, 0.3 µL 1.5U of Platinum® Taq DNA polymerase (Thermo Fisher Scientific, 175 Vienna, Austria) and 5 µL template (genomic or plasmid DNA). .. The quantification of DNA was 176 performed in Mx3000P™ qPCR instrument (Stratagene, La Jolla, CA, USA) (software v.4.10) 177 after initial denaturation at 94°C for two min, followed by 45 cycles of 94°C for 30 sec, 60°C for 178 9 one min.

    Isolation:

    Article Title: Rapid identification of Borrelia by high resolution melting analysis of the groEL gene.
    Article Snippet: .. The reaction mixture contained: 70 mM Tris-HCl (pH 8.6; 25°C), 16.6 mM (NH4)2SO4, 0.75 mM of deoxyribonucleotide triphosphate (dNTP), 4 pmol of gro-f4N and gro-r1N primers, 3.5 mM MgCl2, 0.5 U Allegro Taq DNA polimerase (Novazym, Poland), 0.4Fl of EvaGreen stain (Jena Bioscience, Germany) and 1 Fl of DNA suspension isolated from mammalian blood or collected ticks. ..

    Polymerase Chain Reaction:

    Article Title: Evaluating the Evidence for Transmission Distortion in Human Pedigrees
    Article Snippet: .. A 1914-bp region containing the three SNPs was amplified using the following PCR conditions: 1× Phire HS buffer (Biozym, Austria), 0.16 mM dNTPs, 0.8 µM forward (5′-AGCCTCTTGTGCCAAACAGT-3′) and 0.8 µM reverse primers (5′-TTTTTGCTGGCAGAGGATCT-3′), 0.5× EvaGreen fluorescent DNA stain (Jena Bioscience, Germany), 0.25 µl Phire Hot Start DNA polymerase (Biozym, Austria), and 0.3–0.6 molecules of blood or sperm DNA per reaction. .. This amount of template ensured that < 10% of the reactions had more than one molecule amplified, according to the Poisson distribution.

    Article Title: Crossovers are associated with mutation and biased gene conversion at recombination hotspots
    Article Snippet: Reactions contained genomic DNA from 100 to 600 total sperm heads (quantified via a spectrophotometer), 0.25 µM of the appropriate forward and reverse allele-specific primer, 0.16 mM dNTPs, 1× Phusion HF Buffer (ThermoFisher Scientific), and 0.1 U Phusion Hot Start II High-Fidelity DNA Polymerase, a thermostable polymerase with the lowest reported error rate ( ); primer sequences; and cycling conditions are shown in . .. The second round of PCR was carried out in a volume of 20 µL with the same components as above, but with an aliquot of 1 or 2 µL of the first PCR instead of genomic DNA and 1× EvaGreen (Jena Bioscience) in a real-time PCR system (CFX384 System, Bio-Rad). .. Reactions for the first and second round of PCR were set up in different laminar flow hoods located in separate rooms to avoid carry-over contamination.

    Real-time Polymerase Chain Reaction:

    Article Title: Crossovers are associated with mutation and biased gene conversion at recombination hotspots
    Article Snippet: Reactions contained genomic DNA from 100 to 600 total sperm heads (quantified via a spectrophotometer), 0.25 µM of the appropriate forward and reverse allele-specific primer, 0.16 mM dNTPs, 1× Phusion HF Buffer (ThermoFisher Scientific), and 0.1 U Phusion Hot Start II High-Fidelity DNA Polymerase, a thermostable polymerase with the lowest reported error rate ( ); primer sequences; and cycling conditions are shown in . .. The second round of PCR was carried out in a volume of 20 µL with the same components as above, but with an aliquot of 1 or 2 µL of the first PCR instead of genomic DNA and 1× EvaGreen (Jena Bioscience) in a real-time PCR system (CFX384 System, Bio-Rad). .. Reactions for the first and second round of PCR were set up in different laminar flow hoods located in separate rooms to avoid carry-over contamination.

    Article Title: Hepatoprotective effects of vitamin E/selenium against malathion-induced injuries on the antioxidant status and apoptosis-related gene expression in rats.
    Article Snippet: Expression levels were normalized to GAPDH gene expression, which was used as an internal housekeeping control. .. Real-time quantification was performed in the LightCycler® 480 Instrument with 96-well plate (Roche Diagnostics GmbH, Mannheim, Germany) using the qPCR GreenMaster based on EvaGreen Fluorescent DNA Stain (Jena Bioscience GmbH, Germany). .. PCR mixtures (final volume of 20 μl) contained 10 μl of qPCR GreenMaster (Jena Bioscience), 5 μl of a 10-2 dilution of the cDNA and 300 nM of each primer (Table 1).

    Incubation:

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study
    Article Snippet: .. The autofluorescence of the lysate was measured, 2 μl 50× EvaGreen DNA stain solution (Jena Bioscience, Jena, Germany) was added, and the plate was incubated for 10 min in the dark at room temperature. .. Subsequently, the EvaGreen fluorescence was measured.

