pcr polymerase chain reaction clean up  (Beckman Coulter)


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    Beckman Coulter pcr polymerase chain reaction clean up
    Pcr Polymerase Chain Reaction Clean Up, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr polymerase chain reaction clean up/product/Beckman Coulter
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr polymerase chain reaction clean up - by Bioz Stars, 2020-08
    85/100 stars

    Related Products / Commonly Used Together

    beckman-coulter biomek nxp
    thermocyclers

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    Related Articles

    Sequencing:

    Article Title: Identification of low-frequency TRAF3IP2 coding variants in psoriatic arthritis patients and functional characterization
    Article Snippet: .. In general, Sanger sequencing was performed, as described before [ ], with the following changes: thermocyclers were Thermo Multiblock (ThermoFisher Scientific, Ulm, Germany); the robotic system for PCR (polymerase chain reaction) clean-up as well as for sequencing reactions was Beckman-Coulter Biomek Nxp (kit for PCR clean-up: Agencourt AMPure; kit for sequencing reactions: CleanSEQ; Beckman-Coulter, Krefeld, Germany). .. Sequences were analyzed with the Software Sequencher versus 4.10.1 (Gene Codes, Ann Arbor, MI, USA).

    Polymerase Chain Reaction:

    Article Title: Identification of low-frequency TRAF3IP2 coding variants in psoriatic arthritis patients and functional characterization
    Article Snippet: .. In general, Sanger sequencing was performed, as described before [ ], with the following changes: thermocyclers were Thermo Multiblock (ThermoFisher Scientific, Ulm, Germany); the robotic system for PCR (polymerase chain reaction) clean-up as well as for sequencing reactions was Beckman-Coulter Biomek Nxp (kit for PCR clean-up: Agencourt AMPure; kit for sequencing reactions: CleanSEQ; Beckman-Coulter, Krefeld, Germany). .. Sequences were analyzed with the Software Sequencher versus 4.10.1 (Gene Codes, Ann Arbor, MI, USA).

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    Beckman Coulter pcr clean up
    Library QC Tape station electropherogram. <t>Nextera</t> <t>Post-PCR</t> libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Pcr Clean Up, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 3711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up/product/Beckman Coulter
    Average 93 stars, based on 3711 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2020-08
    93/100 stars
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    Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Journal: BMC Biotechnology

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system

    doi: 10.1186/1472-6750-13-104

    Figure Lengend Snippet: Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Article Snippet: Thermocycling was carried out on a Tetrad (Bio-Rad, 1000 Alfred Nobel Drive, Hercules, CA, 94547, USA) with the following standard Nextera parameters: PCR clean-up was performed following the Nextera protocol using a 0.6:1 ratio of AMPure XP® (Beckman Coulter) to PCR reaction.

    Techniques: Polymerase Chain Reaction, Construct, Produced