qpcr probesmaster  (Jena Bioscience)


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    Name:
    qPCR ProbesMaster
    Description:
    qPCR ProbesMaster is designed for quantitative real time analysis of DNA samples using DNA probe based detection The master mix is recommended for use with Dual Labeled Fluorescent Probes e g TaqMan Molecular Beacons or FRET probes It provides an easy to handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision The mix contains all reagents required for qPCR except template primer and labeled fluorescent probe in a premixed 2x concentrated ready to use solution The high specificity and sensitivity of the mix based on an optimized hot start polymerase Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation The thermal activation prevents the extension of nonspecifically annealed primers and primer dimer formation at low temperatures during PCR setup The mix can also be used in combination with ROX reference dye PCR 351 in PCR instruments that are compatible with the evaluation of the ROX signal Dual labeled DNA probes Real time PCR technology based on dual labeled DNA probes provides a high sensitive and high specific PCR system with multiplexing capability It requires two standard PCR primers and the DNA probe that hybridizes to an internal part of the amplicon The sequence of the dual labeled DNA probe should avoid secondary structure and primer dimer formation
    Catalog Number:
    pcr-360l
    Price:
    476.96
    Category:
    Molecular Biology
    Format:
    liquid
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    Structured Review

    Jena Bioscience qpcr probesmaster
    qPCR ProbesMaster is designed for quantitative real time analysis of DNA samples using DNA probe based detection The master mix is recommended for use with Dual Labeled Fluorescent Probes e g TaqMan Molecular Beacons or FRET probes It provides an easy to handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision The mix contains all reagents required for qPCR except template primer and labeled fluorescent probe in a premixed 2x concentrated ready to use solution The high specificity and sensitivity of the mix based on an optimized hot start polymerase Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation The thermal activation prevents the extension of nonspecifically annealed primers and primer dimer formation at low temperatures during PCR setup The mix can also be used in combination with ROX reference dye PCR 351 in PCR instruments that are compatible with the evaluation of the ROX signal Dual labeled DNA probes Real time PCR technology based on dual labeled DNA probes provides a high sensitive and high specific PCR system with multiplexing capability It requires two standard PCR primers and the DNA probe that hybridizes to an internal part of the amplicon The sequence of the dual labeled DNA probe should avoid secondary structure and primer dimer formation
    https://www.bioz.com/result/qpcr probesmaster/product/Jena Bioscience
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    qpcr probesmaster - by Bioz Stars, 2020-07
    92/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Higher Prevalence and Abundance of Bdellovibrio bacteriovorus in the Human Gut of Healthy Subjects
    Article Snippet: .. The qPCR reaction mix was made up with 10 µl of ‘2X qPCR ProbesMaster’ from Jena Bioscience GmbH (Jena, Germany) (composition: qPCR Pol, dATP, dCTP, dGTP, dUTP, reaction buffer with KCl, (NH4 )2 SO4 and MgCl2 , ROX, stabilizers), 900 nM for both of the primers, 50 nM for the probe, and 5 µl of template (corresponding to 200 ng of total DNA). .. The final reaction volume was adjusted to 20 µl with PCR-grade distilled water.

    Article Title: Antibodies to Interferon beta in Patients with Multiple Sclerosis Receiving CinnoVex, Rebif, and Betaferon
    Article Snippet: .. The sequence of the GAPDH and MxA probes was as follows: GAPDH probe: Hex-AGC GCC AGC ATC GCC CCA C-BHQ-1 MxA probe: Hex-CCA GAT GGA ACA GAT TGT CTA CTG CCA G-BHQ-1 All real-time PCR reactions were conducted in 25 µL volumes using a qPCR ProbesMaster (Jena Bioscience, GmbH) and a Rotor-Gene 6000 (Corbett Life Science, Sydney, Australia). .. The transcriptional expression of MxA was normalized with an internal control of choice to avoid differences due to possible RNA degradation/contamination or different reverse transcription efficiency.

    Article Title: The microbiota of hematophagous ectoparasites collected from migratory birds
    Article Snippet: .. Briefly, the reaction mix was composed of 12.5 μl SuperMix PCR-UDG 2X (qPCR ProbesMaster, Jena Bioscience, Jena, Germany), 0.38 μl primer LC01490 20 μM, 0.38 μl primer HC02198 20 μM, 11 μl H2O, 0.75 μl cDNA, for a total volume of 20 μl. .. The thermal profile was: 50°C x 2 m; 95°C x 2 m; 40 cycles {94°C x 30 s, 49°C x 30 s, 72°C x 1 m}; 72°C x 5 m. Successful amplification was verified using E-Gel® precast agarose gels at 2% (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Antibodies to Interferon beta in Patients with Multiple Sclerosis Receiving CinnoVex, Rebif, and Betaferon
    Article Snippet: .. The sequence of the GAPDH and MxA probes was as follows: GAPDH probe: Hex-AGC GCC AGC ATC GCC CCA C-BHQ-1 MxA probe: Hex-CCA GAT GGA ACA GAT TGT CTA CTG CCA G-BHQ-1 All real-time PCR reactions were conducted in 25 µL volumes using a qPCR ProbesMaster (Jena Bioscience, GmbH) and a Rotor-Gene 6000 (Corbett Life Science, Sydney, Australia). .. The transcriptional expression of MxA was normalized with an internal control of choice to avoid differences due to possible RNA degradation/contamination or different reverse transcription efficiency.

    CTG Assay:

    Article Title: Antibodies to Interferon beta in Patients with Multiple Sclerosis Receiving CinnoVex, Rebif, and Betaferon
    Article Snippet: .. The sequence of the GAPDH and MxA probes was as follows: GAPDH probe: Hex-AGC GCC AGC ATC GCC CCA C-BHQ-1 MxA probe: Hex-CCA GAT GGA ACA GAT TGT CTA CTG CCA G-BHQ-1 All real-time PCR reactions were conducted in 25 µL volumes using a qPCR ProbesMaster (Jena Bioscience, GmbH) and a Rotor-Gene 6000 (Corbett Life Science, Sydney, Australia). .. The transcriptional expression of MxA was normalized with an internal control of choice to avoid differences due to possible RNA degradation/contamination or different reverse transcription efficiency.

    Polymerase Chain Reaction:

    Article Title: The microbiota of hematophagous ectoparasites collected from migratory birds
    Article Snippet: .. Briefly, the reaction mix was composed of 12.5 μl SuperMix PCR-UDG 2X (qPCR ProbesMaster, Jena Bioscience, Jena, Germany), 0.38 μl primer LC01490 20 μM, 0.38 μl primer HC02198 20 μM, 11 μl H2O, 0.75 μl cDNA, for a total volume of 20 μl. .. The thermal profile was: 50°C x 2 m; 95°C x 2 m; 40 cycles {94°C x 30 s, 49°C x 30 s, 72°C x 1 m}; 72°C x 5 m. Successful amplification was verified using E-Gel® precast agarose gels at 2% (Thermo Fisher Scientific).

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  • 92
    Jena Bioscience qpcr probesmaster
    Qpcr Probesmaster, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr probesmaster/product/Jena Bioscience
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    qpcr probesmaster - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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