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  • 93
    Jena Bioscience evagreen dna stain solution
    E7 detection in cervical carcinoma cells and cervical smears. (A) An increasing number of HPV18-positive HeLa cells (0 to 25,000 cells/well) was complemented with HPV-negative U-2OS cells to achieve a total cell concentration of 25,000 cells/well. Cell lysates were prepared and analyzed in the HPV18 E7 ELISA using biotinylated RabMAb 143-7. Results of one representative experiment are represented in nonlogarithmic (left) and semilogarithmic (right) graphs. The detection limit for HeLa cells was determined to be roughly 500 to 1,000 cells/well. (B) HPV18-positive HeLa cells and HPV-negative U-2OS cells were analyzed in various compositions, as indicated. Cellular lysates were prepared, and an aliquot was analyzed by the HPV18 E7 ELISA (left). <t>DNA-based</t> fluorescence of the same lysates was determined after addition of <t>EvaGreen</t> (right). Note that the signal obtained with the E7 ELISA is linearly decreasing with the number of HeLa cells, whereas the overall cell number is appropriately estimated by the EvaGreen signal. (C) Different amounts of HPV18-positive HeLa, HPV16-positive CaSki, and HPV-negative C33a cells were used for generation of cellular lysates, which were subsequently analyzed by 18E7 ELISA, implicating biotinylated RabMAb 143-7. (D) Lysates were prepared from 24 HPV DNA-negative cervical smears, all classified PapII. Patients were numbered from 15 to 38, as indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The red line indicates the cutoff value, defined as the average ELISA signal of all 24 patients displayed, plus 3 SDs. (E) Lysates were prepared from 14 HPV18 DNA-positive cervical smear specimens. Patients were numbered from 1 to 14, as indicated. For each sample, the cytological assessment at the time of diagnosis is indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The cutoff value determined for panel D is shown here for reference.
    Evagreen Dna Stain Solution, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/evagreen dna stain solution/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    evagreen dna stain solution - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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    E7 detection in cervical carcinoma cells and cervical smears. (A) An increasing number of HPV18-positive HeLa cells (0 to 25,000 cells/well) was complemented with HPV-negative U-2OS cells to achieve a total cell concentration of 25,000 cells/well. Cell lysates were prepared and analyzed in the HPV18 E7 ELISA using biotinylated RabMAb 143-7. Results of one representative experiment are represented in nonlogarithmic (left) and semilogarithmic (right) graphs. The detection limit for HeLa cells was determined to be roughly 500 to 1,000 cells/well. (B) HPV18-positive HeLa cells and HPV-negative U-2OS cells were analyzed in various compositions, as indicated. Cellular lysates were prepared, and an aliquot was analyzed by the HPV18 E7 ELISA (left). DNA-based fluorescence of the same lysates was determined after addition of EvaGreen (right). Note that the signal obtained with the E7 ELISA is linearly decreasing with the number of HeLa cells, whereas the overall cell number is appropriately estimated by the EvaGreen signal. (C) Different amounts of HPV18-positive HeLa, HPV16-positive CaSki, and HPV-negative C33a cells were used for generation of cellular lysates, which were subsequently analyzed by 18E7 ELISA, implicating biotinylated RabMAb 143-7. (D) Lysates were prepared from 24 HPV DNA-negative cervical smears, all classified PapII. Patients were numbered from 15 to 38, as indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The red line indicates the cutoff value, defined as the average ELISA signal of all 24 patients displayed, plus 3 SDs. (E) Lysates were prepared from 14 HPV18 DNA-positive cervical smear specimens. Patients were numbered from 1 to 14, as indicated. For each sample, the cytological assessment at the time of diagnosis is indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The cutoff value determined for panel D is shown here for reference.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection of Human Papillomavirus Type 18 E7 Oncoprotein in Cervical Smears: a Feasibility Study

    doi: 10.1128/JCM.01108-11

    Figure Lengend Snippet: E7 detection in cervical carcinoma cells and cervical smears. (A) An increasing number of HPV18-positive HeLa cells (0 to 25,000 cells/well) was complemented with HPV-negative U-2OS cells to achieve a total cell concentration of 25,000 cells/well. Cell lysates were prepared and analyzed in the HPV18 E7 ELISA using biotinylated RabMAb 143-7. Results of one representative experiment are represented in nonlogarithmic (left) and semilogarithmic (right) graphs. The detection limit for HeLa cells was determined to be roughly 500 to 1,000 cells/well. (B) HPV18-positive HeLa cells and HPV-negative U-2OS cells were analyzed in various compositions, as indicated. Cellular lysates were prepared, and an aliquot was analyzed by the HPV18 E7 ELISA (left). DNA-based fluorescence of the same lysates was determined after addition of EvaGreen (right). Note that the signal obtained with the E7 ELISA is linearly decreasing with the number of HeLa cells, whereas the overall cell number is appropriately estimated by the EvaGreen signal. (C) Different amounts of HPV18-positive HeLa, HPV16-positive CaSki, and HPV-negative C33a cells were used for generation of cellular lysates, which were subsequently analyzed by 18E7 ELISA, implicating biotinylated RabMAb 143-7. (D) Lysates were prepared from 24 HPV DNA-negative cervical smears, all classified PapII. Patients were numbered from 15 to 38, as indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The red line indicates the cutoff value, defined as the average ELISA signal of all 24 patients displayed, plus 3 SDs. (E) Lysates were prepared from 14 HPV18 DNA-positive cervical smear specimens. Patients were numbered from 1 to 14, as indicated. For each sample, the cytological assessment at the time of diagnosis is indicated. Lysates were analyzed by the HPV18 E7 ELISA and EvaGreen assay in parallel. Shown in the figure are normalized values. The cutoff value determined for panel D is shown here for reference.

    Article Snippet: The autofluorescence of the lysate was measured, 2 μl 50× EvaGreen DNA stain solution (Jena Bioscience, Jena, Germany) was added, and the plate was incubated for 10 min in the dark at room temperature.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence