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Illumina Inc pcr primer cocktail
The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr primer cocktail/product/Illumina Inc
Average 92 stars, based on 140 article reviews
Price from $9.99 to $1999.99
pcr primer cocktail - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA"

Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA

Journal: Epigenetics & Chromatin

doi: 10.1186/s13072-017-0123-7

The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
Figure Legend Snippet: The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

Techniques Used: Polymerase Chain Reaction, Amplification

2) Product Images from "MTHFD1 controls DNA methylation in Arabidopsis"

Article Title: MTHFD1 controls DNA methylation in Arabidopsis

Journal: Nature Communications

doi: 10.1038/ncomms11640

SDCpro-GFP expression and DNA demethylation caused by R175Q mutation in MTHFD1 . ( a ) GFP fluorescence micrographs of WT, #162 M2, MTHFD1/ mthfd1-2 F1 and #162/ mthfd1-2 F1 seedlings. F1 are progeny of #162 M2 x MTHFD1/mthfd1-2 . Dashed boxes indicate magnified areas shown in lower panels. Inlets show bright-field images. ( b ) Gene structure, positions of mutations and conserved domains of MTHFD1. The EMS mutation in #162 lead to a R175Q substitution of a conserved residue required for NADP binding 28 . ( c ) PCR-based genotype analysis of 13 F1 seedlings and two control samples. Arrowheads mark bands corresponding to WT/ mthfd1-1 (upper) and mthfd1-2 (lower). The mthfd1-2 allele co-segregates with GFP fluorescence in F1 (+: present, −: absent). L, ladder. ( d ) Habit of different genotype plants 20 days after germination. Scale bar, 10 mm. ( e ) DNA blot analysis of non-CG methylation at the MEA-ISR locus. Genomic DNA was digested with methylation-sensitive MspI; upper and lower bands correspond to methylated (m) and unmethylated (u) fragments, respectively. Ratios of band intensities for each lane are shown under the gel image. ( f ) Levels of non-CG methylation at the AtSN1 locus by quantitative chop PCR analysis of genomic DNA after digestion with methylation-sensitive HaeIII relative to undigested DNA. Mean values±s.d. ( n =3). Different letters above bars indicate significant differences between pairwise comparisons by Student's t -test ( P
Figure Legend Snippet: SDCpro-GFP expression and DNA demethylation caused by R175Q mutation in MTHFD1 . ( a ) GFP fluorescence micrographs of WT, #162 M2, MTHFD1/ mthfd1-2 F1 and #162/ mthfd1-2 F1 seedlings. F1 are progeny of #162 M2 x MTHFD1/mthfd1-2 . Dashed boxes indicate magnified areas shown in lower panels. Inlets show bright-field images. ( b ) Gene structure, positions of mutations and conserved domains of MTHFD1. The EMS mutation in #162 lead to a R175Q substitution of a conserved residue required for NADP binding 28 . ( c ) PCR-based genotype analysis of 13 F1 seedlings and two control samples. Arrowheads mark bands corresponding to WT/ mthfd1-1 (upper) and mthfd1-2 (lower). The mthfd1-2 allele co-segregates with GFP fluorescence in F1 (+: present, −: absent). L, ladder. ( d ) Habit of different genotype plants 20 days after germination. Scale bar, 10 mm. ( e ) DNA blot analysis of non-CG methylation at the MEA-ISR locus. Genomic DNA was digested with methylation-sensitive MspI; upper and lower bands correspond to methylated (m) and unmethylated (u) fragments, respectively. Ratios of band intensities for each lane are shown under the gel image. ( f ) Levels of non-CG methylation at the AtSN1 locus by quantitative chop PCR analysis of genomic DNA after digestion with methylation-sensitive HaeIII relative to undigested DNA. Mean values±s.d. ( n =3). Different letters above bars indicate significant differences between pairwise comparisons by Student's t -test ( P

Techniques Used: Expressing, Mutagenesis, Fluorescence, Binding Assay, Polymerase Chain Reaction, Methylation, Microelectrode Array

3) Product Images from "Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation"

Article Title: Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation

Journal: PLoS ONE

doi: 10.1371/journal.pone.0159723

(A) Primer sequences used in quantitative real-time reverse transcription PCR (qRT-PCR) analysis for validation of the expressed genes in Illumina sequencing. (B) Comparison between the gene expression ratios obtained from RNA-seq data and that from qRT-PCR. The RNA-seq log2 value of the expression ratio (y-axis) was plotted against the developmental stages (x-axis). (C) qRT-PCR analysis of differentially expressed genes between cgy-2 -NIL and DN47. The transcript abundance from the RNA-seq data is shown at the top of the panel for each gene. RPKM: reads per kilobase per million reads. DAF = days after flowering.
Figure Legend Snippet: (A) Primer sequences used in quantitative real-time reverse transcription PCR (qRT-PCR) analysis for validation of the expressed genes in Illumina sequencing. (B) Comparison between the gene expression ratios obtained from RNA-seq data and that from qRT-PCR. The RNA-seq log2 value of the expression ratio (y-axis) was plotted against the developmental stages (x-axis). (C) qRT-PCR analysis of differentially expressed genes between cgy-2 -NIL and DN47. The transcript abundance from the RNA-seq data is shown at the top of the panel for each gene. RPKM: reads per kilobase per million reads. DAF = days after flowering.

