rnaclean xp  (Beckman Coulter)


Bioz Verified Symbol Beckman Coulter is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Beckman Coulter rnaclean xp
    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, <t>RNAClean™</t> XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Rnaclean Xp, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 7871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaclean xp/product/Beckman Coulter
    Average 99 stars, based on 7871 article reviews
    Price from $9.99 to $1999.99
    rnaclean xp - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application"

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    Journal: BMC Genomics

    doi: 10.1186/s12864-017-4371-5

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Figure Legend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Techniques Used: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    2) Product Images from "A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice"

    Article Title: A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

    Journal: Nature Communications

    doi: 10.1038/s41467-020-17962-3

    The C olor A ssay T racing- R epair (CAT-R) reporter system. a Representation of the CAT-R reporter showing the different DNA repair outcomes after a Cas9-mediated site-specific double-strand break (DSB). The CRISPR/Cas9 target site is indicated at the eGFP locus 355 bp downstream of the P2A peptide. If the break resolves through repair with small InDels, frameshift mutations will translate eGFP out of frame, and only the mCherry will express; if the break resolves with large deletions both mCherry and eGFP sequences will be lost. For simplicity, we refer to the double negative population as large deletions, but they likely include additional classes of rearrangements. b Flow cytometry plots of HEK293 CAT-R and RPE-1 CAT-R cells 72 h post-transfection with a non-targeting (scrambled) gRNA as control and a gRNA targeting the eGFP coding sequence. Numbers inside plots indicate percentages of live cells. Axes show fluorescence intensities of eGFP and mCherry proteins. c Overlay of flow cytometry plots, monitoring the fluorescent intensity of HEK293 CAT-R cells for 2, 5, and 7 days following DSB induction. d Box and whisker plot ( n = 45, centerlines mark the medians, box limits indicate the 25th and 75th percentiles, and whiskers extend to min and max, showing all points) of flow cytometry analysis for HEK293 CAT-R and RPE-1 CAT-R cell lines 72 h post-transfection with the synthetic gRNA. The p -values are calculated with a multiple comparison analysis testing in ANOVA followed by a Dunnett’s test. Data are derived from 15 independent experiments; n represents the number of all replicates. e Short-read PCR amplicon sequencing from genomic DNA harvested 72 h post-transfection to detect small InDels at the targeted site. Y axis shows the frequency of events and the X axis shows the length of detected deletions or insertions at the break site. f Long-read PCR amplicon sequencing from genomic DNA harvested 72 h post-transfection to detect large deletions at the targeted site. Y axis shows the coverage of events and the X axis shows the length of detected deletions at the break site. Bar plots show the frequency of events per sample.
    Figure Legend Snippet: The C olor A ssay T racing- R epair (CAT-R) reporter system. a Representation of the CAT-R reporter showing the different DNA repair outcomes after a Cas9-mediated site-specific double-strand break (DSB). The CRISPR/Cas9 target site is indicated at the eGFP locus 355 bp downstream of the P2A peptide. If the break resolves through repair with small InDels, frameshift mutations will translate eGFP out of frame, and only the mCherry will express; if the break resolves with large deletions both mCherry and eGFP sequences will be lost. For simplicity, we refer to the double negative population as large deletions, but they likely include additional classes of rearrangements. b Flow cytometry plots of HEK293 CAT-R and RPE-1 CAT-R cells 72 h post-transfection with a non-targeting (scrambled) gRNA as control and a gRNA targeting the eGFP coding sequence. Numbers inside plots indicate percentages of live cells. Axes show fluorescence intensities of eGFP and mCherry proteins. c Overlay of flow cytometry plots, monitoring the fluorescent intensity of HEK293 CAT-R cells for 2, 5, and 7 days following DSB induction. d Box and whisker plot ( n = 45, centerlines mark the medians, box limits indicate the 25th and 75th percentiles, and whiskers extend to min and max, showing all points) of flow cytometry analysis for HEK293 CAT-R and RPE-1 CAT-R cell lines 72 h post-transfection with the synthetic gRNA. The p -values are calculated with a multiple comparison analysis testing in ANOVA followed by a Dunnett’s test. Data are derived from 15 independent experiments; n represents the number of all replicates. e Short-read PCR amplicon sequencing from genomic DNA harvested 72 h post-transfection to detect small InDels at the targeted site. Y axis shows the frequency of events and the X axis shows the length of detected deletions or insertions at the break site. f Long-read PCR amplicon sequencing from genomic DNA harvested 72 h post-transfection to detect large deletions at the targeted site. Y axis shows the coverage of events and the X axis shows the length of detected deletions at the break site. Bar plots show the frequency of events per sample.

    Techniques Used: CRISPR, Flow Cytometry, Transfection, Sequencing, Fluorescence, Whisker Assay, Derivative Assay, Polymerase Chain Reaction, Amplification

    3) Product Images from "Rapid Genomic Characterization of SARS-CoV-2 by Direct Amplicon-Based Sequencing Through Comparison of MinION and Illumina iSeq100TM System"

    Article Title: Rapid Genomic Characterization of SARS-CoV-2 by Direct Amplicon-Based Sequencing Through Comparison of MinION and Illumina iSeq100TM System

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.571328

    SARS-CoV-2 next-generation sequencing workflow, from the sample to sequence analysis. Created with biorender.com .
    Figure Legend Snippet: SARS-CoV-2 next-generation sequencing workflow, from the sample to sequence analysis. Created with biorender.com .

    Techniques Used: Next-Generation Sequencing, Sequencing

    4) Product Images from "Improved workflows for high throughput library preparation using the transposome-based nextera system"

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-13-104

    Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Figure Legend Snippet: Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Techniques Used: Polymerase Chain Reaction, Construct, Produced

    5) Product Images from "Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application"

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    Journal: BMC Genomics

    doi: 10.1186/s12864-017-4371-5

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown
    Figure Legend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Techniques Used: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    Related Articles

    RNA Extraction:

    Article Title: Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance
    Article Snippet: .. RNA extraction and quality assessment RNA was extracted from E8.5 embryos using the Genelute Mammalian total RNA miniprep kit (Sigma, RTN70-1KT), DNAse treated using TURBO DNA-free™ Kit (Ambion, AM1907) and purified from the DNAse reaction using Agencourt RNAClean XP (Beckman coulter, A63987). .. RNA was extracted from pools of eight to ten carefully staged eight-cell embryos, with each pool representing separate litters using Agencourt RNAClean XP (Beckman coulter, A63987) chemistry and the RNA DNAase treated on the beads, before elution, freezing and storage at − 80 °C.

    Selection:

    Article Title: R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes
    Article Snippet: .. Size selection was performed prior to PCR amplification using RNA clean XP beads (Cat. A63987, Beckman Coulter). .. Adaptors, PCR amplification and Index Primers were used to multiplex libraries (Multiplex oligos for Illlumina, Cat. E7335S and E7500S, NEB).

    RNA Sequencing Assay:

    Article Title: Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons
    Article Snippet: .. We annealed rRNA antisense oligos to total RNA samples for 2 minutes at 95°C, slowly reduced the temperature to 45°C and then added 2U of Hybridase Thermostable RNase H (Epicenter, Lucigen: H39500) and 1 μL of 10X Digestion buffer (500 mM Tris-HCl, 1 M NaCl, 200mM MgCl2) and incubated for 30 minutes at 45°C. rRNA-depleted RNA was then purified using 2.2X reaction volume of Agencourt RNAClean XP beads (Beckman Coulter: A63987), treated with TURBO DNase (Invitrogen: AM1907), and then purified with RNAClean XP beads again. rRNA-depleted RNA was used as input to the KAPA Stranded RNA-seq Kit (Kapa Biosystems: KK8400) to make RNA-sequencing libraries for fly knockdowns. .. For mouse primary neuron RNA-seq libraries, the KAPA HyperPrep RNA-seq Kit (Kapa Biosystems: KK8540) was used to create libraries after rRNA depletion using oligos antisense to human rRNA sequences ( ).

    Article Title: Widespread Transcriptional Scanning in the Testis Modulates Gene Evolution Rates.
    Article Snippet: .. The testis expresses the largest number of genes of any mammalian organ, a finding that has long puzzled molecular biologists. ..

    Magnetic Beads:

    Article Title: Widespread Transcriptional Scanning in the Testis Modulates Gene Evolution Rates.
    Article Snippet: .. The testis expresses the largest number of genes of any mammalian organ, a finding that has long puzzled molecular biologists. ..

    Purification:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons
    Article Snippet: .. We annealed rRNA antisense oligos to total RNA samples for 2 minutes at 95°C, slowly reduced the temperature to 45°C and then added 2U of Hybridase Thermostable RNase H (Epicenter, Lucigen: H39500) and 1 μL of 10X Digestion buffer (500 mM Tris-HCl, 1 M NaCl, 200mM MgCl2) and incubated for 30 minutes at 45°C. rRNA-depleted RNA was then purified using 2.2X reaction volume of Agencourt RNAClean XP beads (Beckman Coulter: A63987), treated with TURBO DNase (Invitrogen: AM1907), and then purified with RNAClean XP beads again. rRNA-depleted RNA was used as input to the KAPA Stranded RNA-seq Kit (Kapa Biosystems: KK8400) to make RNA-sequencing libraries for fly knockdowns. .. For mouse primary neuron RNA-seq libraries, the KAPA HyperPrep RNA-seq Kit (Kapa Biosystems: KK8540) was used to create libraries after rRNA depletion using oligos antisense to human rRNA sequences ( ).

    Article Title: The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti
    Article Snippet: .. Following transcription and DNAse treatment for 15 min at 37°C, sgRNA was purified using RNAse-free SPRI beads (Ampure RNAclean, Beckman-Coulter A63987), and eluted in Ultrapure water (Invitrogen, 10977–015). .. The donor plasmid was constructed by Gibson assembly using the following fragments: homology arms of ~1 kb on either side of the Cas9 cut site (two base pairs were deleted from the left arm immediately preceding the T2A to maintain the open reading frame); a fragment containing T2A-QF2-SV40 and 3xP3-dsRed , PCR-amplified from a vector derived from pBac-DsRed-ORCO_9kbProm-QF2 (gift of Chris Potter, Addgene #104877); a pUC57 vector backbone digested with EcoRI and HindIII.

    Article Title: Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance
    Article Snippet: .. RNA extraction and quality assessment RNA was extracted from E8.5 embryos using the Genelute Mammalian total RNA miniprep kit (Sigma, RTN70-1KT), DNAse treated using TURBO DNA-free™ Kit (Ambion, AM1907) and purified from the DNAse reaction using Agencourt RNAClean XP (Beckman coulter, A63987). .. RNA was extracted from pools of eight to ten carefully staged eight-cell embryos, with each pool representing separate litters using Agencourt RNAClean XP (Beckman coulter, A63987) chemistry and the RNA DNAase treated on the beads, before elution, freezing and storage at − 80 °C.

    Incubation:

    Article Title: Zinc Finger RNA-Binding Protein Zn72D Regulates ADAR-Mediated RNA Editing in Neurons
    Article Snippet: .. We annealed rRNA antisense oligos to total RNA samples for 2 minutes at 95°C, slowly reduced the temperature to 45°C and then added 2U of Hybridase Thermostable RNase H (Epicenter, Lucigen: H39500) and 1 μL of 10X Digestion buffer (500 mM Tris-HCl, 1 M NaCl, 200mM MgCl2) and incubated for 30 minutes at 45°C. rRNA-depleted RNA was then purified using 2.2X reaction volume of Agencourt RNAClean XP beads (Beckman Coulter: A63987), treated with TURBO DNase (Invitrogen: AM1907), and then purified with RNAClean XP beads again. rRNA-depleted RNA was used as input to the KAPA Stranded RNA-seq Kit (Kapa Biosystems: KK8400) to make RNA-sequencing libraries for fly knockdowns. .. For mouse primary neuron RNA-seq libraries, the KAPA HyperPrep RNA-seq Kit (Kapa Biosystems: KK8540) was used to create libraries after rRNA depletion using oligos antisense to human rRNA sequences ( ).

    Amplification:

    Article Title: R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes
    Article Snippet: .. Size selection was performed prior to PCR amplification using RNA clean XP beads (Cat. A63987, Beckman Coulter). .. Adaptors, PCR amplification and Index Primers were used to multiplex libraries (Multiplex oligos for Illlumina, Cat. E7335S and E7500S, NEB).

    DNA Purification:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Polymerase Chain Reaction:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Article Title: R-Loops Enhance Polycomb Repression at a Subset of Developmental Regulator Genes
    Article Snippet: .. Size selection was performed prior to PCR amplification using RNA clean XP beads (Cat. A63987, Beckman Coulter). .. Adaptors, PCR amplification and Index Primers were used to multiplex libraries (Multiplex oligos for Illlumina, Cat. E7335S and E7500S, NEB).

    Recombinant:

    Article Title: Widespread Transcriptional Scanning in the Testis Modulates Gene Evolution Rates.
    Article Snippet: .. The testis expresses the largest number of genes of any mammalian organ, a finding that has long puzzled molecular biologists. ..

    Chromatin Immunoprecipitation:

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
    Article Snippet: .. Similarly, DNAs were purified from de-crosslinked chromatin estimated to include 1 ng, 5 ng, 10 ng, and 50 ng of DNA after treatment of RNase A and proteinase K. The following reagents were used in the experiment: ChIP DNA Clean & Concentrator™ (Cat. # D5205) from Zymo Research (Zy) (Irvine, CA); Wizard® SV Gel and PCR Clean-Up System (Cat. # A9281) from Promega (Pr) (Fitchburg, WI); GeneJET PCR Purification Kit (Cat. # K0701) from Thermo Fisher Scientific (Th) (Waltham, MA); PureLink® PCR Purification Kit (Cat. # K310001) from Invitrogen (In) (Carlsbad, CA); Monarch® PCR & DNA Cleanup Kit (Cat. # T1030S) from New England Biolabs (Ne) (Ipswich, MA); Chromatin IP DNA Purification Kit (Cat. # 58002) from Active Motif (Am) (Carlsbad, CA); QIAquick PCR Purification Kit (Cat. # 28106) from Qiagen (Qp) (Valencia, CA), MinElute PCR Purification Kit (Cat. # 28006) from Qiagen (Qm); Agencourt AMPure XP (Cat. # A63881) from Beckman (Ba) (Indianapolis, IN), RNAClean™ XP (Cat. # A63987) from Beckman (Br), and phenol/chloroform extraction (PC) (Additional file ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Beckman Coulter pcr clean up
    Library QC Tape station electropherogram. <t>Nextera</t> <t>Post-PCR</t> libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Pcr Clean Up, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 3711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up/product/Beckman Coulter
    Average 92 stars, based on 3711 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    Image Search Results


    Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Journal: BMC Biotechnology

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system

    doi: 10.1186/1472-6750-13-104

    Figure Lengend Snippet: Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Article Snippet: Thermocycling was carried out on a Tetrad (Bio-Rad, 1000 Alfred Nobel Drive, Hercules, CA, 94547, USA) with the following standard Nextera parameters: PCR clean-up was performed following the Nextera protocol using a 0.6:1 ratio of AMPure XP® (Beckman Coulter) to PCR reaction.

    Techniques: Polymerase Chain Reaction, Construct, Produced