Techniques Used: Polymerase Chain Reaction, Quantitative RT-PCR, Sequencing, Expressing, RNA Sequencing Assay

Related Articles

Polymerase Chain Reaction:

Article Title: Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing
Article Snippet: .. DNA fragments with adaptors ligated on both ends were selectively enriched using the Illumina PCR Primer Cocktail with a 10-cycle PCR protocol. .. The products were then purified (AMPure XP system) and quantified using Agilent high-sensitivity DNA assays on the Agilent Bioanalyzer 2100 system.

Article Title: Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine. Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine
Article Snippet: .. Illumina PCR Primer Cocktail in a 15 cycle PCR was used to selectively enrich the DNA fragments with ligated adaptor molecules on both ends. .. Besides, purification and quantification of products were conducted by AMPure XP system (Beckman Coulter) and Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent), respectively.

Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA
Article Snippet: .. After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B). .. The KRuO4 -mediated 5mC-to-5caC/(T) oxidation efficiency was 99.33% as estimated using 5hmC-to-5caC/(T) conversion efficiency of 5hmC pUC18 control in OxBis reaction normalized to that in the conventional bisulphite reaction.

Article Title: Molecular Mechanism for Stress-Induced Depression Assessed by Sequencing miRNA and mRNA in Medial Prefrontal Cortex
Article Snippet: .. After the amplification with Illumina PCR Primer Cocktail within 15 cycles of PCR reaction, these cDNAs were size-selected and purified by agarose gel electrophoresis. cDNAs with the sizes between 200 and 300 bp were selected for the library construction. .. In the meantime, the miRNA sequencing library was constructed from those total RNAs, in which low molecular weight RNAs (18–30 nt) were isolated by the polyacrylamide gel electrophoresis.

Article Title: Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection
Article Snippet: .. DNA fragments with ligated adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 10 cycle PCR reaction and then purified using AMPure XP system. .. The quality of library was assessed on the Agilent Bioanalyzer 2100 system and the clustering of the index-coded sample was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the vender’s instructions.

Article Title: Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation
Article Snippet: .. DNA fragments with ligated adaptor molecules on both ends were enriched selectively using the Illumina PCR Primer Cocktail in a 10-cycle PCR reaction. .. Products were purified using the AMPure XP system and quantified using the Agilent high-sensitivity DNA assay on the Agilent Bioanalyzer 2100 system. cDNA Library concentration was first quantified using a Qubit 2.0 fluorometer (Life Technologies), and then diluted to 1 ng/μl before checking insert size on an Agilent 2100 and quantifying to greater accuracy by quantitative PCR (Q-PCR) (library activity > 2 nM).The library preparations were sequenced on an Illumina Hiseq 2000 platform and 100-bp paired-end reads were generated.

Article Title: Transcriptome analysis of rice (Oryza sativa L.) shoots responsive to cadmium stress
Article Snippet: .. DNA fragments with ligated adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. .. Products were purified (AMPure XP system) and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent).

Article Title: MTHFD1 controls DNA methylation in Arabidopsis
Article Snippet: .. Libraries were amplified using PCR primer cocktail (Illumina) and Pfu Turbo Cx hotstart DNA polymerase (Agilent). .. Sequencing was performed on a HiSeq 2000 platform at 50 bp length.

Sequencing:

Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA
Article Snippet: .. After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B). .. The KRuO4 -mediated 5mC-to-5caC/(T) oxidation efficiency was 99.33% as estimated using 5hmC-to-5caC/(T) conversion efficiency of 5hmC pUC18 control in OxBis reaction normalized to that in the conventional bisulphite reaction.

Amplification:

Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA
Article Snippet: .. After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B). .. The KRuO4 -mediated 5mC-to-5caC/(T) oxidation efficiency was 99.33% as estimated using 5hmC-to-5caC/(T) conversion efficiency of 5hmC pUC18 control in OxBis reaction normalized to that in the conventional bisulphite reaction.

Article Title: Molecular Mechanism for Stress-Induced Depression Assessed by Sequencing miRNA and mRNA in Medial Prefrontal Cortex
Article Snippet: .. After the amplification with Illumina PCR Primer Cocktail within 15 cycles of PCR reaction, these cDNAs were size-selected and purified by agarose gel electrophoresis. cDNAs with the sizes between 200 and 300 bp were selected for the library construction. .. In the meantime, the miRNA sequencing library was constructed from those total RNAs, in which low molecular weight RNAs (18–30 nt) were isolated by the polyacrylamide gel electrophoresis.

Article Title: MTHFD1 controls DNA methylation in Arabidopsis
Article Snippet: .. Libraries were amplified using PCR primer cocktail (Illumina) and Pfu Turbo Cx hotstart DNA polymerase (Agilent). .. Sequencing was performed on a HiSeq 2000 platform at 50 bp length.

Agarose Gel Electrophoresis:

Article Title: Molecular Mechanism for Stress-Induced Depression Assessed by Sequencing miRNA and mRNA in Medial Prefrontal Cortex
Article Snippet: .. After the amplification with Illumina PCR Primer Cocktail within 15 cycles of PCR reaction, these cDNAs were size-selected and purified by agarose gel electrophoresis. cDNAs with the sizes between 200 and 300 bp were selected for the library construction. .. In the meantime, the miRNA sequencing library was constructed from those total RNAs, in which low molecular weight RNAs (18–30 nt) were isolated by the polyacrylamide gel electrophoresis.

Purification:

Article Title: Molecular Mechanism for Stress-Induced Depression Assessed by Sequencing miRNA and mRNA in Medial Prefrontal Cortex
Article Snippet: .. After the amplification with Illumina PCR Primer Cocktail within 15 cycles of PCR reaction, these cDNAs were size-selected and purified by agarose gel electrophoresis. cDNAs with the sizes between 200 and 300 bp were selected for the library construction. .. In the meantime, the miRNA sequencing library was constructed from those total RNAs, in which low molecular weight RNAs (18–30 nt) were isolated by the polyacrylamide gel electrophoresis.

Article Title: Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection
Article Snippet: .. DNA fragments with ligated adaptor molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 10 cycle PCR reaction and then purified using AMPure XP system. .. The quality of library was assessed on the Agilent Bioanalyzer 2100 system and the clustering of the index-coded sample was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the vender’s instructions.

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    Illumina Inc pcr primer cocktail
    The workflow of conventional bisulphite (Bis), oxidative bisulphite <t>(OxBis)</t> and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after <t>PCR</t> amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC
    Pcr Primer Cocktail, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer cocktail/product/Illumina Inc
    Average 92 stars, based on 140 article reviews
    Price from $9.99 to $1999.99
    pcr primer cocktail - by Bioz Stars, 2020-07
    92/100 stars
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    The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Journal: Epigenetics & Chromatin

    Article Title: Comprehensive evaluation of genome-wide 5-hydroxymethylcytosine profiling approaches in human DNA

    doi: 10.1186/s13072-017-0123-7

    Figure Lengend Snippet: The workflow of conventional bisulphite (Bis), oxidative bisulphite (OxBis) and TET-assisted bisulphite (TAB) approaches for the detection of 5mC and 5hmC. Bisulphite treatment alone results in the conversion of unmethylated cytosine into uracil that will be read as thymine after PCR amplification, with both 5mC and 5hmC being read as cytosine. The readout of Bis is denoted as 5modC (5mC + 5hmC). The addition of an oxidation step prior to bisulphite treatment results in the conversion of 5hmC to 5fC that will be converted to uracil together with an unmethylated cytosine. Thus, the readout of OxBis is 5mC. In TAB, the first step involves β-glucosyltransferase-mediated protection of 5hmC with a glucose moiety, followed by TET-mediated oxidation of 5mC to 5caC, which will be converted to uracil. Thus, the readout of TAB is 5hmC

    Article Snippet: After the completion of the OxBis reaction, 5hmC APC PCR product was amplified using PCR primer cocktail supplied by Illumina followed by the clonal Sanger sequencing as described for the M.SssI λDNA control (Additional file : Figure S1B).

    Techniques: Polymerase Chain Reaction, Amplification

    SDCpro-GFP expression and DNA demethylation caused by R175Q mutation in MTHFD1 . ( a ) GFP fluorescence micrographs of WT, #162 M2, MTHFD1/ mthfd1-2 F1 and #162/ mthfd1-2 F1 seedlings. F1 are progeny of #162 M2 x MTHFD1/mthfd1-2 . Dashed boxes indicate magnified areas shown in lower panels. Inlets show bright-field images. ( b ) Gene structure, positions of mutations and conserved domains of MTHFD1. The EMS mutation in #162 lead to a R175Q substitution of a conserved residue required for NADP binding 28 . ( c ) PCR-based genotype analysis of 13 F1 seedlings and two control samples. Arrowheads mark bands corresponding to WT/ mthfd1-1 (upper) and mthfd1-2 (lower). The mthfd1-2 allele co-segregates with GFP fluorescence in F1 (+: present, −: absent). L, ladder. ( d ) Habit of different genotype plants 20 days after germination. Scale bar, 10 mm. ( e ) DNA blot analysis of non-CG methylation at the MEA-ISR locus. Genomic DNA was digested with methylation-sensitive MspI; upper and lower bands correspond to methylated (m) and unmethylated (u) fragments, respectively. Ratios of band intensities for each lane are shown under the gel image. ( f ) Levels of non-CG methylation at the AtSN1 locus by quantitative chop PCR analysis of genomic DNA after digestion with methylation-sensitive HaeIII relative to undigested DNA. Mean values±s.d. ( n =3). Different letters above bars indicate significant differences between pairwise comparisons by Student's t -test ( P

    Journal: Nature Communications

    Article Title: MTHFD1 controls DNA methylation in Arabidopsis

    doi: 10.1038/ncomms11640

    Figure Lengend Snippet: SDCpro-GFP expression and DNA demethylation caused by R175Q mutation in MTHFD1 . ( a ) GFP fluorescence micrographs of WT, #162 M2, MTHFD1/ mthfd1-2 F1 and #162/ mthfd1-2 F1 seedlings. F1 are progeny of #162 M2 x MTHFD1/mthfd1-2 . Dashed boxes indicate magnified areas shown in lower panels. Inlets show bright-field images. ( b ) Gene structure, positions of mutations and conserved domains of MTHFD1. The EMS mutation in #162 lead to a R175Q substitution of a conserved residue required for NADP binding 28 . ( c ) PCR-based genotype analysis of 13 F1 seedlings and two control samples. Arrowheads mark bands corresponding to WT/ mthfd1-1 (upper) and mthfd1-2 (lower). The mthfd1-2 allele co-segregates with GFP fluorescence in F1 (+: present, −: absent). L, ladder. ( d ) Habit of different genotype plants 20 days after germination. Scale bar, 10 mm. ( e ) DNA blot analysis of non-CG methylation at the MEA-ISR locus. Genomic DNA was digested with methylation-sensitive MspI; upper and lower bands correspond to methylated (m) and unmethylated (u) fragments, respectively. Ratios of band intensities for each lane are shown under the gel image. ( f ) Levels of non-CG methylation at the AtSN1 locus by quantitative chop PCR analysis of genomic DNA after digestion with methylation-sensitive HaeIII relative to undigested DNA. Mean values±s.d. ( n =3). Different letters above bars indicate significant differences between pairwise comparisons by Student's t -test ( P

    Article Snippet: Libraries were amplified using PCR primer cocktail (Illumina) and Pfu Turbo Cx hotstart DNA polymerase (Agilent).

    Techniques: Expressing, Mutagenesis, Fluorescence, Binding Assay, Polymerase Chain Reaction, Methylation, Microelectrode Array

    (A) Primer sequences used in quantitative real-time reverse transcription PCR (qRT-PCR) analysis for validation of the expressed genes in Illumina sequencing. (B) Comparison between the gene expression ratios obtained from RNA-seq data and that from qRT-PCR. The RNA-seq log2 value of the expression ratio (y-axis) was plotted against the developmental stages (x-axis). (C) qRT-PCR analysis of differentially expressed genes between cgy-2 -NIL and DN47. The transcript abundance from the RNA-seq data is shown at the top of the panel for each gene. RPKM: reads per kilobase per million reads. DAF = days after flowering.

    Journal: PLoS ONE

    Article Title: Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation

    doi: 10.1371/journal.pone.0159723

    Figure Lengend Snippet: (A) Primer sequences used in quantitative real-time reverse transcription PCR (qRT-PCR) analysis for validation of the expressed genes in Illumina sequencing. (B) Comparison between the gene expression ratios obtained from RNA-seq data and that from qRT-PCR. The RNA-seq log2 value of the expression ratio (y-axis) was plotted against the developmental stages (x-axis). (C) qRT-PCR analysis of differentially expressed genes between cgy-2 -NIL and DN47. The transcript abundance from the RNA-seq data is shown at the top of the panel for each gene. RPKM: reads per kilobase per million reads. DAF = days after flowering.

    Article Snippet: DNA fragments with ligated adaptor molecules on both ends were enriched selectively using the Illumina PCR Primer Cocktail in a 10-cycle PCR reaction.

    Techniques: Polymerase Chain Reaction, Quantitative RT-PCR, Sequencing, Expressing, RNA Sequencing Assay